##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765519_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7053372 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.88663351372932 33.0 33.0 33.0 27.0 33.0 2 30.77369504968687 33.0 33.0 33.0 27.0 33.0 3 30.7963836304111 33.0 33.0 33.0 27.0 33.0 4 34.190595363465874 37.0 37.0 37.0 27.0 37.0 5 34.45664981231672 37.0 37.0 37.0 27.0 37.0 6 34.29281271425922 37.0 37.0 37.0 27.0 37.0 7 34.22573245817745 37.0 37.0 37.0 27.0 37.0 8 34.070050324865896 37.0 37.0 37.0 27.0 37.0 9 33.83809871363654 37.0 37.0 37.0 27.0 37.0 10-11 33.98811844887807 37.0 37.0 37.0 27.0 37.0 12-13 33.905208671823914 37.0 37.0 37.0 27.0 37.0 14-15 35.24977322903145 37.0 37.0 40.0 27.0 40.0 16-17 35.39014913717864 37.0 37.0 40.0 27.0 40.0 18-19 35.447409692839116 37.0 37.0 40.0 27.0 40.0 20-21 35.49810601227328 37.0 37.0 40.0 27.0 40.0 22-23 35.5865613638413 37.0 37.0 40.0 27.0 40.0 24-25 35.73015948967387 37.0 37.0 40.0 27.0 40.0 26-27 35.71992885105167 37.0 33.0 40.0 27.0 40.0 28-29 35.73746301768856 37.0 33.0 40.0 27.0 40.0 30-31 35.63989670472506 37.0 33.0 40.0 27.0 40.0 32-33 35.514387728309245 37.0 33.0 40.0 27.0 40.0 34-35 35.3451632070448 37.0 33.0 40.0 27.0 40.0 36-37 35.11437026148628 37.0 33.0 40.0 27.0 40.0 38-39 34.77725646683601 37.0 33.0 40.0 24.5 40.0 40-41 34.84429234697957 37.0 33.0 40.0 27.0 40.0 42-43 34.73447444711551 37.0 33.0 40.0 27.0 40.0 44-45 34.6366647044846 37.0 33.0 40.0 22.0 40.0 46-47 34.545413314369355 37.0 33.0 40.0 22.0 40.0 48-49 34.4041408846719 37.0 33.0 40.0 22.0 40.0 50-51 34.2204291791217 37.0 33.0 40.0 22.0 40.0 52-53 34.07458453913958 37.0 33.0 40.0 22.0 40.0 54-55 33.8713557855732 37.0 33.0 40.0 22.0 40.0 56-57 33.651308976756084 37.0 33.0 37.0 22.0 40.0 58-59 33.425418296383626 37.0 33.0 37.0 22.0 40.0 60-61 33.195775297262074 37.0 33.0 37.0 22.0 40.0 62-63 32.93491623580891 37.0 33.0 37.0 22.0 40.0 64-65 32.70686155501227 37.0 33.0 37.0 22.0 40.0 66-67 32.473589298848836 37.0 33.0 37.0 22.0 40.0 68-69 32.24774356151923 37.0 33.0 37.0 22.0 38.5 70-71 31.96227712078705 33.0 33.0 37.0 22.0 37.0 72-73 31.597502939019805 33.0 27.0 37.0 22.0 37.0 74-75 31.34362500659259 33.0 27.0 37.0 18.5 37.0 76-77 31.126597604663417 33.0 27.0 37.0 15.0 37.0 78-79 30.858125092508946 33.0 27.0 37.0 15.0 37.0 80-81 30.640964137436676 33.0 27.0 37.0 15.0 37.0 82-83 30.49392134712305 33.0 27.0 37.0 15.0 37.0 84-85 30.32653261447149 33.0 27.0 37.0 15.0 37.0 86-87 30.029964320611477 33.0 27.0 37.0 15.0 37.0 88-89 29.858967101125533 33.0 27.0 37.0 15.0 37.0 90-91 29.6866474645035 33.0 27.0 37.0 15.0 37.0 92-93 29.399416761231365 33.0 27.0 37.0 15.0 37.0 94-95 29.245406239738948 33.0 27.0 37.0 15.0 37.0 96-97 29.039872702021103 33.0 27.0 37.0 15.0 37.0 98-99 27.99625235986419 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38883077396903687 1101 2 0.445480005053291 1101 3 0.394875463188729 1101 4 0.46012329676332797 1101 5 0.3260451254349519 1101 6 0.4243487663330896 1101 7 0.36899954165994586 1101 8 0.38723892094727574 1101 9 0.3593890453150834 1101 10-11 0.34213703429656306 1101 12-13 0.4101039196388143 1101 14-15 0.5550176587570874 1101 16-17 0.4965725486515282 1101 18-19 0.4418730937126014 1101 20-21 0.4229299322249247 1101 22-23 0.4177345645042365 1101 24-25 0.3429635112178673 1101 26-27 0.4424690761379537 1101 28-29 0.40015149072043243 1101 30-31 0.5710830788803634 1101 32-33 0.7169926137762985 1101 34-35 0.7346704634473085 1101 36-37 0.8014853228692758 1101 38-39 0.8514680013564231 1101 40-41 0.6269889969691462 1101 42-43 0.454330418842126 1101 44-45 0.41727807511711745 1101 46-47 0.40792666744843586 1101 48-49 0.29049774641381987 1101 50-51 