##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765519_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7053372 Sequences flagged as poor quality 0 Sequence length 99 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.291152798973314 33.0 33.0 33.0 33.0 33.0 2 32.123822194547515 33.0 33.0 33.0 27.0 33.0 3 32.16956272829506 33.0 33.0 33.0 27.0 33.0 4 36.11120057186832 37.0 37.0 37.0 33.0 37.0 5 36.05708971538719 37.0 37.0 37.0 33.0 37.0 6 36.065393261549225 37.0 37.0 37.0 33.0 37.0 7 35.98101333659986 37.0 37.0 37.0 33.0 37.0 8 35.94818492488415 37.0 37.0 37.0 33.0 37.0 9 35.67628192586468 37.0 37.0 37.0 33.0 37.0 10-11 35.85465937426808 37.0 37.0 37.0 33.0 37.0 12-13 35.77539976623947 37.0 37.0 37.0 33.0 37.0 14-15 37.0996225635058 37.0 37.0 40.0 33.0 40.0 16-17 37.26256760596208 37.0 37.0 40.0 33.0 40.0 18-19 37.362638323910886 37.0 37.0 40.0 33.0 40.0 20-21 37.39700677916888 37.0 37.0 40.0 33.0 40.0 22-23 37.31231962527994 37.0 37.0 40.0 33.0 40.0 24-25 37.24897417859146 37.0 37.0 40.0 33.0 40.0 26-27 37.08815393545102 37.0 37.0 40.0 33.0 40.0 28-29 37.00115349084098 37.0 37.0 40.0 33.0 40.0 30-31 36.83996533856431 37.0 37.0 40.0 33.0 40.0 32-33 36.603780291185544 37.0 37.0 40.0 33.0 40.0 34-35 36.47633358909752 37.0 37.0 40.0 33.0 40.0 36-37 36.27066061452593 37.0 37.0 40.0 33.0 40.0 38-39 36.08020801965358 37.0 37.0 40.0 33.0 40.0 40-41 35.88401894866739 37.0 37.0 40.0 33.0 40.0 42-43 35.63820800887859 37.0 37.0 40.0 27.0 40.0 44-45 35.32752299183993 37.0 35.0 40.0 27.0 40.0 46-47 34.94171901042508 37.0 33.0 40.0 24.5 40.0 48-49 34.569398083639996 37.0 33.0 40.0 22.0 40.0 50-51 34.20474419894485 37.0 33.0 40.0 22.0 40.0 52-53 34.03517154631856 37.0 33.0 40.0 22.0 40.0 54-55 33.9052831751962 37.0 33.0 40.0 22.0 40.0 56-57 33.739173263511404 37.0 33.0 40.0 22.0 40.0 58-59 33.55218511089448 37.0 33.0 37.0 22.0 40.0 60-61 33.374134527428865 37.0 33.0 37.0 22.0 40.0 62-63 33.0531676905741 37.0 33.0 37.0 22.0 40.0 64-65 32.91645074157438 37.0 33.0 37.0 22.0 40.0 66-67 32.73299728130035 37.0 33.0 37.0 22.0 40.0 68-69 32.49072542891542 37.0 33.0 37.0 22.0 40.0 70-71 32.28896370416873 37.0 33.0 37.0 18.5 37.0 72-73 32.08331582397753 37.0 33.0 37.0 15.0 37.0 74-75 31.882260073621524 33.0 33.0 37.0 15.0 37.0 76-77 31.718858724593005 33.0 33.0 37.0 15.0 37.0 78-79 31.527271495109005 33.0 33.0 37.0 15.0 37.0 80-81 31.393456775000665 33.0 33.0 37.0 15.0 37.0 82-83 31.253924931224383 33.0 33.0 37.0 15.0 37.0 84-85 31.09900300168487 33.0 33.0 37.0 15.0 37.0 86-87 30.998404380202828 33.0 33.0 37.0 15.0 37.0 88-89 30.887534572116714 33.0 33.0 37.0 15.0 37.0 90-91 30.8216594133983 33.0 30.0 37.0 15.0 37.0 92-93 30.710442537271536 33.0 27.0 37.0 15.0 37.0 94-95 30.65028719596811 33.0 27.0 37.0 15.0 37.0 96-97 30.605187277801313 33.0 27.0 37.0 15.0 37.0 98-99 29.787402606866618 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14806537465177882 1101 2 0.3554719257763139 1101 3 0.24470974171555326 1101 4 0.18698081033382863 1101 5 0.07498503417096458 1101 6 0.1802061334056262 1101 7 0.19105934657327595 1101 8 0.17815123871720573 1101 9 0.40508062099572584 1101 10-11 0.33014361279548154 1101 12-13 0.26328716975009314 1101 14-15 0.5031804321206508 1101 16-17 0.3951913241901579 1101 18-19 0.3246450888615229 1101 20-21 0.38609240170418957 1101 22-23 0.20129359378946532 1101 24-25 0.3522359536257369 1101 26-27 0.1759456239459425 1101 28-29 0.19045431681553993 1101 30-31 0.2769833974369078 1101 32-33 0.3598345678633379 1101 34-35 0.39131689033968087 1101 36-37 0.42153508949770213 1101 38-39 0.24211451646507243 1101 40-41 0.17875270461773596 1101 42-43 0.2538463720638049 1101 44-45 