##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765527_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7780780 Sequences flagged as poor quality 0 Sequence length 99 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29721441809176 33.0 33.0 33.0 33.0 33.0 2 32.140619320942115 33.0 33.0 33.0 33.0 33.0 3 32.218014260781054 33.0 33.0 33.0 33.0 33.0 4 36.13826390156257 37.0 37.0 37.0 33.0 37.0 5 36.08930132968674 37.0 37.0 37.0 33.0 37.0 6 36.08682934101722 37.0 37.0 37.0 33.0 37.0 7 35.99867802456823 37.0 37.0 37.0 33.0 37.0 8 35.960247301684404 37.0 37.0 37.0 33.0 37.0 9 35.69852045681795 37.0 37.0 37.0 33.0 37.0 10-11 35.87908975706806 37.0 37.0 37.0 33.0 37.0 12-13 35.79989814645832 37.0 37.0 37.0 33.0 37.0 14-15 37.16871027069266 37.0 37.0 40.0 33.0 40.0 16-17 37.32255512943432 37.0 37.0 40.0 33.0 40.0 18-19 37.40001240235554 37.0 37.0 40.0 33.0 40.0 20-21 37.4277249196096 37.0 37.0 40.0 33.0 40.0 22-23 37.34142033317996 37.0 37.0 40.0 33.0 40.0 24-25 37.28109932423227 37.0 37.0 40.0 33.0 40.0 26-27 37.14453332442248 37.0 37.0 40.0 33.0 40.0 28-29 37.07388173165158 37.0 37.0 40.0 33.0 40.0 30-31 36.9628828986297 37.0 37.0 40.0 33.0 40.0 32-33 36.77837851732089 37.0 37.0 40.0 33.0 40.0 34-35 36.682435565585976 37.0 37.0 40.0 33.0 40.0 36-37 36.51579835183619 37.0 37.0 40.0 33.0 40.0 38-39 36.35003521497845 37.0 37.0 40.0 33.0 40.0 40-41 36.205541475276256 37.0 37.0 40.0 33.0 40.0 42-43 36.0093552959986 37.0 37.0 40.0 33.0 40.0 44-45 35.776990802978624 37.0 37.0 40.0 30.0 40.0 46-47 35.4938146304098 37.0 37.0 40.0 27.0 40.0 48-49 35.22071508254956 37.0 33.0 40.0 27.0 40.0 50-51 34.90409367698354 37.0 33.0 40.0 27.0 40.0 52-53 34.744826996779246 37.0 33.0 40.0 27.0 40.0 54-55 34.609490822256895 37.0 33.0 40.0 27.0 40.0 56-57 34.42864449322562 37.0 33.0 40.0 27.0 40.0 58-59 34.24235944211249 37.0 33.0 37.0 27.0 40.0 60-61 34.07720755502662 37.0 33.0 37.0 27.0 40.0 62-63 33.76972013088662 37.0 33.0 37.0 27.0 40.0 64-65 33.63643876320883 37.0 33.0 37.0 27.0 40.0 66-67 33.46434907554256 37.0 33.0 37.0 27.0 40.0 68-69 33.235500296885405 37.0 33.0 37.0 24.5 40.0 70-71 33.061382020825675 37.0 33.0 37.0 22.0 38.5 72-73 32.8735066407224 37.0 33.0 37.0 22.0 37.0 74-75 32.68250374641103 37.0 33.0 37.0 22.0 37.0 76-77 32.52608562380635 35.0 33.0 37.0 22.0 37.0 78-79 32.34658350448156 33.0 33.0 37.0 22.0 37.0 80-81 32.20953766845997 33.0 33.0 37.0 22.0 37.0 82-83 32.07089456327 33.0 33.0 37.0 22.0 37.0 84-85 31.91887445731662 33.0 33.0 37.0 22.0 37.0 86-87 31.82220805626171 33.0 33.0 37.0 22.0 37.0 88-89 31.713779788144635 33.0 33.0 37.0 22.0 37.0 90-91 31.658631525374062 33.0 33.0 37.0 22.0 37.0 92-93 31.557500212061 33.0 33.0 37.0 22.0 37.0 94-95 31.492332773835017 33.0 33.0 37.0 22.0 37.0 96-97 31.45205808415095 33.0 33.0 37.0 22.0 37.0 98-99 30.634229987224934 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15791117270070743 1101 2 0.36393010346482413 1101 3 0.24745445699780078 1101 4 0.20039521934004512 1101 5 0.07455230236701738 1101 6 0.1501915531018767 1101 7 0.157708919846705 1101 8 0.1550105756970055 1101 9 0.42746814304639 1101 10-11 0.3434084754775668 1101 12-13 0.3110470410997195 1101 14-15 0.5501780721653304 1101 16-17 0.44839536670055935 1101 18-19 0.2802893246472422 1101 20-21 0.3861382873405006 1101 22-23 0.2546103755689586 1101 24-25 0.3637595157881037 1101 26-27 0.24269375050041475 1101 28-29 0.21821023844660203 1101 30-31 0.3192554034404651 1101 32-33 0.40405621679928316 1101 34-35 0.45438872242728934 1101 36-37 0.4366684997678334 1101 38-39 0.2078547879019581 1101 40-41 0.13678533448663188 1101 42-43 0.23894258122872003 1101 44-45 0.39590997148028606 1101 46-47 0.4863720901054478 1101 48-49 0.5185006235854459 1101 50-51 