##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765531_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7924068
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40161051116674	33.0	31.0	34.0	31.0	34.0
2	32.53814694674503	34.0	31.0	34.0	31.0	34.0
3	32.58449763429592	34.0	31.0	34.0	31.0	34.0
4	36.127285757769876	37.0	35.0	37.0	35.0	37.0
5	36.056000907614624	37.0	35.0	37.0	35.0	37.0
6	36.11901803467613	37.0	36.0	37.0	35.0	37.0
7	36.064568476696564	37.0	35.0	37.0	35.0	37.0
8	36.07745251555136	37.0	36.0	37.0	35.0	37.0
9	37.79296000488638	39.0	38.0	39.0	35.0	39.0
10-11	37.73285009669276	39.0	38.0	39.0	35.0	39.0
12-13	37.699780340098044	39.0	38.0	39.0	35.0	39.0
14-15	39.02920513806797	40.0	38.0	41.0	36.0	41.0
16-17	39.002146372292614	40.0	38.0	41.0	36.0	41.0
18-19	39.028664380467205	40.0	38.5	41.0	35.5	41.0
20-21	39.00964971527251	40.0	39.0	41.0	35.0	41.0
22-23	38.94309740905807	40.0	38.0	41.0	35.0	41.0
24-25	38.87969695615939	40.0	38.0	41.0	35.0	41.0
26-27	38.73411163306524	40.0	38.0	41.0	35.0	41.0
28-29	38.58760191103862	40.0	38.0	41.0	34.0	41.0
30-31	38.39832192252767	40.0	38.0	41.0	34.0	41.0
32-33	38.126913663537465	40.0	37.0	41.0	34.0	41.0
34-35	38.0285222186382	40.0	37.0	41.0	33.5	41.0
36-37	37.903330839664676	40.0	37.0	41.0	33.0	41.0
38-39	37.72460691907237	40.0	37.0	41.0	33.0	41.0
40-41	37.48591171857687	40.0	37.0	41.0	33.0	41.0
42-43	37.220713464346844	40.0	36.0	41.0	32.5	41.0
44-45	36.88391688461028	39.5	36.0	41.0	31.5	41.0
46-47	36.52733741053207	39.0	35.0	41.0	31.0	41.0
48-49	36.20931729763046	39.0	35.0	41.0	30.0	41.0
50-51	35.9207511217723	39.0	35.0	40.0	29.5	41.0
52-53	35.778480384065354	39.0	35.0	40.0	29.0	41.0
54-55	35.7607283279245	39.0	35.0	41.0	29.0	41.0
56-57	35.580014078627286	39.0	35.0	40.5	29.0	41.0
58-59	35.447360938851105	38.0	35.0	40.5	29.0	41.0
60-61	35.12555287259019	37.5	35.0	40.0	28.0	41.0
62-63	34.775638661859034	37.0	35.0	40.0	28.0	41.0
64-65	34.560942372024066	36.5	35.0	39.5	28.0	41.0
66-67	34.21248909019963	36.0	34.0	39.0	28.0	41.0
68-69	33.875287541702065	35.5	34.0	39.0	27.5	41.0
70-71	33.501255037185445	35.0	34.0	37.5	27.0	40.0
72-73	33.12847529829376	35.0	34.0	37.0	26.0	39.0
74-75	32.77990395842136	35.0	34.0	37.0	26.0	39.0
76-77	31.336762948021143	34.0	31.5	35.0	24.0	37.0
78-79	32.13303444644846	35.0	33.0	36.0	26.0	37.0
80-81	32.10035457797687	35.0	33.0	35.0	26.0	37.0
82-83	31.941302308359795	35.0	33.0	35.0	25.5	36.5
84-85	31.75685986036465	35.0	33.0	35.0	25.0	36.0
86-87	31.575076273954238	35.0	33.0	35.0	25.0	36.0
88-89	31.416596626884072	35.0	33.0	35.0	24.0	36.0
90-91	31.277203640857195	35.0	33.0	35.0	24.0	35.0
92-93	31.10756154540824	35.0	33.0	35.0	23.0	35.0
94-95	30.99999040896671	35.0	33.0	35.0	20.5	35.0
96-97	30.8786943145869	35.0	33.0	35.0	19.5	35.0
98-99	30.727499309697997	35.0	33.0	35.0	17.5	35.0
100	30.37216957249736	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.43765606585530037
1101	2	0.366217442169237
1101	3	0.419940390148156
1101	4	0.28987365072273263
1101	5	0.3152769447864401
1101	6	0.3140511773787509
1101	7	0.30361593650735585
1101	8	0.3385723388719555
1101	9	0.3699335353417723
1101	10-11	0.4062390761407215
1101	12-13	0.44339370644998866
1101	14-15	0.5672921205808876
1101	16-17	0.5333351909312398
1101	18-19	0.524486840293541
1101	20-21	0.5290500290983147
1101	22-23	0.5029023707284423
1101	24-25	0.5774811078352045
1101	26-27	0.6358851619022445
1101	28-29	0.6376227961127938
1101	30-31	0.6497030584505978
1101	32-33	0.651833526640651
1101	34-35	0.602922259633587
1101	36-37	0.6687511700152129
1101	38-39	0.6730261451759461
1101	40-41	0.7356321242276351
1101	42-43	0.8028370095944268
1101	44-45	0.9349081766706391
1101	46-47	0.9950300854215541
1101	48-49	0.964938313704927
1101	50-51	0.9764708577058201
1101	52-53	0.935094581648606
1101	54-55	1.0144768572817995
1101	56-57	1.0186561297556196
1101	58-59	1.1050708468516746
1101	60-61	1.1465180461649993
1101	62-63	1.1204635284033841
1101	64-65	1.020498148437575
1101	66-67	0.995658379288848
1101	68-69	1.0580809747057884
1101	70-71	1.0285392238098865
