FastQCFastQC Report
Fri 27 May 2016
ERR765517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765517_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9972348
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA923250.925810049950122No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT910140.9126636976567605No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG377170.3782158424475359No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT272160.27291466362786376No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC270570.2713202547684858No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC265510.2662462240587673No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG248960.24965033310109117No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG247270.24795564695495984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211070.2116552691502543No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC186530.18704722298098703No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG158080.15851833489966455No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT152010.1524315035937374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139690.1400773418657271No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA132640.13300779314961733No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC123730.12407308689989559No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC119670.12000182905771038No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG108530.10883093931338939No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA108380.10868052338325938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106240.10653458944673812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG53950.025.338541
GTACAAA53250.024.5247861
GTACTAG52450.024.4510331
GTACATA32500.023.2714021
AGGGCTA56850.021.8106465
TAGGCAT75300.021.6436045
GTATAAG59450.021.3349651
GTGATCG41800.021.0115578
TAGTACT63250.020.2001044
CTATTGA59050.020.0434999
GCTATTG59550.019.9540798
TATTCGC9050.019.7209559
CTAGGCA84300.019.6694564
TGATCGC45750.019.1974459
GGGCTAT67050.018.8422786
CATGGGG303400.018.7488124
ATTTAGG65700.018.7333721
GCATCGT64450.018.5827588
CTAGTAC74100.018.1939223
TACATGA48000.018.0070922
Produced by FastQC (version 0.11.5)