0.3519156363135707 1101 46-47 0.39374389988847724 1101 48-49 0.4385701352833067 1101 50-51 0.4132732327749906 1101 52-53 0.3938083399615877 1101 54-55 0.37094105459896554 1101 56-57 0.48315323954219735 1101 58-59 0.4421895147601518 1101 60-61 0.32976335782251454 1101 62-63 0.3318940803250783 1101 64-65 0.280609857905624 1101 66-67 0.30329574339360477 1101 68-69 0.13003823148287807 1101 70-71 0.1551137348628302 1101 72-73 0.2762930804548418 1101 74-75 0.12319735007578814 1101 76-77 0.14220397957396713 1101 78-79 0.4305562170295971 1101 80-81 0.34070236753852257 1101 82-83 0.36811496480495265 1101 84-85 0.3825132093439123 1101 86-87 0.19119943089307156 1101 88-89 0.25862461776518586 1101 90-91 0.16721212006363828 1101 92-93 0.34957025366085404 1101 94-95 0.3331565871243818 1101 96-97 0.3090211514566832 1101 98-99 0.3836314727978092 1102 1 0.12445868509526292 1102 2 0.30200659195634216 1102 3 0.21898467014609935 1102 4 0.1480742125399459 1102 5 0.04483403685535592 1102 6 0.17515857010912583 1102 7 0.18637636936571056 1102 8 0.10123801876758876 1102 9 0.35733117173457174 1102 10-11 0.2882591216422128 1102 12-13 0.30310819695366575 1102 14-15 0.5203639829119027 1102 16-17 0.3367807004925467 1102 18-19 0.2688946128631784 1102 20-21 0.410886987102856 1102 22-23 0.23804749305702444 1102 24-25 0.39121786953960935 1102 26-27 0.2063615578195268 1102 28-29 0.22833173264166362 1102 30-31 0.2568461100698869 1102 32-33 0.35083322640289083 1102 34-35 0.32632507669081434 1102 36-37 0.3261796988373362 1102 38-39 0.19606899134736722 1102 40-41 0.2220182831954105 1102 42-43 0.2066308302581774 1102 44-45 0.27968589506545527 1102 46-47 0.3217387473248863 1102 48-49 0.27824446386980384 1102 50-51 0.2649157409266891 1102 52-53 0.41677833371691264 1102 54-55 0.25882397252994593 1102 56-57 0.38893913265399505 1102 58-59 0.28540152930820284 1102 60-61 0.1853200529832364 1102 62-63 0.313193953672787 1102 64-65 0.2349865459028564 1102 66-67 0.29300338192351205 1102 68-69 0.24199571310786894 1102 70-71 0.2320563907152362 1102 72-73 0.23023357171614833 1102 74-75 0.16505407995779464 1102 76-77 0.18515862743585743 1102 78-79 0.39507514070612615 1102 80-81 0.30835546262116154 1102 82-83 0.34001146870805954 1102 84-85 0.29438051340459026 1102 86-87 0.25466903582940503 1102 88-89 0.28022098381836 1102 90-91 0.18780049674533217 1102 92-93 0.2963705593858812 1102 94-95 0.3496878689367868 1102 96-97 0.23582707319734197 1102 98-99 0.24126142895040203 1103 1 0.09951625107926532 1103 2 0.29863510776931435 1103 3 0.24971924667187295 1103 4 0.21012470552391704 1103 5 0.10512160725885167 1103 6 0.1484693831508892 1103 7 0.15508960939258998 1103 8 0.09703990811311058 1103 9 0.4404169269524729 1103 10-11 0.2639951026959082 1103 12-13 0.2741521527927091 1103 14-15 0.42518887952783047 1103 16-17 0.37454786177527666 1103 18-19 0.2541680575359706 1103 20-21 0.3225003016484891 1103 22-23 0.14610140206662692 1103 24-25 0.2833933172382288 1103 26-27 0.1437028915912677 1103 28-29 0.0728102477462258 1103 30-31 0.09488594840841813 1103 32-33 0.17198073008361092 1103 34-35 0.2862205298405627 1103 36-37 0.25194060925885253 1103 38-39 0.042687210697962996 1103 40-41 -0.01770401834434665 1103 42-43 0.012656781857486976 1103 44-45 0.12895501815996369 1103 46-47 0.2747331769726742 1103 48-49 0.1908778806877791 1103 50-51 0.13807193819489783 1103 52-53 0.2392859517192747 1103 54-55 0.15622085562146282 1103 56-57 0.24893792601457676 1103 58-59 0.2337532493746295 1103 60-61 0.19385283455748947 1103 62-63 0.1856306108563004 1103 64-65 -0.019529819644560575 1103 66-67 0.06755083020603792 1103 68-69 0.09969842665617534 1103 70-71 