0.23983881109953842 1101 52-53 0.36435082267459507 1101 54-55 0.2166771947756061 1101 56-57 0.30949385156986864 1101 58-59 0.2576399980547208 1101 60-61 0.09783458843745052 1101 62-63 0.27247708738453014 1101 64-65 0.3873400913930638 1101 66-67 0.3401326895821697 1101 68-69 0.33207627703419007 1101 70-71 0.3333686789305723 1101 72-73 0.20002942321355732 1101 74-75 0.33474459831395365 1101 76-77 0.26628713455984254 1101 78-79 0.24053110585239068 1101 80-81 0.24652357429713945 1101 82-83 0.34492705740199625 1101 84-85 0.28660307505638016 1101 86-87 0.23612046015529842 1101 88-89 0.1423294692671604 1101 90-91 0.07571990674732021 1101 92-93 0.20116582884221756 1101 94-95 0.22749214719258148 1101 96-97 0.1078115691661985 1101 98-99 0.08370016599268482 1102 1 0.24754264059428976 1102 2 0.3641199757173261 1102 3 0.24125674924054863 1102 4 0.2727327097542869 1102 5 0.26119150918548684 1102 6 0.36254531677842294 1102 7 0.38234163549526556 1102 8 0.3657384432205717 1102 9 0.2048408660944787 1102 10-11 0.23336273615433356 1102 12-13 0.3035846695613742 1102 14-15 0.38567940704588466 1102 16-17 0.34970667886349815 1102 18-19 0.3331249404192249 1102 20-21 0.3311459037392126 1102 22-23 0.2943507988514895 1102 24-25 0.19200473899824289 1102 26-27 0.28844181478449116 1102 28-29 0.22777166487566092 1102 30-31 0.42735357901202065 1102 32-33 0.4909750342288888 1102 34-35 0.5616435984889847 1102 36-37 0.7263171137445923 1102 38-39 0.6796509009578102 1102 40-41 0.43658222395686863 1102 42-43 0.2661806371836235 1102 44-45 0.23302666549826512 1102 46-47 0.17093073646273638 1102 48-49 0.1092052092059248 1102 50-51 0.08860162019487916 1102 52-53 0.23046932035351375 1102 54-55 0.10600089134182866 1102 56-57 0.20811550086950348 1102 58-59 0.037613976967399765 1102 60-61 0.009399379716214185 1102 62-63 0.13549403762869616 1102 64-65 0.17176857672843937 1102 66-67 0.1437499491979466 1102 68-69 0.2519277092775809 1102 70-71 0.31002130203577494 1102 72-73 0.058735115194764376 1102 74-75 0.16849592982512362 1102 76-77 0.1635472412655723 1102 78-79 0.1551511996611339 1102 80-81 0.06434855552962304 1102 82-83 0.0726639506879252 1102 84-85 0.23721362421767012 1102 86-87 0.18876410153999856 1102 88-89 0.08667115621179633 1102 90-91 0.09764167072070506 1102 92-93 0.07748126981532621 1102 94-95 0.14381270841874283 1102 96-97 -0.0683441970343246 1102 98-99 0.06278872871071428 1103 1 0.21732929244507915 1103 2 0.29129478169996403 1103 3 0.17936058196410443 1103 4 0.16588416351717683 1103 5 0.08777055216526009 1103 6 0.25864930768935324 1103 7 0.17518916442055854 1103 8 0.16801176034365284 1103 9 0.0966774831677597 1103 10-11 0.18501678743206895 1103 12-13 0.22602354808640257 1103 14-15 0.3583123516469513 1103 16-17 0.12825453884582316 1103 18-19 0.11794951628736783 1103 20-21 0.11327774111239108 1103 22-23 0.12037436538506796 1103 24-25 0.05030386885922411 1103 26-27 0.22103733769380085 1103 28-29 0.12453570588549923 1103 30-31 0.36245599978407483 1103 32-33 0.46801169251799735 1103 34-35 0.47269943522098856 1103 36-37 0.6158373507918355 1103 38-39 0.6004784450566376 1103 40-41 0.36713903232631395 1103 42-43 0.24262310535070952 1103 44-45 0.16253273833035564 1103 46-47 0.13375288531578633 1103 48-49 0.11860847540759778 1103 50-51 0.02086364211151448 1103 52-53 0.1384801691051436 1103 54-55 0.09968689888543025 1103 56-57 0.1459477603751651 1103 58-59 0.13735709131783835 1103 60-61 -0.06552794221232006 1103 62-63 0.07332014314990687 1103 64-65 0.20029482061995907 1103 66-67 0.13692281759240643 1103 68-69 0.2710577137949386 1103 70-71 0.30278888911604795 1103 72-73 0.15192639616963888 1103 74-75 0.15818809844171966 1103 76-77 0.09080069129020885 