##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765527_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7780780 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.139092610252444 33.0 33.0 33.0 27.0 33.0 2 31.093932741961602 33.0 33.0 33.0 27.0 33.0 3 31.072508411753063 33.0 33.0 33.0 27.0 33.0 4 34.60609437614224 37.0 37.0 37.0 27.0 37.0 5 34.758006138202084 37.0 37.0 37.0 27.0 37.0 6 34.663987800708924 37.0 37.0 37.0 27.0 37.0 7 34.55983603186313 37.0 37.0 37.0 27.0 37.0 8 34.499000357290655 37.0 37.0 37.0 27.0 37.0 9 34.35086392366832 37.0 37.0 37.0 27.0 37.0 10-11 34.37595755181357 37.0 37.0 37.0 27.0 37.0 12-13 34.29507864250114 37.0 37.0 37.0 27.0 37.0 14-15 35.66231817375636 37.0 37.0 40.0 27.0 40.0 16-17 35.743536123627706 37.0 37.0 40.0 27.0 40.0 18-19 35.775682129555136 37.0 37.0 40.0 27.0 40.0 20-21 35.80067885224875 37.0 37.0 40.0 27.0 40.0 22-23 35.813426931490156 37.0 37.0 40.0 27.0 40.0 24-25 35.90377937687481 37.0 37.0 40.0 27.0 40.0 26-27 35.865121298892916 37.0 37.0 40.0 27.0 40.0 28-29 35.86350378753801 37.0 37.0 40.0 27.0 40.0 30-31 35.77856802274322 37.0 37.0 40.0 27.0 40.0 32-33 35.6786121699881 37.0 35.0 40.0 27.0 40.0 34-35 35.527198237194725 37.0 33.0 40.0 27.0 40.0 36-37 35.31054290186845 37.0 33.0 40.0 27.0 40.0 38-39 34.998680595004615 37.0 33.0 40.0 27.0 40.0 40-41 35.09866607203905 37.0 33.0 40.0 27.0 40.0 42-43 35.00394182845422 37.0 33.0 40.0 27.0 40.0 44-45 34.910555625528545 37.0 33.0 40.0 27.0 40.0 46-47 34.81166033482504 37.0 33.0 40.0 27.0 40.0 48-49 34.67565726058313 37.0 33.0 40.0 27.0 40.0 50-51 34.492191849660315 37.0 33.0 40.0 27.0 40.0 52-53 34.35085614809826 37.0 33.0 40.0 27.0 40.0 54-55 34.14316630209311 37.0 33.0 40.0 24.5 40.0 56-57 33.93409253313935 37.0 33.0 37.0 22.0 40.0 58-59 33.72636824328666 37.0 33.0 37.0 22.0 40.0 60-61 33.51956571192092 37.0 33.0 37.0 22.0 40.0 62-63 33.2951743912564 37.0 33.0 37.0 22.0 40.0 64-65 33.084033670146184 37.0 33.0 37.0 22.0 40.0 66-67 32.878794606710386 37.0 33.0 37.0 22.0 40.0 68-69 32.690468436326434 37.0 33.0 37.0 22.0 40.0 70-71 32.46659126719943 35.0 33.0 37.0 22.0 37.0 72-73 32.147018807883015 33.0 33.0 37.0 22.0 37.0 74-75 31.917787869596623 33.0 33.0 37.0 22.0 37.0 76-77 31.74040012697956 33.0 33.0 37.0 22.0 37.0 78-79 31.49830672503271 33.0 27.0 37.0 22.0 37.0 80-81 31.29936381699521 33.0 27.0 37.0 22.0 37.0 82-83 31.181778626307388 33.0 27.0 37.0 22.0 37.0 84-85 31.03849935353525 33.0 27.0 37.0 22.0 37.0 86-87 30.764969643146316 33.0 27.0 37.0 15.0 37.0 88-89 30.620196239965658 33.0 27.0 37.0 15.0 37.0 90-91 30.476629592405903 33.0 27.0 37.0 15.0 37.0 92-93 30.217853030158928 33.0 27.0 37.0 15.0 37.0 94-95 30.074256308493492 33.0 27.0 37.0 15.0 37.0 96-97 29.889963859664455 33.0 27.0 37.0 15.0 37.0 98-99 28.841970663609562 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4077022071591223 1101 2 0.4530049130096039 1101 3 0.4605533553931451 1101 4 0.4363072994732562 1101 5 0.444916078292529 1101 6 0.5190515984908046 1101 7 0.39117920836353903 1101 8 0.5037099401879104 1101 9 0.4273518733417916 1101 10-11 0.4417674821350559 1101 12-13 0.5739134647982596 1101 14-15 0.6764916278252855 1101 16-17 0.6060575571766549 1101 18-19 0.5718260484146711 1101 20-21 0.5795623102104628 1101 22-23 0.5303673649412772 1101 24-25 0.4097103206845105 1101 26-27 0.43462201516309307 1101 28-29 0.4995252945249078 1101 30-31 0.6959214386397861 1101 32-33 0.8362927426028293 1101 34-35 0.8468632924096724 1101 36-37 0.9145650645792429 1101 38-39 0.9847554963360139 1101 40-41 0.7634532092412485 1101 42-43 0.6213956193689896 1101 44-45 0.58491689133308 1101 46-47 0.6138562565443593 1101 48-49 0.460428997229279 1101 50-51 0.41272875052229097 