##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765514_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7804532 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.351872860537956 33.0 33.0 33.0 27.0 33.0 2 31.225348041368783 33.0 33.0 33.0 27.0 33.0 3 31.279746178246178 33.0 33.0 33.0 27.0 33.0 4 34.86103894506423 37.0 37.0 37.0 33.0 37.0 5 34.986348316593485 37.0 37.0 37.0 33.0 37.0 6 34.94799521611289 37.0 37.0 37.0 33.0 37.0 7 34.831489447413375 37.0 37.0 37.0 33.0 37.0 8 34.74612045924086 37.0 37.0 37.0 33.0 37.0 9 34.66234234160357 37.0 37.0 37.0 27.0 37.0 10-11 34.67005145215626 37.0 37.0 37.0 27.0 37.0 12-13 34.61411401734274 37.0 37.0 37.0 27.0 37.0 14-15 36.072923206670175 37.0 37.0 40.0 27.0 40.0 16-17 36.13931258145908 37.0 37.0 40.0 27.0 40.0 18-19 36.16363838344182 37.0 37.0 40.0 27.0 40.0 20-21 36.180913794702874 37.0 37.0 40.0 27.0 40.0 22-23 36.23874327121729 37.0 37.0 40.0 27.0 40.0 24-25 36.21386452128071 37.0 37.0 40.0 27.0 40.0 26-27 36.23501120887197 37.0 37.0 40.0 27.0 40.0 28-29 36.16276164925712 37.0 37.0 40.0 27.0 40.0 30-31 36.13205192828987 37.0 37.0 40.0 27.0 40.0 32-33 36.0001857254221 37.0 37.0 40.0 27.0 40.0 34-35 35.94363050853018 37.0 37.0 40.0 27.0 40.0 36-37 35.82811679162825 37.0 37.0 40.0 27.0 40.0 38-39 35.616922257478095 37.0 37.0 40.0 27.0 40.0 40-41 35.53096636672129 37.0 33.0 40.0 27.0 40.0 42-43 35.395418392800494 37.0 33.0 40.0 27.0 40.0 44-45 35.264980270437746 37.0 33.0 40.0 27.0 40.0 46-47 35.14765593888269 37.0 33.0 40.0 27.0 40.0 48-49 34.991393141830926 37.0 33.0 40.0 27.0 40.0 50-51 34.829468378116715 37.0 33.0 40.0 27.0 40.0 52-53 34.65226889965984 37.0 33.0 40.0 27.0 40.0 54-55 34.51790997845867 37.0 33.0 40.0 27.0 40.0 56-57 34.33077915498329 37.0 33.0 40.0 27.0 40.0 58-59 34.11192791572896 37.0 33.0 37.0 27.0 40.0 60-61 33.88254465482363 37.0 33.0 37.0 27.0 40.0 62-63 33.70655639569419 37.0 33.0 37.0 27.0 40.0 64-65 33.40758651511712 37.0 33.0 37.0 22.0 40.0 66-67 33.1893059058506 37.0 33.0 37.0 22.0 40.0 68-69 32.89823220662046 37.0 33.0 37.0 22.0 40.0 70-71 32.75535567026953 37.0 33.0 37.0 22.0 37.0 72-73 32.490821935255056 35.0 33.0 37.0 22.0 37.0 74-75 32.21754501102693 33.0 33.0 37.0 22.0 37.0 76-77 32.03174834826739 33.0 33.0 37.0 22.0 37.0 78-79 31.8042798081935 33.0 33.0 37.0 22.0 37.0 80-81 31.54857171448589 33.0 33.0 37.0 22.0 37.0 82-83 31.383829997750027 33.0 30.0 37.0 22.0 37.0 84-85 31.204565693368927 33.0 27.0 37.0 22.0 37.0 86-87 31.00381047832208 33.0 27.0 37.0 18.5 37.0 88-89 30.82985187324493 33.0 27.0 37.0 15.0 37.0 90-91 30.64566043165689 33.0 27.0 37.0 15.0 37.0 92-93 30.506719685434053 33.0 27.0 37.0 15.0 37.0 94-95 30.273307291199522 33.0 27.0 37.0 15.0 37.0 96-97 30.19768379449274 33.0 27.0 37.0 15.0 37.0 98-99 29.053952370238214 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3391777371288818 1101 2 0.35045435843478145 1101 3 0.41091889867535514 1101 4 0.44775116481850574 