0.09055032768720395 1103 72-73 0.1657113634983105 1103 74-75 0.1223673723676022 1103 76-77 0.05028289194324387 1103 78-79 0.25491464902357563 1103 80-81 0.06826958667760508 1103 82-83 0.1415531185177663 1103 84-85 0.20798476976120384 1103 86-87 0.17976408160654245 1103 88-89 0.2010585435709693 1103 90-91 -0.007504901830472477 1103 92-93 -0.03265137296505927 1103 94-95 0.1641068634619529 1103 96-97 0.12308841883692523 1103 98-99 0.04432812515501894 1104 1 0.053273838497347015 1104 2 0.28590753563845794 1104 3 0.20150711530684617 1104 4 0.1718107278545702 1104 5 -0.006162697504692005 1104 6 0.13519482819198458 1104 7 0.15789545607684374 1104 8 0.026388156500821935 1104 9 0.4003965179186224 1104 10-11 0.2804553554567022 1104 12-13 0.2118380302161924 1104 14-15 0.4127348031568161 1104 16-17 0.27517818621400636 1104 18-19 0.14155937151262776 1104 20-21 0.26226814180476055 1104 22-23 0.21739148138765785 1104 24-25 0.25021196230547815 1104 26-27 0.109567962856012 1104 28-29 0.050990607137542554 1104 30-31 0.17769679053077425 1104 32-33 0.16772141812077024 1104 34-35 0.1971085293265773 1104 36-37 0.14470445285603972 1104 38-39 -0.021205358717800493 1104 40-41 -0.019349199661348848 1104 42-43 0.08379592200937225 1104 44-45 0.1479151324627921 1104 46-47 0.19993060690087106 1104 48-49 0.28835370686635287 1104 50-51 0.19701702133451704 1104 52-53 0.1733147745470731 1104 54-55 0.16377199041527035 1104 56-57 0.2167198751849142 1104 58-59 0.18603863666562148 1104 60-61 0.14228082909899342 1104 62-63 0.1782384854273218 1104 64-65 0.04245969158684204 1104 66-67 0.11024309981554836 1104 68-69 0.002662932431825027 1104 70-71 0.14542306491645007 1104 72-73 0.1277960587115743 1104 74-75 0.060759016001110666 1104 76-77 0.04916765782308019 1104 78-79 0.2565375536452841 1104 80-81 0.1359929306691008 1104 82-83 0.07856538013930603 1104 84-85 0.13751636847558046 1104 86-87 0.17314677318968563 1104 88-89 0.09040186275093731 1104 90-91 0.07604247928367869 1104 92-93 0.088263256689185 1104 94-95 0.1272825691707986 1104 96-97 0.14522312301475182 1104 98-99 0.11664591369506638 1105 1 0.09223806209688945 1105 2 0.24991545272837357 1105 3 0.21277778719638007 1105 4 0.17903050294793132 1105 5 -0.007819632501927742 1105 6 0.11042645045137078 1105 7 0.10610367914497232 1105 8 0.028852809058491857 1105 9 0.2994921300579563 1105 10-11 0.21979441542914202 1105 12-13 0.24795755247319562 1105 14-15 0.4112399824239077 1105 16-17 0.341291099435189 1105 18-19 0.22120172578445363 1105 20-21 0.32099736057836026 1105 22-23 0.13432067689105764 1105 24-25 0.28813514758154923 1105 26-27 0.13607505476196735 1105 28-29 0.1274382273482857 1105 30-31 0.21292074751887924 1105 32-33 0.22318428374259014 1105 34-35 0.2640807856793188 1105 36-37 0.17748567623526412 1105 38-39 0.09386487612680128 1105 40-41 0.03680620159397563 1105 42-43 0.16137065754479352 1105 44-45 0.1667061183183165 1105 46-47 0.27632166762808197 1105 48-49 0.31516689044529755 1105 50-51 0.2514267975088771 1105 52-53 0.17140945917964956 1105 54-55 0.23170876647107264 1105 56-57 0.2765196669909997 1105 58-59 0.2850582356097249 1105 60-61 0.22562922203949398 1105 62-63 0.21128191100797267 1105 64-65 0.05615222680775389 1105 66-67 0.19309112183586308 1105 68-69 0.10471001096430399 1105 70-71 0.1891335918981767 1105 72-73 0.2515471256056543 1105 74-75 0.21607799360919344 1105 76-77 0.22308812122988897 1105 78-79 0.4066463713409689 1105 80-81 0.31759783369210837 1105 82-83 0.27598297383137904 1105 84-85 0.18828041317524224 1105 86-87 0.3020205648796974 1105 88-89 0.22064190119900928 1105 90-91 0.10506928758491796 1105 92-93 0.20374120117245909 1105 94-95 0.3810307266037931 