##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765529_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12362198
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99103290531344	33.0	33.0	33.0	27.0	33.0
2	31.911032083453122	33.0	33.0	33.0	27.0	33.0
3	31.92115803354711	33.0	33.0	33.0	27.0	33.0
4	35.68259471333496	37.0	37.0	37.0	33.0	37.0
5	35.74851324982823	37.0	37.0	37.0	33.0	37.0
6	35.780386546146566	37.0	37.0	37.0	33.0	37.0
7	35.602212486808575	37.0	37.0	37.0	33.0	37.0
8	35.58200143696129	37.0	37.0	37.0	33.0	37.0
9	35.448818082350726	37.0	37.0	37.0	33.0	37.0
10-11	35.42081634673704	37.0	37.0	37.0	33.0	37.0
12-13	35.37396683017049	37.0	37.0	37.0	33.0	37.0
14-15	37.04796299169452	40.0	37.0	40.0	33.0	40.0
16-17	37.08265463795354	40.0	37.0	40.0	33.0	40.0
18-19	37.02986616943039	40.0	37.0	40.0	33.0	40.0
20-21	36.735062486460734	40.0	37.0	40.0	33.0	40.0
22-23	36.79768423867665	40.0	37.0	40.0	33.0	40.0
24-25	36.69367360885176	40.0	37.0	40.0	33.0	40.0
26-27	36.66448138915102	40.0	37.0	40.0	33.0	40.0
28-29	36.606166476220494	40.0	37.0	40.0	33.0	40.0
30-31	36.73186835383157	40.0	37.0	40.0	33.0	40.0
32-33	36.499883192293154	37.0	37.0	40.0	33.0	40.0
34-35	36.34469295023426	37.0	37.0	40.0	33.0	40.0
36-37	36.477291093379996	37.0	37.0	40.0	33.0	40.0
38-39	36.41011646957928	37.0	37.0	40.0	33.0	40.0
40-41	36.462632777763304	37.0	37.0	40.0	33.0	40.0
42-43	36.385636720913226	37.0	37.0	40.0	33.0	40.0
44-45	36.299228786013614	37.0	37.0	40.0	33.0	40.0
46-47	36.11310953764047	37.0	37.0	40.0	30.0	40.0
48-49	35.91704189659477	37.0	37.0	40.0	27.0	40.0
50-51	35.742234390680366	37.0	37.0	40.0	27.0	40.0
52-53	35.619509370421014	37.0	37.0	40.0	27.0	40.0
54-55	35.496967974465385	37.0	33.0	40.0	27.0	40.0
56-57	35.34938131552334	37.0	33.0	40.0	27.0	40.0
58-59	35.17738022801447	37.0	33.0	40.0	27.0	40.0
60-61	34.9551473370674	37.0	33.0	40.0	27.0	40.0
62-63	34.72035365393759	37.0	33.0	38.5	27.0	40.0
64-65	34.54608626232972	37.0	33.0	37.0	27.0	40.0
66-67	34.30039888537621	37.0	33.0	37.0	27.0	40.0
68-69	34.08187649963219	37.0	33.0	37.0	27.0	40.0
70-71	33.765862065952994	37.0	33.0	37.0	27.0	40.0
72-73	33.53411193543413	37.0	33.0	37.0	27.0	38.5
74-75	33.32942442759774	37.0	33.0	37.0	27.0	37.0
76-77	33.044697552975606	37.0	33.0	37.0	27.0	37.0
78-79	32.93442472770619	37.0	33.0	37.0	27.0	37.0
80-81	32.787361438475585	37.0	33.0	37.0	27.0	37.0
82-83	32.640205568621376	35.0	33.0	37.0	27.0	37.0
84-85	32.476299805261164	33.0	33.0	37.0	22.0	37.0
86-87	32.30178553199035	33.0	33.0	37.0	22.0	37.0
88-89	32.17031870060647	33.0	33.0	37.0	22.0	37.0
90-91	32.052675220053914	33.0	33.0	37.0	22.0	37.0
92-93	31.913482092747586	33.0	33.0	37.0	22.0	37.0
94-95	31.798020788859716	33.0	33.0	37.0	22.0	37.0
96-97	31.68744126246805	33.0	33.0	37.0	22.0	37.0
98-99	31.578813168984997	33.0	33.0	37.0	22.0	37.0
100	31.337234365603916	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	15.0
7	255.0
8	1330.0
9	2485.0
10	3513.0
11	5483.0
12	9386.0
13	15748.0
14	23731.0
15	29092.0
16	34411.0
17	40633.0
18	48293.0
19	56053.0
20	65015.0
21	76778.0
22	89046.0
23	94474.0
24	96746.0
25	105609.0
26	121575.0
27	143506.0
28	177768.0
29	223982.0
30	281009.0
31	354779.0
32	469380.0
33	636858.0
34	873669.0
35	1252358.0
36	2066198.0
37	3377083.0
38	1583766.0
39	2170.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.925287364691386	19.29833290696062	12.815331747906445	24.96104798044155
2	14.993167520173856	21.578404768854117	38.44069964075685	24.987728070215177
3	17.758193041072396	27.147300806562523	29.884249783595312	25.21025636876977