1101	72-73	1.011121987214544
1101	74-75	1.0606630135120483
1101	76-77	1.065437699380169
1101	78-79	1.0591077917874756
1101	80-81	1.1106853199152198
1101	82-83	1.0615673525903269
1101	84-85	1.0691932721254034
1101	86-87	1.0897813416179787
1101	88-89	1.0906533727336019
1101	90-91	1.0394563860138355
1101	92-93	1.1008932223818846
1101	94-95	1.076699680669293
1101	96-97	1.1536420707574955
1101	98-99	1.2097456066169023
1101	100	1.19983272801975
1102	1	0.4047461349838315
1102	2	0.31330897139914526
1102	3	0.3670946875740384
1102	4	0.25379971786961875
1102	5	0.2711853851153023
1102	6	0.27356596036646863
1102	7	0.3093790267068641
1102	8	0.32479727907379896
1102	9	0.33229309150328135
1102	10-11	0.3333545596538343
1102	12-13	0.349473493178202
1102	14-15	0.4160882508273147
1102	16-17	0.49847958068024667
1102	18-19	0.46223425945658647
1102	20-21	0.441175632299327
1102	22-23	0.5179244433127295
1102	24-25	0.46999932744899553
1102	26-27	0.5203426520741701
1102	28-29	0.5780010916788001
1102	30-31	0.566355245329575
1102	32-33	0.6172896022383441
1102	34-35	0.6653912964596671
1102	36-37	0.722403026350932
1102	38-39	0.696652040262542
1102	40-41	0.815333455371352
1102	42-43	0.8065154144525266
1102	44-45	0.8462618388177319
1102	46-47	0.9418926319622898
1102	48-49	0.9550143386516439
1102	50-51	0.9419455213100463
1102	52-53	1.03916600161876
1102	54-55	1.119852939797461
1102	56-57	1.1473353764618395
1102	58-59	1.0501399201533488
1102	60-61	1.1009685402422988
1102	62-63	1.0810514320291489
1102	64-65	1.1270528183240103
1102	66-67	1.1505172168487974
1102	68-69	1.0812256020580122
1102	70-71	1.0824162913552016
1102	72-73	1.0754412041691737
1102	74-75	1.0319919563043598
1102	76-77	1.0147642343521568
1102	78-79	1.0410026693369687
1102	80-81	0.9936946966216453
1102	82-83	1.0052460968815886
1102	84-85	1.009710843044708
1102	86-87	1.0629728565431975
1102	88-89	1.1194611460762935
1102	90-91	1.1196792589060856
1102	92-93	1.156741022706825
1102	94-95	1.1749153397986518
1102	96-97	1.191413842903021
1102	98-99	1.2014152163846035
1102	100	1.26993564361592
1103	1	0.32042127471706294
1103	2	0.27362751358425896
1103	3	0.30053585702053454
1103	4	0.20844386738293963
1103	5	0.27599323997850433
1103	6	0.23965177674357818
1103	7	0.2775524053663858
1103	8	0.2714635073931504
1103	9	0.30497169164112137
1103	10-11	0.3212132744328642
1103	12-13	0.3254777528984576
1103	14-15	0.4485694530108759
1103	16-17	0.38811548645749383
1103	18-19	0.3997928867398812
1103	20-21	0.4442129582416996
1103	22-23	0.470428341997831
1103	24-25	0.46353413884137495
1103	26-27	0.4823601242240798
1103	28-29	0.5018512418461683
1103	30-31	0.5022176154975782
1103	32-33	0.5595858218535525
1103	34-35	0.5538259568494084
1103	36-37	0.640798993234867
1103	38-39	0.6223850458510682
1103	40-41	0.7540623898091638
1103	42-43	0.6979600374878601
1103	44-45	0.6913988096586792
1103	46-47	0.7231820773740836
1103	48-49	0.694314053418509
1103	50-51	0.7791633402354492
1103	52-53	0.8525692994606615
1103	54-55	0.8901407696053738
1103	56-57	0.8879281489966004
1103	58-59	0.882741961862898
1103	60-61	0.9287045926119717
1103	62-63	0.913161068039166
1103	64-65	0.862162473979474
1103	66-67	0.8143376663124968
1103	68-69	0.8198441411292521
1103	70-71	0.7647809372541019
1103	72-73	0.8052828739196443
1103	74-75	0.8398016483506723
1103	76-77	0.7571220564662724
1103	78-79	0.8114503689557253
1103	80-81	0.7580308256219155
1103	82-83	0.7616221125476947
1103	84-85	0.8056875296494965
1103	86-87	0.7838147079137912
1103	88-89	0.8849772044892852
1103	90-91	0.8863356217379241
1103	92-93	0.8286540586081337
1103	94-95	0.8131278195735376
1103	96-97	0.9517879068544204
1103	98-99	0.9401563056055231
1103	100	0.9838886931434203
1104	1	0.32027946722485723
1104	2	0.23521793296844606
1104	3	0.26651141554684443
1104	4	0.21511400014855298
1104	5	0.2892236091353766
1104	6	0.26977890551553685
1104	7	0.28035284233595803
1104	8	0.2605246519708757
1104	9	0.26668873118919834
1104	10-11	0.30153792317516803
1104	12-13	0.33220872361929565
1104	14-15	0.4421392642739477
1104	16-17	0.4430178161778997
1104	18-19	0.3483924870314681
