##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765515_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6124885 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89911288130308 33.0 33.0 33.0 27.0 33.0 2 30.856869802453435 33.0 33.0 33.0 27.0 33.0 3 30.871164927994567 33.0 33.0 33.0 27.0 33.0 4 34.2897894409446 37.0 37.0 37.0 27.0 37.0 5 34.48377838930853 37.0 37.0 37.0 27.0 37.0 6 34.45986855263405 37.0 37.0 37.0 27.0 37.0 7 34.3632363709686 37.0 37.0 37.0 27.0 37.0 8 34.27868849782486 37.0 37.0 37.0 27.0 37.0 9 34.16368927743133 37.0 37.0 37.0 27.0 37.0 10-11 34.19952072569526 37.0 37.0 37.0 27.0 37.0 12-13 34.094542101606805 37.0 35.0 37.0 27.0 37.0 14-15 35.38961458051865 37.0 37.0 40.0 27.0 40.0 16-17 35.44472598261029 37.0 37.0 40.0 27.0 40.0 18-19 35.446311808303335 37.0 37.0 40.0 27.0 40.0 20-21 35.45122341072526 37.0 35.0 40.0 27.0 40.0 22-23 35.4822292173649 37.0 33.0 40.0 27.0 40.0 24-25 35.574328824133026 37.0 33.0 40.0 27.0 40.0 26-27 35.526646949289656 37.0 33.0 40.0 27.0 40.0 28-29 35.52538202758093 37.0 33.0 40.0 27.0 40.0 30-31 35.428225264637625 37.0 33.0 40.0 27.0 40.0 32-33 35.30831150299149 37.0 33.0 40.0 27.0 40.0 34-35 35.17775370476343 37.0 33.0 40.0 27.0 40.0 36-37 34.982789880952865 37.0 33.0 40.0 27.0 40.0 38-39 34.67528304613066 37.0 33.0 40.0 27.0 40.0 40-41 34.74589294329608 37.0 33.0 40.0 27.0 40.0 42-43 34.63311964224634 37.0 33.0 40.0 27.0 40.0 44-45 34.51868582022357 37.0 33.0 40.0 24.5 40.0 46-47 34.38896803776724 37.0 33.0 40.0 22.0 40.0 48-49 34.233300951772975 37.0 33.0 40.0 22.0 40.0 50-51 34.03999797547219 37.0 33.0 40.0 22.0 40.0 52-53 33.89232450894996 37.0 33.0 40.0 22.0 40.0 54-55 33.665722866633416 37.0 33.0 37.0 22.0 40.0 56-57 33.423234966860605 37.0 33.0 37.0 22.0 40.0 58-59 33.180897437258004 37.0 33.0 37.0 22.0 40.0 60-61 32.9371403707988 37.0 33.0 37.0 22.0 40.0 62-63 32.66208614529089 37.0 33.0 37.0 22.0 40.0 64-65 32.4592922969166 37.0 33.0 37.0 22.0 40.0 66-67 32.24764685377767 35.0 33.0 37.0 22.0 40.0 68-69 32.03269334852818 33.0 33.0 37.0 22.0 37.0 70-71 31.746253766397245 33.0 27.0 37.0 22.0 37.0 72-73 31.387971365992996 33.0 27.0 37.0 22.0 37.0 74-75 31.138307413118774 33.0 27.0 37.0 22.0 37.0 76-77 30.94201450312945 33.0 27.0 37.0 15.0 37.0 78-79 30.69057116990768 33.0 27.0 37.0 15.0 37.0 80-81 30.50001567376367 33.0 27.0 37.0 15.0 37.0 82-83 30.384366073811997 33.0 27.0 37.0 15.0 37.0 84-85 30.231706146319482 33.0 27.0 37.0 15.0 37.0 86-87 29.934713712992163 33.0 27.0 37.0 15.0 37.0 88-89 29.786344315036118 33.0 27.0 37.0 15.0 37.0 90-91 29.630062197086147 33.0 27.0 37.0 15.0 37.0 92-93 29.343565389390985 33.0 27.0 37.0 15.0 37.0 94-95 29.219263055551245 33.0 27.0 37.0 15.0 37.0 96-97 29.04529448308009 33.0 27.0 37.0 15.0 37.0 98-99 28.01694660389542 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3675707221974136 1101 2 0.44233143507220163 1101 3 0.4596201052746167 1101 4 0.4669269301059913 1101 5 0.3854027129774735 1101 6 0.5465419015711461 1101 7 0.4833236838735644 1101 8 0.6057142030942018 1101 9 0.503790848845874 1101 10-11 0.5175116639588921 1101 12-13 0.6530502696003424 1101 14-15 0.7389337265029639 1101 16-17 0.6618329535218592 1101 18-19 0.6697540027275295 1101 20-21 0.6424938159715339 1101 22-23 0.5788425580779375 1101 24-25 0.5432074652783996 1101 26-27 0.5293490988783205 1101 28-29 0.560022874991084 1101 30-31 0.7692922579963408 1101 32-33 0.8576267917040212 1101 34-35 0.8746602504699723 1101 36-37 0.9652629517318587 1101 38-39 1.0568143068484588 1101 40-41 0.8865001326925821 1101 42-43 0.7322504554051292 1101 44-45 0.6384421771073647 1101 46-47 0.6185984272261962 1101 48-49 0.5354367737681969 1101 50-51 0.5211128852814753 1101 52-53 0.6523558153822151 1101 54-55 