4	12.519778722620742	18.202573112813166	37.76079012796315	31.516858036602947
5	13.150521430116779	38.24721096741966	34.462236912303915	14.14003069015965
6	30.117236874303543	37.71291627988812	18.37501643176822	13.794830414040113
7	26.055022028812708	32.73148539217694	23.241036605080495	17.97245597392985
8	26.492574140482972	33.42554174667555	21.016439600010614	19.06544451283087
9	27.13038776637254	14.61774525064882	20.187457475725925	38.06440950725272
10-11	24.406274319406478	25.942052485204965	27.79906067458467	21.852612520803895
12-13	25.91444097833256	23.903297945664377	27.274249156159026	22.908011919844036
14-15	23.96724673144787	24.458250503048625	25.261724824817737	26.312777940685766
16-17	22.906506819825314	27.101345820872346	26.680102104147736	23.312045255154604
18-19	23.46207740305285	26.512651274025757	28.188534747017393	21.836736575904002
20-21	24.35785404324188	26.01820645196572	27.234359065875974	22.389580438916433
22-23	23.550248853301607	25.9184155002888	26.511582714603033	24.01975293180656
24-25	23.310292961709912	26.510229204904906	26.61743449251242	23.562043340872766
26-27	23.81547615724384	25.92430585678997	26.55951770344034	23.70070028252585
28-29	23.338037915664493	25.959701619107822	27.420056086001125	23.28220437922656
30-31	24.02962640482056	26.076556727500922	26.96835504752225	22.925461820156265
32-33	22.997632518707327	26.296132687709097	26.85825675790604	23.84797803567754
34-35	22.97593967347003	26.51080451761092	27.458395723309987	23.054860085609064
36-37	23.94087871861917	25.945288003090045	26.787778887080933	23.32605439120985
38-39	24.082178558403385	25.6967303147928	26.690979804931903	23.530111321871917
40-41	23.378028127069307	26.076390567823836	27.081524719980628	23.464056585126233
42-43	24.195495309499087	25.870127919367718	26.56905152360247	23.36532524753072
44-45	24.114037585427173	26.079025933880818	26.346737767312767	23.460198713379242
46-47	23.434081480499852	25.92191039026596	27.22301273303518	23.420995396199007
48-49	24.15966373803006	26.108961985724143	26.75298471789959	22.9783895583462
50-51	23.080130122440067	26.50000200304163	26.533912081525656	23.885955792992647
52-53	22.90120776472896	26.58470569271687	25.72897910315199	24.785107439402182
54-55	23.327484052574505	26.520505179451597	25.662646181277914	24.489364586695984
56-57	23.986769271709193	25.695761941068863	25.616345693170423	24.701123094051518
58-59	23.288945769639994	25.767601902022484	26.81245128020324	24.13100104813428
60-61	23.664379996927877	26.202174410898987	26.15305038059088	23.980395211582252
62-63	22.84067631116359	26.1226408667393	26.240404358810753	24.79627846328635
64-65	22.428823828882667	26.2437402156735	26.62576385308517	24.70167210235866
66-67	23.815133425105394	25.996552600514562	26.018544882825555	24.169769091554485
68-69	23.868816257168906	25.519738756899258	26.047008610979244	24.564436374952596
70-71	22.9471974098212	25.85475629341912	26.21856122458351	24.979485072176175
72-73	23.999612750712764	25.566074955797262	26.202378289960226	24.231934003529744
74-75	24.130474146635734	25.576011978427697	26.011505109570315	24.282008765366253
76-77	23.26380804630285	25.88957479138348	26.66514847240029	24.18146868991338
78-79	23.62853823253465	26.005043996712207	26.292347667379083	24.07407010337406
80-81	22.92353470813912	25.99003768536025	26.60470244941896	24.48172515708167
82-83	23.087408785206733	25.932412604909327	26.02931811380525	24.950860496078693
84-85	23.4076067139154	26.053685155560867	25.48244751838157	25.05626061214216
