##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765524_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8130445 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.130641557749914 33.0 33.0 33.0 27.0 33.0 2 31.07906615197569 33.0 33.0 33.0 27.0 33.0 3 31.07028741969228 33.0 33.0 33.0 27.0 33.0 4 34.59573221391941 37.0 37.0 37.0 27.0 37.0 5 34.697811866386154 37.0 37.0 37.0 27.0 37.0 6 34.66371520870014 37.0 37.0 37.0 27.0 37.0 7 34.56750559163736 37.0 37.0 37.0 27.0 37.0 8 34.504890814709405 37.0 37.0 37.0 27.0 37.0 9 34.370028085793585 37.0 37.0 37.0 27.0 37.0 10-11 34.39635173720504 37.0 37.0 37.0 27.0 37.0 12-13 34.31067383150615 37.0 37.0 37.0 27.0 37.0 14-15 35.68246178407209 37.0 37.0 40.0 27.0 40.0 16-17 35.759777785840754 37.0 37.0 40.0 27.0 40.0 18-19 35.77908767601281 37.0 37.0 40.0 27.0 40.0 20-21 35.790339028183574 37.0 37.0 40.0 27.0 40.0 22-23 35.79886402281794 37.0 37.0 40.0 27.0 40.0 24-25 35.880613299764036 37.0 37.0 40.0 27.0 40.0 26-27 35.84491291682066 37.0 37.0 40.0 27.0 40.0 28-29 35.849193125345536 37.0 37.0 40.0 27.0 40.0 30-31 35.76022425586791 37.0 37.0 40.0 27.0 40.0 32-33 35.66636469467538 37.0 35.0 40.0 27.0 40.0 34-35 35.515096221670525 37.0 33.0 40.0 27.0 40.0 36-37 35.301457484799414 37.0 33.0 40.0 27.0 40.0 38-39 34.999931246075704 37.0 33.0 40.0 27.0 40.0 40-41 35.09937432698948 37.0 33.0 40.0 27.0 40.0 42-43 35.0099759607254 37.0 33.0 40.0 27.0 40.0 44-45 34.919195136305575 37.0 33.0 40.0 27.0 40.0 46-47 34.82697472278578 37.0 33.0 40.0 27.0 40.0 48-49 34.69304134029564 37.0 33.0 40.0 27.0 40.0 50-51 34.510379382678316 37.0 33.0 40.0 27.0 40.0 52-53 34.3665260019593 37.0 33.0 40.0 27.0 40.0 54-55 34.167226640116255 37.0 33.0 40.0 24.5 40.0 56-57 33.965546166292256 37.0 33.0 38.5 22.0 40.0 58-59 33.75301493337696 37.0 33.0 37.0 22.0 40.0 60-61 33.537628198702535 37.0 33.0 37.0 22.0 40.0 62-63 33.30770732721272 37.0 33.0 37.0 22.0 40.0 64-65 33.0895086308314 37.0 33.0 37.0 22.0 40.0 66-67 32.88298709357237 37.0 33.0 37.0 22.0 40.0 68-69 32.69476012690572 37.0 33.0 37.0 22.0 40.0 70-71 32.450960064301526 35.0 33.0 37.0 22.0 37.0 72-73 32.12126636364922 33.0 33.0 37.0 22.0 37.0 74-75 31.89170666550232 33.0 33.0 37.0 22.0 37.0 76-77 31.71043036389767 33.0 33.0 37.0 22.0 37.0 78-79 31.468704480505068 33.0 27.0 37.0 22.0 37.0 80-81 31.266188812543472 33.0 27.0 37.0 22.0 37.0 82-83 31.146060885966268 33.0 27.0 37.0 22.0 37.0 84-85 31.013916827430727 33.0 27.0 37.0 22.0 37.0 86-87 30.75006238895903 33.0 27.0 37.0 15.0 37.0 88-89 30.609591295433397 33.0 27.0 37.0 15.0 37.0 90-91 30.46891670751109 33.0 27.0 37.0 15.0 37.0 92-93 30.220278840826055 33.0 27.0 37.0 15.0 37.0 94-95 30.070705723979437 33.0 27.0 37.0 15.0 37.0 96-97 29.887093203385547 33.0 27.0 37.0 15.0 37.0 98-99 28.8411764054735 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3620715067951714 1101 2 0.3510499108432654 1101 3 0.3380725092311785 1101 4 0.3770313175485924 1101 5 0.3875999654063449 1101 6 0.43903427828095687 1101 7 0.3854788061841177 1101 8 0.5224410043917658 1101 9 0.4213017384946056 1101 10-11 0.383868869350394 1101 12-13 0.5224283395566971 1101 14-15 0.6251669390583388 1101 16-17 0.4747759933792963 1101 18-19 0.4375592529625365 1101 20-21 0.5188225249041452 1101 22-23 0.46871118957351854 1101 24-25 0.3364968232519061 1101 26-27 0.4386580711253387 1101 28-29 0.4108634770352353 1101 30-31 0.6245214404478503 1101 32-33 0.772239159438314 1101 34-35 0.806061746528627 1101 36-37 0.8878892086679713 1101 38-39 0.9033165247446604 1101 40-41 0.6830760517300618 1101 42-43 0.592698454862834 1101 44-45 0.5127213930549814 1101 46-47 0.5486653572789777 1101 48-49 0.40832818443882957 1101 50-51 0.392476243066767 1101 52-53 