FastQCFastQC Report
Fri 27 May 2016
ERR765524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8130445
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246320.3029600470822938No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT165710.20381418237255156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159150.19574574331417283No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA153180.18840297179305684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133870.16465273425993288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG5950.031.2628022
GTATCAA494100.031.1510981
ATCAACG623250.024.353093
CAACGCA623250.024.3232465
AACGCAG649550.023.6598366
TATCAAC659550.023.4508952
TCAACGC650000.023.3866394
GGTATCA331850.022.9384561
AGTACTT362150.021.95771812-13
ACGCAGA692800.021.6928377
CGCAGAG698650.021.4651348
GTACTAG29700.020.9928341
TATTCGC5200.020.5680649
GCAGAGT738350.020.1852749
GAGTACT348150.019.44798312-13
TACTTTT425200.018.91479114-15
AGAGTAC723700.018.27792510-11
TAGTACT37200.018.1255554
CTAGGCA48700.017.9512674
TATATAC38500.017.8758723
Produced by FastQC (version 0.11.5)