##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765517_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9972348 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.471424282425765 33.0 33.0 33.0 27.0 33.0 2 31.372534331934666 33.0 33.0 33.0 27.0 33.0 3 31.386116589593545 33.0 33.0 33.0 27.0 33.0 4 34.99848511102902 37.0 37.0 37.0 33.0 37.0 5 35.179899859090355 37.0 37.0 37.0 33.0 37.0 6 35.176501361565 37.0 37.0 37.0 33.0 37.0 7 34.997609188929225 37.0 37.0 37.0 33.0 37.0 8 34.94833132578205 37.0 37.0 37.0 33.0 37.0 9 34.76585945456376 37.0 37.0 37.0 33.0 37.0 10-11 34.78115530063732 37.0 37.0 37.0 33.0 37.0 12-13 34.72909288765294 37.0 37.0 37.0 33.0 37.0 14-15 36.21092805826672 37.0 37.0 40.0 30.0 40.0 16-17 36.271684562151265 37.0 37.0 40.0 33.0 40.0 18-19 36.26629937102075 37.0 37.0 40.0 30.0 40.0 20-21 36.020803174939346 37.0 37.0 40.0 27.0 40.0 22-23 36.105660271783535 37.0 37.0 40.0 27.0 40.0 24-25 36.04183874249074 37.0 37.0 40.0 27.0 40.0 26-27 36.0283420714961 37.0 37.0 40.0 27.0 40.0 28-29 35.97134055089133 37.0 37.0 40.0 27.0 40.0 30-31 36.04746184148407 37.0 37.0 40.0 27.0 40.0 32-33 35.79621935576256 37.0 37.0 40.0 27.0 40.0 34-35 35.61947487191583 37.0 37.0 40.0 27.0 40.0 36-37 35.70087230208974 37.0 37.0 40.0 27.0 40.0 38-39 35.60415315430228 37.0 37.0 40.0 27.0 40.0 40-41 35.59162456023395 37.0 37.0 40.0 27.0 40.0 42-43 35.475554052064766 37.0 37.0 40.0 27.0 40.0 44-45 35.31905861087078 37.0 33.0 40.0 27.0 40.0 46-47 35.06068936824106 37.0 33.0 40.0 27.0 40.0 48-49 34.79579227479827 37.0 33.0 40.0 24.5 40.0 50-51 34.583504406384534 37.0 33.0 40.0 22.0 40.0 52-53 34.44231930133205 37.0 33.0 40.0 22.0 40.0 54-55 34.32647531955363 37.0 33.0 40.0 22.0 40.0 56-57 34.16274351837701 37.0 33.0 40.0 22.0 40.0 58-59 33.967290150724786 37.0 33.0 40.0 22.0 40.0 60-61 33.72278308979992 37.0 33.0 38.5 22.0 40.0 62-63 33.456822355176534 37.0 33.0 37.0 22.0 40.0 64-65 33.27835094603598 37.0 33.0 37.0 22.0 40.0 66-67 33.03150551906131 37.0 33.0 37.0 22.0 40.0 68-69 32.801722673536865 37.0 33.0 37.0 22.0 40.0 70-71 32.47866445294528 37.0 33.0 37.0 22.0 40.0 72-73 32.23791959526483 37.0 33.0 37.0 22.0 37.0 74-75 32.006069734028536 35.0 33.0 37.0 22.0 37.0 76-77 31.707428731929532 33.0 33.0 37.0 18.5 37.0 78-79 31.58356753093655 33.0 33.0 37.0 18.5 37.0 80-81 31.44738957164351 33.0 33.0 37.0 15.0 37.0 82-83 31.2807849264787 33.0 30.0 37.0 15.0 37.0 84-85 31.102257412196202 33.0 27.0 37.0 15.0 37.0 86-87 30.912421628286538 33.0 27.0 37.0 15.0 37.0 88-89 30.76740713420751 33.0 27.0 37.0 15.0 37.0 90-91 30.6242331795882 33.0 27.0 37.0 15.0 37.0 92-93 30.460304082849895 33.0 27.0 37.0 15.0 37.0 94-95 30.344054579723853 33.0 27.0 37.0 15.0 37.0 96-97 30.22366883907381 33.0 27.0 37.0 15.0 37.0 98-99 30.115663031414467 33.0 27.0 37.0 15.0 37.0 100 29.879311672637176 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 3.0 6 14.0 7 228.0 8 1194.0 9 2217.0 10 4090.0 11 8658.0 12 18474.0 13 34143.0 14 46991.0 15 51290.0 16 56095.0 17 62715.0 18 72481.0 19 83572.0 20 96461.0 21 113037.0 22 127953.0 23 124499.0 24 115207.0 25 114448.0 26 123160.0 27 139952.0 28 166686.0 29 203334.0 30 250755.0 31 314558.0 32 409565.0 33 543032.0 34 726110.0 35 1014990.0 36 1574467.0 37 2336805.0 38 1033797.0 39 1367.