0.5223107580861566 1101 54-55 0.36219659096876455 1101 56-57 0.44957249040183456 1101 58-59 0.4434093176395635 1101 60-61 0.23312012085908407 1101 62-63 0.37573380478236373 1101 64-65 0.4564886282498435 1101 66-67 0.4704008347597508 1101 68-69 0.5341987881419783 1101 70-71 0.6199930448571749 1101 72-73 0.398840596275285 1101 74-75 0.4290574954650488 1101 76-77 0.38864788032085684 1101 78-79 0.39521258703324236 1101 80-81 0.37854840657548294 1101 82-83 0.4676749699004503 1101 84-85 0.4183854415532693 1101 86-87 0.3510668213271373 1101 88-89 0.28416250214160144 1101 90-91 0.2727910726893441 1101 92-93 0.3413913569785194 1101 94-95 0.3626752396768005 1101 96-97 0.28310367009483883 1101 98-99 0.27475624806723076 1102 1 0.3170618815827595 1102 2 0.33133856379317805 1102 3 0.29172565988295673 1102 4 0.3447416798863969 1102 5 0.27148673522023614 1102 6 0.36306594868671027 1102 7 0.3524939780987353 1102 8 0.41825051289150394 1102 9 0.29859377808277543 1102 10-11 0.3364864006493349 1102 12-13 0.43163494745495967 1102 14-15 0.533395888980607 1102 16-17 0.40106646770490784 1102 18-19 0.364518604019878 1102 20-21 0.40335925816592777 1102 22-23 0.4091210715831295 1102 24-25 0.29632848973248826 1102 26-27 0.44274431765406774 1102 28-29 0.3850769551991249 1102 30-31 0.5016493782875173 1102 32-33 0.6166418127867743 1102 34-35 0.6035214970654721 1102 36-37 0.751104950236865 1102 38-39 0.726977433710978 1102 40-41 0.5674937575279628 1102 42-43 0.4141380810956292 1102 44-45 0.28953851581070467 1102 46-47 0.2733390247516141 1102 48-49 0.2515527071461321 1102 50-51 0.28651672840646825 1102 52-53 0.31573986185546943 1102 54-55 0.2167828261373117 1102 56-57 0.30721982732596587 1102 58-59 0.1505028536614148 1102 60-61 0.1630002916674158 1102 62-63 0.23542860138577026 1102 64-65 0.2964892911309178 1102 66-67 0.2577588558528916 1102 68-69 0.3626548762201409 1102 70-71 0.3458687339705122 1102 72-73 0.20754421261730016 1102 74-75 0.26692282009884494 1102 76-77 0.2915409531240094 1102 78-79 0.28509722943862315 1102 80-81 0.22026057913238262 1102 82-83 0.23945928615042078 1102 84-85 0.306084856581073 1102 86-87 0.2892919238517955 1102 88-89 0.17318893138934754 1102 90-91 0.19424751321828282 1102 92-93 0.27207895602895604 1102 94-95 0.21824929184033337 1102 96-97 0.07280679587228889 1102 98-99 0.14566755848784396 1103 1 0.10616850045378001 1103 2 0.1672159115417564 1103 3 0.05571804751715348 1103 4 0.111160757978503 1103 5 0.09811536217868877 1103 6 0.2530175916427453 1103 7 0.1751052557916566 1103 8 0.157832572813291 1103 9 0.07134080199075044 1103 10-11 0.14195805753743684 1103 12-13 0.1953912794880992 1103 14-15 0.30926004000444607 1103 16-17 0.09941310652352087 1103 18-19 0.09639049074965556 1103 20-21 0.15006494522900482 1103 22-23 0.09995507247821678 1103 24-25 0.09502671163353682 1103 26-27 0.24380305199451868 1103 28-29 0.12186419121058378 1103 30-31 0.36313148570531695 1103 32-33 0.4379062328999126 1103 34-35 0.49624713687653355 1103 36-37 0.5988535083554254 1103 38-39 0.607220903797355 1103 40-41 0.3748904389609251 1103 42-43 0.3172665233287617 1103 44-45 0.2072268862866835 1103 46-47 0.16797308175976156 1103 48-49 0.06362031993222672 1103 50-51 0.061002094182548205 1103 52-53 0.17640689874365734 1103 54-55 0.029594612333475823 1103 56-57 0.1065100316285168 1103 58-59 0.0641979161444155 1103 60-61 -0.0072879532362293276 1103 62-63 0.051450073429570864 1103 64-65 0.13099779122732969 1103 66-67 0.17563872865568442 1103 68-69 0.23573643344707307 1103 70-71 0.23223824742482435 1103 72-73 0.017395443292151924 1103 74-75 0.07178397680614168 1103 76-77 0.10041072473795509 1103 78-79 0.0685923042623493 1103 80-81 -0.010099536205395054 1103 82-83 0.0786764000239728 1103 