FastQCFastQC Report
Fri 27 May 2016
ERR765515_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765515_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6124885
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA516370.8430688902730419No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT452560.7388873423745915No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG210850.34425136145413343No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG148100.24180045829431898No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC140110.22875531540592192No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC130470.21301624438662928No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT130440.21296726387515846No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG124610.20344871781266097No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC100980.16486840161080574No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA92530.1510722242131893No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC89970.14689255390101202No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT79080.1291126282371016No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG71510.1167532125092961No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC64940.10602648049718486No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG27300.026.9107421
ACGTTAA5850.024.6379818
GTACATA18700.024.1191141
GTATAAG32500.022.7480931
GTGATCG23050.022.591578
CGTTAAC7550.020.3234861
GTACAAG30700.020.29541
ATAAGGT27450.019.818683
TGATCGC26550.019.4387539
TAAGGTG27600.019.3735564
TAGTACT41800.019.1325444
CTAGGCA44350.019.0808774
TAGGCAT41850.018.6652765
CAATGCG17100.018.62504418-19
GTCCTAC26100.018.527811
TATTCGC4550.018.3937849
CGGGCGT20500.018.3710526
GACGTTA7600.018.3531467
TACGGGC21150.018.2468664
GGCGTCG21150.018.2459748
Produced by FastQC (version 0.11.5)