1104	20-21	0.41909562874368333
1104	22-23	0.41395627090364684
1104	24-25	0.431056510012354
1104	26-27	0.4529775882018541
1104	28-29	0.521291839556369
1104	30-31	0.4689588044735231
1104	32-33	0.4897065703717729
1104	34-35	0.4668578979479534
1104	36-37	0.49965300668630874
1104	38-39	0.5153255107755754
1104	40-41	0.5211331930549434
1104	42-43	0.5469795762713545
1104	44-45	0.4867285033123423
1104	46-47	0.47583180325560903
1104	48-49	0.45295054138646407
1104	50-51	0.4870472438854634
1104	52-53	0.511053994908238
1104	54-55	0.4777832758919658
1104	56-57	0.46260738415249847
1104	58-59	0.5240377336831088
1104	60-61	0.5618198902778957
1104	62-63	0.5977651316324355
1104	64-65	0.5523656386720006
1104	66-67	0.5612826692343376
1104	68-69	0.5435502664446901
1104	70-71	0.5150083827616285
1104	72-73	0.5385665992534783
1104	74-75	0.5976599088567269
1104	76-77	0.5026737155824108
1104	78-79	0.6292370882812506
1104	80-81	0.6173016337292125
1104	82-83	0.6280211453965627
1104	84-85	0.585849051515126
1104	86-87	0.5996181611143498
1104	88-89	0.6521192758982473
1104	90-91	0.5941774007425558
1104	92-93	0.6347978293038246
1104	94-95	0.6672439479922261
1104	96-97	0.6947422866021604
1104	98-99	0.6548385577409412
1104	100	0.7008473012324643
1105	1	0.17761769082079581
1105	2	0.09210734350408956
1105	3	0.16846263454463184
1105	4	0.14782597978042844
1105	5	0.1661885986936511
1105	6	0.1489556738154434
1105	7	0.15939798622293466
1105	8	0.15902695632082953
1105	9	0.16981486811725688
1105	10-11	0.18939379154303282
1105	12-13	0.21311248014382755
1105	14-15	0.22570943015691114
1105	16-17	0.21704112577349832
1105	18-19	0.22293106340684687
1105	20-21	0.2893500932349795
1105	22-23	0.28744943588989713
1105	24-25	0.29270880327203486
1105	26-27	0.27126338102582537
1105	28-29	0.30538799339576883
1105	30-31	0.31236832143353865
1105	32-33	0.29554259015075957
1105	34-35	0.3688670288753144
1105	36-37	0.3587383426827131
1105	38-39	0.34331134968304156
1105	40-41	0.2972135537223508
1105	42-43	0.31525715245586383
1105	44-45	0.40523741085256404
1105	46-47	0.3636426251058609
1105	48-49	0.378720321869487
1105	50-51	0.371275356076076
1105	52-53	0.41076327526419476
1105	54-55	0.4159487402050317
1105	56-57	0.4311466625013125
1105	58-59	0.38049950108841557
1105	60-61	0.3883913159870005
1105	62-63	0.3326801021562247
1105	64-65	0.4105264401520188
1105	66-67	0.4003546002447962
1105	68-69	0.3708571912351033
1105	70-71	0.3411668234736993
1105	72-73	0.3499213974369937
1105	74-75	0.3616244874951491
1105	76-77	0.3375517244103321
1105	78-79	0.3804145546879383
1105	80-81	0.3873626952241196
1105	82-83	0.3997642628405451
1105	84-85	0.37183515546414014
1105	86-87	0.4032401260986376
1105	88-89	0.4967504182097393
1105	90-91	0.5039523105177004
1105	92-93	0.49692628341209044
1105	94-95	0.5163696360369201
1105	96-97	0.5634084615007602
1105	98-99	0.5692849478153086
1105	100	0.5992103884673377
1106	1	0.17378071057736832
1106	2	0.10705982109287504
1106	3	0.1809388392238631
1106	4	0.15458804216827104
1106	5	0.1714496917237298
1106	6	0.1196745386190301
1106	7	0.1624038725492909
1106	8	0.17232153483783463
1106	9	0.18581131819167496
1106	10-11	0.20580539868849712
1106	12-13	0.1966601053878776
1106	14-15	0.29828415940441744
1106	16-17	0.2943395856204418
1106	18-19	0.2445106535766115
1106	20-21	0.28781237698144224
1106	22-23	0.3268561684670388
1106	24-25	0.27383257110573567
1106	26-27	0.3197179157584671
1106	28-29	0.30079828662039887
1106	30-31	0.3043974616244185
1106	32-33	0.30818554483511207
1106	34-35	0.31048483776611135
1106	36-37	0.30299101121889294
1106	38-39	0.2505061871828076
1106	40-41	0.34982192538650025
1106	42-43	0.3674090146072402
1106	44-45	0.3157829657940425
1106	46-47	0.36169750079510976
1106	48-49	0.41964474023045994
1106	50-51	0.3831709695494183
1106	52-53	0.410339028941074
1106	54-55	0.4486877631664825
1106	56-57	0.4702885640020895
1106	58-59	0.4906479556231531
1106	60-61	0.450718754221505
1106	62-63	0.3923788945771989
1106	64-65	0.4436102454928914
1106	66-67	0.443602991715224
1106	68-69	0.4496087617506461