84-85 0.20359612737297894 1103 86-87 0.08553713491107828 1103 88-89 0.11319230849603201 1103 90-91 -0.01639306754486114 1103 92-93 -0.06914157711483426 1103 94-95 0.07058893532900612 1103 96-97 -0.07997322219368641 1103 98-99 -0.029049013074526897 1104 1 0.1624405036183738 1104 2 0.16408718544300172 1104 3 0.12549069949927372 1104 4 0.23600262588167453 1104 5 0.04799018413212508 1104 6 0.21602987424815012 1104 7 0.1572920711388619 1104 8 0.22435953474550985 1104 9 0.15946737176530235 1104 10-11 0.1791445653113044 1104 12-13 0.22670402039434379 1104 14-15 0.26930869618703923 1104 16-17 0.12297595269665607 1104 18-19 0.20145068474003835 1104 20-21 0.20543892362364602 1104 22-23 0.061101371510090985 1104 24-25 0.04421496300723504 1104 26-27 0.17785807908381912 1104 28-29 0.10816025594705536 1104 30-31 0.2977387244787124 1104 32-33 0.39470092534279644 1104 34-35 0.47832653330969777 1104 36-37 0.5106955985457304 1104 38-39 0.5286845039655148 1104 40-41 0.33860323061511366 1104 42-43 0.2517692898003361 1104 44-45 0.16596029396293233 1104 46-47 0.14057941125496853 1104 48-49 0.006006164698476368 1104 50-51 -0.021521965899466977 1104 52-53 0.1494957608209475 1104 54-55 -0.07883345347914172 1104 56-57 0.1182114889567103 1104 58-59 0.11231067392250083 1104 60-61 -0.08065305855377858 1104 62-63 0.03028210785871721 1104 64-65 0.09066055708706955 1104 66-67 0.07575295788583247 1104 68-69 0.13857006460895605 1104 70-71 0.2333117810267069 1104 72-73 -0.022531831007441383 1104 74-75 0.017363505571996285 1104 76-77 0.05544255841341439 1104 78-79 0.05494900630536037 1104 80-81 -0.058023909449389066 1104 82-83 0.040837816898154244 1104 84-85 0.14160051206439306 1104 86-87 0.017317397231078502 1104 88-89 -0.007285788938041549 1104 90-91 -0.12746686336211255 1104 92-93 -0.03750549021321703 1104 94-95 -0.010178175764036013 1104 96-97 -0.10834272789584531 1104 98-99 -0.12495541383802333 1105 1 0.059430784623568655 1105 2 0.09205019547581017 1105 3 0.05409390278576254 1105 4 -0.0349655467230221 1105 5 -0.11499273496145435 1105 6 0.11887434341251435 1105 7 -0.009617801918210489 1105 8 0.1053287049265208 1105 9 -0.015091633224365353 1105 10-11 0.04541155389643592 1105 12-13 0.061242916559343996 1105 14-15 0.16127850070513716 1105 16-17 0.012172575562566124 1105 18-19 0.10367672987788268 1105 20-21 0.06394055995369996 1105 22-23 0.07115664427798407 1105 24-25 -0.09667614587898754 1105 26-27 0.07439281267421194 1105 28-29 0.02029515011805927 1105 30-31 0.2230621421068335 1105 32-33 0.3175422453957495 1105 34-35 0.33325389416864937 1105 36-37 0.4115111260100477 1105 38-39 0.3861984213196905 1105 40-41 0.20812570341297487 1105 42-43 0.07660558514663052 1105 44-45 -0.006059187054773929 1105 46-47 -0.026862047746767814 1105 48-49 -0.15855310947846135 1105 50-51 -0.155962372505968 1105 52-53 -0.06815250125861638 1105 54-55 -0.15198753130556497 1105 56-57 0.06414881724268184 1105 58-59 -0.14277948437544552 1105 60-61 -0.18168093053219536 1105 62-63 -0.10210520609569329 1105 64-65 -0.040881303422445114 1105 66-67 -0.05910057329678153 1105 68-69 0.04791561215160556 1105 70-71 0.08172467611969125 1105 72-73 -0.10985779022391284 1105 74-75 -0.06703298111160194 1105 76-77 -0.017446038878482284 1105 78-79 -0.022528209715265035 1105 80-81 -0.14025980197271792 1105 82-83 -0.04112836491170313 1105 84-85 0.006771400105044734 1105 86-87 -0.0768106308458485 1105 88-89 -0.13035053768628302 1105 90-91 -0.12528682281076087 1105 92-93 -0.08744812139656233 1105 94-95 -0.050376673165214214 1105 96-97 -0.20478771953213126 1105 98-99 -0.09134500956338698 1106 1 0.06136975349988205 1106 2 0.11878536996009004 1106 3 0.014929412872053405 1106 4 -0.07794595757038536 1106 5 -0.023099744561200453 