FastQCFastQC Report
Fri 27 May 2016
ERR765524_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765524_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8130445
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA163450.20103450672134182No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT148820.18304041168718319No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG31250.022.023011
GTACATA22700.020.485151
GTACAAG37950.020.4630181
GTATCAA400350.020.4078331
GTATAAG37600.019.9114571
CTAACGC14300.019.5110323
TAGTACT38100.019.0374374
TACATGA29350.019.0124512
CATGGGG213100.018.7639354
GTACATG594350.018.6365051
TCTAACG14550.018.5365962
TACATGG584900.017.943822
GTGATCG23050.017.7504758
GTTATAT26350.017.4710731
TATATAC41050.017.4450653
GTACAAA39300.017.2752881
CTCGTCG26550.017.1632619
TGATCGC23850.017.1566549
ACGTTAA8450.017.057028
ATTTAGG34700.017.0192031
Produced by FastQC (version 0.11.5)