##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765522_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11743319
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.358117751889395	34.0	31.0	34.0	31.0	34.0
2	32.388388240155955	34.0	31.0	34.0	31.0	34.0
3	32.4168729470774	34.0	31.0	34.0	31.0	34.0
4	35.86405555363011	37.0	35.0	37.0	35.0	37.0
5	35.88200252415863	37.0	37.0	37.0	35.0	37.0
6	35.97811410896698	37.0	37.0	37.0	35.0	37.0
7	35.932933100088654	37.0	37.0	37.0	35.0	37.0
8	35.96461383702512	37.0	37.0	37.0	35.0	37.0
9	37.68304429097089	39.0	38.0	39.0	35.0	39.0
10-11	37.619104232798236	39.0	38.0	39.0	35.0	39.0
12-13	37.59236145249908	39.0	38.0	39.0	35.0	39.0
14-15	38.97969952106385	41.0	39.0	41.0	36.0	41.0
16-17	38.944882745670114	41.0	39.0	41.0	36.0	41.0
18-19	38.955951677715646	41.0	39.0	41.0	36.0	41.0
20-21	38.934550871010146	41.0	39.0	41.0	35.5	41.0
22-23	38.84573224145576	41.0	39.0	41.0	35.0	41.0
24-25	38.76982840200458	41.0	39.0	41.0	35.0	41.0
26-27	38.63526159001557	41.0	38.0	41.0	35.0	41.0
28-29	38.488912546785116	40.0	38.0	41.0	34.5	41.0
30-31	38.24224961444035	40.0	38.0	41.0	34.0	41.0
32-33	37.96311685818975	40.0	38.0	41.0	34.0	41.0
34-35	37.8025265259336	40.0	38.0	41.0	33.5	41.0
36-37	37.63530565762541	40.0	37.0	41.0	33.0	41.0
38-39	37.42512700200003	40.0	37.0	41.0	33.0	41.0
40-41	37.15635507304196	40.0	37.0	41.0	32.0	41.0
42-43	36.855968274386484	40.0	36.0	41.0	31.0	41.0
44-45	36.43345497129049	40.0	36.0	41.0	30.5	41.0
46-47	35.93853454036291	39.5	35.0	41.0	29.0	41.0
48-49	35.492139317683524	39.0	35.0	41.0	26.5	41.0
50-51	34.199117770708604	38.0	34.0	40.0	24.5	40.5
52-53	34.10977424695693	38.0	34.0	39.5	23.5	40.5
54-55	34.9003005879343	39.0	35.0	40.5	24.5	41.0
56-57	34.89306779454769	39.0	35.0	41.0	25.0	41.0
58-59	34.672073670143845	38.5	35.0	41.0	24.0	41.0
60-61	34.32792615954655	38.0	35.0	40.5	23.0	41.0
62-63	33.94830171095582	37.0	35.0	40.0	22.0	41.0
64-65	33.64200252926791	37.0	34.0	40.0	21.5	41.0
66-67	33.317298073909086	36.0	34.0	39.0	20.0	41.0
68-69	33.063859203688494	36.0	34.0	39.0	19.5	41.0
70-71	32.709461567040805	35.0	34.0	38.5	18.0	41.0
72-73	32.33253563153654	35.0	34.0	37.0	15.0	39.5
74-75	31.955426740940958	35.0	34.0	37.0	9.0	39.0
76-77	31.578951912998363	35.0	33.5	36.5	4.5	39.0
78-79	31.231415752224734	35.0	33.0	36.0	2.0	37.5
80-81	30.906743187339117	35.0	33.0	35.5	2.0	37.0
82-83	30.615171571171658	35.0	33.0	35.0	2.0	37.0
84-85	30.327339655850274	35.0	33.0	35.0	2.0	36.0
86-87	30.06066713337175	35.0	32.5	35.0	2.0	36.0
88-89	29.804262917493766	35.0	32.0	35.0	2.0	36.0
90-91	29.54049728190131	35.0	32.0	35.0	2.0	35.5
92-93	29.228406849886305	35.0	31.0	35.0	2.0	35.0
94-95	28.872082500696777	35.0	31.0	35.0	2.0	35.0
96-97	28.540723240167452	35.0	31.0	35.0	2.0	35.0
98-99	28.214704633332367	35.0	30.0	35.0	2.0	35.0
100	27.66039089971072	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3956399125008545
1101	2	0.5004925214243414
1101	3	0.5257131607836527
1101	4	0.4052507603767026
1101	5	0.4696789261526675
1101	6	0.42273409649975946
1101	7	0.41670597598301384
1101	8	0.3744606220203508
1101	9	0.43848614444102196
1101	10-11	0.4354148573088352
1101	12-13	0.5087023220861582
1101	14-15	0.6492936921827095
1101	16-17	0.6415356935188683
1101	18-19	0.5907896307731733
1101	20-21	0.5790333929156048
1101	22-23	0.6496391956835765
1101	24-25	0.5526267250556103
1101	26-27	0.6739254720194481
1101	28-29	0.6679983822010414
1101	30-31	0.6598371637341742
1101	32-33	0.6342336476203414
1101	34-35	0.7172823708620939
1101	36-37	0.7139411897729175
1101	38-39	0.7340910657323221
1101	40-41	0.7637828868825238
1101	42-43	0.7036412143713733
1101	44-45	0.6832774280229472
1101	46-47	0.8581157011504104
1101	48-49	0.7719262200509931
1101	50-51	0.7871316255433953