0.5058565932277261 1101 56-57 0.6946713630497925 1101 58-59 0.5840216639397013 1101 60-61 0.4284260385848597 1101 62-63 0.533033592031515 1101 64-65 0.6407846701166449 1101 66-67 0.59756906786901 1101 68-69 0.7261046792613328 1101 70-71 0.7302005118478796 1101 72-73 0.5063781981092532 1101 74-75 0.5776387263177618 1101 76-77 0.5874578182705896 1101 78-79 0.6145967463749393 1101 80-81 0.5603400479791496 1101 82-83 0.6495895288989608 1101 84-85 0.7254132323169529 1101 86-87 0.6618792225250232 1101 88-89 0.476987060232954 1101 90-91 0.3670527125965677 1101 92-93 0.4659584321134993 1101 94-95 0.5407137288226593 1101 96-97 0.43008193274555495 1101 98-99 0.4895690985030221 1102 1 0.3631720570302548 1102 2 0.4368333527428163 1102 3 0.3590568574814412 1102 4 0.38599652512701965 1102 5 0.2934925578988583 1102 6 0.4801016334015884 1102 7 0.4750343763061764 1102 8 0.4807419348536115 1102 9 0.4401365881895245 1102 10-11 0.4147805634818411 1102 12-13 0.5715056006919639 1102 14-15 0.7149181908057614 1102 16-17 0.5032200148945449 1102 18-19 0.578978809161697 1102 20-21 0.5794384459910731 1102 22-23 0.5737288574724602 1102 24-25 0.4520224115921536 1102 26-27 0.5840833454866683 1102 28-29 0.47441870422404264 1102 30-31 0.6539798402300931 1102 32-33 0.7577408796079865 1102 34-35 0.8101176273799879 1102 36-37 0.93780375685904 1102 38-39 0.8829385546703179 1102 40-41 0.6190030188445874 1102 42-43 0.5878080903372265 1102 44-45 0.49186702224227474 1102 46-47 0.4594248015217204 1102 48-49 0.36713628369843576 1102 50-51 0.4813179084993422 1102 52-53 0.5506198503509125 1102 54-55 0.5151869488600127 1102 56-57 0.491225370453904 1102 58-59 0.42464919925289735 1102 60-61 0.3483178563391789 1102 62-63 0.4794902071313203 1102 64-65 0.535998233943829 1102 66-67 0.5200323785861585 1102 68-69 0.6084066695725312 1102 70-71 0.6865989640960315 1102 72-73 0.5096519173319294 1102 74-75 0.5242376699770581 1102 76-77 0.5485663196484367 1102 78-79 0.5751339404782669 1102 80-81 0.4633670259228886 1102 82-83 0.3586139914962594 1102 84-85 0.5546474792500078 1102 86-87 0.582510949485112 1102 88-89 0.438171481745929 1102 90-91 0.4664750253658454 1102 92-93 0.5942089319009938 1102 94-95 0.6060506049538041 1102 96-97 0.4478630151072487 1102 98-99 0.43240163423732625 1103 1 0.14517685333827401 1103 2 0.2229701609867938 1103 3 0.11280548755798137 1103 4 0.1826130634215275 1103 5 0.10851587375695715 1103 6 0.3442319831443825 1103 7 0.23213743559104216 1103 8 0.21509977169204575 1103 9 0.15542107134356797 1103 10-11 0.20529950618033865 1103 12-13 0.2664878114696805 1103 14-15 0.3913731172503603 1103 16-17 0.2160672368994483 1103 18-19 0.19577656987722492 1103 20-21 0.28085173042845213 1103 22-23 0.2016232664501274 1103 24-25 0.12317563254072184 1103 26-27 0.29203326715614963 1103 28-29 0.23241923828965838 1103 30-31 0.4808534019613475 1103 32-33 0.5475806500876388 1103 34-35 0.6060816497944685 1103 36-37 0.7019077207382196 1103 38-39 0.7046704942351596 1103 40-41 0.45485548610165694 1103 42-43 0.3303360670869111 1103 44-45 0.28364337294411257 1103 46-47 0.26900041114827644 1103 48-49 0.18822711875513676 1103 50-51 0.12944636750857796 1103 52-53 0.2359629613560017 1103 54-55 0.1178525246248654 1103 56-57 0.21022094127688007 1103 58-59 0.1649496784455735 1103 60-61 0.19957274735823205 1103 62-63 0.21264818459899004 1103 64-65 0.32338248060864316 1103 66-67 0.28083301295560403 1103 68-69 0.3899112879033808 1103 70-71 0.3623601774845291 1103 72-73 0.046918149622843686 1103 74-75 0.15601228069615658 1103 76-77 0.14121920453713344 1103 78-79 0.17379477295718004 1103 80-81 0.11198543626356994 1103 82-83 0.30423366319030976 1103 84-85 0.29626159623246195 1103 86-87 0.18425437239953624 1103 88-89 0.12367731377286262 