1106 6 0.08786800201448841 1106 7 0.05946923264502857 1106 8 0.08474888449276818 1106 9 0.05976520863791279 1106 10-11 0.043161333976925675 1106 12-13 0.026414070694350755 1106 14-15 0.15357467757699794 1106 16-17 -0.06581270233440506 1106 18-19 0.15385996377544586 1106 20-21 0.06671896336343508 1106 22-23 -0.024147763877643058 1106 24-25 0.07506481501567919 1106 26-27 0.1656630157340473 1106 28-29 0.08722222821262449 1106 30-31 0.2700707061264467 1106 32-33 0.2818698126063168 1106 34-35 0.3785424185371795 1106 36-37 0.44510857237449386 1106 38-39 0.4597754223609769 1106 40-41 0.20675735280511987 1106 42-43 0.042908899554028324 1106 44-45 0.06722034589932946 1106 46-47 0.05948906304764279 1106 48-49 -0.006576842665481308 1106 50-51 -0.06568859742388611 1106 52-53 0.030089520952834903 1106 54-55 -0.10704014482274005 1106 56-57 -0.10192476523977234 1106 58-59 -0.05348623760054494 1106 60-61 -0.14583395145112377 1106 62-63 -0.01822732552353301 1106 64-65 0.1487456036927881 1106 66-67 0.06746631816670146 1106 68-69 0.09374824885462374 1106 70-71 0.07234052848086492 1106 72-73 -0.11980479592501325 1106 74-75 0.007881702487967601 1106 76-77 -0.021102622746642652 1106 78-79 0.019090035350213697 1106 80-81 0.02350639308973257 1106 82-83 0.024769860250049902 1106 84-85 -0.006821583200810721 1106 86-87 -0.05012843905580411 1106 88-89 -0.12970518610391935 1106 90-91 -0.08741816090972421 1106 92-93 -0.12639082954476066 1106 94-95 -0.06321670901412446 1106 96-97 -0.2123849386917449 1106 98-99 -0.1869149146643636 1107 1 0.1173302955718647 1107 2 0.1911587257369689 1107 3 0.0916526611204489 1107 4 0.0317451319486608 1107 5 -0.010412203187740943 1107 6 0.09796253548193334 1107 7 0.08729794942280478 1107 8 0.11830967059650987 1107 9 -0.023861167566295194 1107 10-11 0.01111748520384026 1107 12-13 0.10925740920152549 1107 14-15 0.17626020868237902 1107 16-17 0.0651120481868972 1107 18-19 0.2686583447033968 1107 20-21 0.1859029164496988 1107 22-23 0.04114425484180373 1107 24-25 0.04328781445292407 1107 26-27 0.17112911408970177 1107 28-29 0.10997705181311801 1107 30-31 0.23548720962278225 1107 32-33 0.2862239871451351 1107 34-35 0.36283165875978085 1107 36-37 0.4375151164765114 1107 38-39 0.3902130560134651 1107 40-41 0.2584984713478704 1107 42-43 0.10217499878439895 1107 44-45 0.15551948623630096 1107 46-47 0.02930255544475102 1107 48-49 -0.07432473900446723 1107 50-51 -0.04992383689649671 1107 52-53 0.08183094558292936 1107 54-55 -0.01612936311813229 1107 56-57 0.05842117985359607 1107 58-59 -0.021587969649118577 1107 60-61 -0.0484941426895773 1107 62-63 -0.007833110455713665 1107 64-65 0.0915172415232064 1107 66-67 0.10514496360056569 1107 68-69 0.1699555726816513 1107 70-71 0.18386223174928062 1107 72-73 -0.12613138785390987 1107 74-75 0.08092788558413133 1107 76-77 0.05517311317995066 1107 78-79 -0.004843363375574228 1107 80-81 0.025609686486703254 1107 82-83 -0.017425785568470076 1107 84-85 0.12569332894868523 1107 86-87 -0.11553423075725533 1107 88-89 -0.15768582002696618 1107 90-91 -0.1495443623885464 1107 92-93 -0.1672796117736013 1107 94-95 -0.004219392339887662 1107 96-97 -0.13389675294067516 1107 98-99 -0.14443775999830422 1108 1 0.0744362543895214 1108 2 0.03978049001256778 1108 3 0.08488913670591458 1108 4 0.020054985786764234 1108 5 -0.020415974234566647 1108 6 0.1275197662484331 1108 7 0.14227927631537796 1108 8 0.09708895488935809 1108 9 0.1196396349080402 1108 10-11 0.04307509150218891 1108 12-13 -0.006475524132433463 1108 14-15 0.03419476388388887 1108 16-17 0.03346988583782462 1108 18-19 0.2881557216488062 1108 20-21 0.15829878809669395 1108 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0.2730200516804402 2215 94-95 