1101	52-53	0.7874411497410065
1101	54-55	0.8646241402883135
1101	56-57	0.9482628050735187
1101	58-59	0.9461867066644984
1101	60-61	0.9419515914020948
1101	62-63	0.9804730969788906
1101	64-65	0.9672108618312905
1101	66-67	0.9008854668155948
1101	68-69	0.843911537203418
1101	70-71	0.8362395609671935
1101	72-73	0.8132109199347113
1101	74-75	0.7651947307204203
1101	76-77	0.7464162922520607
1101	78-79	0.7496169200934943
1101	80-81	0.7371176994754975
1101	82-83	0.7088407663985805
1101	84-85	0.7348607457291791
1101	86-87	0.6695357189331368
1101	88-89	0.5760561789725571
1101	90-91	0.5887351732704147
1101	92-93	0.6026984718094468
1101	94-95	0.5928864309027482
1101	96-97	0.5755440109543848
1101	98-99	0.557491457196722
1101	100	0.5753038716934604
1102	1	0.44568091690393175
1102	2	0.5011431544303733
1102	3	0.5839844866952291
1102	4	0.45124498345102637
1102	5	0.4854704774748839
1102	6	0.4240211249960808
1102	7	0.4529579821580896
1102	8	0.44403853870333876
1102	9	0.4291360315872481
1102	10-11	0.4982299361258242
1102	12-13	0.5053985924115096
1102	14-15	0.6815128325909114
1102	16-17	0.6714287171898192
1102	18-19	0.7003551307684361
1102	20-21	0.6445857772255081
1102	22-23	0.6937979239514647
1102	24-25	0.6427283426413126
1102	26-27	0.6351691476380452
1102	28-29	0.6320428061119827
1102	30-31	0.5649920924097742
1102	32-33	0.6169244951692789
1102	34-35	0.6481169665229061
1102	36-37	0.6577916293204424
1102	38-39	0.7051546564516755
1102	40-41	0.7053453075678533
1102	42-43	0.679076706172907
1102	44-45	0.6736352672331023
1102	46-47	0.6119235658754008
1102	48-49	0.5997590608843311
1102	50-51	0.5521627128728426
1102	52-53	0.6319601086208806
1102	54-55	0.732318412174422
1102	56-57	0.737834254060914
1102	58-59	0.7325177077997438
1102	60-61	0.7124022356049693
1102	62-63	0.6408003487824132
1102	64-65	0.6342365428397443
1102	66-67	0.6234620171621117
1102	68-69	0.5812622109723904
1102	70-71	0.534222555721314
1102	72-73	0.5072506415304616
1102	74-75	0.48313807864363056
1102	76-77	0.5167475631131104
1102	78-79	0.4728480920968181
1102	80-81	0.4783983926350359
1102	82-83	0.5114475195957198
1102	84-85	0.47106857267825575
1102	86-87	0.46982309853803983
1102	88-89	0.4367239506564644
1102	90-91	0.43412882716606305
1102	92-93	0.45605272141277453
1102	94-95	0.4124330733441788
1102	96-97	0.45825426516737267
1102	98-99	0.4608824721437905
1102	100	0.4936093998382205
1103	1	0.4838895770289682
1103	2	0.5432528483177776
1103	3	0.5670743433123491
1103	4	0.45212312152028034
1103	5	0.4808264632176886
1103	6	0.44895036035175906
1103	7	0.44527717028584846
1103	8	0.42904222657429614
1103	9	0.4910605982141405
1103	10-11	0.5284608238793851
1103	12-13	0.5299945678051117
1103	14-15	0.6346683757891967
1103	16-17	0.6510326668803046
1103	18-19	0.6009475071089057
1103	20-21	0.6440157984259329
1103	22-23	0.6505278057465063
1103	24-25	0.606762139758203
1103	26-27	0.6347972712261338
1103	28-29	0.6036396349291238
1103	30-31	0.5861759182959219
1103	32-33	0.6932031823644564
1103	34-35	0.6370348177491678
1103	36-37	0.6235755239438774
1103	38-39	0.6334554940424724
1103	40-41	0.6490541086429289
1103	42-43	0.6078378518457441
1103	44-45	0.5655748556589799
1103	46-47	0.5948512334032188
1103	48-49	0.5355302506957642
1103	50-51	0.5677270729789612
1103	52-53	0.5680568351044286
1103	54-55	0.6642040148034312
1103	56-57	0.6999893608933192
1103	58-59	0.6780526807875376
1103	60-61	0.6780007712691898
1103	62-63	0.6973008488654457
1103	64-65	0.70340130764626
1103	66-67	0.7106579608902237
1103	68-69	0.6879907411916903
1103	70-71	0.6428305485541017
1103	72-73	0.6100437693470511
1103	74-75	0.6609181734084011
1103	76-77	0.6470128195070295
1103	78-79	0.6192503905479114
1103	80-81	0.5745873352007891
1103	82-83	0.5144109459560795