1103 78-79 0.09233809751788868 1103 80-81 0.028161897034646444 1103 82-83 0.13753523000809764 1103 84-85 0.2019280968400281 1103 86-87 0.10911892176866544 1103 88-89 0.1525827895267895 1103 90-91 -0.004484380143775724 1103 92-93 0.0042969141007773715 1103 94-95 0.13676078445705642 1103 96-97 0.024576621613828564 1103 98-99 5.92479751151842E-4 1104 1 0.18004741327727913 1104 2 0.1630335404345047 1104 3 0.11826145625008166 1104 4 0.09405013909928073 1104 5 0.05242032235980787 1104 6 0.08589110201693018 1104 7 0.04617576527081724 1104 8 0.11541749967561543 1104 9 0.09187133178907203 1104 10-11 0.06281620664221066 1104 12-13 0.09952405618280125 1104 14-15 0.1517034732600493 1104 16-17 0.018711966577455996 1104 18-19 0.1227160076736169 1104 20-21 0.11113972159213859 1104 22-23 0.045616292208137565 1104 24-25 0.08411924913466606 1104 26-27 0.1790400827250238 1104 28-29 0.08178572372944615 1104 30-31 0.2983549698689103 1104 32-33 0.3799702249755441 1104 34-35 0.4965975097531228 1104 36-37 0.5120838423644258 1104 38-39 0.43250677371207047 1104 40-41 0.2230932183685539 1104 42-43 0.22185171667245385 1104 44-45 0.10947013252474136 1104 46-47 0.08923204495018666 1104 48-49 -0.07356272726241286 1104 50-51 -0.023514611759232196 1104 52-53 0.1030792626426873 1104 54-55 -0.03703222471964551 1104 56-57 0.07743015180786728 1104 58-59 0.04512166866400236 1104 60-61 -0.05398435948765723 1104 62-63 -0.0024627492729010214 1104 64-65 0.08807448180071731 1104 66-67 0.0847745254126977 1104 68-69 0.1442084091111795 1104 70-71 0.14032484603161421 1104 72-73 -0.04850803937019066 1104 74-75 -0.029200541983836814 1104 76-77 0.030807751690176133 1104 78-79 0.082590713504203 1104 80-81 -0.10102093711239135 1104 82-83 -1.6405949830300415E-4 1104 84-85 0.047343080991847586 1104 86-87 -0.04834513313640798 1104 88-89 -0.06504308805592984 1104 90-91 -0.1349092117595987 1104 92-93 -0.11830307401600137 1104 94-95 -0.07379308545949215 1104 96-97 -0.16455478995892392 1104 98-99 -0.1985136719786773 1105 1 0.10659565779468849 1105 2 0.12257098907024044 1105 3 0.09259668805663424 1105 4 -0.0033908141752334586 1105 5 -0.008518756234288105 1105 6 0.1359688687280709 1105 7 0.10367358609752131 1105 8 0.1908455363456909 1105 9 0.0852581700499897 1105 10-11 0.15141058623177628 1105 12-13 0.14367799920054836 1105 14-15 0.21294075270557045 1105 16-17 0.06338551761632516 1105 18-19 0.1852794780727578 1105 20-21 0.1226455709085954 1105 22-23 0.04487185594217635 1105 24-25 -0.020412544240365094 1105 26-27 0.11614872204343385 1105 28-29 0.08731942451719732 1105 30-31 0.26828915182142765 1105 32-33 0.36814716119405233 1105 34-35 0.3655281098571592 1105 36-37 0.4619901908621742 1105 38-39 0.3966573788607235 1105 40-41 0.2513063437170189 1105 42-43 0.07422630727806734 1105 44-45 0.06678585932063896 1105 46-47 0.025891629475566447 1105 48-49 -0.16169765130285896 1105 50-51 -0.03669018642421662 1105 52-53 0.03766868411447177 1105 54-55 -0.048461624181022955 1105 56-57 0.1105184983400207 1105 58-59 -0.06554187756953667 1105 60-61 -0.12594810200495488 1105 62-63 -0.02883672869226217 1105 64-65 0.0755630942621508 1105 66-67 0.1255902383179972 1105 68-69 0.13711241161093568 1105 70-71 0.12899677228327633 1105 72-73 -0.053312483838471536 1105 74-75 0.10202132783899032 1105 76-77 0.138076766197738 1105 78-79 0.06274282172542911 1105 80-81 -0.017392497367463733 1105 82-83 0.07446569765281197 1105 84-85 0.04072515725369996 1105 86-87 -0.03888506956423754 1105 88-89 -0.06352440575367524 1105 90-91 -0.10696992714457565 1105 92-93 -0.09505900014333335 1105 94-95 -0.08746024627381388 1105 96-97 -0.13470063159566692 1105 98-99 -0.15907852621131013 1106 1 0.13251871217953948 