1103 90-91 0.0600046970339676 1103 92-93 0.1505061471507183 1103 94-95 0.2957676802735101 1103 96-97 0.031350131858392416 1103 98-99 0.015651995757636428 1104 1 0.21423184922386795 1104 2 0.14169382255824914 1104 3 0.1110980093144569 1104 4 0.13016623899442692 1104 5 0.11362116513166853 1104 6 0.3043172635473397 1104 7 0.13749114977701282 1104 8 0.2762862789358991 1104 9 0.22315602882804342 1104 10-11 0.1549596001882776 1104 12-13 0.2352058624883142 1104 14-15 0.24488236219463033 1104 16-17 0.16200426156094494 1104 18-19 0.312706568879797 1104 20-21 0.24594520847247736 1104 22-23 0.186816082448928 1104 24-25 0.1254648939426133 1104 26-27 0.27022878868820754 1104 28-29 0.21022950186095812 1104 30-31 0.4179198309068113 1104 32-33 0.48857010224832464 1104 34-35 0.5439566217417067 1104 36-37 0.5434480980167251 1104 38-39 0.557575981355825 1104 40-41 0.34607200980313024 1104 42-43 0.3038960251947813 1104 44-45 0.2507164410437497 1104 46-47 0.19849836017952072 1104 48-49 0.037918850047056196 1104 50-51 0.03591891943735703 1104 52-53 0.13502771336956698 1104 54-55 -0.01894163665982518 1104 56-57 0.18663137191797574 1104 58-59 0.1859730432811233 1104 60-61 0.02911854193719421 1104 62-63 0.16047534216824744 1104 64-65 0.19504373413082732 1104 66-67 0.13359932719644263 1104 68-69 0.22734723409195112 1104 70-71 0.27934113900860424 1104 72-73 0.10349901639091286 1104 74-75 0.1108216154092716 1104 76-77 0.16579467270659975 1104 78-79 0.16725894646041084 1104 80-81 0.038805904807070135 1104 82-83 0.222577833202525 1104 84-85 0.2586685545647569 1104 86-87 0.20603594111010892 1104 88-89 0.15495661332651167 1104 90-91 0.03587183914303438 1104 92-93 0.014714092940309342 1104 94-95 0.10351710051638463 1104 96-97 0.07008897309221496 1104 98-99 -0.005525191734570001 1105 1 0.14138954575755847 1105 2 0.11840334493549776 1105 3 0.14599482413973064 1105 4 0.05480714722039437 1105 5 0.024153372901551506 1105 6 0.2839972728775919 1105 7 0.1311453536060938 1105 8 0.22408622798783284 1105 9 0.140208130896049 1105 10-11 0.20288243075219725 1105 12-13 0.22066111105961284 1105 14-15 0.2771697384307288 1105 16-17 0.16878933132296225 1105 18-19 0.27475014635462003 1105 20-21 0.28686053186991955 1105 22-23 0.18250736480555219 1105 24-25 0.10158939597133099 1105 26-27 0.2768879461422742 1105 28-29 0.20322107394633093 1105 30-31 0.3991620429121383 1105 32-33 0.45806111267733485 1105 34-35 0.4969297174810947 1105 36-37 0.5422695787638787 1105 38-39 0.4571650591344891 1105 40-41 0.40744520590406097 1105 42-43 0.2143503530179558 1105 44-45 0.14877561906119752 1105 46-47 0.10877509870547186 1105 48-49 0.053938716722733204 1105 50-51 0.05837861368759434 1105 52-53 0.08002732205160612 1105 54-55 0.06725608019170437 1105 56-57 0.21448607479255344 1105 58-59 0.07874225469103635 1105 60-61 0.02469097105492324 1105 62-63 0.08229239258227494 1105 64-65 0.1593551909999178 1105 66-67 0.12932375648605188 1105 68-69 0.18502324277153548 1105 70-71 0.14169888342119563 1105 72-73 -0.023348234562057257 1105 74-75 0.05447815947320933 1105 76-77 0.04185598839430682 1105 78-79 0.0686463988116941 1105 80-81 0.004720384185379345 1105 82-83 0.08810759331694484 1105 84-85 0.13106801654092948 1105 86-87 -0.015434562723271483 1105 88-89 0.02167252723042168 1105 90-91 -0.01958190553964556 1105 92-93 0.016018447772090383 1105 94-95 -0.024410725106196907 1105 96-97 -0.12037541390436601 1105 98-99 -0.06822695067043227 1106 1 0.10662804659575187 1106 2 0.11819639008198024 1106 3 0.12338035123884694 1106 4 -0.03077389088126381 1106 5 0.005622135221088342 1106 6 0.18019508736441736 1106 7 0.14669873667192945 1106 8 0.13910515375457777 1106 9 0.11322913745969743 1106 10-11 0.12193237801025703 1106 12-13 0.07810034001712296 