1101 5 0.42812983758152967 1101 6 0.3105131604736684 1101 7 0.2628872709628496 1101 8 0.31518845765916126 1101 9 0.3313864551699268 1101 10-11 0.3362481616916071 1101 12-13 0.3896573645284249 1101 14-15 0.4847566346416059 1101 16-17 0.46254595333917337 1101 18-19 0.42905046196980123 1101 20-21 0.3994369691348396 1101 22-23 0.39002208897352375 1101 24-25 0.4573261649348268 1101 26-27 0.4560833564506339 1101 28-29 0.37515916981870845 1101 30-31 0.4422684687228511 1101 32-33 0.5075467722766689 1101 34-35 0.4672856811437569 1101 36-37 0.46615718666372175 1101 38-39 0.4291487986757829 1101 40-41 0.4217446464083494 1101 42-43 0.36687686832026856 1101 44-45 0.4486653959021254 1101 46-47 0.4569228468186921 1101 48-49 0.4246374662379111 1101 50-51 0.38563352807467766 1101 52-53 0.33135890104084353 1101 54-55 0.40743177468198866 1101 56-57 0.35322939219773986 1101 58-59 0.3521147182099611 1101 60-61 0.43597648332765004 1101 62-63 0.45789458317777587 1101 64-65 0.3220309393037226 1101 66-67 0.3462876385434299 1101 68-69 0.33321455776776077 1101 70-71 0.3596894113105691 1101 72-73 0.4067748686112296 1101 74-75 0.3796584651651429 1101 76-77 0.3104086096169567 1101 78-79 0.39674517386728425 1101 80-81 0.3158313909328321 1101 82-83 0.33870814561166895 1101 84-85 0.2618014600093055 1101 86-87 0.29557085967141106 1101 88-89 0.3718793203715336 1101 90-91 0.33298481574971817 1101 92-93 0.41329197408475693 1101 94-95 0.41550786374130766 1101 96-97 0.362956082354259 1101 98-99 0.22483925732877807 1102 1 0.2746990856379341 1102 2 0.27427380036888493 1102 3 0.2888774363481268 1102 4 0.32456714426210453 1102 5 0.22777235643576432 1102 6 0.17431116317284534 1102 7 0.21191272868068722 1102 8 0.2219861484522525 1102 9 0.2216213051057423 1102 10-11 0.16089952333536672 1102 12-13 0.25782764274470793 1102 14-15 0.2836674837382418 1102 16-17 0.332726975537355 1102 18-19 0.33278913755003003 1102 20-21 0.34033194369344244 1102 22-23 0.2439957458866644 1102 24-25 0.34832643983760647 1102 26-27 0.35349201329263025 1102 28-29 0.1881131766482369 1102 30-31 0.25064114311560814 1102 32-33 0.30811352688217397 1102 34-35 0.2701772291251032 1102 36-37 0.2887142154381266 1102 38-39 0.2861254576297938 1102 40-41 0.2815554171940775 1102 42-43 0.25440883412791493 1102 44-45 0.28150639886389683 1102 46-47 0.3220680491641943 1102 48-49 0.32427063777898013 1102 50-51 0.2874625411683951 1102 52-53 0.2617950637162352 1102 54-55 0.32157843415068044 1102 56-57 0.3368689978956496 1102 58-59 0.31818096639158 1102 60-61 0.3660405248333447 1102 62-63 0.2840957125506307 1102 64-65 0.23490987817133657 1102 66-67 0.3365135978152267 1102 68-69 0.3198085286911123 1102 70-71 0.3062552168518664 1102 72-73 0.33138991118168093 1102 74-75 0.23454553082589769 1102 76-77 0.2458219458752069 1102 78-79 0.32081603429353933 1102 80-81 0.3512923873665805 1102 82-83 0.247822038148108 1102 84-85 0.18289365337434305 1102 86-87 0.31893050306555537 