1106 2 0.15157867495137367 1106 3 0.029664986077605704 1106 4 -0.002803272607515339 1106 5 0.017290215051126268 1106 6 0.06007946613643611 1106 7 0.0633351507938329 1106 8 -6.891656802565649E-5 1106 9 0.017408862327599195 1106 10-11 0.026943462981009247 1106 12-13 0.00731550617257426 1106 14-15 0.08292324771859683 1106 16-17 -0.03188390880691827 1106 18-19 0.1597038983935306 1106 20-21 0.1099897959465892 1106 22-23 0.06321722361391124 1106 24-25 0.06305193873836146 1106 26-27 0.10028504019065565 1106 28-29 0.06535648482361722 1106 30-31 0.25659625488554383 1106 32-33 0.29503906136882563 1106 34-35 0.36313169299165793 1106 36-37 0.41492987491661637 1106 38-39 0.38468138304689603 1106 40-41 0.226353629188921 1106 42-43 0.145085177504356 1106 44-45 0.04331504309721623 1106 46-47 0.05173359863421467 1106 48-49 -0.023167294517996595 1106 50-51 -0.01517749486648512 1106 52-53 0.05425966273318039 1106 54-55 -0.06259762650785916 1106 56-57 -0.0997600003087058 1106 58-59 -0.06713108592738593 1106 60-61 -0.1482443665623734 1106 62-63 -0.010842194612877165 1106 64-65 0.08256388580294072 1106 66-67 0.06880446994995282 1106 68-69 0.08111886856470107 1106 70-71 0.12582766266002565 1106 72-73 -0.05159424320939365 1106 74-75 0.10217495651490083 1106 76-77 0.042530397438302 1106 78-79 -0.05095156097786102 1106 80-81 -0.024497835320449468 1106 82-83 0.006687398496445951 1106 84-85 0.060404683235361745 1106 86-87 -0.03786759474772339 1106 88-89 -0.089897584973464 1106 90-91 -0.09186021757075835 1106 92-93 -0.1354818625529255 1106 94-95 -0.029834288531908726 1106 96-97 -0.1413800627121624 1106 98-99 -0.10333597703839814 1107 1 0.05529761052920534 1107 2 0.1480328596968299 1107 3 0.027828611713623275 1107 4 0.026516506709370447 1107 5 0.043865353281901776 1107 6 0.09367886589727448 1107 7 0.05538251451655185 1107 8 0.0760028557763448 1107 9 -0.08352516837553026 1107 10-11 0.029899772541227776 1107 12-13 0.031774347799753855 1107 14-15 0.16301012273400772 1107 16-17 0.0014082780592588051 1107 18-19 0.15128915315619906 1107 20-21 0.04331024506354453 1107 22-23 -0.026400412898667014 1107 24-25 0.04911787449681526 1107 26-27 0.07097189677246263 1107 28-29 0.12575151411314067 1107 30-31 0.26465140119024966 1107 32-33 0.29524063409517254 1107 34-35 0.32620445681441623 1107 36-37 0.4834368771753432 1107 38-39 0.38448791947221395 1107 40-41 0.28190409351235246 1107 42-43 0.14928693673702043 1107 44-45 0.2331436728081755 1107 46-47 0.1441338928374165 1107 48-49 -0.033664597613650926 1107 50-51 -0.008719755449561717 1107 52-53 0.12885044021626157 1107 54-55 0.06378624842860603 1107 56-57 0.18546474806427682 1107 58-59 0.11099381343858283 1107 60-61 0.06871829429577758 1107 62-63 0.07336842400362542 1107 64-65 0.19590711800512395 1107 66-67 0.18610408338005868 1107 68-69 0.25287072177922454 1107 70-71 0.20458054257745673 1107 72-73 -0.10053650433001948 1107 74-75 0.15343049191865532 1107 76-77 0.16036493942615593 1107 78-79 0.05553806497564295 1107 80-81 0.03322586495615809 1107 82-83 0.04721129176335026 1107 84-85 0.14741269207227603 1107 86-87 -0.006576957693603447 1107 88-89 -0.034156261352570993 1107 90-91 0.008985764355859516 1107 92-93 0.05518926753327591 1107 94-95 0.05233404670574515 1107 96-97 0.053914281296865596 1107 98-99 -0.05237352716009269 1108 1 0.11440643196821654 1108 2 0.09352154367076793 1108 3 0.04986432641467431 1108 4 -0.014703914276168462 1108 5 0.09451237196466167 1108 6 0.19053041785982572 1108 7 0.12000075465545734 1108 8 0.11629219715585037 1108 9 0.11407771969761171 1108 10-11 0.009916982206306102 1108 12-13 -0.013891802182754986 1108 14-15 0.016967724867889444 1108 16-17 -0.009797441203161839 1108 