1106 14-15 0.27071883480260794 1106 16-17 0.038878875208304464 1106 18-19 0.19586622203457438 1106 20-21 0.08959299715824187 1106 22-23 0.005995775447757978 1106 24-25 0.11315971513401735 1106 26-27 0.20267414847814536 1106 28-29 0.13334302986502422 1106 30-31 0.3398631311693805 1106 32-33 0.3116052886462697 1106 34-35 0.3768845780662531 1106 36-37 0.4093966205429993 1106 38-39 0.4123709237318991 1106 40-41 0.2887917105749125 1106 42-43 0.13133899801392346 1106 44-45 0.14401941955489406 1106 46-47 0.20149106308734588 1106 48-49 0.05180038590976466 1106 50-51 -0.006006348484810076 1106 52-53 0.04746723579179246 1106 54-55 -0.11349272548574874 1106 56-57 -0.05910428942595303 1106 58-59 -0.03711860415717183 1106 60-61 -0.14391909263594016 1106 62-63 -0.032376062778290304 1106 64-65 0.12798465813335014 1106 66-67 0.06306502826200955 1106 68-69 0.005648942612211272 1106 70-71 0.14855541248201476 1106 72-73 -0.062190802872549966 1106 74-75 0.06690536901267663 1106 76-77 0.033733523410756305 1106 78-79 -0.020501057395463818 1106 80-81 0.026512813863480034 1106 82-83 0.03340607382142835 1106 84-85 0.04084121059002399 1106 86-87 -0.06889294705112547 1106 88-89 -0.1153459122722218 1106 90-91 -0.1894240840332273 1106 92-93 -0.20594747285133863 1106 94-95 -0.0524282929359714 1106 96-97 -0.18531701851054194 1106 98-99 -0.13925878896078814 1107 1 0.037149246566777094 1107 2 0.07760995027811646 1107 3 0.008368319775868827 1107 4 -0.06668074687838299 1107 5 -0.029321554527150795 1107 6 -0.00510302376624594 1107 7 0.026441395985678184 1107 8 0.03611761547718828 1107 9 -0.11608321444856529 1107 10-11 0.021445322189933336 1107 12-13 0.01726910514764768 1107 14-15 0.13984078379364462 1107 16-17 -0.08805641379434093 1107 18-19 0.15974391052763792 1107 20-21 0.03657568854431048 1107 22-23 -0.06354428707450666 1107 24-25 0.019246809880939963 1107 26-27 0.022944361561762605 1107 28-29 0.12494292479274094 1107 30-31 0.29499690965150194 1107 32-33 0.31002760845615285 1107 34-35 0.2915697654967033 1107 36-37 0.42436271762082356 1107 38-39 0.3539069636652741 1107 40-41 0.18930679537010775 1107 42-43 0.10762738418245732 1107 44-45 0.15329873857044163 1107 46-47 0.006575723011131629 1107 48-49 -0.09983524992347981 1107 50-51 -0.05666544736445189 1107 52-53 0.053789638005859786 1107 54-55 -0.0332990741256296 1107 56-57 0.09865508791522615 1107 58-59 0.07115133413001473 1107 60-61 0.021983663778911477 1107 62-63 0.028845507429679174 1107 64-65 0.11865118211837 1107 66-67 0.08259292296529708 1107 68-69 0.1340828122965192 1107 70-71 0.1448939335821926 1107 72-73 -0.2180843122660896 1107 74-75 0.014757709880449 1107 76-77 0.04212032400217325 1107 78-79 -0.009686522879242432 1107 80-81 -0.03837060436087114 1107 82-83 -0.033705310271891165 1107 84-85 -0.00596718286877973 1107 86-87 -0.14078965056228654 1107 88-89 -0.24792257257155725 1107 90-91 -0.12925447432058235 1107 92-93 -0.19327313366986587 1107 94-95 0.013393066762645844 1107 96-97 -0.11796683771290617 1107 98-99 -0.1506492430245352 1108 1 0.0928760177220802 1108 2 0.07403360200176934 1108 3 0.02526885955802527 1108 4 0.006918085359991721 1108 5 -0.12762782638613146 1108 6 0.10038709562903136 1108 7 0.0908214405089609 1108 8 0.08899691046256208 1108 9 0.02554509140606598 1108 10-11 0.034068453413709676 1108 12-13 -0.04021555386307085 1108 14-15 0.0634089740798629 1108 16-17 -0.07113521908276965 1108 18-19 0.18247477455673078 1108 20-21 0.09934886874822269 1108 22-23 -0.00586856724750362 1108 24-25 -0.018416175893847253 1108 26-27 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2216 10-11 -0.4337104981273967 2216 12-13 -0.49124894658381635 2216 14-15 -0.48004936953238087 2216 16-17 -0.4774752578508341 2216 18-19 -0.38483586367172506 