0.48286356229375116 1101 52-53 0.47830186505028394 1101 54-55 0.49067935455389033 1101 56-57 0.5735744764895117 1101 58-59 0.5285557672055958 1101 60-61 0.3532798470771681 1101 62-63 0.5110199127619168 1101 64-65 0.41920016554603734 1101 66-67 0.42951182827165013 1101 68-69 0.28325029210772357 1101 70-71 0.3328847165989828 1101 72-73 0.4246399821441358 1101 74-75 0.3090937550019248 1101 76-77 0.2539926741008429 1101 78-79 0.4978154401926247 1101 80-81 0.5047356029223593 1101 82-83 0.5096232771349491 1101 84-85 0.5323098941986366 1101 86-87 0.30545460769008415 1101 88-89 0.407562994416395 1101 90-91 0.3696958076089807 1101 92-93 0.49191970241723837 1101 94-95 0.5527755086382093 1101 96-97 0.3668168736932458 1101 98-99 0.45886017203828544 1102 1 0.10639289962244902 1102 2 0.33582061714029265 1102 3 0.23734095037180936 1102 4 0.15988318091535803 1102 5 0.0918439299898921 1102 6 0.1865852207228329 1102 7 0.11621421991865333 1102 8 0.14106110489207424 1102 9 0.4104503984150938 1102 10-11 0.32848119029974043 1102 12-13 0.29151247610260356 1102 14-15 0.5499103386275763 1102 16-17 0.3954934445627387 1102 18-19 0.2643633806063903 1102 20-21 0.3697554143705517 1102 22-23 0.2084616585092789 1102 24-25 0.39047973038172756 1102 26-27 0.25455426903508993 1102 28-29 0.28054872611481585 1102 30-31 0.2380864906745046 1102 32-33 0.2816541918474229 1102 34-35 0.33811608600790777 1102 36-37 0.31754624083382765 1102 38-39 0.22935457347588084 1102 40-41 0.26261134313514845 1102 42-43 0.2317168559233167 1102 44-45 0.33826139897484353 1102 46-47 0.5189934427723841 1102 48-49 0.540747196490436 1102 50-51 0.40784500692958403 1102 52-53 0.5466821161067585 1102 54-55 0.46632286416754454 1102 56-57 0.5545412218455397 1102 58-59 0.45329088981660703 1102 60-61 0.384668316562248 1102 62-63 0.48103157257997964 1102 64-65 0.4049750082511636 1102 66-67 0.41986576793332375 1102 68-69 0.37414766642994834 1102 70-71 0.38618151707949266 1102 72-73 0.47863934583479306 1102 74-75 0.3347510944848082 1102 76-77 0.2996110675306056 1102 78-79 0.5074141669300971 1102 80-81 0.4766185440996722 1102 82-83 0.5308772930739991 1102 84-85 0.5272612291916552 1102 86-87 0.395796394772578 1102 88-89 0.3650045157993205 1102 90-91 0.3142656185610164 1102 92-93 0.40995620046827597 1102 94-95 0.4608973421587734 1102 96-97 0.35110136096250244 1102 98-99 0.3453905195929039 1103 1 0.08321416245948399 1103 2 0.26293064445821557 1103 3 0.2453355747841286 1103 4 0.21286562078037718 1103 5 0.06478475745562662 1103 6 0.17932972790325863 1103 7 0.21287300953321875 1103 8 0.0818305897612106 1103 9 0.4319166867946791 1103 10-11 0.24502410588488033 1103 12-13 0.2856818337915925 1103 14-15 0.48932428281802487 1103 16-17 0.43547994175602156 1103 18-19 0.20753180918127612 1103 20-21 0.302351661547263 1103 22-23 0.19977277910913926 1103 24-25 0.24067374226718385 1103 26-27 0.15256368486424776 1103 28-29 0.07822887797432543 1103 30-31 0.11879450015871384 1103 32-33 0.14915003102478153 1103 34-35 0.21768359647627733 1103 36-37 0.18479835538943234 1103 38-39 0.059022703441975466 1103 40-41 0.021452477215717636 1103 42-43 -0.030848802100287287 1103 44-45 0.1624457908389374 1103 46-47 0.24339752208944532 1103 48-49 0.15219943446622608 1103 50-51 0.15028152370092585 1103 52-53 0.16629182273631926 1103 54-55 0.17709425449879035 1103 56-57 0.24312062590267658 1103 58-59 0.23355967666785205 1103 60-61 0.11791002694919683 1103 62-63 0.16138894570294582 1103 64-65 -0.01745899030572673 1103 66-67 0.1058997167847977 1103 68-69 0.09918749643004787 1103 70-71 0.1042230674861031 1103 72-73 0.17718119878878724 1103 74-75 0.1320035935296957 1103 76-77 0.08672708722963307 1103 78-79 0.32505230301333654 1103 80-81 0.13567924850013924 