1106	70-71	0.5218293787768005
1106	72-73	0.49356359963849883
1106	74-75	0.4858273381575202
1106	76-77	0.3527438365965594
1106	78-79	0.4983721388280955
1106	80-81	0.5292410951648634
1106	82-83	0.5311891212870243
1106	84-85	0.4983289362713492
1106	86-87	0.5744986926577837
1106	88-89	0.587060130209597
1106	90-91	0.586774330778205
1106	92-93	0.5828755912155898
1106	94-95	0.6085115755517236
1106	96-97	0.6362665658482918
1106	98-99	0.6740000277861071
1106	100	0.6989420971395077
1107	1	0.13031010499577178
1107	2	0.0689720768005131
1107	3	0.1008575450281981
1107	4	0.06131227546148921
1107	5	0.12417240268036522
1107	6	0.08514176646956173
1107	7	0.0456527158279556
1107	8	0.10079917392880589
1107	9	0.07245594877432637
1107	10-11	0.12550057679589344
1107	12-13	0.1542718678330175
1107	14-15	0.18611802188817705
1107	16-17	0.16480234993370146
1107	18-19	0.111405535075356
1107	20-21	0.1841507000086935
1107	22-23	0.1520535333428299
1107	24-25	0.16009759987595373
1107	26-27	0.14016911192284454
1107	28-29	0.1572374419930327
1107	30-31	0.2562602095841555
1107	32-33	0.2417534267140624
1107	34-35	0.22995305000857513
1107	36-37	0.28855994428847254
1107	38-39	0.28203556756786696
1107	40-41	0.3298723415591951
1107	42-43	0.40975379558985736
1107	44-45	0.40754453525271117
1107	46-47	0.4288485936366797
1107	48-49	0.3815479912124218
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2312	74-75	5.7476846521353764E-5
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2312	80-81	-0.19593401802000443
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2313	1	-0.21585080036932425
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2314	1	-0.2985384966122666
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2314	4	-0.20804988098792876
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2314	96-97	-0.6496312180922352
2314	98-99	-0.7253756363472483
2314	100	-0.8002346962124989
2315	1	-0.4547707196380415
2315	2	-0.45442369583847864
2315	3	-0.43180675961693993
2315	4	-0.28413265020238754
2315	5	-0.3133014048203009
2315	6	-0.2782329184978991
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2315	10-11	-0.35651055013910593
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2315	24-25	-0.5281180875886449
2315	26-27	-0.5137125442234733
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2315	100	-0.7867635722478958
2316	1	-0.5446125165044151
2316	2	-0.44436237858238314
2316	3	-0.42256370671312027
2316	4	-0.35247170485911283
2316	5	-0.39053533713167354
2316	6	-0.3423330579266164
2316	7	-0.3515539019345155
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2316	9	-0.5213935334887125
2316	10-11	-0.590650238314872
2316	12-13	-0.5661893343909696
2316	14-15	-0.7607449605318166
2316	16-17	-0.7723569731331921
2316	18-19	-0.7656715595397898
2316	20-21	-0.831929327876999
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2316	26-27	-0.6708811142038726
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2316	30-31	-0.8178021126047099
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2316	36-37	-0.7249942458002181
2316	38-39	-0.7441918044326385
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2316	52-53	-0.742747934223388
2316	54-55	-0.8406582937124938
2316	56-57	-0.8344055233407772
2316	58-59	-0.8283648415938742
2316	60-61	-0.6851124354143892
2316	62-63	-0.6593482098249481
2316	64-65	-0.7346104724238813
2316	66-67	-0.6345670499246552
2316	68-69	-0.5610471280668037
2316	70-71	-0.5859915498649997
2316	72-73	-0.5362636557278151
2316	74-75	-0.6417274191877453
2316	76-77	-0.6273091112147213
2316	78-79	-0.7164780424827804
2316	80-81	-0.646819294855181
2316	82-83	-0.6853389685709281
2316	84-85	-0.7068413456553664
2316	86-87	-0.7436218881276275
2316	88-89	-0.7043630245514443
2316	90-91	-0.7133348785024616
2316	92-93	-0.7175498299508192
2316	94-95	-0.6598390690029561
2316	96-97	-0.6979521840788472
2316	98-99	-0.7196945719925374
2316	100	-0.7833214002276705
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20.0
3	47.0
4	81.0
5	142.0
6	365.0
7	893.0
8	2035.0
9	3523.0
10	6109.0
11	11110.0
12	17666.0
