##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765524_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8130445 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28536961999005 33.0 33.0 33.0 33.0 33.0 2 32.12637807647675 33.0 33.0 33.0 33.0 33.0 3 32.20515248550356 33.0 33.0 33.0 33.0 33.0 4 36.12407242162022 37.0 37.0 37.0 33.0 37.0 5 36.065631585971005 37.0 37.0 37.0 33.0 37.0 6 36.0656774629187 37.0 37.0 37.0 33.0 37.0 7 35.97771450394167 37.0 37.0 37.0 33.0 37.0 8 35.93721782756048 37.0 37.0 37.0 33.0 37.0 9 35.683666515178444 37.0 37.0 37.0 33.0 37.0 10-11 35.861647548196935 37.0 37.0 37.0 33.0 37.0 12-13 35.78595944010445 37.0 37.0 37.0 33.0 37.0 14-15 37.15438712149212 37.0 37.0 40.0 33.0 40.0 16-17 37.30693215439007 37.0 37.0 40.0 33.0 40.0 18-19 37.37387307828784 37.0 37.0 40.0 33.0 40.0 20-21 37.3998719012305 37.0 37.0 40.0 33.0 40.0 22-23 37.315147202889875 37.0 37.0 40.0 33.0 40.0 24-25 37.2610026265475 37.0 37.0 40.0 33.0 40.0 26-27 37.12534965798305 37.0 37.0 40.0 33.0 40.0 28-29 37.05695555409329 37.0 37.0 40.0 33.0 40.0 30-31 36.946277479276965 37.0 37.0 40.0 33.0 40.0 32-33 36.76891603104135 37.0 37.0 40.0 33.0 40.0 34-35 36.67637140402524 37.0 37.0 40.0 33.0 40.0 36-37 36.51413815602959 37.0 37.0 40.0 33.0 40.0 38-39 36.36146637730161 37.0 37.0 40.0 33.0 40.0 40-41 36.22717680274573 37.0 37.0 40.0 33.0 40.0 42-43 36.04259004273444 37.0 37.0 40.0 33.0 40.0 44-45 35.82883722846658 37.0 37.0 40.0 30.0 40.0 46-47 35.56361097086322 37.0 37.0 40.0 27.0 40.0 48-49 35.306625467609706 37.0 33.0 40.0 27.0 40.0 50-51 34.995795125605056 37.0 33.0 40.0 27.0 40.0 52-53 34.8382192487619 37.0 33.0 40.0 27.0 40.0 54-55 34.70948957898368 37.0 33.0 40.0 27.0 40.0 56-57 34.53813499753089 37.0 33.0 40.0 27.0 40.0 58-59 34.353703702564864 37.0 33.0 38.5 27.0 40.0 60-61 34.18029954080004 37.0 33.0 37.0 27.0 40.0 62-63 33.86898865929233 37.0 33.0 37.0 27.0 40.0 64-65 33.72887800114262 37.0 33.0 37.0 27.0 40.0 66-67 33.54847600592588 37.0 33.0 37.0 27.0 40.0 68-69 33.31574944544856 37.0 33.0 37.0 27.0 40.0 70-71 33.11846307305443 37.0 33.0 37.0 24.5 37.0 72-73 32.91963607158034 37.0 33.0 37.0 22.0 37.0 74-75 32.7167468693288 37.0 33.0 37.0 22.0 37.0 76-77 32.55697664764967 35.0 33.0 37.0 22.0 37.0 78-79 32.37264571619389 33.0 33.0 37.0 22.0 37.0 80-81 32.225537532078505 33.0 33.0 37.0 22.0 37.0 82-83 32.08766814116571 33.0 33.0 37.0 22.0 37.0 84-85 31.9354536559807 33.0 33.0 37.0 22.0 37.0 86-87 31.842592564121645 33.0 33.0 37.0 22.0 37.0 88-89 31.736818833409487 33.0 33.0 37.0 22.0 37.0 90-91 31.675769468952808 33.0 33.0 37.0 22.0 37.0 92-93 31.577284576674465 33.0 33.0 37.0 22.0 37.0 94-95 31.503390404830242 33.0 33.0 37.0 22.0 37.0 96-97 31.459231813264836 33.0 33.0 37.0 22.0 37.0 98-99 30.641470915798582 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1516371564130452 1101 2 0.3263132149889074 1101 3 0.23782259050336307 1101 4 0.1758488546829966 1101 5 0.012319018895063039 1101 6 0.14166227969557355 1101 7 0.20390240774415247 1101 8 0.13393871090151777 1101 9 0.39308475984713453 1101 10-11 0.30903550986881356 1101 12-13 0.2958336568250246 1101 14-15 0.5128049493609907 1101 16-17 0.418190170925115 1101 18-19 0.2765760266309485 1101 20-21 0.35726510784427035 1101 22-23 0.22970040839327055 1101 24-25 0.29896816891122313 1101 26-27 0.21384895003073012 1101 28-29 0.17668601132271533 1101 30-31 0.19602839628262103 1101 32-33 0.31707205617644263 1101 34-35 0.4069225118081832 1101 36-37 0.36066444379676454 1101 38-39 0.12671624120219604 1101 40-41 0.10075981196135331 1101 42-43 0.2780565108961284 1101 44-45 0.3011284036176818 1101 46-47 0.41521757982472707 