FastQCFastQC Report
Fri 27 May 2016
ERR765515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765515_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6124885
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT509870.8324564461210292No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA489420.7990680641350818No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG210870.3442840151284473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160740.2624375804606944No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC152530.24903324715484454No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC146750.23959633527813176No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT143620.23448603524800876No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG138530.22617567513512501No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG131930.21539996261154293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104880.17123586810201336No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA103480.1689501109000414No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC96150.15698253926400252No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC91250.14898238905710065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84340.13770054458165337No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG79680.13009223846651813No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT76190.12439417229874519No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC68100.1111857610387787No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC62990.10284274725158105No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6350.032.222862
TCACGCA8650.023.6539654
TCTAACG11300.020.988282
AGTACTT275200.020.95392612-13
ATCACGC9950.020.5634963
GTACTAG28050.019.9078481
CTAACGC11500.019.81373
GTATAAC10150.019.7141631
TAGGCAT38750.019.0806335
GTATAAG27450.018.4782181
TAGTACT39100.018.4341144
TACTTTT318500.018.3678414-15
CTAGGCA45400.018.3343094
GAGTACT262750.018.2565512-13
TAACGCA5150.018.0588514
ATACGGG22300.017.7248043
TATTCGC4003.092282E-1117.4385059
CGATAAT19850.017.33502266-67
CATGGGG125000.017.224174
TACGGGC22050.017.08224
Produced by FastQC (version 0.11.5)