86-87	23.186750943584013	25.470068491515736	26.04628487437558	25.296895690524675
88-89	23.34720186275998	24.99896022441675	26.436318479134414	25.217519433688857
90-91	23.64679173380679	25.429073732518283	26.09895373881679	24.82518079485814
92-93	23.12882842099339	25.47585513733795	26.189971125524362	25.205345316144296
94-95	22.245180085965085	25.751660440868935	26.770450146436957	25.23270932672902
96-97	23.38032367672916	25.396540447129652	26.16548731594114	25.05764856020005
98-99	23.302106690788186	25.388490767931515	25.871502417849968	25.437900123430328
100	22.889631054711256	25.6620274603872	26.156244185953355	25.292097298948192
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1515.0
1	1222.5
2	1185.5
3	1415.0
4	1179.5
5	1041.0
6	1345.0
7	2393.5
8	3838.0
9	3822.5
10	2497.0
11	1700.0
12	1648.5
13	1791.0
14	2072.0
15	2553.5
16	3092.5
17	3758.0
18	4691.5
19	5940.0
20	7468.5
21	9561.0
22	12532.0
23	16611.5
24	21469.0
25	27776.5
26	38613.0
27	51930.5
28	64408.0
29	81785.0
30	99241.0
31	113473.5
32	132564.5
33	155203.5
34	181615.5
35	203793.0
36	226491.0
37	251513.0
38	262193.0
39	274234.0
40	287024.0
41	294276.0
42	305087.5
43	332970.0
44	374906.0
45	419590.0
46	467646.5
47	508157.5
48	554041.0
49	608422.5
50	725512.0
51	720562.0
52	572058.5
53	508478.0
54	477592.0
55	436659.5
56	399148.5
57	355693.0
58	322867.0
59	296187.5
60	250634.5
61	198293.0
62	153672.0
63	117873.0
64	86964.0
65	66061.5
66	49562.0
67	34726.0
68	25968.0
69	21033.0
70	18756.5
71	17006.5
72	14909.0
73	15548.0
74	12763.5
75	9099.0
76	6532.0
77	4320.0
78	3321.5
79	2269.5
80	1502.0
81	1122.5
82	867.0
83	657.5
84	468.5
85	327.5
86	232.0
87	134.5
88	76.0
89	50.5
90	38.0
91	34.0
92	26.0
93	14.0
94	8.5
95	4.0
96	3.5
97	3.5
98	3.5
99	4.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017149053914198752
2	0.005290321349002823
3	0.008881915659335014
4	0.008582616133473999
5	0.02051415128604153
6	0.0038261804251962313
7	0.023701286777642615
8	0.008655418720845597
9	0.0011405738688217096
10-11	2.3054152667672853E-4
12-13	9.990132822658235E-4
14-15	5.905098753474099E-4
16-17	9.262106948942252E-4
18-19	0.002912103494863939
20-21	0.006115417339214273
22-23	0.01296290514033184
24-25	0.022107719031842072
26-27	0.026928868151116815
28-29	0.03263173749522536
30-31	0.034807725939998695
32-33	0.03509893628948509
34-35	0.044049609948004394
36-37	0.041181996923200875
38-39	0.04563508851743032
40-41	0.04239132879120687
42-43	0.048979962948336536
44-45	0.042504577260451586
46-47	0.04497986523108593
48-49	0.04756031249459036
50-51	0.04845012189579879
52-53	0.03366715207117699
54-55	0.029917818821539665
56-57	0.021327113511691042
58-59	0.021173419160573224
60-61	0.021569788802929705
62-63	0.0216789926839871
64-65	0.025448548874560977
66-67	0.02683179803462135
68-69	0.027834855905074486
70-71	0.028429410368609208
72-73	0.02616039639552772
74-75	0.021403960687249955
76-77	0.026026924985346457
78-79	0.025476860991872158
80-81	0.02444549100410785
82-83	0.026301956982083603
84-85	0.02765284943664549
86-87	0.030196895406464128
88-89	0.030431481521328168
90-91	0.030059379408095548
92-93	0.03277329808178125
94-95	0.03080762822274809
96-97	0.033384030898065215
98-99	0.034091833830844645
100	0.04263804867063284
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2362198E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.44948069147306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.01767097704442	23.086052527170878
2	15.452259381470729	10.33740103640277
3	6.141370124532103	6.162769241991782
4	3.0376649755767127	4.06433263790869