1105 96-97 0.20649518052567473 1105 98-99 0.15915089849380593 1106 1 0.10117642531802318 1106 2 0.2562480936718501 1106 3 0.1674561075502936 1106 4 0.17850625301029766 1106 5 0.04655447645770039 1106 6 0.11448912173333525 1106 7 0.12821369130771387 1106 8 0.0580549107045627 1106 9 0.3506688764800714 1106 10-11 0.23202837799505716 1106 12-13 0.2585818004652296 1106 14-15 0.4102339235705017 1106 16-17 0.29098899861225647 1106 18-19 0.1516553894404069 1106 20-21 0.28085226534550145 1106 22-23 0.15591895203214534 1106 24-25 0.27651660534315425 1106 26-27 0.05777455399363163 1106 28-29 0.10192963407452993 1106 30-31 0.1476084409524887 1106 32-33 0.1539619694216796 1106 34-35 0.25382031787466275 1106 36-37 0.11967098732532122 1106 38-39 0.07851455546622077 1106 40-41 0.11877606076427583 1106 42-43 0.14437227147710985 1106 44-45 0.1209792548912958 1106 46-47 0.2555375058453251 1106 48-49 0.2785472954722792 1106 50-51 0.126718246834308 1106 52-53 0.15419632120120497 1106 54-55 0.19535980422931232 1106 56-57 0.25809209588141613 1106 58-59 0.2611366015152399 1106 60-61 0.17088209807987198 1106 62-63 0.182985637334788 1106 64-65 0.010353768072469904 1106 66-67 0.19252742411212864 1106 68-69 0.09260776777900759 1106 70-71 0.0638878191804153 1106 72-73 0.1673298480841936 1106 74-75 0.12129038035348216 1106 76-77 0.16978971912798357 1106 78-79 0.2587344908752911 1106 80-81 0.2103812341045348 1106 82-83 0.13109895117285575 1106 84-85 0.16478246650096295 1106 86-87 0.07112145672109449 1106 88-89 0.006666416242094186 1106 90-91 0.022664546122598495 1106 92-93 -0.051605104810658275 1106 94-95 0.19986047210188218 1106 96-97 0.20471034040085812 1106 98-99 0.06959906684100048 1107 1 0.08164019767668407 1107 2 0.24043852830518375 1107 3 0.19450947287995746 1107 4 0.17055524440628034 1107 5 -0.05520790638383488 1107 6 0.09490759469301935 1107 7 0.08617788573457119 1107 8 0.008899745747555698 1107 9 0.34728536111749975 1107 10-11 0.2466020886144804 1107 12-13 0.1734896840914999 1107 14-15 0.2772761142137057 1107 16-17 0.1958387754716071 1107 18-19 0.04668264645687259 1107 20-21 0.16934309537656134 1107 22-23 0.14583527827443277 1107 24-25 0.15648284723273065 1107 26-27 0.0012897843493746564 1107 28-29 0.04613307807423439 1107 30-31 0.06832189705423275 1107 32-33 0.004068900042753398 1107 34-35 0.09115966673223852 1107 36-37 0.032969235646724826 1107 38-39 0.062041012093139614 1107 40-41 0.017776096773410188 1107 42-43 0.06680715482227129 1107 44-45 0.10829733882370363 1107 46-47 0.133977824918027 1107 48-49 0.2306297423149246 1107 50-51 0.08153972972411339 1107 52-53 0.10812423651051262 1107 54-55 0.1498405858904377 1107 56-57 0.22764506518564787 1107 58-59 0.15380869461641566 1107 60-61 0.22949828499499603 1107 62-63 0.24899898989814773 1107 64-65 0.08004286595200938 1107 66-67 0.10057288701297296 1107 68-69 0.05536380725230572 1107 70-71 0.11148025086367142 1107 72-73 0.19171228923249117 1107 74-75 0.16292904199739056 1107 76-77 0.15473616625697062 1107 78-79 0.2484066703015806 1107 80-81 0.10681026002956528 1107 82-83 0.03497500797728392 1107 84-85 0.06598207755549623 1107 86-87 0.10013072188804983 1107 88-89 0.13210376161417514 1107 90-91 0.050767814962952684 1107 92-93 -0.009992527674182128 1107 94-95 0.19805135083126046 1107 96-97 0.09722385221743934 1107 98-99 0.1516562236477199 1108 1 0.12494393275678561 1108 2 0.27755948113023976 1108 3 0.16721428894701518 1108 4 0.09274395832954241 1108 5 -0.02937352893581391 1108 6 0.07868162023167713 1108 7 0.09847638042975149 1108 8 0.005487468448166055 1108 9 0.31597006431011465 1108 10-11 0.19435511285378482 1108 12-13 0.16612741869786163 1108 