18-19 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0.1531469193067423 2215 90-91 0.3102663675821553 2215 92-93 0.23365161141519408 2215 94-95 0.38757647548327867 2215 96-97 0.48153153323536557 2215 98-99 0.4932243974884649 2216 1 -0.2906577726487747 2216 2 -0.32611122087348576 2216 3 -0.17816113773148246 2216 4 -0.2123489323648471 2216 5 -0.3143541732786872 2216 6 -0.24527641468979056 2216 7 -0.3651181561627155 2216 8 -0.3164019836283387 2216 9 -0.26911016550000255 2216 10-11 -0.3840342352472703 2216 12-13 -0.36973303237438415 2216 14-15 -0.38407281165599017 2216 16-17 -0.37935957823313515 2216 18-19 -0.3163191937952803 2216 20-21 -0.15458219181824262 2216 22-23 -0.1374968530553886 2216 24-25 -0.27609047702380707 2216 26-27 -0.3509720785170387 2216 28-29 -0.39544055668091715 2216 30-31 -0.15066532699053425 2216 32-33 -0.1132511268657268 2216 34-35 -0.28763001844288993 2216 36-37 -0.12338374658908435 2216 38-39 -8.346799671415244 2216 40-41 -1.919125572580171 2216 42-43 -0.5792682460432275 2216 44-45 -0.5814394873524478 2216 46-47 -0.3222366076904848 2216 48-49 -0.2722022125060306 2216 50-51 -0.2333637939978317 2216 52-53 -0.2728692380505251 2216 54-55 -0.2551991166904841 2216 56-57 -0.19839668026101975 2216 58-59 -0.2528047580696935 2216 60-61 -0.28405355036039737 2216 62-63 -0.3720884766508732 2216 64-65 -0.35567108551897775 2216 66-67 -0.3004152218746441 2216 68-69 -0.2455433105205458 2216 70-71 -0.044694051229623 2216 72-73 -0.13519781628939853 2216 74-75 -0.13679851120503983 2216 76-77 -0.1578028304638721 2216 78-79 -0.18370566465759453 2216 80-81 -0.16634485148860634 2216 82-83 -0.244580802607274 2216 84-85 -0.2109715219221151 2216 86-87 -0.07576616890029086 2216 88-89 -0.022783240269419025 2216 90-91 -0.08794263575373762 2216 92-93 -0.011133892554269664 2216 94-95 0.07792922062774466 2216 96-97 0.1352598094891455 2216 98-99 0.20490850084946644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 8.0 7 212.0 8 1270.0 9 2483.0 10 4088.0 11 9097.0 12 21949.0 13 44241.0 14 63389.0 15 66136.0 16 64461.0 17 64855.0 18 63127.0 19 62358.0 20 62646.0 21 65070.0 22 69080.0 23 74886.0 24 83379.0 25 89402.0 26 96124.0 27 105159.0 28 124928.0 29 154756.0 30 193020.0 31 248766.0 32 327015.0 33 435134.0 34 587152.0 35 824891.0 36 1217104.0 37 1409247.0 38 417539.0 39 400.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.274779289899726 19.042145811151265 12.631422993547764 24.051651905401247 2 15.37295350708364 21.620280084642133 38.04103796490258 24.96572844337165 3 18.09225465131958 28.158835858940684 28.4503049884083 25.29860450133144 4 12.547447171005183 18.41713699733078 35.89173176910728 33.14368406255676 5 13.53787882976939 39.32577371652897 32.307756001238054 14.828591452463582 6 30.313061610815367 37.68723668622611 17.710366049033002 14.289335653925528 7 26.67400315198547 33.797288997597164 21.39747568950391 18.131232160913452 8 26.63640879851509 34.53579366011037 19.33689304916854 19.490904492206 9 26.827041023782666 17.125822939723015 18.753413261061517 37.293722775432805 10-11 24.747470057404463 27.677246264428295 25.50050709791764 22.0747765802496 12-13 25.80681680111073 25.75801741210971 25.310181647998746 23.124984138780814 14-15 24.330175249581057 25.98830922750403 23.177423993567714 26.504091529347196 16-17 23.41119949368837 28.15328459960982 24.426863864310977 24.00865204239084 18-19 24.07844786862227 27.609971514333854 25.84931008884828 22.462270528195592 20-21 26.100041228507443 26.677346664829248 24.46100248221702 22.761609624446294 22-23 25.030675055376015 26.81899764360849 23.91927716249659 24.231050138518903 24-25 24.070054285925536 27.474736476244495 24.02615285905473 