1101 48-49 0.40374474015153083 1101 50-51 0.3560326457841967 1101 52-53 0.4058889304541182 1101 54-55 0.4119994637555422 1101 56-57 0.4937422737643331 1101 58-59 0.4854134199770357 1101 60-61 0.33721332895020595 1101 62-63 0.36627288828901783 1101 64-65 0.31965886313685843 1101 66-67 0.3775084911471396 1101 68-69 0.27450438435846536 1101 70-71 0.20346936908322277 1101 72-73 0.308135531141545 1101 74-75 0.21191633854564884 1101 76-77 0.22886334122955532 1101 78-79 0.540886542667117 1101 80-81 0.474624348121651 1101 82-83 0.44859512380580213 1101 84-85 0.41502380852448795 1101 86-87 0.19222955850435852 1101 88-89 0.3424169060349058 1101 90-91 0.2626976348022758 1101 92-93 0.4539822784150047 1101 94-95 0.4647149884109929 1101 96-97 0.2657505546586343 1101 98-99 0.34050575519112414 1102 1 0.08903994844533969 1102 2 0.3083715323996614 1102 3 0.19109826946989728 1102 4 0.17191157316806738 1102 5 0.0643086637805439 1102 6 0.2174455685613097 1102 7 0.17145134550979435 1102 8 0.15303084640385833 1102 9 0.37116444711059415 1102 10-11 0.3352853868001304 1102 12-13 0.2948774366185347 1102 14-15 0.5151956271194322 1102 16-17 0.3299194423935603 1102 18-19 0.25676356782179965 1102 20-21 0.35920269132026306 1102 22-23 0.2088328774404289 1102 24-25 0.3857534004908487 1102 26-27 0.26576369505482944 1102 28-29 0.24104414984515188 1102 30-31 0.22421357650932094 1102 32-33 0.32741678772548966 1102 34-35 0.39031025595139823 1102 36-37 0.31031619303325186 1102 38-39 0.16370452693716686 1102 40-41 0.2282222034359478 1102 42-43 0.23815976317473542 1102 44-45 0.27852688692296823 1102 46-47 0.4695115406279342 1102 48-49 0.496126491817229 1102 50-51 0.4047874880123885 1102 52-53 0.4097049246833109 1102 54-55 0.3518018710399389 1102 56-57 0.4995501482703517 1102 58-59 0.3949976115337108 1102 60-61 0.27314634098980406 1102 62-63 0.40486259372958244 1102 64-65 0.2892056462946897 1102 66-67 0.39609009013845053 1102 68-69 0.3320616704353796 1102 70-71 0.2906713749255303 1102 72-73 0.4252865997848403 1102 74-75 0.33534419473041766 1102 76-77 0.2832843203081694 1102 78-79 0.45468088413833385 1102 80-81 0.35039281988515825 1102 82-83 0.371895548432434 1102 84-85 0.4399149421154718 1102 86-87 0.2635339211657808 1102 88-89 0.31570309378383143 1102 90-91 0.28332253010737674 1102 92-93 0.358962347600567 1102 94-95 0.4336873594625068 1102 96-97 0.3274482822713516 1102 98-99 0.3070836413969218 1103 1 0.11724614943125289 1103 2 0.28305208059698117 1103 3 0.23008724095087274 1103 4 0.18278404009924998 1103 5 0.08695258639117753 1103 6 0.1606130392347609 1103 7 0.13149475721741055 1103 8 0.07723123109773411 1103 9 0.4041226487791647 1103 10-11 0.259119940614319 1103 12-13 0.26580610509367375 1103 14-15 0.4271784033390915 1103 16-17 0.35251978705552034 1103 18-19 0.25082308098151174 1103 20-21 0.31753841185509657 1103 22-23 0.19187485823482575 1103 24-25 0.26628136064500296 1103 26-27 0.11828885149989787 1103 28-29 0.08178278501476655 1103 30-31 0.0993125494311613 1103 32-33 0.14546204692094022 1103 34-35 0.25176512798918793 1103 36-37 0.24522230420542002 1103 38-39 0.10219345134443358 1103 40-41 0.08257589087547501 1103 42-43 0.05651564177868096 1103 44-45 0.10819722587475411 1103 46-47 0.27171312192227504 1103 48-49 0.13760356239905747 1103 50-51 0.0803449980176083 1103 52-53 0.0647865468649087 1103 54-55 0.07107745819514832 1103 56-57 0.17226808669621363 1103 58-59 0.1843006830953584 1103 60-61 0.09075724514308803 1103 62-63 0.1776915014142162 1103 64-65 -0.03579637557866988 1103 66-67 0.02625801606438216 1103 68-69 0.07882679873208787 1103 70-71 0.030036051200731606 1103 72-73 0.08358657849190365 1103 74-75 