14-15 0.2385120001305907 1108 16-17 0.22764020951905906 1108 18-19 0.07376298636846457 1108 20-21 0.2508931299751751 1108 22-23 0.07600396747026394 1108 24-25 0.15608287231543017 1108 26-27 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-0.20083963967627483 2215 88-89 -0.061399699733456714 2215 90-91 -0.13232697703940488 2215 92-93 -0.052861715211673044 2215 94-95 -0.055116834251396796 2215 96-97 -0.1076886401839019 2215 98-99 0.05001313109134031 2216 1 -0.24472029033425713 2216 2 0.006370342902002335 2216 3 -0.14744979309234196 2216 4 -0.31844006076207165 2216 5 -0.21889238426809499 2216 6 -0.2375621192625701 2216 7 -0.2898301406149244 2216 8 -0.23093626907970588 2216 9 0.06561597450706813 2216 10-11 -0.2237792222505064 2216 12-13 -0.11887429858172283 2216 14-15 -0.4081788939900406 2216 16-17 -0.587277761756809 2216 18-19 -0.5401439417669991 2216 20-21 -0.8141758402007966 2216 22-23 -0.7684379535392409 2216 24-25 -0.631978589070151 2216 26-27 -0.622904024103228 2216 28-29 -0.7292661106622447 2216 30-31 -0.4727477389617434 2216 32-33 -0.5505686068942666 2216 34-35 -0.5298489410476535 2216 36-37 -0.43313557560303906 2216 38-39 -0.45834352757596264 2216 40-41 -0.4171022175372059 2216 42-43 -0.5417793545739542 2216 44-45 -0.43952410582154755 2216 46-47 -0.7070446809981448 2216 48-49 -0.8100349113876106 2216 50-51 -0.4312575885537058 2216 52-53 -0.4204030078253922 2216 54-55 -0.31245322318550706 2216 56-57 -0.4691504073769295 2216 58-59 -0.4294917109539327 2216 60-61 -0.39985064776626444 2216 62-63 -0.44329055064655165 2216 64-65 -0.42572382732606684 2216 66-67 -0.4302052621480712 2216 68-69 -0.3851276901227223 2216 70-71 -0.3636211094807855 2216 72-73 -0.31620108282826465 2216 74-75 -0.31574744739866745 2216 76-77 -0.2182080274489202 2216 78-79 -0.21096498660799767 2216 80-81 -0.1633608761075358 2216 82-83 -0.16360986383335785 2216 84-85 -0.22669937348227265 2216 86-87 -0.39855412770018717 2216 88-89 -0.1614282405634171 2216 90-91 -0.09235204411072573 2216 92-93 -0.14103748844136987 2216 94-95 -0.24496778736392244 2216 96-97 -0.2963175400533302 2216 98-99 -0.24927805531118707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 35.0 10 126.0 11 296.0 12 495.0 13 973.0 14 2751.0 15 7216.0 16 15535.0 17 28533.0 18 46016.0 19 62458.0 20 78402.0 21 96430.0 22 109796.0 23 94706.0 24 67488.0 25 58487.0 26 66172.0 27 84087.0 28 109574.0 29 142991.0 30 187312.0 31 244656.0 32 318800.0 33 420336.0 34 553255.0 35 772240.0 36 1232709.0 37 1694193.0 38 556699.0 39 599.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01441690974299 18.94836407729324 13.51142176209638 24.52579725086739 2 15.30060587516428 21.6150300030924 37.67854147581384 25.405822645929483 3 17.912283656668045 27.10943078005811 30.133955787387933 24.844329775885917 4 12.824390943792558 17.79494970632486 36.45184175738923 32.928817592493346 5 13.10518169469235 37.76115151732546 33.31034151289203 15.823325275090161 6 30.308184510897767 37.62176445535554 17.18762316803934 14.882427865707351 7 25.35748858843685 33.06255504459427 22.739364944880265 18.84059142208861 8 27.341475821777156 32.03229604223342 20.17564081406737 20.450587321922054 9 27.219075351773895 13.465188552055363 20.249030240723183 39.06670585544756 10-11 25.103581662784833 25.491169330073614 26.927446900574648 22.4778021065669 12-13 26.908023566600487 23.582663724527787 27.26499467205189 22.244318036819834 14-15 24.23475223514128 23.403433297800945 26.804440742730645 25.55737372432713 16-17 22.142173498884986 25.94094723776491 27.77247708815408 24.14440217519602 18-19 22.701595775750945 24.106158019171538 31.49762978615051 21.694616418927005 20-21 