24.429056378775243 26-27 24.495526710011546 27.020898888702682 24.075284714820143 24.40828968646563 28-29 23.905816754283748 27.08483589572946 24.903693046121145 24.10565430386565 30-31 24.41882358545646 27.28419209793464 24.597692903385333 23.69929141322357 32-33 23.528254610708252 27.842938683106045 24.188058399256292 24.440748306929414 34-35 23.66279187639499 28.1338313268932 24.79154293741248 23.411833859299332 36-37 24.070010604874838 27.64434964329057 24.368343597944808 23.91729615388978 38-39 24.26546020005747 27.379634165916634 24.34648444640182 24.00842118762408 40-41 23.77111413200671 27.89005707564451 24.715429933181117 23.62339885916766 42-43 24.30415009712402 27.60197171472915 24.55622878934317 23.537649398803662 44-45 23.81548313455487 27.934286461446135 24.443374349415738 23.806856054583257 46-47 23.154163380681066 27.971828839286506 25.195856556759676 23.678151223272753 48-49 23.845600482266335 27.890457659635885 24.90934822022513 23.354593637872654 50-51 22.870781819490034 28.22104759848993 24.664447145299253 24.243723436720778 52-53 22.873820029449817 27.925416759175846 24.18825852211586 25.012504689258474 54-55 23.31358674971347 27.718316856107972 24.194257725241204 24.773838668937355 56-57 23.81937898100234 27.0162531440765 24.02532387833684 25.139043996584316 58-59 23.409701787251926 27.084016074300933 25.00216031470345 24.5041218237437 60-61 23.69331275591078 27.415438723791485 24.527135040505154 24.36411347979258 62-63 23.054846339752935 27.556864843107963 24.400834239578685 24.987454577560413 64-65 22.874620550869896 27.506469651087567 24.65397486033876 24.964934937703777 66-67 24.120751205166336 27.242788266082467 24.258226261982106 24.3782342667691 68-69 24.175434519749018 26.931990675821467 24.313398788974002 24.579176015455513 70-71 23.4661391268386 27.31378952995444 24.3908815495913 24.82918979361566 72-73 24.28825253192186 27.15639580623881 24.479129375879648 24.076222285959684 74-75 24.69126149282633 26.884021052113717 24.55458707489595 23.870130380164007 76-77 24.05940114687095 27.162239075800056 24.89704382964416 23.88131594768483 78-79 24.442191934717137 26.98463017733233 24.77210529382634 23.801072594124197 80-81 23.97384541748259 26.758761624936273 25.216995502293088 24.050397455288053 82-83 24.286843739612642 26.700244586062034 24.76066853665399 24.252243137671336 84-85 24.476848651698386 26.74095444049777 24.346449371868193 24.435747535935654 86-87 24.383493455328882 26.107030793214935 24.698888701744355 24.810587049711827 88-89 24.64783685362383 25.53327157090762 24.990290104526608 24.828601470941948 90-91 24.899807955944777 25.947937070003608 24.639406113098786 24.512848860952833 92-93 24.482105863691864 25.964148778768507 24.78880314266708 24.764942214872544 94-95 23.810597257595376 26.287285003541566 25.196551379964077 24.705566358898977 96-97 24.518627757963525 26.3652157367846 24.45836384868676 24.65779265656512 98-99 24.331715615171127 26.664709118753944 24.176583960267354 24.82699130580757 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1549.0 1 1338.5 2 1081.5 3 1044.5 4 960.5 5 798.0 6 726.0 7 690.0 8 680.0 9 682.0 10 651.5 11 637.0 12 674.0 13 747.0 14 811.0 15 1036.5 16 1323.0 17 1639.0 18 2183.5 19 2972.0 20 4010.5 21 5344.5 22 7629.0 23 10766.5 24 14594.5 25 19631.0 26 25682.0 27 32862.0 28 41833.0 29 52409.5 30 62171.0 31 72138.5 32 82730.0 33 92301.0 34 101012.5 35 110067.5 36 116769.0 37 125468.0 38 138856.0 39 140584.5 40 140788.5 41 151256.5 42 164578.5 43 183966.5 44 209040.0 45 241361.5 46 276613.0 47 307323.0 48 331521.5 49 362167.5 50 396623.0 51 366766.0 52 314930.5 53 301909.0 54 279863.5 55 259988.0 56 237016.5 57 210972.5 58 198126.5 59 173702.0 60 142183.0 61 111250.0 62 88491.0 63 70114.5 64 52007.0 65 40376.5 66 30152.5 67 23024.0 68 18835.0 69 15673.5 70 13635.0 71 12266.0 72 11726.5 73 10512.0 74 7812.5 75 5817.5 76 4257.0 77 2840.0 78 1832.0 79 1208.5 80 800.5 81 536.5 82 336.5 83 233.5 84 162.5 85 122.5 86 99.0 87 66.0 88 46.5 89 28.0 90 19.0 91 17.0 92 12.0 93 13.0 94 9.5 95 6.5 96 5.5 97 4.0 98 4.0 99 6.