1103 82-83 0.2170431850006196 1103 84-85 0.27516324425078054 1103 86-87 0.21100767287687816 1103 88-89 0.13979804571443566 1103 90-91 0.056194231727655364 1103 92-93 0.07168345735995629 1103 94-95 0.22590481458934875 1103 96-97 0.16706046778951134 1103 98-99 0.08775674920536503 1104 1 0.1283834322638313 1104 2 0.31784152280100386 1104 3 0.19404962323798713 1104 4 0.1726404345012824 1104 5 0.040299307663836714 1104 6 0.10142964983992897 1104 7 0.0656247601751545 1104 8 0.06391702490272877 1104 9 0.36892048314737025 1104 10-11 0.20523411323030416 1104 12-13 0.2361057315528896 1104 14-15 0.36764127698086924 1104 16-17 0.23539390784680592 1104 18-19 0.1735453283040158 1104 20-21 0.2972189401423435 1104 22-23 0.2249293734889335 1104 24-25 0.2502746910676521 1104 26-27 0.07828640830394562 1104 28-29 0.08516435437854852 1104 30-31 0.132149570474553 1104 32-33 0.17892269082567935 1104 34-35 0.23708935599881187 1104 36-37 0.12002226895019419 1104 38-39 -0.06518857046276594 1104 40-41 -0.024754955141318646 1104 42-43 0.09791921695575923 1104 44-45 0.10346949329876054 1104 46-47 0.24659457812695962 1104 48-49 0.30897872203137666 1104 50-51 0.22269071055578138 1104 52-53 0.23482744512941878 1104 54-55 0.21397995392438673 1104 56-57 0.2716630009463259 1104 58-59 0.2350635459889574 1104 60-61 0.21112355386757287 1104 62-63 0.21525964463278768 1104 64-65 0.08237418282094922 1104 66-67 0.11893863160468499 1104 68-69 0.07697809151139268 1104 70-71 0.13430701051123606 1104 72-73 0.18276973784085015 1104 74-75 0.14884523692006013 1104 76-77 0.103652260441784 1104 78-79 0.27392368825396574 1104 80-81 0.1751174255453094 1104 82-83 0.13980484673964355 1104 84-85 0.22214332782079182 1104 86-87 0.18022930100966406 1104 88-89 0.12922703050332274 1104 90-91 0.1367442256163507 1104 92-93 0.17221807135183909 1104 94-95 0.22610228617236672 1104 96-97 0.19006693009893638 1104 98-99 0.16740396318978412 1105 1 0.13008442970721745 1105 2 0.287847521176019 1105 3 0.20568994552528608 1105 4 0.2023164843980183 1105 5 0.03147393029721002 1105 6 0.10559815258832828 1105 7 0.11722657128149905 1105 8 -0.01588413090053109 1105 9 0.3072262441009457 1105 10-11 0.19410066008257587 1105 12-13 0.18678451082296021 1105 14-15 0.36684652525208605 1105 16-17 0.3050003602332154 1105 18-19 0.17347863322406454 1105 20-21 0.25648932375437994 1105 22-23 0.09067685592155783 1105 24-25 0.24410154006416462 1105 26-27 0.0937797767403481 1105 28-29 0.1461593016432161 1105 30-31 0.12539071630751408 1105 32-33 0.16940923558063048 1105 34-35 0.2156190586019875 1105 36-37 0.08719996424977694 1105 38-39 0.07182165646113248 1105 40-41 -0.03041843352940532 1105 42-43 0.12983284106433501 1105 44-45 0.07058516479671795 1105 46-47 0.21499690680811057 1105 48-49 0.22931367834684835 1105 50-51 0.1260400270329356 1105 52-53 0.03829163252256507 1105 54-55 0.14622675855382283 1105 56-57 0.14105632395578027 1105 58-59 0.14913458389752066 1105 60-61 0.08913850782741406 1105 62-63 0.10652248212431203 1105 64-65 -0.03678284013694366 1105 66-67 0.07039330382362863 1105 68-69 -0.03492362459434162 1105 70-71 -0.045607993346933995 1105 72-73 0.05698785693696351 1105 74-75 0.004656651644417309 1105 76-77 -0.01714612850450692 1105 78-79 0.18032237126821116 1105 80-81 0.06447042968125061 1105 82-83 0.07891075726486463 1105 84-85 0.024327779887006074 1105 86-87 -0.0023092316846877736 1105 88-89 0.04692044272639606 1105 90-91 -3.5874633894650287E-6 1105 92-93 -0.026109346612042117 1105 94-95 0.2215932089570991 1105 96-97 0.08061330407620204 1105 98-99 -0.01848371656813086 1106 1 0.06462183170536662 1106 2 0.22262502468007028 1106 3 0.19557510787090848 1106 4 0.1722380618917967 1106 5 -0.008367025788047044 1106 6 0.13330039204085864 