0.07396219727638709 1103 76-77 0.029661872322094496 1103 78-79 0.27047349989145886 1103 80-81 0.09210583881575474 1103 82-83 0.15865485024814063 1103 84-85 0.24642540117506684 1103 86-87 0.16137409453107665 1103 88-89 0.12408795430440023 1103 90-91 -0.04178614747147691 1103 92-93 -0.03711789937197807 1103 94-95 0.1843400449879482 1103 96-97 0.14201579191090374 1103 98-99 0.08647902400457141 1104 1 0.10312132206465208 1104 2 0.27147123241100957 1104 3 0.18875123107833502 1104 4 0.1444377225798661 1104 5 -0.04676196793306531 1104 6 0.09965102927706937 1104 7 0.11095700909528006 1104 8 0.009089346885559735 1104 9 0.31029941677464024 1104 10-11 0.21312598573828012 1104 12-13 0.21234495478526583 1104 14-15 0.35091768205742113 1104 16-17 0.19369784858065486 1104 18-19 0.14387608841644095 1104 20-21 0.21357684071086425 1104 22-23 0.1211001495173818 1104 24-25 0.17242129267692263 1104 26-27 0.04009572205932699 1104 28-29 0.056188378836921515 1104 30-31 0.15244118282750918 1104 32-33 0.1925683015589641 1104 34-35 0.203020702631882 1104 36-37 0.13758526140955496 1104 38-39 -0.02516692244225993 1104 40-41 -0.017216707353810534 1104 42-43 0.12152362180052023 1104 44-45 0.19504239345388186 1104 46-47 0.2617291786413105 1104 48-49 0.36035151468565374 1104 50-51 0.25283352924000724 1104 52-53 0.2845322820410132 1104 54-55 0.22397538178704224 1104 56-57 0.2606375765065252 1104 58-59 0.2819947305877264 1104 60-61 0.2706515034804582 1104 62-63 0.25126845159640254 1104 64-65 0.14887856243817765 1104 66-67 0.18041005754685813 1104 68-69 0.14013858149273517 1104 70-71 0.2257130168178776 1104 72-73 0.2209723997514672 1104 74-75 0.1346852071858109 1104 76-77 0.12465475377048563 1104 78-79 0.3785721907414441 1104 80-81 0.23722315006984473 1104 82-83 0.23054381541050617 1104 84-85 0.22083045533677392 1104 86-87 0.2236925360394011 1104 88-89 0.15417735782996544 1104 90-91 0.15026431322969103 1104 92-93 0.11115947470521448 1104 94-95 0.19267439859955715 1104 96-97 0.13860931284697742 1104 98-99 0.07150894271316588 1105 1 0.12667575672057296 1105 2 0.2849619895583402 1105 3 0.20504532421610122 1105 4 0.1777081094545636 1105 5 -0.02108674467547189 1105 6 0.10407050955146246 1105 7 0.10220709495842328 1105 8 -0.007147997076621948 1105 9 0.3166307089217639 1105 10-11 0.19940089696111585 1105 12-13 0.20601757874347726 1105 14-15 0.3253336710110304 1105 16-17 0.28971813090482357 1105 18-19 0.19267203684591294 1105 20-21 0.2513651045050054 1105 22-23 0.10646226795016389 1105 24-25 0.2721771305430778 1105 26-27 0.08354607874029796 1105 28-29 0.10438377926291764 1105 30-31 0.17563244696947322 1105 32-33 0.1885048073646658 1105 34-35 0.23715430446848274 1105 36-37 0.2012147625375178 1105 38-39 0.14697782487223776 1105 40-41 0.04470886591591494 1105 42-43 0.10775441876741354 1105 44-45 0.127107404414339 1105 46-47 0.3043772539125129 1105 48-49 0.2856490670432663 1105 50-51 0.15382229120201174 1105 52-53 0.12311027280664888 1105 54-55 0.19527622835806113 1105 56-57 0.21608739947173916 1105 58-59 0.1840072318428838 1105 60-61 0.14391245008759768 1105 62-63 0.16705574704926107 1105 64-65 0.067788875015097 1105 66-67 0.11717227491545401 1105 68-69 0.09476191320732141 1105 70-71 0.17698264106950035 1105 72-73 0.21231354733220797 1105 74-75 0.07712156020124894 1105 76-77 0.0907408267951908 1105 78-79 0.19207233934407242 1105 80-81 0.1637507488238441 1105 82-83 0.15934564735476897 1105 84-85 0.10108424762186985 1105 86-87 0.0899777016347798 1105 88-89 0.05497401143322378 1105 90-91 0.07553046804099495 1105 92-93 0.04314620608971609 1105 94-95 0.25383665848848125 1105 96-97 0.13889567273343673 1105 98-99 