0.3923056848921398 2215 96-97 0.44330804318563466 2215 98-99 0.4814806412708279 2216 1 -0.29902036307686686 2216 2 -0.3420527414748733 2216 3 -0.23930097077127144 2216 4 -0.2635497287129027 2216 5 -0.3648057808522083 2216 6 -0.25311670108988693 2216 7 -0.4624607229896327 2216 8 -0.3995224372699795 2216 9 -0.28894584319643 2216 10-11 -0.4671235592695808 2216 12-13 -0.45403425553687526 2216 14-15 -0.4962332102748377 2216 16-17 -0.5395306154816595 2216 18-19 -0.45713675687741784 2216 20-21 -0.264834541457482 2216 22-23 -0.26279783742618434 2216 24-25 -0.4411871857512466 2216 26-27 -0.5265791585601036 2216 28-29 -0.51781155447582 2216 30-31 -0.3043753983485047 2216 32-33 -0.25957108721316047 2216 34-35 -0.41335912206358927 2216 36-37 -0.2064457048811903 2216 38-39 -8.555105760460513 2216 40-41 -2.0537893631828013 2216 42-43 -0.6711812377574731 2216 44-45 -0.712794324017338 2216 46-47 -0.416981299154358 2216 48-49 -0.27917756048194065 2216 50-51 -0.30808718831465143 2216 52-53 -0.315154297092171 2216 54-55 -0.24128065224029172 2216 56-57 -0.17023092458286015 2216 58-59 -0.28402797803365587 2216 60-61 -0.3388406308491483 2216 62-63 -0.40726157377201133 2216 64-65 -0.38102272793314285 2216 66-67 -0.307529975080719 2216 68-69 -0.3157614547287011 2216 70-71 -0.05490984230684859 2216 72-73 -0.1270324530708855 2216 74-75 -0.13949281114889445 2216 76-77 -0.24879824329070388 2216 78-79 -0.2534332371447796 2216 80-81 -0.22370975066059628 2216 82-83 -0.35498359938280544 2216 84-85 -0.24995218491710602 2216 86-87 -0.10100149160066607 2216 88-89 -0.09206390808090958 2216 90-91 -0.05862660977042111 2216 92-93 -0.09777713044143965 2216 94-95 0.004189642268098481 2216 96-97 0.07654076730607073 2216 98-99 0.11502410534280472 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 241.0 8 1402.0 9 2716.0 10 4597.0 11 9704.0 12 19534.0 13 33591.0 14 44933.0 15 47637.0 16 50225.0 17 52836.0 18 55055.0 19 58354.0 20 62137.0 21 68206.0 22 75356.0 23 83675.0 24 94720.0 25 105431.0 26 115468.0 27 129858.0 28 151940.0 29 185344.0 30 230280.0 31 291969.0 32 380929.0 33 505409.0 34 682666.0 35 965559.0 36 1424306.0 37 1665855.0 38 529919.0 39 588.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86745128956656 19.14681176737559 12.545008299053743 24.44072864400411 2 15.945756331027386 21.03951944558832 38.025880056224416 24.988844167159886 3 17.925590884305826 26.89064342929075 28.819500778054714 26.364264908348712 4 12.69278618820987 17.672575117352125 36.75825861930066 32.87638007513734 5 14.170114784183104 37.96340485409666 33.0345115153185 14.831968846401738 6 31.05710696032013 37.56159718195006 17.622577853979703 13.758718003750104 7 27.558848059754105 32.333208739018204 22.014880718470106 18.093062482757585 8 26.745374945651808 33.60458129905559 19.87080657946767 19.77923717582494 9 26.69145415779825 15.947614183479503 19.417498058224368 37.94343360049788 10-11 25.216450342905606 26.218615722670513 26.556487778743215 22.008446155680662 12-13 26.01175791151783 24.319574074738018 26.150877065024954 23.5177909487192 14-15 24.107937566499597 25.04137496427679 23.620232346769033 27.230455122454583 16-17 23.829081715799955 27.43863643212202 24.55414747181424 24.178134380263785 18-19 24.428127857700286 26.790175691490443 25.75486950591265 23.02682694489662 20-21 26.182496776006726 25.881393945841836 24.463070594536955 23.47303868361449 22-23 25.173775979378238 25.860657708642794 24.479004147254532 24.486562164724436 24-25 24.415467905183412 26.54319996856232 24.532799581519686 24.508532544734585 26-27 24.676387564958027 26.33507579717721 24.43294486639402 24.55559177147074 28-29 24.260052086626057 26.289134155164223 25.03300615401494 24.417807604194778 30-31 24.580379296649713 