1102 88-89 0.33423064682973447 1102 90-91 0.2743428214822252 1102 92-93 0.3316658161735404 1102 94-95 0.3216667318571744 1102 96-97 0.27828661575079394 1102 98-99 0.2992959649138598 1103 1 0.04597451760608706 1103 2 0.10463281111668721 1103 3 0.10815678495267633 1103 4 0.12778241280105362 1103 5 0.05864113091994483 1103 6 0.11455391472485843 1103 7 0.015260561335246337 1103 8 0.0474207700404321 1103 9 0.11036071285619897 1103 10-11 0.05134600171635384 1103 12-13 0.04745249555369213 1103 14-15 0.062000643572233116 1103 16-17 -0.012040516834055381 1103 18-19 0.05926717254786951 1103 20-21 0.04785503582423445 1103 22-23 0.09167602885045056 1103 24-25 0.179536802808272 1103 26-27 0.23833346560581248 1103 28-29 0.14640736141243593 1103 30-31 0.15588978446078983 1103 32-33 0.2406486025220005 1103 34-35 0.1315884744826974 1103 36-37 0.17972208401350542 1103 38-39 0.14494542073657612 1103 40-41 0.1379889248562236 1103 42-43 0.07891897976374906 1103 44-45 0.0457621931750154 1103 46-47 0.02163816616015879 1103 48-49 0.06785234252178896 1103 50-51 0.07032644111039588 1103 52-53 0.08725785562744193 1103 54-55 0.11580739475817836 1103 56-57 0.003697328205440442 1103 58-59 0.06463830789778058 1103 60-61 0.11350305392677029 1103 62-63 0.12580899945157853 1103 64-65 0.04179891990420259 1103 66-67 0.08109021149326878 1103 68-69 0.08756111682339451 1103 70-71 0.008408913388052497 1103 72-73 0.08241734586176364 1103 74-75 0.06368449607698068 1103 76-77 -0.042045354309451 1103 78-79 0.0020888807053474068 1103 80-81 -0.03266326399199215 1103 82-83 0.059650784770212795 1103 84-85 0.07584480425473927 1103 86-87 0.1738195221817982 1103 88-89 0.1623070506008446 1103 90-91 0.05236664949991976 1103 92-93 0.08838435928652189 1103 94-95 0.0015998842403135427 1103 96-97 -0.11946307627711406 1103 98-99 0.011051888452573877 1104 1 0.10766335011737738 1104 2 0.10322722019518693 1104 3 0.05403943280100165 1104 4 0.0820968476349222 1104 5 0.08374784162473503 1104 6 0.08770004105107176 1104 7 -0.008988637826917056 1104 8 0.01698110472299419 1104 9 0.0052373794835958165 1104 10-11 0.08242268510567641 1104 12-13 0.011292824496827336 1104 14-15 0.07542911734752522 1104 16-17 0.14756240004911803 1104 18-19 0.12773168731397533 1104 20-21 0.09407821489108414 1104 22-23 0.09073736338649496 1104 24-25 0.06001124868408425 1104 26-27 0.09865266940938255 1104 28-29 0.02201805852079275 1104 30-31 0.10360515985519925 1104 32-33 0.18871879980255812 1104 34-35 0.11937274160356282 1104 36-37 0.12967873019833576 1104 38-39 0.1578778422846625 1104 40-41 0.17197214103148184 1104 42-43 0.07262177859861652 1104 44-45 0.04073677278590537 1104 46-47 0.01585603594124052 1104 48-49 0.08818488294741655 1104 50-51 0.16073995517091078 1104 52-53 -4.3248597148703993E-4 1104 54-55 0.18342233644717254 1104 56-57 0.07252708387026985 1104 58-59 0.14924906817073236 1104 60-61 0.09770348810686613 1104 62-63 0.09528920245555383 1104 64-65 -0.005011080111813726 1104 66-67 -0.002391817650860162 1104 68-69 -0.029072544746597373 1104 70-71 0.026573759480911008 1104 72-73 0.054889846388185504 1104 74-75 -0.06570532165328302 1104 76-77 -0.15143239783017037 1104 78-79 -0.035379875839151254 1104 80-81 -0.005097426841235375 1104 82-83 0.043968190684708475 1104 84-85 -0.08159738098399316 1104 86-87 -0.008878231606335163 1104 88-89 0.061733962732347436 1104 90-91 0.146121885813276 1104 92-93 0.13518175283344647 1104 94-95 -0.050779165570904894 1104 96-97 -0.22729801218206802 1104 98-99 -0.09084903912620845 1105 1 0.06131970542192988 1105 2 0.1289097984807448 1105 3 0.13193086429876644 1105 4 0.06821475177174818 1105 5 0.055780950820967234 1105 6 0.055454469616528 1105 7 0.026124509695179654 1105 8 0.021403084417030982 1105 9 -0.02764346524141814 1105 10-11 -0.008930154452805539 1105 12-13 0.045856683862197656 1105 14-15 0.10567286629878936 1105 16-17 0.040744984315260524 1105 18-19 0.07657333079211526 1105 20-21 0.13039554587152224 1105 22-23 0.06213687599168338 1105 24-25 0.08114317216404743 1105 26-27 0.12399314761346147 1105 28-29 0.06736199521112951 1105 30-31 0.12295116349230995 1105 32-33 0.09163271971991804 1105 34-35 0.07042451343983913 1105 36-37 0.09055233673651486 1105 38-39 -0.017063016859822255 1105 40-41 0.02077741828737345 1105 42-43 0.06067429484672715 1105 44-45 -0.01288407137749914 1105 46-47 -0.052228601311284706 1105 48-49 -0.082544844126069 1105 50-51 0.048928306855898995 1105 52-53 -0.05693346900861229 1105 54-55 0.10412611205816802 1105 56-57 0.15172685354374238 1105 58-59 -0.010721129155498943 1105 60-61 0.09094999555975392 1105 62-63 0.10017722271366836 1105 64-65 -0.055840486717137594 1105 66-67 0.023014724646621687 1105 68-69 -0.018959523243950116 1105 70-71 -0.12570914475217876 1105 72-73 0.04943860463668415 1105 74-75 0.04910300148553404 1105 76-77 -0.059527931279461654 1105 78-79 -0.001646078807407747 1105 80-81 0.0028741255429238777 1105 82-83 0.12359640868029587 1105 84-85 0.09638717888546466 1105 86-87 0.10557375552527404 1105 88-89 0.12144646239202217 1105 90-91 0.1335095818383074 1105 92-93 0.14437287800000576 1105 94-95 0.08929888688782128 1105 96-97 -0.03976009243193346 1105 98-99 0.11913467075988038 1106 1 0.04756876360619344 1106 2 0.09866402405418029 1106 3 0.05725314463441933 1106 4 -0.027265388412921254 1106 5 0.009366139174090904 1106 6 -0.016660499352262548 1106 7 0.02469078233724531 1106 8 0.004951878115235786 1106 9 -0.06507475510598937 1106 10-11 -0.04259797767008422 1106 12-13 -0.03427025398378447 1106 14-15 -0.03027932304200931 1106 16-17 -0.04135671027674448 1106 18-19 -0.0303447934040193 1106 20-21 -0.07692320886705772 1106 22-23 -0.02957090363621262 1106 24-25 0.027725456508242985 1106 26-27 -0.008642991125888955 1106 28-29 -0.05861624894680517 1106 30-31 -0.024025118612975405 1106 32-33 0.05505301041063149 1106 34-35 -0.0664868092578601 1106 36-37 -0.07029272888259186 