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.8355232079067998E-5 2 0.0020841095578114977 3 0.006819433315015853 4 1.4177616039533999E-5 5 0.0052315403185880455 6 0.0 7 5.6710464158136E-4 8 0.0 9 0.0 10-11 7.0888080197669995E-6 12-13 7.088808019767E-5 14-15 0.0028709672480056346 16-17 1.2050973633603898E-4 18-19 0.0 20-21 0.0 22-23 3.5444040098835E-5 24-25 9.99521930787147E-4 26-27 0.0024669051908789157 28-29 0.002027399093653362 30-31 0.002523615655037052 32-33 8.931898104906419E-4 34-35 1.4177616039533999E-5 36-37 1.70131392474408E-4 38-39 0.06021942412792066 40-41 0.003913022026911383 42-43 2.6937470475114596E-4 44-45 7.0888080197669995E-6 46-47 8.435681543522729E-4 48-49 1.1342092831627199E-4 50-51 7.017919939569329E-4 52-53 2.55197088711612E-4 54-55 0.0 56-57 4.1115086514648596E-4 58-59 2.1266424059301E-5 60-61 1.48864968415107E-4 62-63 3.5444040098835E-5 64-65 7.7976888217437E-4 66-67 0.001311429483656895 68-69 0.0013681399478150309 70-71 3.5444040098835E-5 72-73 0.0018856229332580219 74-75 0.0016091594204871087 76-77 9.215450425697099E-5 78-79 7.088808019767E-5 80-81 0.0 82-83 3.75706825047651E-4 84-85 5.033053694034569E-4 86-87 0.0 88-89 7.0888080197669995E-6 90-91 6.02548681680195E-4 92-93 0.0 94-95 0.0 96-97 0.0032395852650335187 98-99 0.004685702101065987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7053372.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.17316662006585 #Duplication Level Percentage of deduplicated Percentage of total 1 74.1918094510043 29.80519922919372 2 14.011888213783848 11.258038397481508 3 4.974473035597109 5.995210023182023 4 2.21301410817 3.556151380002793 5 1.2173986620626833 2.44533796470447 6 0.7088293851088467 1.7085552595905908 7 0.4567030747350772 1.284304610206005 8 0.32626223064850823 1.04855895629415 9 0.23421764367993658 0.8468337982421975 >10 1.3775055459647922 10.213111535198646 >50 0.12934533050474262 3.6121119489486633 >100 0.12307685790029592 10.590848748698257 >500 0.022553192557621764 6.242446379398473 >1k 0.01235049184880694 8.645839979946228 >5k 3.221867438534335E-4 0.8994991655724826 >10k+ 2.505896896637816E-4 1.8479526233396497 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 36998 0.5245434382306788 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 34048 0.4827194709140536 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 14837 0.21035328917856597 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 11754 0.1666436989286826 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 10504 0.14892167887926513 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 10278 0.14571753765433043 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 10077 0.1428678368303841 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 9690 0.13738109942308444 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 8059 0.1142574076626045 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 7860 0.11143606207073722 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 7065 0.10016485731930769 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.2532848118602E-5 0.0 5 1.4177616039533999E-5 0.0 0.0 5.6710464158135996E-5 0.0 6 4.2532848118602E-5 0.0 0.0 5.6710464158135996E-5 0.0 7 4.2532848118602E-5 0.0 0.0 5.6710464158135996E-5 0.0 8 4.2532848118602E-5 0.0 0.0 