1106 7 0.08468603428501353 1106 8 0.053192088237878465 1106 9 0.3351475413457834 1106 10-11 0.19922694186995216 1106 12-13 0.20744844287914788 1106 14-15 0.356414959602688 1106 16-17 0.2311507877101988 1106 18-19 0.17828881839837862 1106 20-21 0.26803398404186396 1106 22-23 0.05168983158259266 1106 24-25 0.21226311038148538 1106 26-27 0.030258801699240223 1106 28-29 0.15733625777932048 1106 30-31 0.08825635356481598 1106 32-33 0.09511130779807075 1106 34-35 0.20046361310583194 1106 36-37 0.06454203274891057 1106 38-39 -0.007386034953398735 1106 40-41 0.016238206086399032 1106 42-43 0.008395502799082522 1106 44-45 0.024528821272959078 1106 46-47 0.23107510821073163 1106 48-49 0.27957501509728644 1106 50-51 0.11255626578524414 1106 52-53 0.11652298521917004 1106 54-55 0.17467517154776857 1106 56-57 0.17870376389912934 1106 58-59 0.17957985342766847 1106 60-61 0.2028787216099559 1106 62-63 0.25703225391698226 1106 64-65 0.028630788727163292 1106 66-67 0.11387768650636332 1106 68-69 0.02247520648274559 1106 70-71 0.060065991473955194 1106 72-73 0.17563889253352016 1106 74-75 0.0605095351908389 1106 76-77 0.08525410914343468 1106 78-79 0.12689662224092757 1106 80-81 0.1544212860845562 1106 82-83 0.11725879837624831 1106 84-85 0.06800844122990313 1106 86-87 0.08725797023656057 1106 88-89 -0.06035887127422512 1106 90-91 -0.02129814310901068 1106 92-93 -0.10048334357450628 1106 94-95 0.10518808715000816 1106 96-97 0.1068945919884392 1106 98-99 0.017828018980438287 1107 1 0.0793852623985174 1107 2 0.24609369816895565 1107 3 0.14963698773406975 1107 4 0.10199833260579538 1107 5 0.0030926240869320054 1107 6 0.10401279758630011 1107 7 0.10433796082250524 1107 8 0.0016725132307868762 1107 9 0.33066561571178 1107 10-11 0.1790467700104159 1107 12-13 0.16859249200016535 1107 14-15 0.2942651245481258 1107 16-17 0.21334420188701841 1107 18-19 0.11643871440217879 1107 20-21 0.2112387927104038 1107 22-23 0.07303867990727753 1107 24-25 0.13897964662665885 1107 26-27 -0.02287545341287256 1107 28-29 0.031695208907471795 1107 30-31 0.060547928537729945 1107 32-33 -0.007384408445069823 1107 34-35 0.12060678615486609 1107 36-37 0.06684079682796096 1107 38-39 0.10388197382864917 1107 40-41 0.11021014869348278 1107 42-43 0.1198633695132898 1107 44-45 0.06182115762272389 1107 46-47 0.0590576449564395 1107 48-49 0.173354642344826 1107 50-51 0.035932487966789495 1107 52-53 0.06056109948723787 1107 54-55 0.07145925339430192 1107 56-57 0.1452738480375615 1107 58-59 0.11447825917658605 1107 60-61 0.13749516036426712 1107 62-63 0.21184420304953022 1107 64-65 0.05756242135812073 1107 66-67 0.003046740002424997 1107 68-69 -0.00946978022903977 1107 70-71 0.01393337054402366 1107 72-73 0.09727822901942318 1107 74-75 0.05978929140059819 1107 76-77 0.08328880690390861 1107 78-79 0.10253297497836655 1107 80-81 0.03542209151908082 1107 82-83 -0.09662257779886119 1107 84-85 -0.06790420810130726 1107 86-87 0.08362407797202209 1107 88-89 0.03248481247524282 1107 90-91 -0.09813540030183177 1107 92-93 -0.1031075843893774 1107 94-95 0.1828835964114468 1107 96-97 0.07093546781806737 1107 98-99 -8.528309986708393E-4 1108 1 0.09582673607224734 1108 2 0.22020340847705455 1108 3 0.1493134043128066 1108 4 0.04174499546925858 1108 5 -0.07580336997378367 1108 6 -0.03712586073427815 1108 7 0.04531489292964608 1108 8 -0.04117494500874841 1108 9 0.21902672913348908 1108 10-11 0.14031118457795344 1108 12-13 0.1390240368999045 1108 14-15 0.20067162872499011 1108 16-17 0.17801447046262808 1108 18-19 -0.02180478380068962 1108 20-21 0.1747351264504715 1108 22-23 -0.008510735049966911 1108 24-25 0.06291738859784601 1108 26-27 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84-85 -0.03128840700385993 