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.67169033492136 20.123333750222777 13.449187812773648 23.755788102082217 2 14.357578320559153 22.19158473484294 38.82368561685961 24.627151327738293 3 17.612228313928494 29.026921878505306 29.929866987662113 23.430982819904084 4 12.300206707960122 19.1946673117014 37.362162936255885 31.142963044082588 5 12.243350721192108 39.445177895658205 34.14632580726467 14.165145575885022 6 28.773581121464588 38.315637046277764 18.52472332420106 14.38605850805659 7 24.492223816004685 34.04646322219082 23.40478072552308 18.05653223628141 8 26.432352778359437 34.11398851285705 20.544409785653027 18.90924892313049 9 27.037293600236012 15.237978820076545 20.45406092933267 37.27066665035477 10-11 23.70056854803026 27.32726270408653 27.202091386343163 21.77007736154004 12-13 25.723154325568885 25.45149043109533 26.93691262622905 21.888442617106733 14-15 23.90984189360061 25.000116572866038 25.805657639681744 25.284383893851608 16-17 21.77851250476259 27.74883387773514 27.499497230342108 22.973156387160163 18-19 22.54790630230233 26.98680414397412 29.64714447451733 20.818145079206225 20-21 23.372964541604496 26.201790033084393 29.096621853831177 21.32862357147993 22-23 23.065579883254326 26.3322734677782 27.63053856975917 22.971608079208302 24-25 22.46364786569671 26.98645822848913 27.808191059904264 22.7417028459099 26-27 22.987168705780963 26.057681042776863 27.895281176301605 23.059869075140565 28-29 22.40033487488059 26.084605560794806 28.925133787199268 22.589925777125337 30-31 23.19692325995452 26.284201117018057 28.409197718937456 22.109677904089963 32-33 22.062416874979217 26.814245192836506 28.332643430456912 22.790694501727366 34-35 21.725330451542536 27.20527422291369 28.871005217159603 22.19839010838417 36-37 23.06670055447905 26.3880623008069 28.117288748288242 22.42794839642581 38-39 23.440754950829668 26.101145108145534 27.93935862246123 22.518741318563563 40-41 22.38274892973092 26.482781895041903 28.301993548590325 22.832475626636853 42-43 23.303403416499155 26.322737653846602 27.58886573785169 22.784993191802553 44-45 23.328150774539733 26.523374834935098 27.4783360314541 22.670138359071068 46-47 22.589514047894053 26.656733341071586 28.1974053835187 22.556347227515662 48-49 23.482182293178084 26.66123068951048 27.486599630751513 22.369987386559927 50-51 22.31326658630537 27.259484212918306 26.931557960709245 23.495691240067078 52-53 21.779785449518045 27.721294525293832 25.843856180909135 24.655063844278992 54-55 22.126569919121376 27.7380004359347 25.580825814395936 24.55460383054799 56-57 23.196742707110683 26.397211502127455 25.83564542186411 24.570400368897747 58-59 22.41396620431868 26.400428754773692 27.270610980567422 23.914994060340202 60-61 22.972708401055904 26.88990233005099 26.447293843630792 23.690095425262314 62-63 21.96683921401856 26.725590534201388 26.577544505080635 24.730025746699418 64-65 21.18741395293041 27.014511976829304 26.993438406296434 24.804635663943852 66-67 23.0741346315586 26.19159147309456 26.43457238939576 24.299701505951074 68-69 22.85614482236862 25.80110972439445 26.56700863149622 24.775736821740708 70-71 22.106996278565628 26.355275261865064 26.569924017580345 24.967804441988967 72-73 23.242286402711162 25.899660357530585 26.6805727346081 24.177480505150157 74-75 23.358815943028986 25.867205014190446 26.639416300281333 24.134562742499234 76-77 22.303045232973936 26.109061113640696 27.510388753711148 24.07750489967422 78-79 22.643833618627742 26.225383452335837 27.120821062129718 24.0099618669067 80-81 21.89119570501206 26.4072630680616 27.381932290208375 24.319608936717966 82-83 22.032633036145814 26.049561856832128 26.862787392674647 25.055017714347414 84-85 22.43468287953677 26.49405912096566 26.03780258015095 25.033455419346623 86-87 