0.1051869905917826 1106 1 0.07541083630602685 1106 2 0.21532908899664704 1106 3 0.1481809709539874 1106 4 0.1132201088954119 1106 5 -7.888913656799446E-4 1106 6 0.14352660039563858 1106 7 0.10103265874242595 1106 8 0.05320819612771999 1106 9 0.3503401952888865 1106 10-11 0.17914292086788208 1106 12-13 0.18308935914009083 1106 14-15 0.36455140942386777 1106 16-17 0.26049292862584394 1106 18-19 0.18211988417282754 1106 20-21 0.31325464184041607 1106 22-23 0.14059718152685008 1106 24-25 0.21956542861504147 1106 26-27 0.06378351758078082 1106 28-29 0.11284890325288899 1106 30-31 0.09422504132498233 1106 32-33 0.08311478922387039 1106 34-35 0.20293737711713078 1106 36-37 0.09689739030592648 1106 38-39 0.04012772750434124 1106 40-41 0.054418079969167366 1106 42-43 0.05184252222287 1106 44-45 0.06638611198589928 1106 46-47 0.24680184015429774 1106 48-49 0.309837315278763 1106 50-51 0.1172141863013465 1106 52-53 0.14237863460358824 1106 54-55 0.12073068940335219 1106 56-57 0.22317381618771748 1106 58-59 0.11774618624774291 1106 60-61 0.14914370750570072 1106 62-63 0.16881491512507552 1106 64-65 -0.02747833666353472 1106 66-67 0.10666732530101086 1106 68-69 0.021747715122423017 1106 70-71 0.07198857496917554 1106 72-73 0.11017765204081797 1106 74-75 0.19532217467862267 1106 76-77 0.11181276553372754 1106 78-79 0.19739966578078594 1106 80-81 0.11667329919790603 1106 82-83 0.030559080843474362 1106 84-85 0.0071771822562531895 1106 86-87 0.08006112735206017 1106 88-89 -0.03018730480899734 1106 90-91 0.00913855891883486 1106 92-93 -0.04505537027720763 1106 94-95 0.18577896956955087 1106 96-97 0.13189341568038415 1106 98-99 0.04965780446606516 1107 1 0.10723051554251839 1107 2 0.24677164968980492 1107 3 0.2368291234426394 1107 4 0.1754015701845546 1107 5 0.046319390087475654 1107 6 0.11090189250630544 1107 7 0.09457947918502185 1107 8 0.04453301808286625 1107 9 0.38416786431390193 1107 10-11 0.19778883424871907 1107 12-13 0.19028757107500383 1107 14-15 0.30518714135504155 1107 16-17 0.2552530887740261 1107 18-19 0.14364880700039606 1107 20-21 0.2911123779074529 1107 22-23 0.13575756192412314 1107 24-25 0.2117976228404288 1107 26-27 0.023498197598676995 1107 28-29 0.10846681165480732 1107 30-31 0.038467097520559435 1107 32-33 -0.007271431347348312 1107 34-35 0.19183102932817775 1107 36-37 0.14375615377250028 1107 38-39 0.04202027187483992 1107 40-41 0.04978534070130536 1107 42-43 0.05392657313906568 1107 44-45 0.06958997241252263 1107 46-47 0.10625657502039587 1107 48-49 0.23350028615004703 1107 50-51 0.09564815230797308 1107 52-53 0.11140347875416978 1107 54-55 0.12186123721519238 1107 56-57 0.16596972642910401 1107 58-59 0.08357074204258197 1107 60-61 0.16422405357221947 1107 62-63 0.1930344360973706 1107 64-65 -0.014158721765340943 1107 66-67 0.008025285281981098 1107 68-69 -0.005067701074665365 1107 70-71 0.06868097617662272 1107 72-73 0.14997532123680202 1107 74-75 0.05723001660045668 1107 76-77 0.13199677729409132 1107 78-79 0.0836058919217848 1107 80-81 0.0451320560706705 1107 82-83 0.02434608203083144 1107 84-85 0.02595864499819811 1107 86-87 0.0598912965868692 1107 88-89 0.06272171291108464 1107 90-91 0.02626914429421845 1107 92-93 0.029667698143292398 1107 94-95 0.17543817463244338 1107 96-97 0.060521779653679886 1107 98-99 -0.0069940469209228695 1108 1 0.10957533732450031 1108 2 0.21034612292705646 1108 3 0.14835683931270438 1108 4 0.05383643360310231 1108 5 -0.05971569989706893 1108 6 0.05961547005005485 1108 7 0.0852207342912763 1108 8 0.03077373682148732 1108 9 0.30039172053885466 1108 10-11 0.2250821715922413 1108 12-13 0.15349492921913566 1108 14-15 0.26963910829964277 1108 