5	1.6701548938923352	2.793290693751045
6	1.0585260281719655	2.1244287564455897
7	0.6833480979066238	1.6000347304537716
8	0.46183489115947984	1.2358509819590067
9	0.3275286977420099	0.9860098365922205
>10	1.765895553225444	11.053321227388851
>50	0.18210585683154615	4.263118511599658
>100	0.15864399066545523	10.949542456100243
>500	0.024743585331559424	5.818693281664607
>1k	0.017098015157707883	10.188558449763331
>5k	7.281088580578869E-4	1.6741700920622054
>10k+	4.2682243403393367E-4	3.662425538745648
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	88308	0.7143389872901242	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	85736	0.6935336256545963	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	36598	0.2960476769584179	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	25563	0.20678361566446357	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	25449	0.20586144955775662	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	22043	0.17830971482579394	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	21841	0.1766757011981203	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	18473	0.1494313551683932	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	16555	0.1339163148818681	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15096	0.12211420655129453	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	14600	0.11810197506948199	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	13323	0.10777209683908963	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6178352749244108E-5	0.0
2	0.0	0.0	0.0	1.6178352749244108E-5	0.0
3	0.0	0.0	0.0	1.6178352749244108E-5	0.0
4	0.0	0.0	8.089176374622054E-6	1.6178352749244108E-5	0.0
5	0.0	0.0	8.089176374622054E-6	8.898094012084259E-5	0.0
6	0.0	0.0	8.089176374622054E-6	9.707011649546465E-5	0.0
7	0.0	0.0	8.089176374622054E-6	9.707011649546465E-5	0.0
8	0.0	0.0	8.089176374622054E-6	9.707011649546465E-5	0.0
9	8.089176374622054E-6	8.089176374622054E-6	8.089176374622054E-6	9.707011649546465E-5	0.0
10-11	4.0445881873110266E-5	8.089176374622054E-6	1.213376456193308E-5	1.213376456193308E-4	0.0
12-13	4.853505824773232E-5	8.089176374622054E-6	1.6178352749244108E-5	1.375159983685749E-4	4.044588187311027E-6
14-15	5.257964643504335E-5	8.089176374622054E-6	1.6178352749244108E-5	1.4156058655588594E-4	8.089176374622054E-6
16-17	6.471341099697643E-5	8.089176374622054E-6	2.0222940936555136E-5	1.4560517474319695E-4	1.213376456193308E-5
18-19	8.493635193353155E-5	8.089176374622054E-6	2.426752912386616E-5	2.3863070305135057E-4	1.6178352749244108E-5
20-21	9.707011649546465E-5	8.089176374622054E-6	2.426752912386616E-5	3.7210211323261444E-4	1.6178352749244108E-5
22-23	9.707011649546465E-5	8.089176374622054E-6	3.2356705498488215E-5	0.001075860457824733	1.6178352749244108E-5
24-25	1.2538223380664183E-4	8.089176374622054E-6	3.640129368579924E-5	0.0028433454956796516	1.6178352749244108E-5
26-27	1.5773893930513003E-4	8.089176374622054E-6	4.0445881873110266E-5	0.004768569472839701	1.6178352749244108E-5
28-29	1.7796188024168518E-4	8.089176374622054E-6	4.0445881873110266E-5	0.012728319025467802	1.6178352749244108E-5
30-31	1.941402329909293E-4	8.089176374622054E-6	4.853505824773232E-5	0.03933766470978704	1.6178352749244108E-5
32-33	2.0222940936555134E-4	8.089176374622054E-6	4.853505824773232E-5	0.09335718453951311	1.6178352749244108E-5
34-35	2.3458611486403956E-4	8.089176374622054E-6	5.257964643504335E-5	0.16749448601292424	1.6178352749244108E-5
36-37	2.5480905580059466E-4	8.089176374622054E-6	5.6624234622354374E-5	0.27775400458721017	1.6178352749244108E-5