22.35990120335129 25.707104859671876 26.67360057431934 25.259393362657494 88-89 22.641018305718006 24.931795913552588 27.152784576397405 25.274401204331998 90-91 22.86517288409585 25.391441603062663 26.851163168132942 24.892222344708546 92-93 22.183152561197172 25.509595260870405 27.000012438449733 25.30723973948269 94-95 21.238428747081798 25.744890472949102 27.60382405295929 25.412856727009814 96-97 22.49146469928221 25.34279756841168 26.78006742775971 25.385670304546395 98-99 22.534472354782213 25.518026367126424 26.410952429221226 25.53654884887014 100 22.04328895236257 25.7151272571284 26.768097707022193 25.47348608348684 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1671.0 1 1441.5 2 1427.0 3 1625.5 4 1321.5 5 1111.5 6 1440.0 7 2674.5 8 4388.5 9 4481.0 10 2999.5 11 2052.0 12 1971.0 13 2166.0 14 2507.5 15 3046.0 16 3739.0 17 4550.0 18 5698.0 19 7106.5 20 9053.0 21 11668.5 22 15127.0 23 19381.5 24 24268.0 25 30332.0 26 39075.5 27 48456.0 28 58199.5 29 73583.5 30 92453.5 31 110456.0 32 130731.0 33 150535.5 34 168889.5 35 183384.5 36 197366.0 37 223214.5 38 239047.0 39 245524.0 40 247509.5 41 245769.5 42 253419.5 43 272013.5 44 300886.5 45 341582.5 46 385391.0 47 423440.5 48 470159.0 49 521901.0 50 641479.0 51 647360.5 52 491807.5 53 405926.0 54 367703.5 55 325478.0 56 286058.0 57 239217.0 58 199462.5 59 177954.0 60 147100.0 61 114770.0 62 88149.0 63 67977.5 64 49907.0 65 32106.0 66 22131.0 67 16602.0 68 12781.5 69 9975.5 70 8062.0 71 6476.5 72 5178.5 73 4475.5 74 3573.5 75 2673.5 76 2091.0 77 1589.5 78 1229.5 79 949.5 80 705.5 81 521.0 82 394.0 83 301.5 84 221.0 85 152.5 86 121.0 87 82.0 88 55.5 89 43.0 90 25.5 91 21.0 92 16.5 93 10.0 94 7.5 95 9.0 96 6.5 97 1.5 98 1.0 99 1.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01683655644588416 2 0.005425001213355169 3 0.008694040761513738 4 0.00794196111086376 5 0.019864930505834735 6 0.0038907587260292157 7 0.023615301030409288 8 0.008543624831383743 9 7.420519219746443E-4 10-11 1.5542979446766196E-4 12-13 9.9274513885797E-4 14-15 4.462339260523199E-4 16-17 8.774262590916402E-4 18-19 0.002787708571742583 20-21 0.0057859994456671586 22-23 0.012755270875023613 24-25 0.022035933764044335 26-27 0.02667375827638586 28-29 0.03260515978784535 30-31 0.034946634433535614 32-33 0.035633533847795926 34-35 0.044844002636089314 36-37 0.04174042061107374 38-39 0.046122538042194273 40-41 0.04305906693188004 42-43 0.04965731240024917 44-45 0.0431092055752567 46-47 0.04567630411614196 48-49 0.04818825014931288 50-51 0.048764844548144524 52-53 0.03374832085683331 54-55 0.029732215522362437 56-57 0.021113382725913697 58-59 0.020857675644692705 60-61 0.021429256179186687 62-63 0.02143928390786202 64-65 0.025209709889787237 66-67 0.026423065059502534 68-69 0.027631406364880165 70-71 0.02812777893430915 72-73 0.026067080691528216 74-75 0.0209028004237317 76-77 0.025595777443787562 78-79 0.025370153548592567 80-81 0.024081590413812273 82-83 0.02611721933490488 84-85 0.027450907248724173 86-87 0.02989767304550543 88-89 0.030123296940700425 90-91 0.029892659181167767 92-93 0.032690395481585685 94-95 0.030384017886259086 96-97 0.03317674032233933 98-99 0.033908764515638644 100 0.04273817961426938 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9972348.