16-17 0.23386180755531427 1108 18-19 0.008100113747296689 1108 20-21 0.17483856507271156 1108 22-23 0.08569581858873221 1108 24-25 0.10307892817665021 1108 26-27 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-0.11945637291821143 2215 94-95 -0.10794847565173527 2215 96-97 -0.07797593651826062 2215 98-99 0.03161583107700494 2216 1 -0.2243002336482931 2216 2 0.025850058697187706 2216 3 -0.17308204949382855 2216 4 -0.30406312629802557 2216 5 -0.21717821297016116 2216 6 -0.2393756410250205 2216 7 -0.23504297176681632 2216 8 -0.20306663673565595 2216 9 0.06815122591327594 2216 10-11 -0.2012782501928072 2216 12-13 -0.11704236307372895 2216 14-15 -0.39956563738308404 2216 16-17 -0.5807040412107582 2216 18-19 -0.562456250126047 2216 20-21 -0.8773187613819857 2216 22-23 -0.7625711118222114 2216 24-25 -0.5995830214075895 2216 26-27 -0.6674024048355633 2216 28-29 -0.7528719099214811 2216 30-31 -0.5103020365040507 2216 32-33 -0.5549360118620186 2216 34-35 -0.5561476989992968 2216 36-37 -0.5114700244859733 2216 38-39 -0.5136164218963728 2216 40-41 -0.44466943317488017 2216 42-43 -0.4886303831660683 2216 44-45 -0.38096713633869683 2216 46-47 -0.6496615995466968 2216 48-49 -0.8590998178399545 2216 50-51 -0.4456824297366069 2216 52-53 -0.36868393273863376 2216 54-55 -0.3672252568788821 2216 56-57 -0.42414564258569243 2216 58-59 -0.33712939379776685 2216 60-61 -0.38038411917096937 2216 62-63 -0.47260367324457775 2216 64-65 -0.4704060165280026 2216 66-67 -0.42897024750345025 2216 68-69 -0.36117221375082664 2216 70-71 -0.39616318854136523 2216 72-73 -0.2639048942566262 2216 74-75 -0.3547384978397332 2216 76-77 -0.25176853196727933 2216 78-79 -0.26891545733490574 2216 80-81 -0.2522460074830164 2216 82-83 -0.19665445425269468 2216 84-85 -0.22884544817902253 2216 86-87 -0.32051747668497654 2216 88-89 -0.21282933883075117 2216 90-91 -0.18281745868991095 2216 92-93 -0.21275627618043202 2216 94-95 -0.28161422598496344 2216 96-97 -0.2834823255014456 2216 98-99 -0.1695015671404434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 40.0 10 124.0 11 252.0 12 352.0 13 714.0 14 1763.0 15 4469.0 16 10094.0 17 18197.0 18 28791.0 19 40513.0 20 52172.0 21 65951.0 22 79233.0 23 76886.0 24 65918.0 25 64616.0 26 76547.0 27 98179.0 28 127919.0 29 168852.0 30 220317.0 31 288222.0 32 377542.0 33 493102.0 34 653298.0 35 916165.0 36 1468809.0 37 2029332.0 38 701190.0 39 879.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53312388051344 19.232223737945233 13.25924955097475 24.975402830566576 2 15.886559612822108 21.355240711244786 37.43125140916699 25.32694826676612 3 17.837424642808607 26.233680936283317 30.073298570988428 25.855595849919656 4 13.021009305148734 17.469953981608633 36.72791587668326 32.781120836559374 5 13.87971326799701 37.30365893257764 33.44844678631873 15.368181013106625 6 31.05382300722777 37.64566392122448 17.206622761730753 14.09389030981699 7 26.78855831384383 32.02285975736876 22.66613943025259 18.522442498534826 8 27.267154503843273 32.1645863172311 20.22579329913676 20.342465879788868 9 26.930066645190838 13.74814900094944 20.164367608688192 39.15741674517154 10-11 25.498696565809126 25.030007582610793 27.231768986814377 22.239526864765704 12-13 26.698753881245125 23.09006456596164 27.16898644539136 23.04219510740187 14-15 24.089086516171157 23.634862057740914 25.605470151741685 26.670581274346244 16-17 23.13424345147895 26.16905368704859 26.39116712258674 24.305535738885716 18-19 23.636965750337158 24.74759376639286 29.030028491675424 22.585411991594555 20-21 24.937017592518 24.034170331390225 28.149430935207114 22.879381140884664 22-23 24.029767128367514 23.936998528370836 