2215 86-87 -0.21852814771526852 2215 88-89 -0.09413534556585645 2215 90-91 -0.26322802572591186 2215 92-93 -0.12752458629666208 2215 94-95 -0.1827561143390426 2215 96-97 -0.11912044801567845 2215 98-99 -0.03527633186109469 2216 1 -0.19781731149675608 2216 2 0.003099103124682756 2216 3 -0.1714762579325253 2216 4 -0.31943915990120786 2216 5 -0.23065363525247307 2216 6 -0.22658238631984773 2216 7 -0.2838390646108877 2216 8 -0.21572897919622847 2216 9 0.0756634988930287 2216 10-11 -0.1759104890816019 2216 12-13 -0.1006987192455071 2216 14-15 -0.4591946343746187 2216 16-17 -0.5980136461074679 2216 18-19 -0.586576668025586 2216 20-21 -0.8672779986288788 2216 22-23 -0.7232721137264235 2216 24-25 -0.5979794263666136 2216 26-27 -0.680136871367246 2216 28-29 -0.6866490939340935 2216 30-31 -0.3846718710240751 2216 32-33 -0.45954992595738275 2216 34-35 -0.4820063994408841 2216 36-37 -0.42662546038067006 2216 38-39 -0.4090316103259184 2216 40-41 -0.44197555930053767 2216 42-43 -0.5470659918820004 2216 44-45 -0.39988058586340713 2216 46-47 -0.718611734297177 2216 48-49 -0.8417939830248997 2216 50-51 -0.44442249626962393 2216 52-53 -0.41261632806813253 2216 54-55 -0.31204731384490714 2216 56-57 -0.4846945344408624 2216 58-59 -0.39505434719197297 2216 60-61 -0.4534174688236021 2216 62-63 -0.4487506588037462 2216 64-65 -0.47263397214207004 2216 66-67 -0.43090013683085004 2216 68-69 -0.3778110071992131 2216 70-71 -0.3400984592177565 2216 72-73 -0.28873885328409443 2216 74-75 -0.3995631379337965 2216 76-77 -0.31710185477592745 2216 78-79 -0.27252084642639574 2216 80-81 -0.14618698664632745 2216 82-83 -0.1642521421559877 2216 84-85 -0.19861380706351284 2216 86-87 -0.3203357460718905 2216 88-89 -0.1360371657171342 2216 90-91 -0.22595877805629883 2216 92-93 -0.16917181063971753 2216 94-95 -0.24304870667923595 2216 96-97 -0.2655764065935955 2216 98-99 -0.1821235315877665 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 8.0 9 44.0 10 115.0 11 234.0 12 346.0 13 708.0 14 1701.0 15 4214.0 16 9394.0 17 17369.0 18 28579.0 19 40437.0 20 52736.0 21 67427.0 22 79970.0 23 78051.0 24 65581.0 25 62164.0 26 73009.0 27 93129.0 28 122203.0 29 159832.0 30 209458.0 31 273639.0 32 358399.0 33 468885.0 34 620716.0 35 870485.0 36 1407193.0 37 1921701.0 38 692062.0 39 991.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02971158338202 20.00022374923215 13.444782740138542 24.525281927247295 2 15.543404047041015 21.742061545706846 37.94805313656476 24.766481270687386 3 17.410619500872663 27.33973971761186 30.438696377484003 24.810944404031474 4 12.50544289904097 17.89622634234614 37.906045409329145 31.69228534928375 5 13.235681015222866 38.036528559440875 33.66744222840484 15.060348196931416 6 29.741594030418543 38.7282765995183 17.697968584126528 13.832160785936628 7 25.681808250586702 33.00458308807086 23.268515495875736 18.045093165466703 8 26.852166492305397 32.368708535648096 20.567385274998138 20.211739697048365 9 27.02873887417892 13.645994434502843 20.74195832444887 38.58330836686937 10-11 25.34635345042528 25.3992530311871 27.229467996781814 22.024925521605805 12-13 26.240010641606627 23.583419657155194 27.51389963474099 22.66267006649719 14-15 23.95836352334334 24.14685200585586 25.981785551632612 25.91299891916819 16-17 22.675637709715794 26.46805151970509 26.767019168130645 24.089291602448473 18-19 23.029850477715602 25.05745567925067 29.86545693362362 22.047236909410113 20-21 24.69555108870833 24.30056498191698 28.652088865126636 22.351795064248055 22-23 23.668205501247947 24.200960572076323 28.730827757628415 23.40000616904732 24-25 23.048055593398093 24.941747485470607 28.880979798940466 23.129217122190834 26-27 23.444114857379336 24.722296479273286 28.668713162433583 