1101 52-53 0.5256211210288129 1101 54-55 0.38247346803137106 1101 56-57 0.5867800960863576 1101 58-59 0.522592749589279 1101 60-61 0.28809710244491527 1101 62-63 0.4510808086024909 1101 64-65 0.5521084951088042 1101 66-67 0.5209189890118182 1101 68-69 0.6534524881117179 1101 70-71 0.6272687731953894 1101 72-73 0.3974691579698728 1101 74-75 0.4961585516085094 1101 76-77 0.4732669973098851 1101 78-79 0.4390432294035662 1101 80-81 0.4809477794227881 1101 82-83 0.5661427384735163 1101 84-85 0.5815235806895593 1101 86-87 0.4905145214648243 1101 88-89 0.4128209367380933 1101 90-91 0.3168061077084481 1101 92-93 0.4104661657669162 1101 94-95 0.4804758786698464 1101 96-97 0.34338707119706413 1101 98-99 0.34216996370052755 1102 1 0.3229064335934986 1102 2 0.3731599728961754 1102 3 0.3478473875585557 1102 4 0.3207246090919682 1102 5 0.3262962893647625 1102 6 0.4465440579105362 1102 7 0.38995888016415137 1102 8 0.4975733925351875 1102 9 0.4007075666403992 1102 10-11 0.35943745298386176 1102 12-13 0.5347715254170922 1102 14-15 0.6321115609963428 1102 16-17 0.41958894926044366 1102 18-19 0.4234254057546778 1102 20-21 0.47425078074545013 1102 22-23 0.495399649939678 1102 24-25 0.28076364164381573 1102 26-27 0.43310870079076125 1102 28-29 0.431716964623277 1102 30-31 0.5993103144790268 1102 32-33 0.6859567011075924 1102 34-35 0.7172637030658819 1102 36-37 0.8499668044466588 1102 38-39 0.812880669072193 1102 40-41 0.5802415730259725 1102 42-43 0.42143551216728525 1102 44-45 0.37203061024509054 1102 46-47 0.33072686424392117 1102 48-49 0.3087093944715562 1102 50-51 0.36295234224889583 1102 52-53 0.46642101315547535 1102 54-55 0.30501466964635426 1102 56-57 0.3985195790295961 1102 58-59 0.3327865905436198 1102 60-61 0.19650582024134877 1102 62-63 0.36306696841710107 1102 64-65 0.35537219791919483 1102 66-67 0.44139408388845425 1102 68-69 0.49999880156536136 1102 70-71 0.5278596658359831 1102 72-73 0.3243642131322346 1102 74-75 0.39317323870648835 1102 76-77 0.38406154954495975 1102 78-79 0.3631528197676346 1102 80-81 0.32856003722676874 1102 82-83 0.26605137463696593 1102 84-85 0.4141401228081456 1102 86-87 0.41590432495337737 1102 88-89 0.25894328540803 1102 90-91 0.3456605266507289 1102 92-93 0.35243170314485894 1102 94-95 0.3915410727209654 1102 96-97 0.2556985118772772 1102 98-99 0.29226252194319 1103 1 0.1900699845719238 1103 2 0.3011643184742603 1103 3 0.1942737302691384 1103 4 0.222231749970085 1103 5 0.21326939867349637 1103 6 0.34734876644885304 1103 7 0.2799797185753832 1103 8 0.2371107634949965 1103 9 0.24142035617850865 1103 10-11 0.2747785320924194 1103 12-13 0.3587028512976289 1103 14-15 0.5000767999442104 1103 16-17 0.2645298983103004 1103 18-19 0.26289354302110013 1103 20-21 0.3157249095730492 1103 22-23 0.24420783932122703 1103 24-25 0.1507268521984173 1103 26-27 0.3296398314719724 1103 28-29 0.2636408431250743 1103 30-31 0.5107579784086838 1103 32-33 0.5766541183952896 1103 34-35 0.6723159837249639 1103 36-37 0.743575326099581 1103 38-39 0.7498078893070854 1103 40-41 0.5215234930851977 1103 42-43 0.39372928926977835 1103 44-45 0.26745120235185027 1103 46-47 0.2258839655244671 1103 48-49 0.19211851070068775 1103 50-51 0.1356107121171206 1103 52-53 0.30063783726141935 1103 54-55 0.15869694808590395 1103 56-57 0.22133847024421271 1103 58-59 0.16993744415155732 1103 60-61 0.047933902158298736 1103 62-63 0.14221285915570547 1103 64-65 0.2844780042593271 1103 66-67 0.21720472411830372 1103 68-69 0.36230975667084664 1103 70-71 0.34352739318646996 1103 72-73 0.06909196975748699 1103 74-75 0.25056333864630886 1103 76-77 0.15902016387136086 1103 78-79 0.1267303830054658 1103 80-81 0.09284715229842178 1103 82-83 