28.2020848797329 23.83114946352875 24-25 23.57003214461201 24.609600089539995 28.194373124718265 23.62599464112973 26-27 23.871737647816325 24.40189313131077 28.037881075390093 23.68848814548281 28-29 23.44243150282672 24.27201709131542 28.653407039836072 23.632144366021787 30-31 23.86272215112457 24.23035885489663 28.310307738383322 23.59661125559548 32-33 23.623959082190456 24.32089510475749 27.78652336987705 24.268622443175005 34-35 24.171161603085686 24.248697334524742 28.32900290205518 23.2511381603344 36-37 24.09978174626358 24.23686526383242 27.72634831180827 23.937004678095725 38-39 24.1759522387766 23.845718161798032 27.946311671747363 24.032017927678005 40-41 23.92487742060859 24.215611814605474 28.028804081449415 23.830706683336523 42-43 24.649308863168006 24.080397813403817 27.51408440743403 23.756208915994144 44-45 24.240006267837387 24.536996721930887 26.91696074828335 24.30603626194837 46-47 23.930959498526832 24.49130398151639 27.15790464113588 24.4198318788209 48-49 24.78685361010375 24.883637980454946 26.18986414642741 24.139644263013892 50-51 24.102026395849183 25.21342312751639 25.902727341492376 24.78182313514205 52-53 24.166172535299182 25.006607892400268 25.818231731683532 25.00898784061702 54-55 24.27027671917097 25.063542032447177 25.88656586447605 24.7796153839058 56-57 24.3798617134485 24.627519751883188 25.650905666708155 25.341712867960155 58-59 23.958479517418787 24.64253186633696 26.275043985907292 25.123944630336965 60-61 24.192267458915225 24.777678220564802 26.080097706829086 24.949956613690887 62-63 23.691968889771715 24.88082140645438 25.8989944584829 25.528215245291 64-65 23.65793631221907 24.572080617973555 26.082182463567495 25.68780060623988 66-67 24.131618872029758 24.74475259349273 25.93827890109336 25.185349633384153 68-69 24.167356419783736 24.321858248093296 25.988107854735688 25.52267747738728 70-71 23.591024943660678 24.33821243606959 26.198995193669212 25.871767426600524 72-73 24.189881365657108 24.43086448527725 26.164693322444222 25.214560826621423 74-75 24.338975295940134 24.53143708616195 25.908348190043718 25.221239427854197 76-77 24.008685871437542 24.659382112541195 25.97042966282903 25.36150235319223 78-79 23.966565234586565 24.719394169991276 25.94167728166371 25.372363313758445 80-81 23.563661351880913 24.71939617497042 26.2353545229838 25.48158795016487 82-83 23.8478336671609 24.69442939470103 25.786208503962577 25.671528434175496 84-85 23.732637020482887 24.894990372605683 25.42159131511252 25.950781291798915 86-87 23.549166128053262 24.665039859441887 25.706071438894178 26.07972257361067 88-89 23.5691896323018 24.297925882285657 25.82464428453793 26.308240200874618 90-91 23.753037440378904 24.454730523006447 25.67500161277299 26.117230423841665 92-93 23.596280560132364 24.72337646875343 25.431352151023006 26.248990820091205 94-95 22.966159200422414 24.750999121444682 25.97787708417047 26.30496459396243 96-97 23.44865697639594 24.6330848960749 25.53827150198704 26.379986625542116 98-99 23.24725768392751 24.641935343022432 25.621709512824943 26.489097460225118 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 139.0 1 144.0 2 447.5 3 768.5 4 564.5 5 502.5 6 1022.0 7 2789.5 8 5144.5 9 5118.5 10 3108.5 11 1933.5 12 1923.0 13 2207.0 14 2586.0 15 3229.5 16 3996.5 17 4833.5 18 6019.0 19 7413.5 20 9102.0 21 11002.5 22 13395.0 23 16549.0 24 20256.0 25 24654.0 26 29765.5 27 35327.0 28 43037.5 29 53924.0 30 63249.0 31 73200.0 32 84847.0 33 95355.5 34 103987.0 35 114593.0 36 125331.0 37 138712.5 38 152155.0 39 151612.5 40 154354.0 41 164360.0 42 173546.5 43 197446.5 44 226681.5 45 256953.0 46 302055.5 47 338078.5 48 354143.0 49 374256.0 50 405514.0 51 399579.5 52 370675.0 53 355975.0 54 332422.5 55 317761.5 56 298747.5 57 275745.0 58 262831.5 59 233792.0 60 192151.5 61 146720.5 62 118968.5 63 97304.0 64 71880.5 65 56583.0 66 42771.5 67 32594.5 68 25647.0 69 21292.0 70 19319.0 71 17861.0 72 18965.5 73 16615.5 74 11179.0 75 9242.0 76 7884.5 77 5949.0 78 3583.0 79 1948.0 80 1000.0 81 659.5 82 527.0 83 398.5 84 239.0 85 174.5 86 86.5 87 38.0 88 27.5 89 18.5 90 12.5 91 5.0 92 2.