13	24877.0
14	28973.0
15	37544.0
16	50130.0
17	66160.0
18	66455.0
19	41902.0
20	29668.0
21	26047.0
22	27417.0
23	30948.0
24	35234.0
25	40347.0
26	49175.0
27	62105.0
28	78114.0
29	98760.0
30	124025.0
31	155734.0
32	199640.0
33	262279.0
34	376753.0
35	616083.0
36	1167679.0
37	2099689.0
38	1783421.0
39	302706.0
40	212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.76729225443295	18.7982107170206	13.707517401415537	23.726979627130913
2	15.328691214045303	22.783402771508104	36.03386065939644	25.854045355050154
3	19.492714967961916	27.36843380836527	29.54841460646841	23.590436617204404
4	13.83335268490426	18.665486220840126	34.78579073042925	32.71537036382636
5	13.738839696983923	37.56077055371054	32.91388968393507	15.786500065370465
6	31.367158888591064	34.59423871678032	17.863690720473375	16.174911674155247
7	25.789745872953134	32.62430357740494	22.186104410007587	19.399846139634338
8	27.956385533289215	31.34589455819915	21.217725541981718	19.47999436652992
9	28.2644343789074	14.53935024282982	19.53577379699417	37.660441581268614
10-11	25.28335534014693	25.92314723473229	25.803593637151458	22.989903787969325
12-13	27.36775094113752	23.80953462649708	26.263827438339803	22.558886994025595
14-15	25.820011211455572	23.39552132385029	24.914096827905166	25.87037063678897
16-17	23.499582567546224	26.39420578135903	27.147433003368924	22.958778647725815
18-19	24.84304147819024	25.37810755788567	28.444663776232108	21.334187187691978
20-21	24.805285367024407	25.159305662362293	27.541566181394767	22.493842789218537
22-23	23.354897520523377	25.970440482508916	25.637648078548068	25.03701391841964
24-25	23.591023438373533	26.784360805016245	25.113805957435208	24.510809799175014
26-27	24.43506061809916	25.289517866905197	25.748923906958527	24.52649760803712
28-29	23.831772693266455	24.56642576825492	28.009330774923548	23.592470763555077
30-31	25.2696071456313	24.8258099982578	26.876330509615194	23.0282523464957
32-33	22.542804472633186	25.80871892773412	27.375579215841057	24.27289738379164
34-35	22.57339478167789	26.37755395970353	27.62455269284727	23.424498565771305
36-37	24.40499973623693	26.173007370978844	26.230113968362062	23.191878924422163
38-39	24.82958852121	24.90528488866778	26.15809258279085	24.107034007331364
40-41	23.7354972924325	26.000257824510097	26.27132060730229	23.99292427575511
42-43	25.728213263643422	25.13607787413299	25.93114457360352	23.204564288620066
44-45	25.421492538594247	24.912850674838587	26.318193881582925	23.347462904984237
46-47	24.112099547905903	25.333549304329615	27.735818589915944	22.81853255784854
48-49	24.91243605792556	26.031836052865025	26.821506356616265	22.234221532593146
50-51	23.40988465055562	26.78379469156814	25.730989022860147	24.075331635016095
52-53	23.089505335361043	26.963785172050407	24.2965148197462	25.650194672842353
54-55	24.05365136081575	26.358648012969827	24.107942630353005	25.47975799586142
56-57	24.741578538091353	24.895267208179764	24.608711145265552	25.754443108463327
58-59	24.09759598768292	24.83812180904685	26.502346754092336	24.561935449177895
60-61	24.46749452044212	25.47938873329183	25.61063430584441	24.442482440421642
62-63	22.31513825648435	26.019352399241647	26.337212193356898	25.328297150917113
64-65	22.285657565131604	26.06472588340572	26.12700223454035	25.522614316922326
66-67	24.10868855764991	25.88086224551221	25.102113135523812	24.90833606131407
68-69	24.49970683827796	24.85150090472509	25.134781196357274	25.514011060639675
70-71	23.247162783994504	25.62053787373709	25.450769953204507	25.681529389063908
72-73	24.5845927717202	25.023348367614517	25.4132397627638	24.978819097901482
74-75	24.742431456140732	24.610768940014204	25.656723985452444	24.99007561839262
76-77	23.434137780857593	25.633093382638467	26.542023215832327	24.39074562067161
78-79	23.72654710367331	25.505283464042254	26.295727090265885	24.472442342018542
80-81	22.66670351642616	25.78179541114488	25.89778381508084	25.65371725734812
82-83	22.641354898992372	26.39526897928257	24.437313887154346	26.526062234570713