0.15829173086320836 1103 84-85 0.3188146185902063 1103 86-87 0.1674612759346381 1103 88-89 0.21194598636469664 1103 90-91 0.15494942529289446 1103 92-93 0.19288518570337132 1103 94-95 0.21382726472551283 1103 96-97 0.08404655221843171 1103 98-99 0.05324795977040253 1104 1 0.1175147163117245 1104 2 0.1997325125099252 1104 3 0.07081872112994247 1104 4 0.17675713186004316 1104 5 0.15324160850781254 1104 6 0.16198139556976088 1104 7 0.08959838972211287 1104 8 0.09020741892767603 1104 9 0.06780737700178463 1104 10-11 0.13029765356139222 1104 12-13 0.14816512946143945 1104 14-15 0.25734035228369123 1104 16-17 0.16749863564619716 1104 18-19 0.1673699159296902 1104 20-21 0.11996706500592325 1104 22-23 0.061724636441887526 1104 24-25 0.0074515789948179645 1104 26-27 0.17819117785703753 1104 28-29 0.09328210050428964 1104 30-31 0.295904096103051 1104 32-33 0.4027585546868906 1104 34-35 0.46628465782251993 1104 36-37 0.5668076377218725 1104 38-39 0.5242423826545846 1104 40-41 0.31269645910765576 1104 42-43 0.2539706757800957 1104 44-45 0.2348773477484798 1104 46-47 0.2548313680354468 1104 48-49 0.06027241590455645 1104 50-51 -0.05407242887135766 1104 52-53 0.15840000372153185 1104 54-55 -0.004145917796464005 1104 56-57 0.1878071997873647 1104 58-59 0.0903838659768752 1104 60-61 -0.02426787339004477 1104 62-63 0.06549887564618473 1104 64-65 0.08824762885180348 1104 66-67 0.001209385653439199 1104 68-69 0.06890762512569637 1104 70-71 0.09297220721995103 1104 72-73 -0.0927553334198663 1104 74-75 -0.05527956046004334 1104 76-77 0.03795851002917772 1104 78-79 0.06408326967611089 1104 80-81 -0.06351419700219907 1104 82-83 0.023170457417350576 1104 84-85 0.10608206845792623 1104 86-87 -0.03308108164401702 1104 88-89 -0.09457312478141944 1104 90-91 -0.08775956704410603 1104 92-93 -0.0015107649385797117 1104 94-95 -0.05199386906108572 1104 96-97 -0.14575663084681167 1104 98-99 -0.08390577387745424 1105 1 0.03870619651818785 1105 2 0.0889747031497663 1105 3 0.08358404176386358 1105 4 0.10719368146135366 1105 5 -0.015073516125937658 1105 6 0.19066163383813262 1105 7 0.07791794689570253 1105 8 0.1324779156569562 1105 9 0.050942424848791745 1105 10-11 0.12876636741613368 1105 12-13 0.14924449601114276 1105 14-15 0.20223895981320084 1105 16-17 0.018576886476310506 1105 18-19 0.18656443954130708 1105 20-21 0.19943154498128024 1105 22-23 0.08878506110688278 1105 24-25 -0.04307487407152166 1105 26-27 0.07233195873060794 1105 28-29 0.04520700883346507 1105 30-31 0.31076203246385603 1105 32-33 0.3861915223463228 1105 34-35 0.4022860183482848 1105 36-37 0.47552459677441306 1105 38-39 0.44170010797567727 1105 40-41 0.28707438766777216 1105 42-43 0.15725148962651048 1105 44-45 0.12882134659265887 1105 46-47 0.16204289955168605 1105 48-49 -0.004888787074797563 1105 50-51 -0.020945491611072953 1105 52-53 0.025904064012237882 1105 54-55 -0.03466225198081929 1105 56-57 0.18423919022102808 1105 58-59 0.022733856946921094 1105 60-61 -0.052905265616253416 1105 62-63 -0.05038762870752578 1105 64-65 0.009435469147163644 1105 66-67 0.03546426650487433 1105 68-69 0.16056350000530273 1105 70-71 0.14437906511301435 1105 72-73 -0.03199670747206085 1105 74-75 0.09682032020332798 1105 76-77 0.049485913555336936 1105 78-79 0.051851810940235055 1105 80-81 -0.08989354007359651 1105 82-83 0.1140664911079412 1105 84-85 0.07299927796753636 1105 86-87 0.015113021297377571 1105 88-89 -0.0716936997748121 1105 90-91 -0.07766722770946188 1105 92-93 -0.04332388397776299 1105 94-95 -0.05170804566208531 1105 96-97 -0.16746021216633267 1105 98-99 -0.10613627751767041 1106 1 0.13950945051545105 1106 2 0.15881569866994028 1106 3 0.10579209422305524 1106 4 0.0059354021304045546 1106 5 