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05547051852635372 2 0.007896246761401129 3 0.0 4 0.0 5 0.002521387205743351 6 0.0 7 0.0 8 0.0 9 0.004624593118826829 10-11 0.0 12-13 0.0 14-15 0.002625932528908319 16-17 0.004489299171201576 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 6.026730394215815E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.9679119654582254E-4 54-55 0.0 56-57 9.962554325132265E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0014820836989857258 70-71 2.7673762014256294E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 5.596249651771827E-4 80-81 9.409079084847139E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 4.735288166883854E-4 92-93 9.962554325132265E-4 94-95 6.395713887739231E-4 96-97 2.70587895250506E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 8130445.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.47280297293157 #Duplication Level Percentage of deduplicated Percentage of total 1 70.48377102080161 23.592893801685165 2 15.663630799451209 10.48611255181546 3 5.580012408801663 5.603359678389942 4 2.5611402651070514 3.429141739198803 5 1.4039077168019987 2.349636319834576 6 0.8380053569144181 1.6830232921354513 7 0.5483158965363799 1.2847568980182005 8 0.3712079958113544 0.9940297684616218 9 0.3057440250619943 0.9210698559946069 >10 1.8023100373216663 11.56851897765761 >50 0.20593430598311685 4.8627422187149065 >100 0.1884178530076054 13.331303886793435 >500 0.028658297348073153 6.652868619332156 >1k 0.01823687684782564 11.191172795907747 >5k 5.210536240301432E-4 0.9801912416067036 >10k+ 1.860905800107654E-4 1.0691783544536366 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24632 0.3029600470822938 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 16571 0.20381418237255156 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15915 0.19574574331417283 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 15318 0.18840297179305684 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13387 0.16465273425993288 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.2299449784113907E-5 0.0 2 0.0 0.0 0.0 3.6898349352341724E-5 0.0 3 0.0 0.0 0.0 3.6898349352341724E-5 0.0 4 0.0 0.0 0.0 4.919779913645563E-5 0.0 5 0.0 0.0 0.0 7.379669870468345E-5 1.2299449784113907E-5 6 0.0 0.0 0.0 7.379669870468345E-5 1.2299449784113907E-5 7 0.0 0.0 0.0 7.379669870468345E-5 1.2299449784113907E-5 8 0.0 0.0 0.0 7.379669870468345E-5 1.2299449784113907E-5 9 0.0 1.2299449784113907E-5 0.0 1.1069504805702517E-4 1.2299449784113907E-5 10-11 0.0 1.2299449784113907E-5 0.0 1.35293947625253E-4 1.2299449784113907E-5 12-13 0.0 1.2299449784113907E-5 0.0 1.6604257208553778E-4 6.149724892056954E-5 14-15 6.1497248920569535E-6 1.2299449784113907E-5 0.0 1.7219229697759472E-4 8.609614848879735E-5 16-17 2.4598899568227814E-5 1.2299449784113907E-5 0.0 1.967911965458225E-4 1.1684477294908212E-4 18-19 2.4598899568227814E-5 1.844917467617086E-5 0.0 2.4598899568227815E-4 1.2299449784113908E-4 20-21 2.4598899568227814E-5 2.4598899568227814E-5 0.0 4.919779913645563E-4 1.4144367251730996E-4 22-23 2.4598899568227814E-5 2.4598899568227814E-5 0.0 0.0017403721444521178 1.598928471934808E-4 