2216 20-21 -0.20747713146258207 2216 22-23 -0.22811854460565684 2216 24-25 -0.3444798566572942 2216 26-27 -0.4488852219327484 2216 28-29 -0.46161983307495547 2216 30-31 -0.25017661508124434 2216 32-33 -0.30410296454899566 2216 34-35 -0.3455902568335958 2216 36-37 -0.23485662122900663 2216 38-39 -8.601309861927739 2216 40-41 -2.0698302127621844 2216 42-43 -0.7083187696208029 2216 44-45 -0.8128121020163519 2216 46-47 -0.5663670948090314 2216 48-49 -0.3226510308053747 2216 50-51 -0.4001551662856073 2216 52-53 -0.3085485776084127 2216 54-55 -0.28118984523569424 2216 56-57 -0.27465824717897647 2216 58-59 -0.3923488746144912 2216 60-61 -0.4578743238395475 2216 62-63 -0.4832551731171293 2216 64-65 -0.5072848992660468 2216 66-67 -0.3986668604811854 2216 68-69 -0.4384784637851382 2216 70-71 -0.20611741580718856 2216 72-73 -0.16913913847625395 2216 74-75 -0.27455448689925177 2216 76-77 -0.33192838554190374 2216 78-79 -0.38973456685745944 2216 80-81 -0.34291339770287976 2216 82-83 -0.4300765272054683 2216 84-85 -0.35781992132969975 2216 86-87 -0.264076723603516 2216 88-89 -0.23317444155520306 2216 90-91 -0.260614617571445 2216 92-93 -0.1531538263197092 2216 94-95 -0.055970982600253905 2216 96-97 0.07279222338717162 2216 98-99 0.03387508629343117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 191.0 8 1151.0 9 2112.0 10 3618.0 11 7373.0 12 15243.0 13 27078.0 14 36617.0 15 39779.0 16 42279.0 17 43687.0 18 45978.0 19 49334.0 20 53746.0 21 59507.0 22 66385.0 23 75035.0 24 84462.0 25 93476.0 26 103292.0 27 116121.0 28 136211.0 29 165539.0 30 202624.0 31 251608.0 32 321869.0 33 415723.0 34 544882.0 35 742145.0 36 1029788.0 37 1061894.0 38 285870.0 39 264.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47727589987404 18.756858292033236 12.174285721282931 24.591580086809795 2 15.391485337586225 21.352846210212423 37.69391729591417 25.561751156287183 3 18.411584235851567 27.629238488504427 28.235978924097033 25.723198351546976 4 12.779099163347421 17.99697757541204 36.16426041701361 33.05966284422693 5 13.902535676446254 38.5058254806591 33.043881200262746 14.547757642631904 6 31.246905043931438 36.57093969927598 18.014950484784613 14.167204772007963 7 27.100678335754893 32.39247112130058 22.233027034325616 18.273823508618914 8 27.176951077448802 33.47762447784734 20.123953347695508 19.22147109700835 9 27.304904500247762 15.812884650079145 19.072162171208113 37.81004867846499 10-11 25.2333248080025 26.526900221547034 26.10936581742888 22.13040915302158 12-13 26.362177930372177 24.288936973285512 25.756786378963568 23.592098717378743 14-15 24.749090816470808 24.74715603604145 23.348097531220173 27.15565561626757 16-17 24.047662352674568 27.04841698019682 24.970415727600038 23.93350493952857 18-19 24.728668374998062 26.654378000566542 25.955956724085432 22.660996900349968 20-21 25.78797805999623 26.024178413145716 24.73645627632192 23.451387250536133 22-23 24.84415615585021 26.12348713387248 23.987649719235364 25.04470699104195 24-25 24.350410011714622 26.7034323429649 24.06272832285004 24.883429322470437 26-27 24.96204741811418 25.98422824906429 23.926519099234778 25.127205233586754 28-29 24.438827357266337 26.015696278256602 25.061292157542724 24.48418420693434 30-31 25.231791793648423 26.12745554641865 24.62147932525695 24.01927333467598 32-33 23.96591584433226 26.575143187413875 24.3827675498462 25.076173418407667 34-35 23.99665463106654 26.756984008679346 25.193387304414692 24.052974055839414 36-37 24.81439838938872 26.329415950142366 24.50865359881774 24.347532061651172 38-39 25.202606544001256 25.85833482199123 24.382762411588217 24.556296222419295 40-41 24.37519108102162 26.50686544671426 24.704464754761158 24.413478717502958 42-43 25.426664610566423 