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.72015439372989 #Duplication Level Percentage of deduplicated Percentage of total 1 70.89719195588248 23.197670668783797 2 14.181975595854205 9.28072862208918 3 5.709112054672778 5.6040908363999336 4 2.8707925988496545 3.757311082669511 5 1.6591078317459793 2.714313220528744 6 0.9720235323983852 1.9082856032648323 7 0.6639330030718765 1.520679325732318 8 0.4825024698889988 1.263004424809924 9 0.3559731783029033 1.0482747618687962 >10 1.8447152493626329 11.102911317521068 >50 0.16869839387035884 3.8719465092281697 >100 0.1498385606551605 10.192934766518528 >500 0.02368465759912028 5.459046505039154 >1k 0.018594653015053714 10.923577448141906 >5k 0.0012481780757310518 2.8547460245369067 >10k+ 6.080867548433329E-4 5.300478882867407 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 92325 0.925810049950122 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 91014 0.9126636976567605 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 37717 0.3782158424475359 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 27216 0.27291466362786376 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 27057 0.2713202547684858 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 26551 0.2662462240587673 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 24896 0.24965033310109117 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 24727 0.24795564695495984 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21107 0.2116552691502543 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 18653 0.18704722298098703 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 15808 0.15851833489966455 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 15201 0.1524315035937374 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13969 0.1400773418657271 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 13264 0.13300779314961733 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 12373 0.12407308689989559 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 11967 0.12000182905771038 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 10853 0.10883093931338939 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 10838 0.10868052338325938 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10624 0.10653458944673812 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.002772867533303E-5 0.0 2.005545735066606E-5 0.0 4 1.002772867533303E-5 1.002772867533303E-5 0.0 6.0166372051998184E-5 0.0 5 1.002772867533303E-5 4.011091470133212E-5 0.0 1.0027728675333031E-4 0.0 6 1.002772867533303E-5 4.011091470133212E-5 0.0 1.0027728675333031E-4 0.0 7 1.002772867533303E-5 4.011091470133212E-5 0.0 1.303604727793294E-4 0.0 8 1.002772867533303E-5 4.011091470133212E-5 0.0 1.303604727793294E-4 0.0 9 1.002772867533303E-5 4.011091470133212E-5 0.0 1.6044365880532848E-4 0.0 10-11 1.5041593012999546E-5 4.011091470133212E-5 0.0 1.65457523142995E-4 0.0 12-13 3.0083186025999092E-5 7.019410072733122E-5 0.0 2.1058230218199366E-4 0.0 14-15 4.512477903899864E-5 8.022182940266424E-5 0.0 2.306377595326597E-4 0.0 16-17 6.0166372051998184E-5 8.022182940266424E-5 0.0 2.6573480989632535E-4 0.0 18-19 6.0166372051998184E-5 9.024955807799728E-5 0.0 3.4094277496132304E-4 0.0 20-21 8.523569374033076E-5 1.1030501542866333E-4 0.0 6.116914491953149E-4 0.0 22-23 9.52634224156638E-5 1.1030501542866333E-4 0.0 0.0020556843784432714 0.0 24-25 1.2033274410399637E-4 1.1030501542866333E-4 0.0 0.005796027174342492 0.0 26-27 1.5041593012999547E-4 1.1531887976632985E-4 0.0 0.009075094451176394 0.0 28-29 1.6044365880532848E-4 1.303604727793294E-4 1.002772867533303E-5 0.02118357682664103 0.0 30-31 1.7047138748066152E-4 1.5041593012999547E-4 1.002772867533303E-5 0.0609134378383105 0.0 32-33 1.905268448313276E-4 1.5041593012999547E-4 1.002772867533303E-5 0.12988916953158874 