26.320084021923535 24.778238922031882 24.321297759394874 32-33 24.25045994126232 26.501121840955644 24.267753093149576 24.98066512463246 34-35 24.75631712118059 26.438113342887547 24.93690381484701 23.86866572108485 36-37 24.562903180278596 26.417391786700723 24.51012002985572 24.50958500316496 38-39 24.65150572368959 26.07923448174691 24.734380338114644 24.53487945644886 40-41 24.418358223660057 26.4429253494377 24.935945082325222 24.20277134457702 42-43 24.956719627251616 26.230153617572526 24.751471864759715 24.061654890416147 44-45 24.391530844867653 26.669241351488143 24.460690651003727 24.478537152640474 46-47 23.898531605065156 26.55136159787975 25.13883125179727 24.41127554525782 48-49 24.573059515316306 26.640322447632425 24.724859483251606 24.061758553799663 50-51 23.89348758941237 26.797006264101054 24.645180945802448 24.664325200684125 52-53 24.064831119045376 26.40778612018216 24.615540913555904 24.91184184721656 54-55 24.246803219257988 26.318940722186795 24.74636997113934 24.68788608741588 56-57 24.411259268297123 25.884833029207403 24.48378333967168 25.220124362823793 58-59 24.157707490654417 25.88200436027821 25.08880664519634 24.87148150387104 60-61 24.413023555806408 26.08496935650568 24.80153280813811 24.700474279549795 62-63 23.898101247373603 26.262646451122034 24.56167517563311 25.277577125871247 64-65 23.972073648906317 25.9208988306889 24.786721367639945 25.320306152764836 66-67 24.5565176513825 26.031160687550543 24.587826303215714 24.824495357851248 68-69 24.586811585566434 25.701604994280373 24.63891272450397 25.07267069564922 70-71 24.028904211413366 25.765661065269335 24.833282423838853 25.372152299478444 72-73 24.715710007449296 25.842286110405414 24.86742645426856 24.574577427876726 74-75 24.962935019991843 25.79634051124212 24.726591192765447 24.51413327600059 76-77 24.554799343454565 25.89873112570238 24.983958422888815 24.56251110795425 78-79 24.537075928391058 25.855135461968402 24.994861899797023 24.61292670984352 80-81 24.23230831768741 25.707707265715467 25.4095255548743 24.65045886172282 82-83 24.621558673188098 25.648903934439314 25.010880432213757 24.71865696015883 84-85 24.60132201327056 25.804343634893844 24.638786333148943 24.955548018686653 86-87 24.4449965530792 25.48260888549151 24.970576641253952 25.10181792017534 88-89 24.5572212635141 25.00100394277385 25.147379721659714 25.294395072052346 90-91 24.772973975882245 25.22289646125059 24.97430477133322 25.029824791533944 92-93 24.732145657464013 25.369847529870754 24.80372845520756 25.09427835745768 94-95 24.05086068474727 25.543232873477407 25.293142011292126 25.112764430483203 96-97 24.535027328325754 25.499522828994927 24.766454343301632 25.19899549937769 98-99 24.38366279045811 25.6253008508602 24.701704469452988 25.289331889228706 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1363.0 1 1224.5 2 1084.0 3 1099.5 4 1027.0 5 911.0 6 812.5 7 728.0 8 728.0 9 740.0 10 708.5 11 672.0 12 668.0 13 702.0 14 765.5 15 942.5 16 1181.5 17 1462.5 18 1998.5 19 2784.5 20 3820.0 21 5296.0 22 7236.5 23 9883.5 24 13136.5 25 17325.5 26 22677.5 27 28841.0 28 37498.0 29 49244.5 30 59644.0 31 70869.0 32 83755.0 33 95407.5 34 105777.5 35 118555.5 36 130647.5 37 144740.5 38 158354.5 39 157935.5 40 161435.5 41 172186.0 42 182301.0 43 205532.5 44 233577.0 45 263526.5 46 307564.0 47 341799.5 48 358136.0 49 377611.0 50 404802.0 51 398302.5 52 372262.5 53 358698.5 54 334663.0 55 319245.0 56 298143.0 57 273610.0 58 259590.0 59 230927.0 60 191786.0 61 147694.5 62 119671.5 63 98166.5 64 73397.0 65 58011.0 66 44157.5 67 33952.0 68 27001.5 69 22428.5 70 19625.5 71 17915.5 72 18100.5 73 16306.5 74 12157.5 75 9530.0 76 7687.0 77 5936.0 78 4171.0 79 2463.0 