FastQCFastQC Report
Fri 27 May 2016
ERR765529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12362198
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA883080.7143389872901242No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT857360.6935336256545963No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG365980.2960476769584179No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC255630.20678361566446357No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC254490.20586144955775662No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG220430.17830971482579394No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG218410.1766757011981203No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT184730.1494313551683932No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC165550.1339163148818681No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150960.12211420655129453No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG146000.11810197506948199No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT133230.10777209683908963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG39400.020.980318
GTATAAG62850.020.7033311
GTACTAG58450.019.770441
TAGTACT61900.019.5780074
CTGTGCG62100.018.8322949
TGATCGC44450.018.7023859
CGTTAAC16350.018.1004141
CATGGGG278950.017.8155274
GTATAAT65200.017.5075111
TAGGCAT81200.017.237045
ATAAGGT56500.017.2096423
GGCGTCG38350.017.1458138
GTCCTAC50100.017.064731
ACGTTAA14050.017.0486248
TACGGGC38450.016.9807874
CTCGTCG36500.016.5993989
CTAGGCA89000.016.5193794
CGGGCGT39050.016.4775716
ATACGGG40800.016.4636363
CTAGTAC81850.016.1837943
Produced by FastQC (version 0.11.5)