26.04926624079051 24.394870187719285 24.12919896092378 44-45 24.989246735999675 26.331965933398415 24.343544763986976 24.33524256661493 46-47 24.214096939459413 26.333975146750937 25.48944599795619 23.96248191583346 48-49 25.004163346873735 26.442428259818968 25.00121635036115 23.552192042946146 50-51 23.728553987847004 26.88965525967874 24.873410720546822 24.50838003192743 52-53 23.77713203101326 26.73508342389317 24.035774508625824 25.452010036467748 54-55 24.225671175866974 26.56564164061856 24.0470637407886 25.161623442725862 56-57 24.922371574620474 25.53433753199364 23.98416091951959 25.559129973866302 58-59 24.386661953653007 25.656040888930974 25.20452220735573 24.752774950060285 60-61 24.66009711709195 26.070411728436234 24.58859361118338 24.680897543288438 62-63 23.530745283685032 26.146093414853265 24.77329156926684 25.549869732194864 64-65 23.341236937929423 26.08134005078498 25.03061915469663 25.54680385658897 66-67 24.886847279440666 25.718964586176114 24.459543356331373 24.934644778051844 68-69 24.90592552264755 25.1259734703239 24.529203109684452 25.438897897344106 70-71 23.795401479505813 25.63327332130263 24.65131987011017 25.920005329081384 72-73 24.910468132336955 25.365015479345406 24.748764219809235 24.975752168508407 74-75 25.32807099422926 25.0008592146052 24.763258824256447 24.907810966909093 76-77 24.340021709814863 25.42661451543659 25.475113428762146 24.758250345986397 78-79 24.7028328806849 25.453811042945585 25.13731496877856 24.706041107590952 80-81 23.818561491358615 25.53983462546644 25.538732563958344 25.102871319216607 82-83 24.081984447340833 25.734419789223246 24.684683051996704 25.498912711439214 84-85 24.290754638273068 25.987096837394517 24.136399963754243 25.58574856057817 86-87 24.18587451029693 25.096536506399715 24.748938143328406 25.968650839974956 88-89 24.492059850919652 24.587939202123795 25.24120861044738 25.678792336509176 90-91 24.83748584251274 25.068944534324185 24.78812137634042 25.30544824682266 92-93 24.066108996332176 25.32848371846982 24.951268472795814 25.654138812402195 94-95 23.256583592998073 25.561614626233798 25.67764129448961 25.504160486278515 96-97 24.537605395078575 25.178576297620143 24.84434730034632 25.43947100695496 98-99 24.616386378379474 25.075682654193663 24.50368490152887 25.804246065897996 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 560.0 1 516.0 2 504.5 3 517.0 4 445.5 5 399.0 6 392.0 7 386.0 8 383.5 9 380.5 10 372.0 11 376.5 12 390.0 13 414.5 14 457.0 15 594.5 16 722.0 17 837.0 18 1207.5 19 1892.0 20 2841.0 21 3911.5 22 4988.5 23 6437.0 24 8369.5 25 11418.5 26 15774.5 27 19864.0 28 24943.5 29 31687.0 30 38117.5 31 45966.0 32 54879.5 33 62816.0 34 69345.0 35 77578.5 36 84884.0 37 93932.0 38 107932.0 39 111562.0 40 115021.5 41 124289.5 42 135391.0 43 152234.5 44 171501.5 45 195855.5 46 226493.5 47 260282.0 48 288271.0 49 329356.0 50 386794.0 51 358307.5 52 289065.5 53 270393.5 54 250857.0 55 236671.0 56 219250.5 57 200501.5 58 194273.5 59 174239.5 60 143134.5 61 111005.5 62 89767.0 63 72380.0 64 52334.5 65 38259.0 66 27651.5 67 20839.5 68 16425.5 69 14022.0 70 12828.5 71 11718.5 72 11444.5 73 10261.5 74 7429.5 75 5402.0 76 4009.5 77 2722.0 78 1673.0 79 1052.5 80 755.5 81 535.5 82 405.0 83 306.5 84 197.5 85 153.0 86 115.5 87 77.5 88 51.5 89 42.5 90 38.5 91 29.0 92 17.5 93 7.5 94 6.0 95 5.5 96 5.0 97 3.5 98 3.5 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0017796252501067367 3 0.005796027190714601 4 1.632683715694254E-5 5 0.005322548913163268 6 0.0 7 6.857271605915866E-4 8 0.0 9 0.0 10-11 8.16341857847127E-6 12-13 9.796102294165523E-5 14-15 0.0025959671079538635 16-17 1.4694153441248284E-4 18-19 0.0 20-21 0.0 22-23 6.530734862777016E-5 24-25 9.796102294165523E-4 