1103	84-85	0.5575852530537588
1103	86-87	0.5170436795611444
1103	88-89	0.5618918356721245
1103	90-91	0.5174654428976311
1103	92-93	0.5474914544842875
1103	94-95	0.5072924515421029
1103	96-97	0.5505981678230292
1103	98-99	0.5615628939898869
1103	100	0.5350850116193264
1104	1	0.4415153033147732
1104	2	0.49971530167347566
1104	3	0.505961432120543
1104	4	0.4487811431240587
1104	5	0.44852394492816927
1104	6	0.41702724608434494
1104	7	0.4455707938389466
1104	8	0.4265788745809047
1104	9	0.49859774650291655
1104	10-11	0.5032717102063984
1104	12-13	0.5365027712637911
1104	14-15	0.6216306328503904
1104	16-17	0.5732631180428669
1104	18-19	0.5983854545867615
1104	20-21	0.5899465796344643
1104	22-23	0.5878980421368496
1104	24-25	0.6174901064197726
1104	26-27	0.6085990993288704
1104	28-29	0.605100630297386
1104	30-31	0.5979980690759774
1104	32-33	0.6473715536529951
1104	34-35	0.6185559373713119
1104	36-37	0.5713983536115137
1104	38-39	0.6539382616595404
1104	40-41	0.6640405986866114
1104	42-43	0.6781252055073637
1104	44-45	0.6683518269070916
1104	46-47	0.642960829173667
1104	48-49	0.6002026773089639
1104	50-51	0.5915191681778253
1104	52-53	0.5900079075083511
1104	54-55	0.6392176946374946
1104	56-57	0.6073551820207825
1104	58-59	0.5557605125724123
1104	60-61	0.5490969923621094
1104	62-63	0.5690115130889879
1104	64-65	0.5473864120174312
1104	66-67	0.5521822692267406
1104	68-69	0.5047495704316773
1104	70-71	0.4778830290886873
1104	72-73	0.51263325167033
1104	74-75	0.5189728020392828
1104	76-77	0.4837995959739452
1104	78-79	0.5037515242870505
1104	80-81	0.5234445956587521
1104	82-83	0.5036675032760272
1104	84-85	0.4525784571646767
1104	86-87	0.41779457036731316
1104	88-89	0.39294071853892376
1104	90-91	0.367756300092946
1104	92-93	0.3485976547258893
1104	94-95	0.33327437997868614
1104	96-97	0.3091163445080376
1104	98-99	0.2913326597721131
1104	100	0.3098766191599651
1105	1	0.33300508812904894
1105	2	0.3769035317518785
1105	3	0.3787960892638793
1105	4	0.32958398709935466
1105	5	0.3583236661835585
1105	6	0.33214591556886575
1105	7	0.34376136774661603
1105	8	0.3324165826832868
1105	9	0.3062201526743138
1105	10-11	0.403670460107989
1105	12-13	0.3768355807054604
1105	14-15	0.5026383554691378
1105	16-17	0.4954302939823805
1105	18-19	0.5010933275150364
1105	20-21	0.4860562431317206
1105	22-23	0.4822280270825061
1105	24-25	0.4673683117827778
1105	26-27	0.3960880153409221
1105	28-29	0.4667537767792993
1105	30-31	0.4824859124644192
1105	32-33	0.4757500104830612
1105	34-35	0.4846057647593227
1105	36-37	0.48825435151613306
1105	38-39	0.5042744687484557
1105	40-41	0.4783292841844684
1105	42-43	0.38884932258571325
1105	44-45	0.46661847480553575
1105	46-47	0.442854039291646
1105	48-49	0.4685423074935642
1105	50-51	0.47506751976690254
1105	52-53	0.49855647779494916
1105	54-55	0.4591376237448799
1105	56-57	0.47556915586137194
1105	58-59	0.5208509244661528
1105	60-61	0.4847404872429806
1105	62-63	0.44124687821349795
1105	64-65	0.4943442453111686
1105	66-67	0.46186184484783865
1105	68-69	0.38160351842713425
1105	70-71	0.3726682791174909
1105	72-73	0.36964689048971877
1105	74-75	0.4117508766375373
1105	76-77	0.40379761997752084
1105	78-79	0.36050951389220387
1105	80-81	0.4136934135577235
1105	82-83	0.3450217260350996
1105	84-85	0.3254212493879507
1105	86-87	0.36427354263586054
1105	88-89	0.33151378715797364
1105	90-91	0.3649844926405095
1105	92-93	0.38265999226696223
1105	94-95	0.3935921710991188
1105	96-97	0.4243721689141857
1105	98-99	0.3908476237581304
1105	100	0.4257893079994268
1106	1	0.2642488792819151
1106	2	0.29988368822416334
1106	3	0.3374883278540395
1106	4	0.34844044073030034
1106	5	0.3289999459563333
1106	6	0.29925295555356257
1106	7	0.33432270322946067
1106	8	0.3224238116058089