23.16487550091379 28-29 22.670072923280184 24.65055559982418 29.515279959078654 23.164091517816978 30-31 23.382752114826534 24.579592277380932 29.015644961045034 23.0220106467475 32-33 23.083752528666793 24.693469035238113 28.326639488585975 23.89613894750912 34-35 23.60024316328183 24.655863550955047 29.0742187801223 22.66967450564082 36-37 23.584126527160514 24.633327249967227 28.382321566732383 23.400224656139873 38-39 23.642995946421824 24.070812951914846 28.58758376409563 23.698607337567697 40-41 23.370561820280226 24.582496870493703 28.839004572806324 23.207936736419743 42-43 24.195504820853436 24.385415086919306 28.163262552083467 23.255817540143788 44-45 23.632637651636493 24.929590543312894 27.572585503623532 23.865186301427084 46-47 23.408674965748936 24.763764044221787 27.72216924267233 24.10539174735695 48-49 24.36256390747457 25.208179642657935 26.769713319230203 23.65954313063729 50-51 23.509815211328426 25.71907957813998 26.311764373237644 24.459340837293947 52-53 23.51532499649617 25.38550658588544 26.23071230046235 24.868456117156036 54-55 23.923526947169822 25.413576787931287 26.34192201810101 24.32097424679788 56-57 24.16414820233731 24.945067606701993 25.89147339625783 24.99931079470287 58-59 23.45527055128149 25.039706816026158 26.836930230645255 24.668092402047094 60-61 23.641344441045756 25.31738463238904 26.492903025146582 24.548367901418626 62-63 23.2467824562576 25.182301774372235 26.17753618531818 25.393379584051985 64-65 23.248973753274093 24.94253146856742 26.409216042607554 25.399278735550933 66-67 23.98490254190454 25.013282730009074 26.262097116227423 24.739717611858968 68-69 23.943283731714278 24.49851940580876 26.319630982461646 25.23856588001532 70-71 23.098436200420256 24.684907085881903 26.574985825614345 25.641670888083496 72-73 23.868146895298416 24.732385442076502 26.43534452844059 24.96412313418449 74-75 24.143254275278313 24.663517025285383 26.34162641791697 24.851602281519334 76-77 23.480872097656018 25.09099987404862 26.291586447631214 25.13654158066415 78-79 23.534495583165747 25.34426502355013 26.134626611791866 24.986612781492255 80-81 23.08294092040736 25.125730862246836 26.596098529349582 25.195229687996218 82-83 23.42231113076067 25.215980917080294 26.027737579008786 25.33397037315025 84-85 23.49077470382147 25.425073064654185 25.521149550559198 25.56300268096515 86-87 23.057926069108753 25.113086348669412 25.933794555301652 25.895193026920182 88-89 23.13849639753341 24.741716126146738 26.086131467539243 26.03365600878061 90-91 23.467616310030962 24.975762283961092 25.806942278217427 25.74967912779052 92-93 23.172385659818502 25.17591452127711 25.648475043333203 26.003224775571184 94-95 22.342680831752613 25.354031827617135 26.331864618820322 25.97142272180993 96-97 23.100414464840277 25.19216479785738 25.759204776553542 25.948215960748804 98-99 22.600581175666193 25.286487987065563 25.878028937972804 26.234901899295444 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 180.0 1 169.5 2 428.0 3 723.0 4 515.0 5 406.5 6 904.0 7 2462.5 8 4364.5 9 4290.5 10 2630.5 11 1650.0 12 1634.0 13 1898.5 14 2298.5 15 2919.5 16 3693.5 17 4599.5 18 5755.5 19 7126.5 20 8695.5 21 10732.0 22 13366.5 23 16913.0 24 21069.0 25 26316.0 26 33235.5 27 40011.0 28 49163.0 29 60757.5 30 70045.5 31 81326.0 32 94819.5 33 107071.0 34 118452.0 35 131241.0 36 140453.0 37 151260.0 38 165798.0 39 162991.5 40 159535.0 41 167925.5 42 179260.5 43 201224.0 44 227626.5 45 258354.5 46 299913.5 47 334014.5 48 347018.5 49 357188.5 50 378886.0 51 365296.0 52 335334.0 53 325996.5 54 303583.0 55 289545.0 56 267256.0 57 242293.0 58 236005.5 59 210795.0 60 167788.5 61 119796.5 62 94295.0 63 75983.5 64 54062.0 65 43568.5 66 32290.0 67 23629.5 