FastQCFastQC Report
Fri 27 May 2016
ERR765527_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765527_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7780780
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA192720.2476872498644095No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT179880.23118504828564748No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA94800.12183868455347664No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA87270.11216099157153911No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC10800.024.1103151
GTACTAG36400.024.0156881
GTGATCG25750.022.2090598
GTACAAG31600.021.4834671
GTATAAG38900.021.3964631
TAGTACT43200.021.2036154
ACGTTAA7600.020.8002058
GTATCAA456200.020.5991631
TATTCGC6100.020.5799649
CTGTGCG43650.020.5581369
TGATCGC28100.020.1866479
TAGGCAT51600.019.7343045
CTAGTAC50600.019.3896733
GTACATA23350.019.117141
TACATGA27800.018.5659312
CATGGGG212550.018.3976484
ATAAGGT33650.018.3782923
GTACAAA48950.018.3334061
ATACGGG24100.018.329273
CTAGGCA57500.018.2754464
Produced by FastQC (version 0.11.5)