1106	9	0.3566310210605863
1106	10-11	0.35866486865347724
1106	12-13	0.4269333480347868
1106	14-15	0.4842023492580765
1106	16-17	0.5167816411091835
1106	18-19	0.48658857280771173
1106	20-21	0.5076305783006845
1106	22-23	0.4901835396098164
1106	24-25	0.5239903839260478
1106	26-27	0.461322112594047
1106	28-29	0.5283484796919709
1106	30-31	0.521890554358869
1106	32-33	0.5460388004360297
1106	34-35	0.4755382899368996
1106	36-37	0.502730012629705
1106	38-39	0.5111645987240578
1106	40-41	0.5301720629865372
1106	42-43	0.5700221450797613
1106	44-45	0.5932049968910107
1106	46-47	0.626655234461623
1106	48-49	0.5237518406698385
1106	50-51	0.5868394082357611
1106	52-53	0.6591823631785658
1106	54-55	0.6773202611748772
1106	56-57	0.6755755427407308
1106	58-59	0.6294273636975234
1106	60-61	0.6214166419347222
1106	62-63	0.6068872994249759
1106	64-65	0.5940759149674051
1106	66-67	0.6353773405843341
1106	68-69	0.5724650989406044
1106	70-71	0.5892347138546512
1106	72-73	0.5139991992219208
1106	74-75	0.570267844964075
1106	76-77	0.5436874556065199
1106	78-79	0.5617823976104859
1106	80-81	0.6297725027484518
1106	82-83	0.5832207345459892
1106	84-85	0.5420858139460627
1106	86-87	0.550705668489929
1106	88-89	0.5073085581539445
1106	90-91	0.5302921071341302
1106	92-93	0.5701409143249556
1106	94-95	0.5793121251979123
1106	96-97	0.5616482686443156
1106	98-99	0.5294411748215744
1106	100	0.6180503932070991
1107	1	0.2647351809368885
1107	2	0.3000456491635788
1107	3	0.31314747053779257
1107	4	0.2887695458746862
1107	5	0.3146691574749383
1107	6	0.2498290300276267
1107	7	0.2805276365590359
1107	8	0.2776054791352749
1107	9	0.2987436618493291
1107	10-11	0.30751454647782595
1107	12-13	0.32037708092934025
1107	14-15	0.3762385169046283
1107	16-17	0.4157883311559587
1107	18-19	0.38449359339707456
1107	20-21	0.38703429205504136
1107	22-23	0.3551148658518031
1107	24-25	0.3833920827535735
1107	26-27	0.37530498629595854
1107	28-29	0.4077591686886919
1107	30-31	0.40836068801417724
1107	32-33	0.4089010732606013
1107	34-35	0.39985512213428365
1107	36-37	0.4358254048200294
1107	38-39	0.43930091577289687
1107	40-41	0.3934860834363434
1107	42-43	0.4038723390398218
1107	44-45	0.3892102643105275
1107	46-47	0.41184373740642855
1107	48-49	0.42245052463330524
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2316	82-83	-0.4842961940422974
2316	84-85	-0.5503973784921996
2316	86-87	-0.567759150101363
2316	88-89	-0.5894592261928864
2316	90-91	-0.5096784229016365
2316	92-93	-0.46829888202770675
2316	94-95	-0.2539511135935193
2316	96-97	-0.5137807704654129
2316	98-99	-0.32664313227118313
2316	100	-0.3749910846077249
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33341.0
3	4825.0
4	4600.0
5	5406.0
6	6607.0
7	7918.0
8	9906.0
9	12191.0
10	16429.0
11	25061.0
12	40448.0
13	60329.0
14	63276.0
15	78873.0
16	106357.0
17	151987.0
18	154701.0
19	85032.0
20	67130.0
21	57824.0
22	57939.0
23	65508.0
24	73524.0
25	78994.0
26	86596.0
27	100498.0
28	120310.0
29	143012.0
30	174927.0
31	215431.0
32	272788.0
33	359939.0
34	516586.0
35	822608.0
36	1502716.0
37	2869525.0
38	2683101.0
39	606186.0
40	890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.969168499795025	17.81249334150393	13.652145676388141	23.566192482312903
2	14.840733479453533	21.779831825703756	37.676910850680194	25.702523844162517
3	18.61928795460613	27.514808160471116	29.680132802456004	24.185771082466754
4	13.02841892353789	18.23020197522032	35.14611563974307	33.59526346149872
5	13.411226167125989	36.63013539236413	33.376740972240555	16.581897468269325
6	30.31086326054685	34.86692575990176	18.33380206228798	16.48840891726341
7	25.221642826862013	32.749517691114654	22.062160237033286	19.966679244990047
8	27.418111306398263	31.090590987740573	21.123770850845496	20.367526855015665