0.021790830304468045 1106 6 0.10703397246002311 1106 7 0.08818526152815309 1106 8 0.10150299426806697 1106 9 0.09476687902244407 1106 10-11 0.06672227941819386 1106 12-13 0.06545125736126067 1106 14-15 0.21152198172602255 1106 16-17 -0.041351444123627346 1106 18-19 0.2039364730752098 1106 20-21 0.10104103070008819 1106 22-23 0.02127712419481753 1106 24-25 0.06338872266371709 1106 26-27 0.17260302123221294 1106 28-29 0.06617631811861457 1106 30-31 0.30667364384392926 1106 32-33 0.3515238912948675 1106 34-35 0.41330284332286027 1106 36-37 0.4759563703213061 1106 38-39 0.4191015299056815 1106 40-41 0.27337390015554774 1106 42-43 0.13691893785937026 1106 44-45 0.08634714681691236 1106 46-47 0.11907534003403697 1106 48-49 0.06529381077299234 1106 50-51 -0.013282683972072107 1106 52-53 0.048267884395656324 1106 54-55 -0.07763411829938605 1106 56-57 0.011363629826341537 1106 58-59 -0.05228734884488517 1106 60-61 -0.1409688331795209 1106 62-63 -0.040473341241494154 1106 64-65 0.13149114903558257 1106 66-67 0.08527638028650131 1106 68-69 0.08640472316159986 1106 70-71 0.09378736392827847 1106 72-73 -0.09232146128407237 1106 74-75 0.05213607548213872 1106 76-77 0.10543426981308812 1106 78-79 0.059720385605068316 1106 80-81 0.012017316596939054 1106 82-83 -0.0838904739968882 1106 84-85 -0.008799340437537495 1106 86-87 -0.1178064669147112 1106 88-89 -0.10905821358492318 1106 90-91 -0.17086235386123505 1106 92-93 -0.16063004779575607 1106 94-95 -0.013328393125753735 1106 96-97 -0.14451653976798084 1106 98-99 -0.18336648770923603 1107 1 0.08706917350717802 1107 2 0.15185092230253971 1107 3 0.10601467200885395 1107 4 0.03197922968432465 1107 5 0.022366190530362928 1107 6 0.06826903710530274 1107 7 0.05739380122243887 1107 8 0.017912834685489543 1107 9 -0.04479289662629782 1107 10-11 0.030777017499538317 1107 12-13 0.03150324507692659 1107 14-15 0.10615762395674722 1107 16-17 -0.002329641803072491 1107 18-19 0.23597429010756343 1107 20-21 0.15637135336081087 1107 22-23 -0.05114585389595305 1107 24-25 0.02527618214769234 1107 26-27 0.06122923425602522 1107 28-29 0.060043032554439435 1107 30-31 0.24741946984036645 1107 32-33 0.30452968186313 1107 34-35 0.3185469540855195 1107 36-37 0.459645769945368 1107 38-39 0.3450636588203224 1107 40-41 0.2113112730430231 1107 42-43 0.10779542890997362 1107 44-45 0.09346817714545352 1107 46-47 0.013541438410740625 1107 48-49 -0.1256200583805338 1107 50-51 -0.04185710885194993 1107 52-53 0.002350337766834798 1107 54-55 -0.043391794915649484 1107 56-57 0.021025915919089755 1107 58-59 0.015604428402440362 1107 60-61 -0.07277020877835838 1107 62-63 -0.01351786123792209 1107 64-65 0.11237609159253026 1107 66-67 0.10265543500210583 1107 68-69 0.15180978625341623 1107 70-71 0.04981026380970377 1107 72-73 -0.26997232097262014 1107 74-75 -0.023644848116276762 1107 76-77 -0.04759989369927453 1107 78-79 -0.0598980593367493 1107 80-81 -0.02878369805288017 1107 82-83 -0.09966959033739187 1107 84-85 -0.019971578929016687 1107 86-87 -0.20422805422936463 1107 88-89 -0.3001883820270521 1107 90-91 -0.14137766554886966 1107 92-93 -0.19836215814810387 1107 94-95 -0.13437778051753213 1107 96-97 -0.25220557481343064 1107 98-99 -0.3033478535204637 1108 1 -0.0025010071711939474 1108 2 -0.0036738395660407264 1108 3 -0.03367948666770104 1108 4 -0.07118527535666175 1108 5 -0.11717587820719189 1108 6 0.03355387402493193 1108 7 -0.04252389882618246 1108 8 -0.030475563229167335 1108 9 -0.05862625845090719 1108 10-11 -0.03497253701039682 1108 12-13 -0.11812970319841298 1108 14-15 -0.1167029730195992 1108 16-17 -0.1415334380094322 1108 18-19 0.14182269680102166 1108 20-21 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92-93 