23.859885739756812 23.500057844673442 30.825405210444025 21.814651205125717 22-23 23.033032994715153 23.365526446074302 30.376222890271492 23.225217668939056 24-25 22.599056167745015 24.049305778853007 30.420052990257705 22.931585063144265 26-27 23.0595876695572 23.536260387230392 30.398035152548314 23.006116790664098 28-29 22.408119123732593 23.44654443293222 31.246501673242243 22.898834770092943 30-31 23.112356756456347 23.45596545879049 30.83061548433855 22.60106230041461 32-33 22.460732256855305 23.692816712346946 30.413233556942693 23.43321747385506 34-35 22.72882388735487 23.925053151882533 30.785183313739868 22.560939647022728 36-37 23.326516735541524 23.567295188740932 29.990145139090917 23.116042936626624 38-39 23.45482416069931 23.219667132259577 29.989081817887953 23.33642688915316 40-41 23.053611804396535 23.58584659932866 30.12491046835471 23.235631127920094 42-43 23.82351306580739 23.517687710218603 29.349203473175667 23.309595750798344 44-45 23.65585978307972 23.85398610032129 28.654443076059767 23.835711040539227 46-47 23.35006575578319 24.043003828523435 28.62041020947144 23.986520206221932 48-49 24.32861899244787 24.50559817346937 27.350719627434934 23.815063206647828 50-51 23.359167785280572 25.1424425083492 26.71955342777894 24.77883627859129 52-53 23.208184642304698 25.195671025560667 26.17593481911768 25.42020951301696 54-55 23.487772940375184 25.30084901235891 26.050483371641253 25.16089467562465 56-57 23.801236570203773 24.613891697070287 25.877179892899772 25.70769183982617 58-59 23.151061648244273 24.673085440552406 26.90703822228574 25.268814688917583 60-61 23.481768719982444 24.859960597569504 26.54119192919358 25.117078753254475 62-63 22.821176878236397 24.825239615888684 26.520074370102698 25.833509135772225 64-65 22.52566573831637 24.71612868284843 26.785677828987325 25.972527749847874 66-67 23.59499825048218 24.57652169770714 26.471799587488082 25.356680464322594 68-69 23.52963694251848 24.13053019037537 26.578834337279577 25.760998529826573 70-71 22.838340234902645 24.386434951631124 26.696990329768948 26.078234483697283 72-73 23.58496758713421 24.33429003886368 26.661588244601305 25.419154129400802 74-75 23.786920638809352 24.264075395427888 26.600135367877947 25.34886859788481 76-77 23.188689041213195 24.612639174567853 26.7505953180975 25.44807646612145 78-79 23.41394728842895 24.763395378748868 26.45132907472898 25.3713282580932 80-81 22.76966002805069 24.847601531339397 26.65759762891213 25.725140811697784 82-83 22.882636843767777 24.83875088397436 26.15249840785372 26.126113864404143 84-85 22.99136498117496 24.987722184509764 25.682233972630396 26.338678861684876 86-87 22.86278818131243 24.584851047130364 25.984047062879995 26.56831370867721 88-89 22.970779082685556 24.187005874636984 26.200085576090416 26.642129466587043 90-91 23.214876618911582 24.544237844428533 25.79863469131689 26.44225084534299 92-93 22.768365643004852 24.68584235534372 25.83782697078505 26.707965030866376 94-95 22.129965562365577 24.833667218179215 26.366269549276723 26.67009767017848 96-97 23.02906261514907 24.63448964797897 25.71269255192953 26.623755184942432 98-99 22.805354658736277 24.688071180706192 25.670140466148673 26.836433694408857 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 132.0 1 139.5 2 400.5 3 654.0 4 461.5 5 411.0 6 857.0 7 2347.5 8 4168.0 9 4000.0 10 2398.5 11 1547.0 12 1534.0 13 1756.0 14 2196.5 15 2870.5 16 3545.0 17 4327.5 18 5485.0 19 6928.0 20 8701.5 21 10918.0 22 13913.5 23 17805.0 24 22349.0 25 27794.0 26 33937.5 27 40295.5 28 48264.0 29 57690.0 30 65626.5 31 74315.0 32 83826.0 33 91579.5 34 98885.0 35 106368.5 36 111123.5 37 