9	27.8477780926448	14.536292605497305	19.64205336646178	37.97387593539611
10-11	24.545842643675424	25.94228940661233	26.324283343698784	23.187584606013463
12-13	27.119379985245263	23.975566976450683	26.6723247117502	22.232728326553854
14-15	25.2964539823792	23.089911277587806	25.88378121038226	25.729853529650732
16-17	22.39380041249216	26.51567712004172	27.437521997044417	23.653000470421706
18-19	23.586591296641366	25.2648353227448	29.743910449975324	21.40466293063851
20-21	24.101092571280727	24.62269356646595	28.97176922239677	22.304444639856552
22-23	23.02155450452687	25.511502144736582	27.267980242116472	24.198963108620077
24-25	22.674281741677937	26.287746493017515	26.929673323827856	24.108298441476688
26-27	23.443414290759815	25.037887935666465	27.289535156996724	24.22916261657699
28-29	22.83337321113699	24.45908607755138	29.436441928679198	23.27109878263243
30-31	24.131457126596914	24.51456774852972	28.5477047597956	22.80627036507777
32-33	21.796978911475446	25.635144491147	28.653279966789295	23.91459663058826
34-35	21.696028108590223	26.101905323608392	28.945675849322967	23.25639071847842
36-37	23.10525178647777	25.892940405087757	27.72433302818722	23.277474780247253
38-39	23.671591753135413	24.794703285297313	27.563926840886495	23.969778120680783
40-41	22.75029551055397	25.6115554312502	27.69325908598254	23.944889972213293
42-43	24.22053829395989	25.138016222277393	27.322522801494	23.318922682268717
44-45	23.969251112151316	24.96357776651544	27.651308580468953	23.415862540864293
46-47	22.97685069678973	25.252646217307746	28.643559571417406	23.126943514485124
48-49	23.545369985944642	25.936542728681356	27.705389516588824	22.812697768785178
50-51	22.61013098144883	26.41338253004658	26.578065647530934	24.39842084097365
52-53	22.24538808958143	26.740717094333665	25.401718211988822	25.61217660409609
54-55	22.89897976424826	26.263309588272882	25.33650436033969	25.501206287139166
56-57	23.480022450641115	25.045952963297907	25.860978079590303	25.61304650647067
58-59	23.185119793588953	24.799185673752383	27.340706147373506	24.67498838528516
60-61	23.31462063170416	25.37538510436439	26.610174462422613	24.699819801508838
62-63	21.691821612503205	25.619781924902522	27.113413683851658	25.574982778742616
64-65	21.685177864450104	25.675447219790843	27.082361622974478	25.557013292784575
66-67	23.237903255715135	25.41768864866137	26.179216388156412	25.165191707467084
68-69	23.552929602330845	24.752283912554752	26.125109916907913	25.56967656820649
70-71	22.728129844843505	25.47123240774906	26.26205368819357	25.53858405921386
72-73	24.0100782357936	25.11051108616172	26.11013855100883	24.76927212703585
74-75	24.24793917910494	24.75554074581131	26.324021780107465	24.67249829497629
76-77	23.3329184725126	25.482284234484116	27.05224098630985	24.132556306693438
78-79	23.45280433274301	25.572328063097267	26.895635861598677	24.079231742561046
80-81	22.96690848960871	25.663852372317113	26.486118285093696	24.88312085298048
82-83	23.13243643851622	25.905835808477462	25.43609744882529	25.525630304181036
84-85	23.495292776963453	25.976374613795134	25.145987764453142	25.382344844788275
86-87	23.813172689186935	24.94831776247116	25.682981483464285	25.55552806487762
88-89	24.066249066498568	24.287052276506643	26.439839857856146	25.206858799138644
90-91	24.50837614710242	24.942046427432178	25.756111201081737	24.79346622438366
92-93	23.9510638863851	25.051241021737447	25.987131426996807	25.010563664880646
94-95	23.710010109339578	25.13828283442515	26.237661227770463	24.9140458284648
96-97	24.936351418626863	24.853675994555825	25.289684481318226	24.92028810549909
98-99	25.27891322357209	24.425958276751057	25.0601486166235	25.23497988305335