24-25 3.074862446028477E-5 5.534752402851258E-5 0.0 0.0040280698042973045 1.7219229697759472E-4 26-27 6.76469738126265E-5 7.379669870468345E-5 0.0 0.006506408935796257 1.967911965458225E-4 28-29 7.379669870468345E-5 7.379669870468345E-5 0.0 0.017575913741498774 2.0909064632993642E-4 30-31 7.379669870468345E-5 1.1069504805702517E-4 0.0 0.04835528682624383 2.0909064632993642E-4 32-33 7.379669870468345E-5 1.1069504805702517E-4 0.0 0.10252206367548147 2.0909064632993642E-4 34-35 7.379669870468345E-5 1.1069504805702517E-4 0.0 0.17257357992090222 2.3368954589816424E-4 36-37 7.379669870468345E-5 1.1069504805702517E-4 0.0 0.2791421625753572 2.3368954589816424E-4 38-39 7.379669870468345E-5 1.1069504805702517E-4 0.0 0.4450186920888094 2.3983927079022118E-4 40-41 7.379669870468345E-5 1.1069504805702517E-4 0.0 0.6433165712331859 2.4598899568227815E-4 42-43 7.379669870468345E-5 1.2299449784113908E-4 0.0 0.8233374679983692 2.5213872057433507E-4 44-45 7.379669870468345E-5 1.35293947625253E-4 0.0 1.0258233097942364 2.64438170358449E-4 46-47 7.379669870468345E-5 1.35293947625253E-4 0.0 1.2521454852717164 2.70587895250506E-4 48-49 7.379669870468345E-5 1.35293947625253E-4 0.0 1.495594644573575 2.70587895250506E-4 50-51 7.379669870468345E-5 1.475933974093669E-4 0.0 1.7675047306758733 2.8288734503461986E-4 52-53 7.99464235967404E-5 1.475933974093669E-4 0.0 2.035294501100493 3.074862446028477E-4 54-55 8.609614848879736E-5 1.475933974093669E-4 0.0 2.3010979103849794 3.074862446028477E-4 56-57 8.609614848879736E-5 1.475933974093669E-4 0.0 2.600097534636788 3.259354192790186E-4 58-59 8.609614848879736E-5 1.475933974093669E-4 0.0 2.931659952192039 3.566840437393033E-4 60-61 8.609614848879736E-5 1.475933974093669E-4 0.0 3.268689475176328 3.6898349352341726E-4 62-63 8.609614848879736E-5 1.475933974093669E-4 0.0 3.613117117205762 3.6898349352341726E-4 64-65 8.609614848879736E-5 1.7219229697759472E-4 0.0 3.967950093752556 3.751332184154742E-4 66-67 8.609614848879736E-5 1.7219229697759472E-4 0.0 4.350998008104108 3.8128294330753114E-4 68-69 8.609614848879736E-5 1.7219229697759472E-4 0.0 4.766842405304999 3.8128294330753114E-4 70-71 8.609614848879736E-5 1.7219229697759472E-4 0.0 5.238766143796557 3.8128294330753114E-4 72-73 8.609614848879736E-5 1.7219229697759472E-4 0.0 5.702971977548584 4.181812926598729E-4 74-75 8.609614848879736E-5 1.7834202186965166E-4 0.0 6.1532302352454264 4.366304673360437E-4 76-77 8.609614848879736E-5 2.1524037122199336E-4 0.0 6.632638188930619 4.4278019222810066E-4 78-79 9.839559827291126E-5 2.2139009611405033E-4 0.0 7.132899859724775 4.4278019222810066E-4 80-81 9.839559827291126E-5 2.2139009611405033E-4 0.0 7.680612807786044 4.673790917963285E-4 82-83 9.839559827291126E-5 2.2139009611405033E-4 0.0 8.260169031338384 4.981277162566133E-4 84-85 9.839559827291126E-5 2.3368954589816424E-4 0.0 8.826958426014812 5.042774411486702E-4 86-87 9.839559827291126E-5 2.3368954589816424E-4 0.0 9.40955876338872 5.22726615824841E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 595 0.0 31.262802 2 GTATCAA 49410 0.0 31.151098 1 ATCAACG 62325 0.0 24.35309 3 CAACGCA 62325 0.0 24.323246 5 AACGCAG 64955 0.0 23.659836 6 TATCAAC 65955 0.0 23.450895 2 TCAACGC 65000 0.0 23.386639 4 GGTATCA 33185 0.0 22.938456 1 AGTACTT 36215 0.0 21.957718 12-13 ACGCAGA 69280 0.0 21.692837 7 CGCAGAG 69865 0.0 21.465134 8 GTACTAG 2970 0.0 20.992834 1 TATTCGC 520 0.0 20.568064 9 GCAGAGT 73835 0.0 20.185274 9 GAGTACT 34815 0.0 19.447983 12-13 TACTTTT 42520 0.0 18.914791 14-15 AGAGTAC 72370 0.0 18.277925 10-11 TAGTACT 3720 0.0 18.125555 4 CTAGGCA 4870 0.0 17.951267 4 TATATAC 3850 0.0 17.875872 3 >>END_MODULE