68 17942.0 69 15051.5 70 14845.0 71 14881.0 72 17069.0 73 14961.5 74 9081.5 75 6774.0 76 5735.0 77 4151.0 78 2071.0 79 1412.0 80 1372.0 81 1172.5 82 1114.0 83 935.5 84 572.5 85 431.0 86 218.0 87 66.5 88 55.0 89 50.0 90 35.5 91 19.5 92 10.0 93 6.0 94 3.5 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.054197651135233234 2 0.00868807497448842 3 0.0 4 0.0 5 0.0023519492904310363 6 0.0 7 0.0 8 0.0 9 0.004408298396818828 10-11 0.0 12-13 0.0 14-15 0.0025511581101123537 16-17 0.004363315760116595 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 5.462177313842571E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.4780009202162251E-4 54-55 0.0 56-57 0.0010217484622364339 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0015422618297908435 70-71 2.506175473410121E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.8195682180963864E-4 80-81 9.382092797894299E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 4.4340027606486757E-4 92-93 0.0010731571898961287 94-95 6.811656414909559E-4 96-97 3.020262750007069E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7780780.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.15678021656862 #Duplication Level Percentage of deduplicated Percentage of total 1 72.51458185493476 24.04350053060447 2 15.703110749034904 10.413291836443731 3 5.22747237269614 5.199784576490112 4 2.2735737477498894 3.015375402412132 5 1.1202539378384417 1.8572006801827368 6 0.6621185298422382 1.3172231142777973 7 0.4358839217264515 1.0116755174833967 8 0.28392822043094623 0.7531316481688266 9 0.1996856025565959 0.595883847274391 >10 1.198579576520812 7.687743429781781 >50 0.14939752485116894 3.5022426809293057 >100 0.16909394587732257 12.311694106255363 >500 0.03417171199059909 7.877893870166084 >1k 0.026534205401666275 16.32484698514297 >5k 0.0014172572617943042 2.9177269325833826 >10k+ 1.9684128636032004E-4 1.1707848418038311 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23650 0.3039541022879454 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 19863 0.25528288937612936 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 18473 0.23741835651438548 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15457 0.19865617585897558 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12250 0.1574392284578153 No Hit GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA 8853 0.11378036649281949 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA 8257 0.10612046607152498 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT 8041 0.10334439477790146 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 8012 0.10297168150236866 No Hit TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT 7933 0.10195635913108968 No Hit GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA 7926 0.10186639385768523 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA 7816 0.10045265384704362 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2852181914923697E-5 2 0.0 0.0 0.0 0.0 1.2852181914923697E-5 3 0.0 0.0 0.0 0.0 1.2852181914923697E-5 4 0.0 0.0 0.0 0.0 1.2852181914923697E-5 5 0.0 0.0 0.0 1.2852181914923697E-5 1.2852181914923697E-5 6 0.0 0.0 0.0 2.5704363829847394E-5 2.5704363829847394E-5 7 0.0 0.0 0.0 2.5704363829847394E-5 2.5704363829847394E-5 8 1.2852181914923697E-5 0.0 0.0 3.855654574477109E-5 2.5704363829847394E-5 9 1.2852181914923697E-5 0.0 0.0 3.855654574477109E-5 3.855654574477109E-5 10-11 1.2852181914923697E-5 0.0 0.0 5.1408727659694794E-5 3.855654574477109E-5 12-13 1.2852181914923697E-5 0.0 0.0 7.068700053208034E-5 3.855654574477109E-5 14-15 1.2852181914923697E-5 0.0 0.0 7.711309148954218E-5 3.855654574477109E-5 16-17 1.2852181914923697E-5 0.0 0.0 1.863566377663936E-4 3.855654574477109E-5 18-19 