0.14206576181289776 2215 94-95 0.267864101794558 2215 96-97 0.3334105555142095 2215 98-99 0.374318734674727 2216 1 -0.37184051560952724 2216 2 -0.3944459480643019 2216 3 -0.2522622187460435 2216 4 -0.294936976016146 2216 5 -0.3906004168758699 2216 6 -0.3388616182471651 2216 7 -0.49511858804238784 2216 8 -0.4446975883361617 2216 9 -0.32451714577106827 2216 10-11 -0.4186217099170477 2216 12-13 -0.5071690743239898 2216 14-15 -0.5411792233989203 2216 16-17 -0.5873146518788417 2216 18-19 -0.49417843084075486 2216 20-21 -0.27056743474281575 2216 22-23 -0.2503116608244227 2216 24-25 -0.4279059876298561 2216 26-27 -0.46972135681885874 2216 28-29 -0.46931844395316347 2216 30-31 -0.32953168836733226 2216 32-33 -0.2877883122027711 2216 34-35 -0.387223271520007 2216 36-37 -0.22965416045669684 2216 38-39 -8.54867568475725 2216 40-41 -2.012476084963126 2216 42-43 -0.7390234792632882 2216 44-45 -0.7883687781702591 2216 46-47 -0.5142014813241431 2216 48-49 -0.3079988594038312 2216 50-51 -0.37905380877186445 2216 52-53 -0.4069185779793614 2216 54-55 -0.4275250709511482 2216 56-57 -0.3507935613665083 2216 58-59 -0.4419074110376897 2216 60-61 -0.44592418789004995 2216 62-63 -0.419411471287674 2216 64-65 -0.5193946481047931 2216 66-67 -0.38047716007810095 2216 68-69 -0.3308521713184476 2216 70-71 -0.12209273340871363 2216 72-73 -0.1921716594927716 2216 74-75 -0.2596835135454185 2216 76-77 -0.37031792197029034 2216 78-79 -0.3895437392550747 2216 80-81 -0.3495473774071911 2216 82-83 -0.42625347757289944 2216 84-85 -0.3680468961828183 2216 86-87 -0.28849442832110483 2216 88-89 -0.26869542752366016 2216 90-91 -0.331426876445736 2216 92-93 -0.2501785458579384 2216 94-95 -0.059292425720840924 2216 96-97 -0.06052333506742258 2216 98-99 -0.026871824177053583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 257.0 8 1517.0 9 2851.0 10 4673.0 11 8833.0 12 18073.0 13 31160.0 14 40682.0 15 43319.0 16 45859.0 17 48608.0 18 51946.0 19 56068.0 20 60650.0 21 66422.0 22 73827.0 23 82889.0 24 92683.0 25 103351.0 26 112197.0 27 124988.0 28 146655.0 29 178631.0 30 220195.0 31 279320.0 32 363913.0 33 484780.0 34 654216.0 35 927056.0 36 1367100.0 37 1566988.0 38 520474.0 39 596.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52140787941569 19.823359611761287 12.626330522132742 24.02890198669028 2 15.580650654671057 21.388268937264037 38.52392320668327 24.507157201381638 3 17.435648878627052 27.96428767895876 29.32682148877856 25.273241953635626 4 12.214276717758374 18.097105945676397 38.04001655361031 31.648600782954922 5 13.556510297810778 38.73353655487939 33.33408308605417 14.375870061255656 6 29.725837255390847 38.75211739697048 18.114983330720055 13.40706201691861 7 26.535570074052217 33.24086981086341 22.590501194875646 17.63305892020873 8 26.40212163819052 33.763851439058804 20.28569115178684 19.54833577096384 9 26.75393726592964 15.94149943835965 20.035433465539445 37.269129830171266 10-11 25.077914761357107 26.579583264065416 26.56450765177289 21.777994322804588 12-13 25.538321983457816 24.784997330921577 26.454708788346355 23.221971897274248 14-15 23.955364663889316 25.6168963116032 23.899166923931716 26.52857210057577 16-17 23.427436726040412 27.719596574528378 24.881072151232374 23.97189454819884 18-19 23.895033659864435 27.17329753572264 26.40681911068042 22.524849693732506 20-21 25.9854217700539 26.243943409272593 24.739743316222796 23.03089150445071 22-23 24.92254946905661 26.19838028946481 24.803634548906643 24.075435692571933 24-25 23.96797771513422 26.91934209316339 25.0275794363918 24.085100755310588 26-27 24.328733252551093 26.70580295473812 24.879880543732686 24.0855832489781 28-29 23.60370437345715 26.68297509164091 25.67199154928238 