84-85	23.008839841407205	25.97224846961488	24.31003395667692	26.708877732300994
86-87	23.202877024722113	24.76252740165518	25.01327766798151	27.021317905641197
88-89	23.265168592499112	24.381180739046417	25.930449185629218	26.423201482825252
90-91	23.814529082698385	24.93109561505619	25.276829045444586	25.97754625680084
92-93	22.263679936072727	25.559923647191262	25.617874144316804	26.558522272419204
94-95	21.679980630868563	25.86209387908061	26.118563239021558	26.339362251029268
96-97	23.15021452403025	25.37978034980579	25.09811265799047	26.371892468173485
98-99	23.300241744518093	24.869808032944697	24.998372048296407	26.831578174240807
100	23.099645646540782	25.567234969066792	25.34516303552911	25.98795634886331
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	767.0
1	509.5
2	313.5
3	390.5
4	371.0
5	433.5
6	684.5
7	1424.5
8	2298.5
9	2423.0
10	1978.5
11	1805.0
12	1976.0
13	2191.0
14	2556.0
15	2984.0
16	3359.0
17	3908.0
18	4582.0
19	5293.5
20	6355.5
21	7708.0
22	9470.0
23	11562.0
24	13912.5
25	17251.0
26	22382.0
27	28509.5
28	34492.5
29	42943.5
30	52522.5
31	62350.0
32	72619.0
33	81729.0
34	92790.5
35	102143.0
36	109148.0
37	117903.5
38	124941.5
39	132432.5
40	138141.5
41	142968.5
42	149546.0
43	163726.5
44	186142.5
45	206661.0
46	236887.5
47	277276.5
48	385878.0
49	518513.5
50	680536.5
51	651476.0
52	425560.0
53	331800.5
54	297180.0
55	265009.0
56	243919.0
57	222819.0
58	212623.0
59	196971.0
60	164985.0
61	132915.5
62	108152.5
63	85091.5
64	62633.0
65	50401.0
66	39841.5
67	29914.5
68	23084.5
69	18803.5
70	16215.5
71	13697.0
72	11935.5
73	12894.5
74	10351.5
75	7461.5
76	5761.0
77	4040.5
78	2903.0
79	1758.5
80	1183.5
81	950.5
82	781.0
83	618.5
84	429.5
85	245.5
86	147.5
87	80.5
88	52.0
89	26.0
90	14.0
91	12.5
92	9.5
93	4.0
94	2.5
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.05745786129043819
3	0.02440665577327201
4	0.0064108485691945095
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	8.833846453614481E-5
12-13	1.703670387482793E-4
14-15	3.785934194406207E-4
16-17	3.975230904126517E-4
18-19	0.0
20-21	0.0028836198780727274
22-23	0.004536811142963438
24-25	6.814681549931172E-4
26-27	4.0383298073666204E-4
28-29	0.004997433136616192
30-31	0.002240011065023672
32-33	0.0012682879551260792
34-35	0.0015459231293825342
36-37	0.0036597363879259997
38-39	0.0038616528782943306
40-41	9.275538776295207E-4
42-43	0.002580745142520231
44-45	3.154945162005172E-4
46-47	0.0020759539165994033
48-49	1.892967097203103E-5
50-51	0.0018424879746110204
52-53	0.0065370463756747165
54-55	0.013414826828845991
56-57	0.023769356850546968
58-59	0.02602198769621866
60-61	0.023927104108647224
62-63	0.0076475870727005365
64-65	0.006492877143406644
66-67	1.7667692907228963E-4
68-69	0.0014386549938743586
70-71	1.8929670972031034E-4
72-73	8.392154130933758E-4
74-75	0.005054222149532286
76-77	9.969626711936344E-4
78-79	0.0012619780648020688
80-81	0.0
82-83	0.001413415432578317
84-85	6.309890324010344E-6
86-87	6.120593614290033E-4
88-89	6.940879356411379E-5
90-91	5.552703485129103E-4
92-93	8.013560711493137E-4
94-95	0.0015080637874384723
96-97	1.7667692907228963E-4
98-99	0.0
100	7.445670582332207E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7924068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.490107446910706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.7634894879955	25.708175109527698
2	12.67065573992382	8.486832443057292
3	3.8617847204646796	3.879947556756004
4	1.8106891169228205	2.4256069231478845
5	1.0326722182094166	1.7292151772636493
6	0.6591538802058324	1.3245080563284861
7	0.4710096878059411	1.1041915537209785
8	0.35932736328841847	0.96271296041154
9	0.2536136092327038	0.7644192320881866
>10	1.7087491514577637	11.074510913667163
>50	0.20378333947814734	4.778760807347422
>100	0.16921115177603174	11.447995175733354
>500	0.021516352010219536	4.9630216619992185
>1k	0.012295012481808816	7.657489108518827
>5k	7.968989571223266E-4	1.9609623044230036
>10k+	0.001252269789763656	11.731651016009407
