FastQCFastQC Report
Fri 27 May 2016
ERR765519_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765519_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7053372
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA369980.5245434382306788No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT340480.4827194709140536No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG148370.21035328917856597No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG117540.1666436989286826No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC105040.14892167887926513No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT102780.14571753765433043No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG100770.1428678368303841No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC96900.13738109942308444No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC80590.1142574076626045No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA78600.11143606207073722No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC70650.10016485731930769No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA23400.034.1797561
TACATGA33650.029.986862
GTACTAG31100.027.0629141
GTACAAG42400.026.3209611
GTACAAA50750.024.6474841
TAGGCAT40750.022.021615
ACGTTAA7050.020.4440698
TAGTACT37450.020.361584
TATTCGC4650.019.998749
TACATAG21500.019.897832
TACAAGA32800.019.8477172
CTAGGCA48100.019.5265584
CGTTAAC8000.019.18141
GTATAAG32750.018.8841171
CTAGTAC42000.018.489433
GTGATCG24250.018.022338
CAACACA122300.017.8686125
GGCGTCG24500.017.8384288
GTCCTAC29900.017.7292371
CTGTGCG42350.017.6765739
Produced by FastQC (version 0.11.5)