FastQCFastQC Report
Fri 27 May 2016
ERR765527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765527_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7780780
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236500.3039541022879454No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT198630.25528288937612936No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA184730.23741835651438548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154570.19865617585897558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122500.1574392284578153No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA88530.11378036649281949No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA82570.10612046607152498No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT80410.10334439477790146No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC80120.10297168150236866No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT79330.10195635913108968No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA79260.10186639385768523No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA78160.10045265384704362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA567100.029.9893281
TATCACG4800.029.0648272
GTACTAG33750.024.5406041
CAACGCA694550.024.3302655
ATCAACG694600.024.2883423
AACGCAG729550.023.4110076
TATCAAC736300.023.1160722
TCAACGC732200.023.0982464
AGTACTT361800.022.49394612-13
ACGCAGA772050.021.6946437
CGCAGAG776700.021.541238
GGTATCA385550.021.1442571
GCAGAGT806250.020.6715039
TAGGCAT51100.020.2931465
GAGTACT350600.020.20815512-13
TAACGCA6600.019.0232734
TACTTTT434350.018.95605914-15
TCTAACG16150.018.716672
CTAGGCA55950.018.7002684
ACAACGC7800.018.4812983
Produced by FastQC (version 0.11.5)