26-27 0.002236776690501128 28-29 0.001975547295990047 30-31 0.00250616950359068 32-33 7.347076720624142E-4 34-35 0.0 36-37 8.979760436318396E-5 38-39 0.05876845034641467 40-41 0.0031429161527114385 42-43 3.510269988742646E-4 44-45 8.16341857847127E-6 46-47 8.489955321610119E-4 48-49 8.163418578471269E-5 50-51 9.387931365241959E-4 52-53 3.428635802957933E-4 54-55 0.0 56-57 5.632758819145176E-4 58-59 0.0 60-61 1.7143179014789665E-4 62-63 4.081709289235634E-5 64-65 6.938905791700579E-4 66-67 0.0014285982512324722 68-69 0.001363290902604702 70-71 3.265367431388508E-5 72-73 0.0018041155058421505 74-75 0.0017224813200574377 76-77 8.979760436318396E-5 78-79 1.0612444152012649E-4 80-81 0.0 82-83 2.612293945110806E-4 84-85 4.898051147082761E-4 86-87 0.0 88-89 0.0 90-91 5.551124633360464E-4 92-93 0.0 94-95 0.0 96-97 0.0031347527341329673 98-99 0.004865397472768877 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6124885.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.053784314347986 #Duplication Level Percentage of deduplicated Percentage of total 1 75.04743705682205 27.80791546048091 2 13.730177601100266 10.175100788577222 3 4.739991184498653 5.2690383300697174 4 2.1194819503077515 3.141393081794282 5 1.0881974680003221 2.0160917135351766 6 0.6693986720667555 1.488225240904351 7 0.43959474288465294 1.1402054151998435 8 0.2871391987669206 0.8511675151443336 9 0.22380743129760916 0.7463621058524877 >10 1.2803376685764796 9.128716409743278 >50 0.1665108879374832 4.345111319387367 >100 0.16559374888934864 12.979461366065415 >500 0.02702829305462872 6.885564543819774 >1k 0.014543365889308057 9.849506588034638 >5k 3.579905449675156E-4 0.9353747365726534 >10k+ 4.02739363088455E-4 3.2407653848186295 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 51637 0.8430688902730419 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 45256 0.7388873423745915 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 21085 0.34425136145413343 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 14810 0.24180045829431898 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 14011 0.22875531540592192 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 13047 0.21301624438662928 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 13044 0.21296726387515846 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 12461 0.20344871781266097 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 10098 0.16486840161080574 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 9253 0.1510722242131893 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 8997 0.14689255390101202 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 7908 0.1291126282371016 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 7151 0.1167532125092961 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 6494 0.10602648049718486 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.632683715694254E-5 2 0.0 0.0 0.0 0.0 1.632683715694254E-5 3 0.0 0.0 0.0 0.0 1.632683715694254E-5 4 0.0 0.0 0.0 1.632683715694254E-5 1.632683715694254E-5 5 1.632683715694254E-5 0.0 0.0 1.632683715694254E-5 1.632683715694254E-5 6 1.632683715694254E-5 0.0 0.0 4.8980511470827614E-5 3.265367431388508E-5 7 1.632683715694254E-5 0.0 0.0 4.8980511470827614E-5 3.265367431388508E-5 8 3.265367431388508E-5 0.0 0.0 6.530734862777016E-5 4.8980511470827614E-5 9 3.265367431388508E-5 0.0 0.0 1.632683715694254E-4 6.530734862777016E-5 10-11 3.265367431388508E-5 0.0 0.0 1.9592204588331046E-4 6.530734862777016E-5 12-13 3.265367431388508E-5 0.0 0.0 2.2041230161872426E-4 8.979760436318396E-5 14-15 3.265367431388508E-5 0.0 0.0 2.3673913877566682E-4 1.1428786009859777E-4 16-17 8.16341857847127E-5 0.0 0.0 2.612293945110806E-4 1.306146972555403E-4 