38-39	2.588536439879057E-4	8.089176374622054E-6	5.6624234622354374E-5	0.45382301755723375	1.6178352749244108E-5
40-41	2.588536439879057E-4	8.089176374622054E-6	7.280258737159847E-5	0.6763077245648387	1.6178352749244108E-5
42-43	2.6694282036252774E-4	8.089176374622054E-6	7.280258737159847E-5	0.8726684364706018	1.6178352749244108E-5
44-45	2.750319967371498E-4	8.089176374622054E-6	8.089176374622053E-5	1.0908577908232824	1.6178352749244108E-5
46-47	2.750319967371498E-4	8.089176374622054E-6	8.089176374622053E-5	1.3379295494215513	1.6178352749244108E-5
48-49	2.750319967371498E-4	8.089176374622054E-6	8.089176374622053E-5	1.5943887972025688	1.6178352749244108E-5
50-51	2.750319967371498E-4	8.089176374622054E-6	8.898094012084259E-5	1.8685512074794466	1.6178352749244108E-5
52-53	2.871657612990829E-4	8.089176374622054E-6	8.898094012084259E-5	2.1513690364771705	1.6178352749244108E-5
54-55	2.9929952586101597E-4	8.089176374622054E-6	1.0515929287008669E-4	2.433475017953927	1.6178352749244108E-5
56-57	3.0738870223563804E-4	8.089176374622054E-6	1.1729305743201978E-4	2.7534140773347913	1.6178352749244108E-5
58-59	3.114332904229491E-4	8.089176374622054E-6	1.2942682199395286E-4	3.0999746161645367	1.6178352749244108E-5
60-61	3.154778786102601E-4	8.089176374622054E-6	1.2942682199395286E-4	3.444662510663557	1.6178352749244108E-5
62-63	3.154778786102601E-4	8.089176374622054E-6	1.2942682199395286E-4	3.793378814997139	1.6178352749244108E-5
64-65	3.154778786102601E-4	8.089176374622054E-6	1.6178352749244106E-4	4.160279587820871	1.6178352749244108E-5
66-67	3.195224667975711E-4	8.089176374622054E-6	1.6987270386706313E-4	4.557858562045358	1.6178352749244108E-5
68-69	3.235670549848821E-4	8.089176374622054E-6	1.6987270386706313E-4	5.002379026771776	1.6178352749244108E-5
70-71	3.235670549848821E-4	8.089176374622054E-6	1.6987270386706313E-4	5.520733448857557	1.6178352749244108E-5
72-73	3.235670549848821E-4	8.089176374622054E-6	1.6987270386706313E-4	6.0041668965340955	1.6178352749244108E-5
74-75	3.235670549848821E-4	8.089176374622054E-6	1.6987270386706313E-4	6.475628363176192	1.6178352749244108E-5
76-77	3.478345841087483E-4	8.089176374622054E-6	1.6987270386706313E-4	6.99577858241714	1.6178352749244108E-5
78-79	3.5592376048337036E-4	8.089176374622054E-6	1.6987270386706313E-4	7.5334904035673915	1.6178352749244108E-5
80-81	3.5592376048337036E-4	8.089176374622054E-6	1.6987270386706313E-4	8.116533969121026	1.6178352749244108E-5
82-83	3.599683486706814E-4	8.089176374622054E-6	1.6987270386706313E-4	8.750207689603418	1.6178352749244108E-5
84-85	3.6805752504530343E-4	8.089176374622054E-6	1.7796188024168518E-4	9.365065985838442	1.6178352749244108E-5
86-87	3.7210211323261444E-4	8.089176374622054E-6	1.8605105661630725E-4	9.996980310459353	1.6178352749244108E-5
88	3.7210211323261444E-4	8.089176374622054E-6	1.941402329909293E-4	10.498699341330724	1.6178352749244108E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGATCG	3940	0.0	20.98031	8
GTATAAG	6285	0.0	20.703331	1
GTACTAG	5845	0.0	19.77044	1
TAGTACT	6190	0.0	19.578007	4
CTGTGCG	6210	0.0	18.832294	9
TGATCGC	4445	0.0	18.702385	9
CGTTAAC	1635	0.0	18.100414	1
CATGGGG	27895	0.0	17.815527	4
GTATAAT	6520	0.0	17.507511	1
TAGGCAT	8120	0.0	17.23704	5
ATAAGGT	5650	0.0	17.209642	3
GGCGTCG	3835	0.0	17.145813	8
GTCCTAC	5010	0.0	17.06473	1
ACGTTAA	1405	0.0	17.048624	8
TACGGGC	3845	0.0	16.980787	4
CTCGTCG	3650	0.0	16.599398	9
CTAGGCA	8900	0.0	16.519379	4
CGGGCGT	3905	0.0	16.477571	6
ATACGGG	4080	0.0	16.463636	3
CTAGTAC	8185	0.0	16.183794	3
>>END_MODULE