FastQCFastQC Report
Fri 27 May 2016
ERR765516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9274218
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT846380.9126160286506096No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA827080.8918056487350201No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG332450.35846688098123203No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG257810.27798570186726257No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC246070.2653269526336344No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC244780.26393599977917276No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG244390.2635154791487541No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT237490.25607549876442415No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA211340.22787905136584025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210180.22662827205485142No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC191570.20656189017769477No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC180190.1942913138336839No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC169640.182915691651846No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA163820.1766402299363677No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC159480.17196059010042677No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT153260.16525382517426268No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG139450.15036308182533556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138290.14911230251434676No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT135010.14557561618672324No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC126420.13631337973724578No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC122900.13251791148321077No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA122520.13210817343305925No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC119680.12904592063719011No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA115170.12418297693670777No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC114330.1232772401942676No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT113950.1228675021441161No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA113740.12264106795850603No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC110210.11883481712420389No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA106620.11496387080829888No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA106250.11456491533841451No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC105490.1137454392381115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104070.11221431284017694No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA100930.10882858263629343No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG97190.10479589761638124No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG94500.10189538352451927No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG93580.10090338613994193No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT93490.10080634291753762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA19200.043.305541
GTACAAA33300.036.5365031
GTACAAG34050.036.421531
TACATGA25100.033.6861572
AGGGCTA65100.028.3543535
TATTAAG18750.025.8040962
CTATTGA73500.025.36819
GTACTAG78450.025.3290481
TATTCGC14600.025.0914949
GGGCACC66650.024.33282794
GCTATTG75800.024.2885538
ACGTTAA16250.024.2778788
TAGGCAT105500.024.1743935
GTGATCG59250.023.8595858
TATTCTC40350.023.746245
GTATAAG69650.023.6732231
TTAAGGT18300.023.6143174
TACATAG18550.023.5500432
TACAAAA42350.023.1817152
AGGTGAT62200.023.0302646
Produced by FastQC (version 0.11.5)