18-19 9.796102294165523E-5 0.0 0.0 2.938830688249657E-4 1.632683715694254E-4 20-21 9.796102294165523E-5 0.0 0.0 4.816416961298049E-4 1.7143179014789665E-4 22-23 9.796102294165523E-5 0.0 0.0 0.0015265592741741272 2.0408546446178173E-4 24-25 9.796102294165523E-5 0.0 0.0 0.00399191168487245 2.2857572019719554E-4 26-27 1.1428786009859777E-4 0.0 0.0 0.006922578954543636 2.612293945110806E-4 28-29 1.306146972555403E-4 0.0 0.0 0.015845195460812735 2.612293945110806E-4 30-31 1.4694153441248284E-4 0.0 0.0 0.039412984896859286 2.612293945110806E-4 32-33 1.4694153441248284E-4 0.0 0.0 0.0798708873717629 2.612293945110806E-4 34-35 1.4694153441248284E-4 0.0 0.0 0.12750443477714277 2.775562316680232E-4 36-37 1.4694153441248284E-4 8.16341857847127E-6 0.0 0.19987314047529056 2.775562316680232E-4 38-39 1.5510495299095412E-4 1.632683715694254E-5 0.0 0.309009883450873 2.8571965024649443E-4 40-41 1.632683715694254E-4 1.632683715694254E-5 0.0 0.45071050313597727 3.1020990598190824E-4 42-43 1.632683715694254E-4 1.632683715694254E-5 0.0 0.56913721645386 3.183733245603795E-4 44-45 1.632683715694254E-4 1.632683715694254E-5 0.0 0.6968702269511999 3.3470016171732205E-4 46-47 1.9592204588331046E-4 1.632683715694254E-5 0.0 0.8349462887874629 3.510269988742646E-4 48-49 1.9592204588331046E-4 1.632683715694254E-5 0.0 0.9794877781378752 3.918440917666209E-4 50-51 2.12248883040253E-4 1.632683715694254E-5 0.0 1.1406173340397412 4.0817092892356347E-4 52-53 2.12248883040253E-4 1.632683715694254E-5 0.0 1.3089306982906619 4.6531485897286233E-4 54-55 2.12248883040253E-4 1.632683715694254E-5 0.0 1.4661907937863323 5.224587890221611E-4 56-57 2.12248883040253E-4 1.632683715694254E-5 0.0 1.6415491882704738 5.469490447575751E-4 58-59 2.12248883040253E-4 1.632683715694254E-5 0.0 1.8345732205584269 5.551124633360464E-4 60-61 2.2857572019719554E-4 1.632683715694254E-5 0.0 2.022225723421746 5.714393004929889E-4 62-63 2.2857572019719554E-4 1.632683715694254E-5 0.0 2.2136823793426323 5.714393004929889E-4 64-65 2.2857572019719554E-4 1.632683715694254E-5 0.0 2.4128371389830177 5.714393004929889E-4 66-67 2.2857572019719554E-4 1.632683715694254E-5 0.0 2.6264166592515616 5.714393004929889E-4 68-69 2.3673913877566682E-4 1.632683715694254E-5 0.0 2.8666252509230787 5.714393004929889E-4 70-71 2.4490255735413807E-4 1.632683715694254E-5 0.0 3.1573490767581758 5.877661376499314E-4 72-73 2.4490255735413807E-4 1.632683715694254E-5 0.0 3.4228234489300613 6.775637420131154E-4 74-75 2.4490255735413807E-4 1.632683715694254E-5 0.0 3.677873135577239 6.938905791700579E-4 76-77 2.4490255735413807E-4 1.632683715694254E-5 0.0 3.9536170883208417 7.183808349054717E-4 78-79 2.4490255735413807E-4 1.632683715694254E-5 0.0 4.232120276543968 7.347076720624142E-4 80-81 2.4490255735413807E-4 1.632683715694254E-5 0.0 4.54492614963383 7.918516021117131E-4 82-83 2.4490255735413807E-4 1.632683715694254E-5 0.0 4.893928620700634 8.979760436318397E-4 84-85 2.4490255735413807E-4 1.632683715694254E-5 0.0 5.2302043222036 9.306297179457247E-4 86-87 2.530659759326094E-4 1.632683715694254E-5 0.0 5.566969828821275 9.959370665734949E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 2730 0.0 26.910742 1 ACGTTAA 585 0.0 24.637981 8 GTACATA 1870 0.0 24.119114 1 GTATAAG 3250 0.0 22.748093 1 GTGATCG 2305 0.0 22.59157 8 CGTTAAC 755 0.0 20.323486 1 GTACAAG 3070 0.0 20.2954 1 ATAAGGT 2745 0.0 19.81868 3 TGATCGC 2655 0.0 19.438753 9 TAAGGTG 2760 0.0 19.373556 4 TAGTACT 4180 0.0 19.132544 4 CTAGGCA 4435 0.0 19.080877 4 TAGGCAT 4185 0.0 18.665276 5 CAATGCG 1710 0.0 18.625044 18-19 GTCCTAC 2610 0.0 18.52781 1 TATTCGC 455 0.0 18.393784 9 CGGGCGT 2050 0.0 18.371052 6 GACGTTA 760 0.0 18.353146 7 TACGGGC 2115 0.0 18.246866 4 GGCGTCG 2115 0.0 18.245974 8 >>END_MODULE