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	114228	1.4415323038621073	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	103073	1.3007586507334363	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	50440	0.6365417358861635	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	49898	0.6297018147749363	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	44786	0.5651894961022546	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	43903	0.5540462297900524	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	39775	0.5019517752750229	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	38014	0.47972834155385846	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	36826	0.4647360421440099	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	36254	0.4575175276133421	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	33412	0.4216521110116673	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	28873	0.36437092665030135	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22334	0.28185018099289405	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	21423	0.2703535608225472	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	19099	0.24102519059654715	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	17284	0.21812028872038955	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	16849	0.21263068413850056	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	15968	0.20151265738759436	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15472	0.1952532461861761	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	15331	0.19347385711480516	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	14760	0.18626796236478535	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	14237	0.17966781708587054	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	14168	0.1787970522211571	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13369	0.16871384748338858	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	12978	0.1637795132500125	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	12916	0.16299708684983522	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	12597	0.15897137682311663	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	12478	0.15746962292600217	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	10609	0.13388325289485148	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	10584	0.13356775837865098	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	10488	0.13235625943644097	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	10411	0.1313845363265434	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	10284	0.12978182418424475	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	9919	0.1251756042477172	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	9758	0.12314381956338588	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	9736	0.12286618438912941	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	9420	0.11887833370435488	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	9326	0.11769207432344095	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	8558	0.10800008278576106	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	8261	0.1042520079332989	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003003507794228924	0.0	0.0	2.5239561296041377E-5	0.0
2	0.0047197979623597374	1.2619780648020689E-5	0.0	2.5239561296041377E-5	0.0
3	0.005060532039856296	1.2619780648020689E-5	0.0	3.7859341944062066E-5	0.0
4	0.005641041949665248	5.0479122592082755E-5	0.0	5.0479122592082755E-5	0.0
5	0.024633811824936385	6.309890324010344E-5	0.0	2.5239561296041375E-4	0.0
6	0.05293997981844679	6.309890324010344E-5	0.0	5.552703485129103E-4	0.0
7	0.05965370312319379	6.309890324010344E-5	0.0	6.057494711049931E-4	0.0
8	0.06901758036402514	7.571868388812413E-5	0.0	7.319472775851999E-4	0.0
9	0.07530223112673945	1.1357802583218619E-4	0.0	8.455253034173861E-4	0.0
10-11	0.13925296956058428	1.1357802583218619E-4	0.0	0.001672120935862741	1.8929670972031033E-5
12-13	0.23941616856392448	1.1357802583218619E-4	0.0	0.0038237935363502687	4.4169232268072414E-5
14-15	0.35399115706730433	1.2619780648020687E-4	0.0	0.006322510104658365	5.0479122592082755E-5