100	25.113952075848474	24.714109426325102	25.431022352988368	24.740916144838053
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4257.0
1	5540.5
2	4405.5
3	1768.5
4	1389.5
5	1439.5
6	1942.5
7	3285.5
8	5176.5
9	5596.0
10	4604.0
11	4242.5
12	4792.0
13	5436.0
14	6143.5
15	7085.0
16	8098.5
17	9182.0
18	10477.0
19	12061.5
20	14080.5
21	16646.5
22	20099.0
23	24688.5
24	30030.0
25	36652.0
26	45935.5
27	56417.5
28	66187.5
29	79384.0
30	95246.5
31	109817.5
32	125053.0
33	140209.5
34	155732.0
35	166221.0
36	175715.5
37	191183.5
38	200543.0
39	206490.5
40	212816.5
41	219956.5
42	231788.5
43	255076.0
44	285057.5
45	315315.5
46	357551.0
47	413591.5
48	554534.0
49	723641.0
50	906315.5
51	862880.0
52	620032.5
53	518288.5
54	460581.5
55	404579.5
56	362737.5
57	320030.0
58	288799.0
59	261774.5
60	223094.0
61	185134.5
62	151101.0
63	119218.0
64	89327.5
65	68985.5
66	53539.0
67	40212.5
68	31622.5
69	25979.5
70	21918.5
71	18637.0
72	15434.0
73	14332.5
74	11890.5
75	9378.0
76	7391.0
77	5130.0
78	3752.5
79	2728.5
80	1995.0
81	1515.5
82	1184.5
83	908.5
84	660.5
85	431.0
86	291.5
87	210.0
88	156.0
89	125.0
90	100.5
91	86.0
92	78.0
93	71.0
94	59.0
95	44.0
96	38.5
97	33.5
98	23.5
99	15.5
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08676422738750433
2	0.2837783764538799
3	0.28864923110749185
4	0.2967900301439482
5	0.32363933909995973
6	0.17138255377376704
7	0.18815804969617192
8	0.15442823276792533
9	0.20510385522184998
10-11	0.2257453791385553
12-13	0.24215045167384108
14-15	0.1699391798860271
16-17	0.15951623216571056
18-19	0.17710921418382658
20-21	0.20859094434886763
22-23	0.17202121478604132
24-25	0.19634994161361027
26-27	0.19500023800767058
28-29	0.223739983560014
30-31	0.2576188213911246
32-33	0.2266395045557393
34-35	0.15272939447527567
36-37	0.14682816672186116
38-39	0.11416278481407173
40-41	0.10517043776124961
42-43	0.1214562935742442
44-45	0.13569843414796107
46-47	0.20071838293756644
48-49	0.20692191023679082
50-51	0.2133681287206794
52-53	0.14080772224615545
54-55	0.08516757485681858
56-57	0.12328712180943054
58-59	0.14318779895189768
60-61	0.11948070217627571
62-63	0.1407864335457463
64-65	0.11557209678115701
66-67	0.11979577494233104
68-69	0.10472763279273942
70-71	0.13046992932747548
72-73	0.15541602846690958
74-75	0.1942168138326141
76-77	0.17466952911693873
78-79	0.19964969017702747
80-81	0.10284571167657117
82-83	0.1438903260653994
84-85	0.17533373656970402
86-87	0.18882225714893722
88-89	0.17017335559052768
90-91	0.1733538874316537
92-93	0.12292095616239326
94-95	0.36405806569675914
96-97	0.363985684115368
98-99	0.41154038308931234
100	0.4446783741461847
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1743319E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.797090963596474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.07026072258533	26.872969517472907
2	13.21637592885877	9.462156226688883
3	4.45889806281155	4.788467386556074
4	2.020208394402019	2.8927033463932097
5	1.146617307372076	2.0522782026216126
6	0.7523018628555155	1.6158130930033179
7	0.5156001902288702	1.2919890837329358
8	0.3726595530588475	1.0672102335440616
9	0.28453545099452343	0.9166987279457011
>10	1.819391405021085	12.391335818729395
>50	0.17420704749641808	4.3557153235964545
>100	0.14120669021597992	10.265787843269754
>500	0.016774956463395652	4.075056536517054
>1k	0.00948166443243724	6.2938917882642995
>5k	5.970819367898833E-4	1.4957468218696284
>10k+	8.836812664490272E-4	10.162180049794566
