FastQCFastQC Report
Fri 27 May 2016
ERR765519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765519_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7053372
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT377210.534793854627262No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA348600.49423169513815524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313040.4438160925015723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211700.30014013155693475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158710.22501294416344408No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG144180.2044128680580012No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC109660.15547173748952983No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT109200.15481956715171125No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG106960.15164378115885566No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG106470.15094907797291848No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC102740.1456608271901723No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA85940.12184243224375518No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC76720.10877067025530485No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC72280.10247580873375174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6000.034.1019862
GTATCAA812750.024.4206771
TCACGCA9550.023.373424
TAGTACT41700.022.7498514
AGTACTT517650.022.5050812-13
GTACTAG28950.021.2138351
CTAACGC12600.020.6681653
TCTAACG13150.020.1570682
ATCACGC11550.020.1313293
CAACGCA984550.020.0268425
ATCAACG985550.019.987653
TACTTTT589900.019.97675514-15
TATCAAC998900.019.8692132
CGTTAAC10100.019.8080121
CTAGTAC47500.019.776173
AACGCAG1013800.019.7423216
GAGTACT500150.019.6661412-13
TCAACGC1021650.019.3633174
CTGTGCG47750.018.9918469
GTATAAG30600.018.8535921
Produced by FastQC (version 0.11.5)