16-17	0.40641372587918223	1.2619780648020687E-4	0.0	0.007483529924276268	5.0479122592082755E-5
18-19	0.43045440801366164	1.3881758712822756E-4	0.0	0.008038800272789179	5.0479122592082755E-5
20-21	0.5169630043558435	1.6405714842426895E-4	0.0	0.010436558595913109	5.6789012916093096E-5
22-23	0.5409658271483788	1.703670387482793E-4	0.0	0.012581921306076627	6.309890324010344E-5
24-25	0.6510671034120353	1.8929670972031034E-4	0.0	0.023075268914905828	6.309890324010344E-5
26-27	0.881497988154569	1.8929670972031034E-4	0.0	0.057142366774237674	6.309890324010344E-5
28-29	0.935100506457037	1.8929670972031034E-4	0.0	0.08528447761932381	6.309890324010344E-5
30-31	0.9526924806803778	2.2715605166437238E-4	0.0	0.14784073029156236	6.309890324010344E-5
32-33	0.9684482768194316	2.2715605166437238E-4	0.0	0.24196536425482468	6.309890324010344E-5
34-35	0.9805821959125035	2.3346594198838272E-4	0.0	0.3603325968429347	6.309890324010344E-5
36-37	1.0004772801041082	2.460857226364034E-4	0.0	0.5384608006897467	6.309890324010344E-5
38-39	1.020618450018349	2.7763517425645517E-4	0.0	0.8050158075372398	6.309890324010344E-5
40-41	1.0388792726160352	3.0287473555249653E-4	0.0	1.186372958939777	6.309890324010344E-5
42-43	1.0749276760371063	3.2180440652452755E-4	0.0	1.4671567684679132	6.309890324010344E-5
44-45	1.1165224730529824	3.659736387926E-4	0.0	1.7529317012423418	6.309890324010344E-5
46-47	1.1332184428503136	3.9121320008864136E-4	0.0	2.0863652356340205	6.940879356411378E-5
48-49	1.144254441027008	3.9121320008864136E-4	0.0	2.388343966760507	7.571868388812413E-5
50-51	1.1636131845410715	4.0383298073666204E-4	0.0	2.7285858728117933	7.571868388812413E-5
52-53	1.1696580594714736	4.0383298073666204E-4	0.0	3.1291818798122377	8.833846453614481E-5
54-55	1.1898307788373346	4.0383298073666204E-4	0.0	3.4851669622219292	8.833846453614481E-5
56-57	1.231002813201502	4.0383298073666204E-4	0.0	3.858762948525934	8.833846453614481E-5
58-59	1.2440895257334996	4.0383298073666204E-4	0.0	4.249244201336991	8.833846453614481E-5
60-61	1.25294861174841	4.164527613846827E-4	0.0	4.610668409205978	8.833846453614481E-5
62-63	1.2604573812339823	4.290725420327034E-4	0.0	4.984807046077847	1.0095824518416551E-4
64-65	1.2666852429837805	5.174110065688482E-4	0.0	5.3877566421691485	1.0095824518416551E-4
66-67	1.27491333996629	5.300307872168689E-4	0.0	5.80527577501859	1.0095824518416551E-4
68-69	1.283923863348977	5.363406775408793E-4	0.0	6.302608710576436	1.0095824518416551E-4
70-71	1.295704428583904	5.426505678648896E-4	0.0	6.902105080370335	1.0095824518416551E-4
72-73	1.3253104339841606	5.426505678648896E-4	0.0	7.401248954451173	1.0095824518416551E-4
74-75	1.3536733909905871	5.426505678648896E-4	0.0	7.878730975049685	1.0095824518416551E-4
76-77	1.3625955759087378	5.615802388369206E-4	0.0	8.399132869632112	1.1357802583218619E-4
78-79	1.3691957211876526	5.805099098089517E-4	0.0	8.912076473851563	1.1357802583218619E-4
80-81	1.3819038403002093	5.805099098089517E-4	0.0	9.490945812176271	1.1357802583218619E-4
82-83	1.3867687657400214	5.86819800132962E-4	0.0	10.158046346901617	1.1357802583218619E-4
84-85	1.4042093025955862	5.931296904569724E-4	0.0	10.773815923841138	1.1357802583218619E-4
86-87	1.4336070816151503	6.183692517530137E-4	0.0	11.388323522715858	1.1357802583218619E-4
88	1.4409896532942423	6.562285936970758E-4	0.0	11.883012614227944	1.1357802583218619E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATC	2285	0.0	24.274387	3
TCTCGCG	1050	0.0	23.720507	8
GATCTAC	3035	0.0	21.540752	1
CGATCTC	2370	0.0	20.81936	5
TCTACAC	4385	0.0	20.796232	3
AGTACTT	27075	0.0	19.526613	12-13
CTCGCGC	1305	0.0	19.445568	9
AGTACTC	2610	0.0	18.72489	5
ATCTACA	4110	0.0	17.852028	2
CCGATCT	3135	0.0	17.83859	4
TACACTC	4105	0.0	17.51478	5
GAGTACT	26190	0.0	17.225769	12-13
GTATAAG	2555	0.0	16.935642	1
TACTTTT	31560	0.0	16.916435	14-15
GTATTAG	3365	0.0	16.632849	1
CGTTAAC	800	0.0	16.46163	1
CGCGCCA	1620	0.0	16.258398	1
TAGTACT	2325	0.0	16.170303	4
CTACACT	6205	0.0	15.601866	4
GTACTTT	29285	0.0	15.438225	14-15
>>END_MODULE