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	137218	1.1684771570967287	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	127334	1.0843101511591398	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	68561	0.5838298355005088	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	53992	0.4597678049961855	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53360	0.4543860215327541	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	53053	0.45177176912251127	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	48499	0.41299227245721587	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	46627	0.3970512935908494	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	44653	0.38024173574778986	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	44066	0.375243148891723	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	42549	0.3623251654834549	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	40720	0.3467503522641257	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34472	0.2935456322015948	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	33543	0.28563475112955716	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	25431	0.21655717604196906	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25207	0.21464970848530984	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	21825	0.18585035457182078	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	20014	0.1704288199954374	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	18443	0.15705100065833177	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	18309	0.1559099263164017	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	18201	0.15499025445872672	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	16865	0.14361357296008054	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	16464	0.14019886541445395	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	15836	0.13485114387167715	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	14996	0.12769814053420503	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	14900	0.12688065443849392	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	13635	0.11610857203146743	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	13335	0.11355392798237023	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	12998	0.11068421116721772	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	12875	0.10963680710708787	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	12691	0.10806995875697492	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	12236	0.10419541528251086	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	12027	0.10241567992830647	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	11810	0.10056782073279283	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	11774	0.10026126344690117	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0218576196388772E-4	0.0	0.0	0.0	0.0
2	1.8734056360046082E-4	0.0	0.0	8.51548016365731E-6	0.0
3	2.2140248425509007E-4	0.0	0.0	1.703096032731462E-5	0.0
4	2.724953652370339E-4	0.0	0.0	2.554644049097193E-5	0.0
5	0.001617941231094889	1.703096032731462E-5	0.0	5.109288098194386E-5	0.0
6	0.0032018205415351485	1.703096032731462E-5	0.0	5.109288098194386E-5	0.0
7	0.003533924267917784	1.703096032731462E-5	0.0	8.51548016365731E-5	0.0
8	0.003900089914955048	1.703096032731462E-5	0.0	9.367028180023041E-5	0.0
9	0.004223678161174026	1.703096032731462E-5	0.0	1.1070124212754503E-4	0.0
10-11	0.007276477799845171	1.703096032731462E-5	0.0	1.9585604376411815E-4	8.51548016365731E-6
12-13	0.015144781471064525	1.703096032731462E-5	0.0	2.8526858548251987E-4	3.8319660736457894E-5
14-15	0.024452201289941966	1.703096032731462E-5	0.0	3.321037263826351E-4	5.109288098194386E-5
16-17	0.028471507927188215	1.703096032731462E-5	0.0	4.726091490829807E-4	5.960836114560117E-5
18-19	0.030813264972193978	3.406192065462924E-5	0.0	6.982693734198994E-4	5.960836114560117E-5
20-21	0.03970768400313404	4.257740081828655E-5	0.0	0.0013156416852850546	6.812384130925848E-5