119084.5 38 131577.0 39 132535.0 40 133046.5 41 142521.5 42 155038.0 43 174820.5 44 200028.5 45 231775.0 46 267078.0 47 300712.0 48 326455.5 49 360323.0 50 404385.5 51 376070.5 52 315398.5 53 298055.0 54 278037.5 55 260117.0 56 238497.5 57 213664.0 58 201421.0 59 175953.5 60 142403.0 61 110570.5 62 88187.0 63 69595.5 64 50747.0 65 39186.5 66 29249.0 67 22543.5 68 18152.5 69 15229.5 70 13611.5 71 12335.0 72 12411.5 73 10584.0 74 6997.5 75 5363.5 76 4068.0 77 2533.0 78 1279.0 79 800.0 80 522.0 81 366.0 82 232.5 83 126.0 84 85.0 85 55.5 86 29.0 87 13.0 88 4.0 89 3.5 90 4.0 91 1.5 92 1.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05391747379834779 2 0.008492392007680864 3 0.0 4 0.0 5 0.001970688629495226 6 0.0 7 0.0 8 0.0 9 0.004721146141164821 10-11 0.0 12-13 0.0 14-15 0.0025874149272149547 16-17 0.00434543931611717 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 6.167262977197289E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.19753048612777E-4 54-55 0.0 56-57 0.0010278771628662148 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0017367579648429148 70-71 3.4735159296858296E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 6.238151057394959E-4 80-81 9.85344314747613E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.1190755286974797E-4 92-93 9.357226586092439E-4 94-95 5.95459873660428E-4 96-97 2.1266424059300997E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7053372.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.99355858228865 #Duplication Level Percentage of deduplicated Percentage of total 1 71.18221186010905 25.621011126037168 2 15.526812567046827 11.177304754564313 3 5.521901249493211 5.962586283277403 4 2.556996478497698 3.6814161017405076 5 1.34923069050089 2.4281806949782774 6 0.8027402061566484 1.7336085981994673 7 0.5366955581848927 1.3522308110067431 8 0.36678648687610527 1.0561560722053551 9 0.2732516705668432 0.8851770011030318 >10 1.5463167441158605 10.407397300520792 >50 0.1490023249367488 3.7437723529229214 >100 0.14489209181517534 11.302989327324775 >500 0.027151005356338527 6.800929957553315 >1k 0.015132653726435227 9.841297770757256 >5k 4.392063086745803E-4 1.0045070598883137 >10k+ 4.392063086745803E-4 3.001434787920548 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 37721 0.534793854627262 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 34860 0.49423169513815524 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31304 0.4438160925015723 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21170 0.30014013155693475 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15871 0.22501294416344408 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 14418 0.2044128680580012 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 10966 0.15547173748952983 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 10920 0.15481956715171125 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 10696 0.15164378115885566 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 10647 0.15094907797291848 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 10274 0.1456608271901723 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 8594 0.12184243224375518 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 7672 0.10877067025530485 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 7228 0.10247580873375174 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.8355232079067998E-5 0.0 5 1.4177616039533999E-5 0.0 0.0 5.6710464158135996E-5 0.0 6 1.4177616039533999E-5 0.0 0.0 5.6710464158135996E-5 0.0 7 1.4177616039533999E-5 0.0 0.0 7.088808019767E-5 1.4177616039533999E-5 8 2.8355232079067998E-5 0.0 0.0 7.088808019767E-5 