24.041328985619565 30-31 24.208700113660907 26.75050296574191 25.274614121958084 23.7661827986391 32-33 23.820821585268394 26.972858789052008 24.550195521901973 24.65612410377762 34-35 24.291846337503845 26.929808253787424 25.509429999911966 23.26891540879677 36-37 24.137678200619554 26.799755088412237 25.043585034740147 24.018981676228062 38-39 24.21496936485418 26.371231981874377 25.18909414914915 24.22470450412229 40-41 23.97213758059234 26.871643217493546 25.58097046519794 23.575248736716176 42-43 24.594592579921247 26.546775100044943 25.302891635295456 23.555740684738353 44-45 23.86831061517105 27.10244694008145 25.004662129588823 24.024580315158676 46-47 23.400011875516824 26.84675211041043 25.673543584817537 24.079692429255207 48-49 24.223870145491393 26.94194321011566 25.322900260700436 23.51128638369251 50-51 23.38602065401577 27.26548442305652 25.086834891577116 24.26166003135059 52-53 23.499694020676273 26.789374989276425 24.988939054689364 24.72199193535794 54-55 23.957540246607667 26.626070908058065 25.233369919211185 24.183018926123086 56-57 24.235757433448192 26.21045959235513 24.759332110056842 24.79445086413984 58-59 23.693485743074604 26.30111634052113 25.695097406686735 24.310300509717532 60-61 23.82244241762315 26.556022890291263 25.31894032027181 24.302594371813775 62-63 23.428064685073142 26.56198771973229 24.945490648425125 25.064456946769443 64-65 23.465061519378867 26.360475169454133 25.201167141494253 24.97329616967275 66-67 24.36569292730432 26.3396732070371 24.97976557433863 24.31486829131995 68-69 24.334493836685517 25.96306708845524 25.042787094770887 24.659651980088363 70-71 23.505776963462957 26.179122656748373 25.285574077490615 25.029526302298056 72-73 24.321103064016018 26.25192392233841 25.233282110136106 24.19369090350947 74-75 24.69591531293357 26.038021269444883 25.27152311922726 23.994540298394284 76-77 23.99405174399237 26.416666066897253 25.362579889285993 24.22670229982439 78-79 24.111649161534995 26.478909660051464 25.26841187615718 24.14102930225636 80-81 23.764012091332745 26.08531535398765 25.940953220628266 24.209719334051343 82-83 24.206754051536407 26.208546423276314 25.361623143881207 24.223076381306075 84-85 24.322013525317608 26.35406921049877 24.884794077788086 24.43912318639554 86-87 23.93396291888474 25.923988340500564 25.324061340893845 24.81798739972085 88-89 24.14325892968165 25.405761133028015 25.51510751784028 24.93587241945006 90-91 24.537739567724532 25.660538666472682 25.257331153761477 24.54439061204131 92-93 24.30770436897072 25.792671171784832 25.178703163436055 24.72092129580839 94-95 23.470738152216104 26.117349417410594 25.779761155051297 24.632151275322013 96-97 24.18568424732537 26.005909630718772 25.13555649236812 24.67284962958774 98-99 23.77767461277135 26.21205601345738 25.117435880035206 24.892833493736067 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1814.0 1 1508.0 2 1177.0 3 1134.0 4 989.0 5 797.5 6 692.0 7 671.0 8 670.5 9 634.0 10 595.0 11 572.0 12 588.0 13 628.0 14 726.5 15 944.5 16 1159.0 17 1343.0 18 1744.5 19 2356.5 20 3268.5 21 4644.5 22 6486.5 23 9190.5 24 12749.5 25 18004.0 26 25351.5 27 32859.0 28 42892.0 29 55686.0 30 66765.5 31 79547.5 32 93999.0 33 107564.5 34 120624.5 35 135061.0 36 146004.0 37 158198.0 38 172957.0 39 170038.0 40 167011.5 41 176551.5 42 188808.0 43 210749.0 44 236401.0 45 266252.0 46 306925.5 47 338152.0 48 351116.5 49 360511.5 50 377368.5 51 364222.5 52 337137.5 53 328889.0 54 305859.5 55 290003.5 56 265844.0 57 239062.5 58 231231.5 59 206479.5 60 166493.5 61 120906.0 62 94429.5 63 76268.5 64 55750.5 65 44683.5 66 33515.5 67 24658.5 68 18730.0 69 15605.5 70 15044.0 71 14727.5 72 15293.0 73 14133.5 