0.0 34-35 2.0556843784432714E-4 1.604436588053285E-4 1.002772867533303E-5 0.21814822346753243 0.0 36-37 2.2061003085732667E-4 1.955407091689941E-4 1.002772867533303E-5 0.3585915774299092 0.0 38-39 2.2061003085732667E-4 2.2562389519499318E-4 1.002772867533303E-5 0.57420779940692 0.0 40-41 2.2061003085732667E-4 2.306377595326597E-4 1.002772867533303E-5 0.8436027302697419 0.0 42-43 2.306377595326597E-4 2.306377595326597E-4 1.002772867533303E-5 1.0704901192778271 0.0 44-45 2.4066548820799274E-4 2.456793525456593E-4 1.002772867533303E-5 1.3229782995940376 0.0 46-47 2.456793525456593E-4 2.5069321688332577E-4 1.002772867533303E-5 1.5944439564283157 0.0 48-49 2.5069321688332577E-4 2.5069321688332577E-4 1.002772867533303E-5 1.8797228095128649 0.0 50-51 2.5069321688332577E-4 2.5069321688332577E-4 1.002772867533303E-5 2.2011115135572887 0.0 52-53 2.5069321688332577E-4 2.607209455586588E-4 1.002772867533303E-5 2.5201938400063857 0.0 54-55 2.5069321688332577E-4 3.1085958893532393E-4 1.002772867533303E-5 2.820604535662013 0.0 56-57 2.5570708122099226E-4 3.2088731761065696E-4 1.002772867533303E-5 3.164019145741805 5.013864337666515E-6 58-59 2.7074867423399184E-4 3.5097050363665607E-4 1.5041593012999546E-5 3.53564175658531 1.002772867533303E-5 60-61 2.7074867423399184E-4 3.5097050363665607E-4 2.005545735066606E-5 3.8938372387325435 1.002772867533303E-5 62-63 2.7074867423399184E-4 3.5097050363665607E-4 2.005545735066606E-5 4.252062804065803 1.002772867533303E-5 64-65 2.7074867423399184E-4 3.7102596098732214E-4 2.005545735066606E-5 4.641785465168283 1.002772867533303E-5 66-67 2.7074867423399184E-4 3.810536896626552E-4 2.005545735066606E-5 5.050881698071507 1.002772867533303E-5 68-69 2.7074867423399184E-4 3.810536896626552E-4 2.005545735066606E-5 5.499587459242297 1.002772867533303E-5 70-71 2.908041315846579E-4 3.8606755400032167E-4 2.005545735066606E-5 6.026028173104268 1.002772867533303E-5 72-73 2.908041315846579E-4 3.910814183379882E-4 2.005545735066606E-5 6.5114655044128025 1.002772867533303E-5 74-75 2.908041315846579E-4 3.910814183379882E-4 2.005545735066606E-5 6.987135827991562 1.002772867533303E-5 76-77 2.908041315846579E-4 3.9609528267565476E-4 2.005545735066606E-5 7.503067482201784 1.002772867533303E-5 78-79 2.908041315846579E-4 4.0612301135098774E-4 2.005545735066606E-5 8.019104427563098 1.002772867533303E-5 80-81 2.908041315846579E-4 4.111368756886543E-4 2.005545735066606E-5 8.579850001223383 1.002772867533303E-5 82-83 3.0083186025999095E-4 4.111368756886543E-4 2.005545735066606E-5 9.209380779732115 1.002772867533303E-5 84-85 3.0083186025999095E-4 4.111368756886543E-4 2.005545735066606E-5 9.807033408781965 1.002772867533303E-5 86-87 3.0083186025999095E-4 4.4122006171465333E-4 2.005545735066606E-5 10.414849140844263 1.002772867533303E-5 88 3.0083186025999095E-4 4.4122006171465333E-4 2.005545735066606E-5 10.90722064653179 1.002772867533303E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAG 5395 0.0 25.33854 1 GTACAAA 5325 0.0 24.524786 1 GTACTAG 5245 0.0 24.451033 1 GTACATA 3250 0.0 23.271402 1 AGGGCTA 5685 0.0 21.810646 5 TAGGCAT 7530 0.0 21.643604 5 GTATAAG 5945 0.0 21.334965 1 GTGATCG 4180 0.0 21.011557 8 TAGTACT 6325 0.0 20.200104 4 CTATTGA 5905 0.0 20.043499 9 GCTATTG 5955 0.0 19.954079 8 TATTCGC 905 0.0 19.720955 9 CTAGGCA 8430 0.0 19.669456 4 TGATCGC 4575 0.0 19.197445 9 GGGCTAT 6705 0.0 18.842278 6 CATGGGG 30340 0.0 18.748812 4 ATTTAGG 6570 0.0 18.733372 1 GCATCGT 6445 0.0 18.582758 8 CTAGTAC 7410 0.0 18.193922 3 TACATGA 4800 0.0 18.007092 2 >>END_MODULE