1.4177616039533999E-5 9 2.8355232079067998E-5 0.0 0.0 1.27598544355806E-4 1.4177616039533999E-5 10-11 2.8355232079067998E-5 0.0 0.0 1.4177616039534E-4 1.4177616039533999E-5 12-13 2.8355232079067998E-5 0.0 0.0 1.5595377643487398E-4 1.4177616039533999E-5 14-15 3.5444040098835E-5 0.0 0.0 1.98486624553476E-4 4.2532848118602E-5 16-17 4.9621656138369E-5 0.0 0.0 2.55197088711612E-4 4.2532848118602E-5 18-19 5.6710464158135996E-5 0.0 0.0 2.97729936830214E-4 4.2532848118602E-5 20-21 5.6710464158135996E-5 0.0 0.0 5.812822576208939E-4 4.2532848118602E-5 22-23 6.3799272177903E-5 0.0 0.0 0.002870967248005635 4.2532848118602E-5 24-25 1.0633212029650499E-4 0.0 0.0 0.008187573262830886 4.2532848118602E-5 26-27 1.7013139247440797E-4 0.0 0.0 0.013504179277656134 4.2532848118602E-5 28-29 1.8430900851394198E-4 0.0 0.0 0.031779126352615455 7.088808019767E-5 30-31 1.9139781653370897E-4 0.0 0.0 0.08618572790432717 7.088808019767E-5 32-33 1.98486624553476E-4 0.0 0.0 0.17286058356201828 7.797688821743699E-5 34-35 1.98486624553476E-4 0.0 0.0 0.2823826674674184 8.5065696237204E-5 36-37 1.98486624553476E-4 0.0 0.0 0.4509616109854975 8.5065696237204E-5 38-39 1.98486624553476E-4 0.0 0.0 0.7021039015098027 8.5065696237204E-5 40-41 1.98486624553476E-4 0.0 0.0 1.0014855306086223 8.5065696237204E-5 42-43 2.1266424059300997E-4 0.0 0.0 1.2664864408115721 8.5065696237204E-5 44-45 2.1266424059300997E-4 0.0 0.0 1.5614162984739781 9.9243312276738E-5 46-47 2.2684185663254398E-4 0.0 0.0 1.8768966105856886 9.9243312276738E-5 48-49 2.2684185663254398E-4 0.0 0.0 2.2073768404672265 9.9243312276738E-5 50-51 2.2684185663254398E-4 0.0 0.0 2.582318074248742 9.9243312276738E-5 52-53 2.4810828069184496E-4 0.0 0.0 2.94869602794238 9.9243312276738E-5 54-55 2.6937470475114596E-4 0.0 0.0 3.2992517621358974 9.9243312276738E-5 56-57 2.6937470475114596E-4 0.0 0.0 3.6905964976751546 1.0633212029650499E-4 58-59 2.6937470475114596E-4 0.0 0.0 4.109204788858435 1.20509736336039E-4 60-61 2.6937470475114596E-4 0.0 0.0 4.5217379715687755 1.27598544355806E-4 62-63 2.6937470475114596E-4 0.0 0.0 4.941062232362054 1.27598544355806E-4 64-65 2.6937470475114596E-4 0.0 0.0 5.382113689735917 1.27598544355806E-4 66-67 2.6937470475114596E-4 0.0 0.0 5.859438577746927 1.27598544355806E-4 68-69 2.6937470475114596E-4 0.0 0.0 6.369917820866389 1.27598544355806E-4 70-71 2.6937470475114596E-4 0.0 0.0 6.948130624614723 1.27598544355806E-4 72-73 2.6937470475114596E-4 0.0 0.0 7.4925865245729275 1.27598544355806E-4 74-75 2.6937470475114596E-4 0.0 0.0 8.023467357173278 1.27598544355806E-4 76-77 2.6937470475114596E-4 0.0 0.0 8.589218036422864 1.4177616039534E-4 78-79 2.6937470475114596E-4 0.0 0.0 9.162433230517262 1.4177616039534E-4 80-81 2.6937470475114596E-4 0.0 0.0 9.780945907857973 1.4177616039534E-4 82-83 2.6937470475114596E-4 0.0 0.0 10.44272158054332 1.4177616039534E-4 84-85 2.6937470475114596E-4 0.0 0.0 11.079076787669784 1.4177616039534E-4 86-87 2.6937470475114596E-4 0.0 0.0 11.72206711910275 1.4177616039534E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 600 0.0 34.101986 2 GTATCAA 81275 0.0 24.420677 1 TCACGCA 955 0.0 23.37342 4 TAGTACT 4170 0.0 22.749851 4 AGTACTT 51765 0.0 22.50508 12-13 GTACTAG 2895 0.0 21.213835 1 CTAACGC 1260 0.0 20.668165 3 TCTAACG 1315 0.0 20.157068 2 ATCACGC 1155 0.0 20.131329 3 CAACGCA 98455 0.0 20.026842 5 ATCAACG 98555 0.0 19.98765 3 TACTTTT 58990 0.0 19.976755 14-15 TATCAAC 99890 0.0 19.869213 2 CGTTAAC 1010 0.0 19.808012 1 CTAGTAC 4750 0.0 19.77617 3 AACGCAG 101380 0.0 19.742321 6 GAGTACT 50015 0.0 19.66614 12-13 TCAACGC 102165 0.0 19.363317 4 CTGTGCG 4775 0.0 18.991846 9 GTATAAG 3060 0.0 18.853592 1 >>END_MODULE