80 1329.0 81 901.0 82 692.0 83 541.0 84 381.0 85 299.0 86 178.5 87 120.5 88 93.0 89 69.5 90 55.0 91 32.5 92 25.5 93 22.5 94 15.5 95 15.5 96 11.5 97 5.5 98 3.5 99 8.5 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0020786070135152507 3 0.006813895180399106 4 0.0 5 0.0049812771625661325 6 0.0 7 7.133680874786066E-4 8 0.0 9 0.0 10-11 6.149724892056954E-6 12-13 5.534752402851258E-5 14-15 0.0027858253761018 16-17 1.2299449784113908E-4 18-19 0.0 20-21 0.0 22-23 7.379669870468345E-5 24-25 9.163090089164861E-4 26-27 0.0023983927079022117 28-29 0.0017588213191282887 30-31 0.002287697659845187 32-33 7.256675372627206E-4 34-35 1.2299449784113909E-5 36-37 1.1684477294908212E-4 38-39 0.06047639458848809 40-41 0.0038374283326435394 42-43 3.3823486906313247E-4 44-45 0.0 46-47 8.732609346720875E-4 48-49 1.0454532316496821E-4 50-51 8.425123102118028E-4 52-53 3.5053431884724635E-4 54-55 0.0 56-57 5.042774411486702E-4 58-59 6.149724892056954E-6 60-61 9.839559827291127E-5 62-63 2.4598899568227817E-5 64-65 7.318172621547774E-4 66-67 0.0012852925024399034 68-69 0.0012852925024399034 70-71 4.304807424439868E-5 72-73 0.0018018693933726875 74-75 0.0015866290221506942 76-77 1.2299449784113908E-4 78-79 9.839559827291127E-5 80-81 0.0 82-83 2.890370699266768E-4 84-85 5.22726615824841E-4 86-87 0.0 88-89 1.8449174676170862E-5 90-91 5.473255153930689E-4 92-93 0.0 94-95 0.0 96-97 0.0029026701490508823 98-99 0.004655341743287114 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 8130445.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.024647321430564 #Duplication Level Percentage of deduplicated Percentage of total 1 73.78359703406836 27.318116582929314 2 13.868563237970319 10.269573254816164 3 4.976051965357936 5.527097072114671 4 2.3297491123610334 3.4503255693033266 5 1.2290979128458275 2.2753458373311584 6 0.727783203749522 1.616754986717214 7 0.5088645816942059 1.3188372170116685 8 0.3551895603814106 1.0520614564300554 9 0.2617171390934132 0.8720986295616671 >10 1.5783034215602885 11.182081570911965 >50 0.17631303536153375 4.605316867115921 >100 0.16473660068509416 12.757701872737357 >500 0.02425559526067858 6.216332158537934 >1k 0.015272985550686735 10.251099822117295 >5k 4.373321849333775E-4 0.8983111277005537 >10k+ 6.7281874605135E-5 0.3889459746638361 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 16345 0.20103450672134182 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 14882 0.18304041168718319 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.2299449784113907E-5 2.4598899568227814E-5 2 0.0 0.0 0.0 1.2299449784113907E-5 2.4598899568227814E-5 3 0.0 0.0 0.0 1.2299449784113907E-5 2.4598899568227814E-5 4 0.0 1.2299449784113907E-5 0.0 4.919779913645563E-5 2.4598899568227814E-5 5 0.0 1.2299449784113907E-5 0.0 8.609614848879736E-5 3.6898349352341724E-5 6 0.0 1.2299449784113907E-5 0.0 8.609614848879736E-5 4.919779913645563E-5 7 0.0 1.2299449784113907E-5 0.0 8.609614848879736E-5 6.149724892056954E-5 8 0.0 1.2299449784113907E-5 0.0 8.609614848879736E-5 6.149724892056954E-5 9 1.2299449784113907E-5 1.2299449784113907E-5 0.0 1.1069504805702517E-4 6.149724892056954E-5 10-11 1.844917467617086E-5 1.2299449784113907E-5 0.0 1.2299449784113908E-4 6.149724892056954E-5 12-13 2.4598899568227814E-5 1.2299449784113907E-5 0.0 1.598928471934808E-4 6.149724892056954E-5 14-15 3.074862446028477E-5 1.2299449784113907E-5 0.0 1.598928471934808E-4 7.379669870468345E-5 16-17 3.6898349352341724E-5 1.2299449784113907E-5 0.0 1.9064147165376557E-4 8.609614848879736E-5 18-19 5.534752402851258E-5 1.2299449784113907E-5 0.0 2.4598899568227815E-4 8.609614848879736E-5 20-21 1.0454532316496821E-4 1.2299449784113907E-5 0.0 4.7352881668838545E-4 8.609614848879736E-5 22-23 