74 10133.0 75 7400.5 76 5962.5 77 4497.0 78 2838.5 79 1945.0 80 1586.0 81 1323.5 82 1193.5 83 985.0 84 687.5 85 539.5 86 334.5 87 188.0 88 135.0 89 118.0 90 103.5 91 78.0 92 53.0 93 32.0 94 26.5 95 24.5 96 16.0 97 12.5 98 10.0 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.001992088196813173 3 0.006374682229802154 4 0.0 5 0.005616403496821656 6 0.0 7 6.940178234058796E-4 8 0.0 9 0.0 10-11 1.9278272872385543E-5 12-13 5.7834818617156635E-5 14-15 0.002801775657453366 16-17 1.5422618297908434E-4 18-19 0.0 20-21 0.0 22-23 8.996527340446588E-5 24-25 9.446353707468917E-4 26-27 0.0024676189276653495 28-29 0.0017864532861743936 30-31 0.0023198188356437274 32-33 8.48244006384964E-4 34-35 6.4260909574618476E-6 36-37 1.6707836489400805E-4 38-39 0.0620182038304643 40-41 0.003392976025539856 42-43 3.470089117029398E-4 44-45 1.2852181914923695E-5 46-47 8.48244006384964E-4 48-49 1.349479101066988E-4 50-51 8.418179154275022E-4 52-53 3.277306388305543E-4 54-55 0.0 56-57 4.755307308521768E-4 58-59 0.0 60-61 1.7993054680893176E-4 62-63 6.426090957461849E-5 64-65 7.839830968103456E-4 66-67 0.0013430530101095264 68-69 0.0013430530101095264 70-71 7.068700053208033E-5 72-73 0.001612948830322924 74-75 0.0017350445585146992 76-77 1.5422618297908434E-4 78-79 1.7993054680893176E-4 80-81 0.0 82-83 3.598610936178635E-4 84-85 4.948090037245622E-4 86-87 0.0 88-89 1.9278272872385543E-5 90-91 6.040525500014138E-4 92-93 0.0 94-95 0.0 96-97 0.003341567297880161 98-99 0.004678194217032226 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7780780.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.51767123362119 #Duplication Level Percentage of deduplicated Percentage of total 1 75.23966742312548 27.47577438684695 2 14.04720349260222 10.259423177892465 3 4.801365291841617 5.260040375999756 4 2.050062939112964 2.9945409767503364 5 1.0478071223640018 1.9131738005367656 6 0.5973608145605859 1.308853550038298 7 0.3796253637698836 0.9704123958264723 8 0.2545176515124203 0.7435513536867148 9 0.18282383816576503 0.6008670734225557 >10 1.0675690212317839 7.524402509222402 >50 0.13244979890284933 3.392412874738737 >100 0.14604799724052647 11.65951056767071 >500 0.02982105517359914 7.586471356987288 >1k 0.022392496800366438 15.069974393028133 >5k 0.0012142661741449106 2.7546531592195462 >10k+ 7.142742200852415E-5 0.4859380481330588 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 19272 0.2476872498644095 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 17988 0.23118504828564748 No Hit GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA 9480 0.12183868455347664 No Hit GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA 8727 0.11216099157153911 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2852181914923697E-5 0.0 0.0 0.0 1.2852181914923697E-5 2 1.2852181914923697E-5 0.0 0.0 0.0 1.2852181914923697E-5 3 1.2852181914923697E-5 0.0 0.0 0.0 1.2852181914923697E-5 4 1.2852181914923697E-5 0.0 0.0 1.2852181914923697E-5 1.2852181914923697E-5 5 2.5704363829847394E-5 0.0 0.0 2.5704363829847394E-5 1.2852181914923697E-5 6 2.5704363829847394E-5 0.0 0.0 3.855654574477109E-5 2.5704363829847394E-5 7 2.5704363829847394E-5 0.0 0.0 3.855654574477109E-5 2.5704363829847394E-5 8 2.5704363829847394E-5 0.0 0.0 3.855654574477109E-5 2.5704363829847394E-5 9 2.5704363829847394E-5 0.0 0.0 3.855654574477109E-5 3.855654574477109E-5 10-11 2.5704363829847394E-5 0.0 0.0 7.068700053208033E-5 3.855654574477109E-5 12-13 2.5704363829847394E-5 0.0 0.0 8.996527340446588E-5 3.855654574477109E-5 14-15 2.5704363829847394E-5 0.0 0.0 8.996527340446588E-5 5.140872765969479E-5 16-17 3.855654574477109E-5 0.0 0.0 1.6707836489400805E-4 5.140872765969479E-5 18-19 5.1408727659694794E-5 