1.2852181914923697E-5 0.0 0.0 2.506175473410121E-4 5.140872765969479E-5 20-21 1.2852181914923697E-5 0.0 0.0 3.919915484051728E-4 5.140872765969479E-5 22-23 1.2852181914923697E-5 0.0 0.0 0.0014522965563863778 5.140872765969479E-5 24-25 2.5704363829847394E-5 0.0 0.0 0.0037142805734129486 6.426090957461849E-5 26-27 3.855654574477109E-5 0.0 0.0 0.005995542863311904 8.996527340446588E-5 28-29 3.855654574477109E-5 0.0 0.0 0.015422618297908437 8.996527340446588E-5 30-31 3.855654574477109E-5 0.0 0.0 0.046184315711278304 8.996527340446588E-5 32-33 3.855654574477109E-5 0.0 0.0 0.10125591521672635 8.996527340446588E-5 34-35 3.855654574477109E-5 0.0 0.0 0.1740121170371094 1.1566963723431327E-4 36-37 3.855654574477109E-5 0.0 0.0 0.2876703878017371 1.2852181914923698E-4 38-39 3.855654574477109E-5 0.0 0.0 0.46429792385853347 1.5422618297908437E-4 40-41 3.855654574477109E-5 0.0 0.0 0.6761597166350931 1.5422618297908437E-4 42-43 3.855654574477109E-5 6.426090957461848E-6 0.0 0.8688589061765015 1.5422618297908437E-4 44-45 3.855654574477109E-5 1.2852181914923697E-5 0.0 1.0960212215227778 1.5422618297908437E-4 46-47 3.855654574477109E-5 1.2852181914923697E-5 0.0 1.3407203390919675 1.5422618297908437E-4 48-49 3.855654574477109E-5 1.2852181914923697E-5 0.0 1.606606278547909 1.606522739365462E-4 50-51 3.855654574477109E-5 1.2852181914923697E-5 0.0 1.9040186202411584 1.6707836489400805E-4 52-53 3.855654574477109E-5 1.2852181914923697E-5 0.0 2.197067646174291 1.6707836489400805E-4 54-55 3.855654574477109E-5 1.2852181914923697E-5 0.0 2.480374718215912 1.7993054680893176E-4 56-57 3.855654574477109E-5 1.2852181914923697E-5 0.0 2.8030223190991137 1.9278272872385544E-4 58-59 3.855654574477109E-5 1.2852181914923697E-5 0.0 3.1591177234159042 2.1206100159624098E-4 60-61 3.855654574477109E-5 1.2852181914923697E-5 0.0 3.5189209822151506 2.1848709255370283E-4 62-63 4.498263670223294E-5 1.2852181914923697E-5 0.0 3.8820593822213194 2.3133927446862654E-4 64-65 5.140872765969479E-5 1.2852181914923697E-5 0.0 4.264251141916363 2.3133927446862654E-4 66-67 5.140872765969479E-5 1.2852181914923697E-5 0.0 4.675276771737538 2.3133927446862654E-4 68-69 5.140872765969479E-5 1.2852181914923697E-5 0.0 5.114557923498673 2.377653654260884E-4 70-71 5.140872765969479E-5 1.2852181914923697E-5 0.0 5.614353573806225 2.4419145638355025E-4 72-73 5.140872765969479E-5 1.2852181914923697E-5 0.0 6.102473016844069 2.4419145638355025E-4 74-75 5.140872765969479E-5 1.2852181914923697E-5 0.0 6.5755489809504954 2.4419145638355025E-4 76-77 5.140872765969479E-5 1.2852181914923697E-5 0.0 7.0830109577703 2.4419145638355025E-4 78-79 5.140872765969479E-5 1.2852181914923697E-5 0.0 7.610001825009832 2.698958202133976E-4 80-81 5.140872765969479E-5 1.2852181914923697E-5 0.0 8.185998061890967 2.891740930857832E-4 82-83 5.140872765969479E-5 1.2852181914923697E-5 0.0 8.802375854348792 3.0845236595816874E-4 84-85 5.140872765969479E-5 1.2852181914923697E-5 0.0 9.396538650366672 3.0845236595816874E-4 86-87 5.140872765969479E-5 1.2852181914923697E-5 0.0 10.004915959582458 3.213045478730924E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 56710 0.0 29.989328 1 TATCACG 480 0.0 29.064827 2 GTACTAG 3375 0.0 24.540604 1 CAACGCA 69455 0.0 24.330265 5 ATCAACG 69460 0.0 24.288342 3 AACGCAG 72955 0.0 23.411007 6 TATCAAC 73630 0.0 23.116072 2 TCAACGC 73220 0.0 23.098246 4 AGTACTT 36180 0.0 22.493946 12-13 ACGCAGA 77205 0.0 21.694643 7 CGCAGAG 77670 0.0 21.54123 8 GGTATCA 38555 0.0 21.144257 1 GCAGAGT 80625 0.0 20.671503 9 TAGGCAT 5110 0.0 20.293146 5 GAGTACT 35060 0.0 20.208155 12-13 TAACGCA 660 0.0 19.023273 4 TACTTTT 43435 0.0 18.956059 14-15 TCTAACG 1615 0.0 18.71667 2 CTAGGCA 5595 0.0 18.700268 4 ACAACGC 780 0.0 18.481298 3 >>END_MODULE