FastQCFastQC Report
Fri 27 May 2016
ERR765514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7804532
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA192580.24675406545837725No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT175560.22494622355318677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG22550.022.2705541
GTACATA21700.019.5000361
GTACAAG42700.018.5129151
GTATCAA423150.018.1099241
TACATGA36400.018.012052
GTACATG615550.017.9564151
CATGGGG197600.017.8137114
CTAACGC9600.017.4371993
TACATGG605050.017.3070322
TGATCGC15350.016.9638759
GTATAAG27450.016.9399221
GTACAAA46800.016.592981
TAGTACT29100.015.9791064
ACATGGG585950.015.9428083
CTAGTAC29250.015.4202473
CATGGGT54500.015.35753254
ATAGGAC59550.015.3045223
AACGCAG463200.015.2286876
GTATTAG30600.014.8921851
TATCAAC514250.014.8291062
Produced by FastQC (version 0.11.5)