0.0 0.0 2.1848709255370283E-4 5.140872765969479E-5 20-21 7.711309148954218E-5 0.0 0.0 3.727132755327872E-4 5.140872765969479E-5 22-23 7.711309148954218E-5 0.0 0.0 0.001445870465428916 5.140872765969479E-5 24-25 8.353918244700403E-5 0.0 0.0 0.003682150118625639 5.140872765969479E-5 26-27 1.413740010641607E-4 0.0 0.0 0.005931281953737285 5.7834818617156635E-5 28-29 1.5422618297908437E-4 0.0 0.0 0.015236261660142043 6.426090957461849E-5 30-31 1.7993054680893176E-4 0.0 0.0 0.04595940252776714 6.426090957461849E-5 32-33 2.1206100159624098E-4 0.0 0.0 0.10063901048481 8.996527340446588E-5 34-35 2.3133927446862654E-4 0.0 0.0 0.17258552484455286 1.2209572819177512E-4 36-37 2.377653654260884E-4 0.0 0.0 0.28522847323790157 1.4780009202162251E-4 38-39 2.5704363829847396E-4 0.0 0.0 0.46143831338246294 1.5422618297908437E-4 40-41 2.7632191117085947E-4 0.0 0.0 0.6733129583409375 1.5422618297908437E-4 42-43 2.891740930857832E-4 0.0 0.0 0.8661021131557505 1.5422618297908437E-4 44-45 2.9560018404324503E-4 6.426090957461848E-6 0.0 1.0929816804998986 1.6707836489400805E-4 46-47 2.9560018404324503E-4 1.2852181914923697E-5 0.0 1.3378543025249396 1.6707836489400805E-4 48-49 2.9560018404324503E-4 1.2852181914923697E-5 0.0 1.6033482504324759 2.0563491063877915E-4 50-51 2.9560018404324503E-4 1.2852181914923697E-5 0.0 1.9006127920337037 2.0563491063877915E-4 52-53 2.9560018404324503E-4 1.2852181914923697E-5 0.0 2.19428514878971 2.1206100159624098E-4 54-55 2.9560018404324503E-4 1.2852181914923697E-5 0.0 2.477476551194096 2.1848709255370283E-4 56-57 2.9560018404324503E-4 1.9278272872385546E-5 0.0 2.7995072473453817 2.1848709255370283E-4 58-59 2.9560018404324503E-4 2.5704363829847394E-5 0.0 3.1550435817488736 2.3133927446862654E-4 60-61 2.9560018404324503E-4 2.5704363829847394E-5 0.0 3.514474127272587 2.3133927446862654E-4 62-63 2.9560018404324503E-4 2.5704363829847394E-5 0.0 3.876610057089392 2.4419145638355025E-4 64-65 2.9560018404324503E-4 2.5704363829847394E-5 0.0 4.255871519307833 2.4419145638355025E-4 66-67 2.9560018404324503E-4 2.5704363829847394E-5 0.0 4.664294582291236 2.4419145638355025E-4 68-69 2.9560018404324503E-4 2.5704363829847394E-5 0.0 5.101416567490663 2.4419145638355025E-4 70-71 2.9560018404324503E-4 2.5704363829847394E-5 0.0 5.598461850868421 2.5704363829847396E-4 72-73 2.9560018404324503E-4 2.5704363829847394E-5 0.0 6.083214022244556 2.827480021283213E-4 74-75 2.9560018404324503E-4 2.5704363829847394E-5 0.0 6.553192610509486 2.827480021283213E-4 76-77 2.9560018404324503E-4 2.5704363829847394E-5 0.0 7.057248759121836 2.9560018404324503E-4 78-79 2.9560018404324503E-4 2.5704363829847394E-5 0.0 7.5814249985219995 2.9560018404324503E-4 80-81 2.9560018404324503E-4 2.5704363829847394E-5 0.0 8.154619459745682 2.9560018404324503E-4 82-83 2.9560018404324503E-4 2.5704363829847394E-5 0.0 8.767180154174774 2.9560018404324503E-4 84-85 2.9560018404324503E-4 3.2130454787309245E-5 0.0 9.357423034708603 2.9560018404324503E-4 86-87 2.9560018404324503E-4 4.498263670223294E-5 0.0 9.960929366978633 3.0845236595816874E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAAC 1080 0.0 24.110315 1 GTACTAG 3640 0.0 24.015688 1 GTGATCG 2575 0.0 22.209059 8 GTACAAG 3160 0.0 21.483467 1 GTATAAG 3890 0.0 21.396463 1 TAGTACT 4320 0.0 21.203615 4 ACGTTAA 760 0.0 20.800205 8 GTATCAA 45620 0.0 20.599163 1 TATTCGC 610 0.0 20.579964 9 CTGTGCG 4365 0.0 20.558136 9 TGATCGC 2810 0.0 20.186647 9 TAGGCAT 5160 0.0 19.734304 5 CTAGTAC 5060 0.0 19.389673 3 GTACATA 2335 0.0 19.11714 1 TACATGA 2780 0.0 18.565931 2 CATGGGG 21255 0.0 18.397648 4 ATAAGGT 3365 0.0 18.378292 3 GTACAAA 4895 0.0 18.333406 1 ATACGGG 2410 0.0 18.32927 3 CTAGGCA 5750 0.0 18.275446 4 >>END_MODULE