22-23	0.04330973211236108	4.257740081828655E-5	0.0	0.004138523359537453	6.812384130925848E-5
24-25	0.06706792176896498	5.109288098194386E-5	0.0	0.010844463988417584	7.238158139108713E-5
26-27	0.14435867747440057	5.960836114560117E-5	0.0	0.021880526280517457	7.663932147291579E-5
28-29	0.1598611091123387	5.960836114560117E-5	0.0	0.06737022131477481	7.663932147291579E-5
30-31	0.16240723768127222	6.812384130925848E-5	0.0	0.19901102916475316	7.663932147291579E-5
32-33	0.16419123077555842	6.812384130925848E-5	0.0	0.38403963990078105	7.663932147291579E-5
34-35	0.1656729243240348	7.663932147291579E-5	0.0	0.6129187157395621	7.663932147291579E-5
36-37	0.16804874328969518	8.51548016365731E-5	0.0	0.9572293829368	7.663932147291579E-5
38-39	0.17066725344001982	8.51548016365731E-5	0.0	1.4435441973431873	7.663932147291579E-5
40-41	0.17284295862183424	8.51548016365731E-5	0.0	2.1069554527131555	7.663932147291579E-5
42-43	0.17823751530551116	8.51548016365731E-5	0.0	2.591141397078628	7.663932147291579E-5
44-45	0.18478591955136364	8.51548016365731E-5	0.0	3.0692132266865952	8.089706155474445E-5
46-47	0.18816230743625376	8.51548016365731E-5	0.0	3.6139144308350986	8.941254171840175E-5
48-49	0.19049980674117767	8.51548016365731E-5	0.0	4.115152624228295	9.367028180023041E-5
50-51	0.19364627666164907	8.51548016365731E-5	0.0	4.669833971128606	1.1921672229120234E-4
52-53	0.19503429992832522	8.51548016365731E-5	0.0	5.293018098205456	1.3624768261851695E-4
54-55	0.19791253222364139	9.367028180023041E-5	0.0	5.821642075804975	1.5327864294583158E-4
56-57	0.20493354561857685	9.367028180023041E-5	0.0	6.360774155926446	1.5327864294583158E-4
58-59	0.20722846752268248	9.367028180023041E-5	0.0	6.911351041387873	1.5327864294583158E-4
60-61	0.20850153180714925	9.367028180023041E-5	0.0	7.411882449927486	1.5327864294583158E-4
62-63	0.20954042038711543	9.367028180023041E-5	0.0	7.932084617645147	1.617941231094889E-4
64-65	0.21030681360184458	9.367028180023041E-5	0.0	8.476377078745795	1.703096032731462E-4
66-67	0.21130738252107434	9.367028180023041E-5	0.0	9.008130495305458	1.7456734335497486E-4
68-69	0.2125506426249683	9.367028180023041E-5	0.0	9.604056570378443	1.7882508343680352E-4
70-71	0.21398550103254455	9.367028180023041E-5	0.0	10.313702625296987	1.7882508343680352E-4
72-73	0.21737891987776198	9.367028180023041E-5	0.0	10.905358187067897	1.7882508343680352E-4
74-75	0.22058074041929715	9.367028180023041E-5	0.0	11.463105106827124	1.7882508343680352E-4
76-77	0.2215727938583632	9.367028180023041E-5	0.0	12.052218797769182	1.7882508343680352E-4
78-79	0.22233918707309236	1.0218576196388772E-4	0.0	12.627528895365952	1.9159830368228948E-4
80-81	0.2238762312426325	1.1070124212754503E-4	0.0	13.257244395728328	2.0437152392777544E-4
82-83	0.224485088074334	1.1070124212754503E-4	0.0	13.975129177705213	2.384334445824047E-4
84-85	0.22691625766105816	1.1921672229120234E-4	0.0	14.635385447674546	2.384334445824047E-4
86-87	0.2312165751437051	1.23474462373031E-4	0.0	15.275787875642312	2.512066648278907E-4
88	0.23211495830097095	1.2773220245485966E-4	0.0	15.779116619415687	2.6397988507337664E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	14145	0.0	27.427134	1
ATCTACA	16210	0.0	24.410664	2
TCTACAC	18490	0.0	23.7882	3
TACACTC	19175	0.0	22.057753	5
AGTACTT	57910	0.0	21.693647	12-13
CTACACT	21105	0.0	21.53512	4
GAGTACT	56350	0.0	20.70118	12-13
GTATAAG	3540	0.0	19.152058	1
TACTTTT	67855	0.0	18.682108	14-15
CACTCTT	23180	0.0	18.292068	7
GTACTTT	64070	0.0	18.275074	14-15
ACACTCT	23680	0.0	18.06449	6
ACTCTTT	25975	0.0	17.246742	8
ACTTTTT	71065	0.0	16.667519	16-17
CATGGGG	25440	0.0	16.108553	4
TATCACG	705	0.0	16.017279	2
GTACTAG	2860	0.0	15.803795	1
ATGTAAG	4265	0.0	15.665198	2
GTATTAG	3400	0.0	15.647887	1
AGTACTC	4265	0.0	15.443507	5
>>END_MODULE