1.2914422273319602E-4 3.6898349352341724E-5 0.0 0.00171577324488389 9.839559827291126E-5 24-25 1.598928471934808E-4 4.304807424439867E-5 0.0 0.004015770354513191 1.1684477294908212E-4 26-27 2.2139009611405033E-4 4.919779913645563E-5 0.0 0.006537157560256543 1.35293947625253E-4 28-29 2.2139009611405033E-4 4.919779913645563E-5 0.0 0.017385272269845008 1.598928471934808E-4 30-31 2.275398210061073E-4 6.149724892056954E-5 0.0 0.047937105533583955 1.598928471934808E-4 32-33 2.3368954589816424E-4 6.149724892056954E-5 0.0 0.10153195796786031 1.7219229697759472E-4 34-35 2.3368954589816424E-4 7.379669870468345E-5 0.0 0.1708516569511263 1.8449174676170863E-4 36-37 2.3368954589816424E-4 9.224587338085431E-5 0.0 0.27697745941335317 1.8449174676170863E-4 38-39 2.3368954589816424E-4 9.839559827291126E-5 0.0 0.44223901643759966 1.8449174676170863E-4 40-41 2.3368954589816424E-4 9.839559827291126E-5 0.0 0.6402909065862938 1.8449174676170863E-4 42-43 2.5213872057433507E-4 9.839559827291126E-5 0.0 0.8201826591287439 1.8449174676170863E-4 44-45 2.5828844546639204E-4 1.1069504805702517E-4 0.0 1.022871441846049 1.8449174676170863E-4 46-47 2.5828844546639204E-4 1.2299449784113908E-4 0.0 1.2500914771577694 1.8449174676170863E-4 48-49 2.5828844546639204E-4 1.2299449784113908E-4 0.0 1.4936697806823611 1.967911965458225E-4 50-51 2.5828844546639204E-4 1.2299449784113908E-4 0.0 1.7662993845970298 2.0909064632993642E-4 52-53 2.5828844546639204E-4 1.2299449784113908E-4 0.0 2.0353068005502775 2.0909064632993642E-4 54-55 2.5828844546639204E-4 1.2299449784113908E-4 0.0 2.3017005834244006 2.2139009611405033E-4 56-57 2.5828844546639204E-4 1.2299449784113908E-4 0.0 2.6009031485976477 2.2139009611405033E-4 58-59 2.70587895250506E-4 1.2299449784113908E-4 0.0 2.9324778656026824 2.2139009611405033E-4 60-61 2.70587895250506E-4 1.35293947625253E-4 0.0 3.2699440190543076 2.2139009611405033E-4 62-63 2.70587895250506E-4 1.35293947625253E-4 0.0 3.613941180341297 2.3368954589816424E-4 64-65 2.70587895250506E-4 1.8449174676170863E-4 0.0 3.9671813781410488 2.4598899568227815E-4 66-67 2.70587895250506E-4 1.8449174676170863E-4 0.0 4.3488333049421035 2.5828844546639204E-4 68-69 2.70587895250506E-4 1.967911965458225E-4 0.0 4.7628327846753775 2.5828844546639204E-4 70-71 2.70587895250506E-4 2.0909064632993642E-4 0.0 5.232419627707954 2.5828844546639204E-4 72-73 2.70587895250506E-4 2.0909064632993642E-4 0.0 5.695088030236967 3.013365197107907E-4 74-75 2.70587895250506E-4 2.0909064632993642E-4 0.0 6.1428126012782815 3.197856943869616E-4 76-77 2.70587895250506E-4 2.1524037122199336E-4 0.0 6.619133393067661 3.197856943869616E-4 78-79 2.70587895250506E-4 2.275398210061073E-4 0.0 7.116972072254347 3.197856943869616E-4 80-81 2.70587895250506E-4 2.3368954589816424E-4 0.0 7.663338230564255 3.197856943869616E-4 82-83 2.70587895250506E-4 2.3983927079022118E-4 0.0 8.24063750508121 3.566840437393033E-4 84-85 2.70587895250506E-4 2.5828844546639204E-4 0.0 8.804641074381538 3.628337686313603E-4 86-87 2.70587895250506E-4 2.5828844546639204E-4 0.0 9.384455586379342 3.751332184154742E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 3125 0.0 22.02301 1 GTACATA 2270 0.0 20.48515 1 GTACAAG 3795 0.0 20.463018 1 GTATCAA 40035 0.0 20.407833 1 GTATAAG 3760 0.0 19.911457 1 CTAACGC 1430 0.0 19.511032 3 TAGTACT 3810 0.0 19.037437 4 TACATGA 2935 0.0 19.012451 2 CATGGGG 21310 0.0 18.763935 4 GTACATG 59435 0.0 18.636505 1 TCTAACG 1455 0.0 18.536596 2 TACATGG 58490 0.0 17.94382 2 GTGATCG 2305 0.0 17.750475 8 GTTATAT 2635 0.0 17.471073 1 TATATAC 4105 0.0 17.445065 3 GTACAAA 3930 0.0 17.275288 1 CTCGTCG 2655 0.0 17.163261 9 TGATCGC 2385 0.0 17.156654 9 ACGTTAA 845 0.0 17.05702 8 ATTTAGG 3470 0.0 17.019203 1 >>END_MODULE