5.6710464158135996E-5 0.0 9 4.2532848118602E-5 0.0 0.0 8.5065696237204E-5 0.0 10-11 4.2532848118602E-5 0.0 0.0 1.0633212029650499E-4 0.0 12-13 5.6710464158136E-5 0.0 0.0 1.27598544355806E-4 0.0 14-15 9.2154504256971E-5 0.0 0.0 1.5595377643487398E-4 1.4177616039533999E-5 16-17 1.27598544355806E-4 0.0 0.0 1.98486624553476E-4 1.4177616039533999E-5 18-19 1.4177616039534E-4 0.0 0.0 2.2684185663254396E-4 1.4177616039533999E-5 20-21 1.70131392474408E-4 0.0 0.0 5.10394177423224E-4 1.4177616039533999E-5 22-23 1.77220200494175E-4 0.0 0.0 0.0028213455918672655 1.4177616039533999E-5 24-25 2.19753048612777E-4 0.0 0.0 0.00808124114253438 1.4177616039533999E-5 26-27 2.9064112881044697E-4 0.0 0.0 0.01334822550122126 1.4177616039533999E-5 28-29 3.3317397692904897E-4 0.0 0.0 0.03148139641578525 1.4177616039533999E-5 30-31 3.8988444108718496E-4 0.0 0.0 0.08532798213393537 1.4177616039533999E-5 32-33 4.04062057126719E-4 0.0 0.0 0.17140737791796606 1.4177616039533999E-5 34-35 4.2532848118601995E-4 0.0 0.0 0.27995120631663833 1.4177616039533999E-5 36-37 4.46594905245321E-4 0.0 0.0 0.4478779794968988 1.4177616039533999E-5 38-39 4.6077252128485493E-4 0.0 0.0 0.6982546787550692 1.4177616039533999E-5 40-41 4.6786132930462195E-4 0.0 0.0 0.9983735438879446 1.4177616039533999E-5 42-43 4.891277533639229E-4 0.0 0.0 1.2639415587324758 2.1266424059301E-5 44-45 5.10394177423224E-4 0.0 0.0 1.5584815319537946 2.8355232079067998E-5 46-47 5.24571793462758E-4 0.0 0.0 1.8745218598990667 2.8355232079067998E-5 48-49 5.24571793462758E-4 0.0 0.0 2.2053848854136717 2.8355232079067998E-5 50-51 5.24571793462758E-4 0.0 0.0 2.580737270060334 2.8355232079067998E-5 52-53 5.24571793462758E-4 0.0 0.0 2.9479871471404033 2.8355232079067998E-5 54-55 5.31660601482525E-4 0.0 0.0 3.29917378524768 2.8355232079067998E-5 56-57 5.52927025541826E-4 0.0 0.0 3.6906390305232732 2.8355232079067998E-5 58-59 5.52927025541826E-4 0.0 0.0 4.108708572297052 2.8355232079067998E-5 60-61 5.52927025541826E-4 0.0 0.0 4.52068882798185 2.8355232079067998E-5 62-63 5.6710464158136E-4 0.0 0.0 4.938510261474937 3.5444040098835E-5 64-65 5.6710464158136E-4 0.0 0.0 5.37713025769802 4.2532848118602E-5 66-67 5.6710464158136E-4 0.0 0.0 5.851846464357757 4.2532848118602E-5 68-69 5.6710464158136E-4 0.0 0.0 6.35891599081971 4.2532848118602E-5 70-71 5.6710464158136E-4 0.0 0.0 6.933775788374695 4.2532848118602E-5 72-73 5.6710464158136E-4 0.0 0.0 7.474715639555095 4.2532848118602E-5 74-75 5.6710464158136E-4 0.0 0.0 8.002066245761602 4.2532848118602E-5 76-77 5.741934496011269E-4 1.4177616039533999E-5 0.0 8.563861370136156 4.2532848118602E-5 78-79 5.812822576208939E-4 1.4177616039533999E-5 0.0 9.133383295252257 4.2532848118602E-5 80-81 5.812822576208939E-4 1.4177616039533999E-5 0.0 9.748897406800605 4.2532848118602E-5 82-83 5.88371065640661E-4 1.4177616039533999E-5 0.0 10.407093231435972 4.2532848118602E-5 84-85 5.95459873660428E-4 2.8355232079067998E-5 0.0 11.040683803434725 4.2532848118602E-5 86-87 6.02548681680195E-4 2.8355232079067998E-5 0.0 11.680214796554045 4.2532848118602E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATA 2340 0.0 34.179756 1 TACATGA 3365 0.0 29.98686 2 GTACTAG 3110 0.0 27.062914 1 GTACAAG 4240 0.0 26.320961 1 GTACAAA 5075 0.0 24.647484 1 TAGGCAT 4075 0.0 22.02161 5 ACGTTAA 705 0.0 20.444069 8 TAGTACT 3745 0.0 20.36158 4 TATTCGC 465 0.0 19.99874 9 TACATAG 2150 0.0 19.89783 2 TACAAGA 3280 0.0 19.847717 2 CTAGGCA 4810 0.0 19.526558 4 CGTTAAC 800 0.0 19.1814 1 GTATAAG 3275 0.0 18.884117 1 CTAGTAC 4200 0.0 18.48943 3 GTGATCG 2425 0.0 18.02233 8 CAACACA 12230 0.0 17.868612 5 GGCGTCG 2450 0.0 17.838428 8 GTCCTAC 2990 0.0 17.729237 1 CTGTGCG 4235 0.0 17.676573 9 >>END_MODULE