1106 38-39 -0.1412631176223016 1106 40-41 -0.06809154896914293 1106 42-43 -0.09585250531335276 1106 44-45 -0.15588274592472828 1106 46-47 -0.19210031886442636 1106 48-49 -0.2156926967063697 1106 50-51 -0.20233985949208488 1106 52-53 -0.17635142603187148 1106 54-55 -0.17731067760847452 1106 56-57 -0.0773195832615059 1106 58-59 -0.05398246517533778 1106 60-61 -0.049564748526030655 1106 62-63 -0.09436649464081626 1106 64-65 -0.19728242173424348 1106 66-67 -0.13921490371490108 1106 68-69 -0.12471182698338623 1106 70-71 -0.15611769889002858 1106 72-73 -0.08627201598975631 1106 74-75 -0.05309577073576577 1106 76-77 -0.138780743220984 1106 78-79 -0.06466834223734708 1106 80-81 -0.1629833017775404 1106 82-83 -0.10258368731659928 1106 84-85 -0.19710480365593241 1106 86-87 -0.2101407338650425 1106 88-89 -0.048694935222663105 1106 90-91 -0.1700723310715162 1106 92-93 -0.16039849380248938 1106 94-95 -0.12441555346280708 1106 96-97 -0.20827282887468002 1106 98-99 0.11047309600850141 1107 1 -0.011079226732476855 1107 2 0.07881931626774019 1107 3 0.027666092372044204 1107 4 -0.02787905626540521 1107 5 5.770808595713106E-4 1107 6 -0.07908566089067648 1107 7 -0.10748944350300604 1107 8 -0.08012216634747205 1107 9 -0.1999869248171322 1107 10-11 -0.08956005198253081 1107 12-13 -0.13901798509900232 1107 14-15 -0.11283413279836907 1107 16-17 -0.0744211990330399 1107 18-19 -0.1452314841925073 1107 20-21 -0.116841695327075 1107 22-23 -0.0918513792031348 1107 24-25 0.058337924636482796 1107 26-27 0.07086478718557032 1107 28-29 0.04413097128183807 1107 30-31 -0.017728452029750486 1107 32-33 -0.11478446307722834 1107 34-35 -0.11314949756248183 1107 36-37 -0.1471131357294695 1107 38-39 -0.09283194478769019 1107 40-41 -0.1596224071881167 1107 42-43 -0.21708721946796317 1107 44-45 -0.10002223045957948 1107 46-47 -0.22342893820255227 1107 48-49 -0.11428922653295359 1107 50-51 -0.06663386016739992 1107 52-53 -0.16174928432063496 1107 54-55 -0.057739395094600354 1107 56-57 -0.0608978338311843 1107 58-59 -0.045488381669692046 1107 60-61 0.008135429871252597 1107 62-63 -0.21226099004207555 1107 64-65 -0.2844325971533692 1107 66-67 -0.043360406980326616 1107 68-69 -0.09204597113323132 1107 70-71 -0.023376289324204436 1107 72-73 -0.0035731567203853842 1107 74-75 -0.05955338683596523 1107 76-77 -0.15733650070465188 1107 78-79 -0.020765737393556805 1107 80-81 -0.236869001945486 1107 82-83 -0.14776606459641783 1107 84-85 -0.19089428579085777 1107 86-87 -0.22019115133000966 1107 88-89 -0.11759046572885268 1107 90-91 -0.08462460761478496 1107 92-93 -0.09834739042486262 1107 94-95 -0.0892523970260406 1107 96-97 -0.2579565113785023 1107 98-99 0.1387913479728411 1108 1 -0.10198748486124387 1108 2 -0.05001914240087402 1108 3 -0.03532433496621934 1108 4 -0.0982738668493397 1108 5 -0.03127430854821256 1108 6 -0.17385934874123876 1108 7 -0.15450046119463678 1108 8 -0.2165771891846191 1108 9 -0.08056660277529915 1108 10-11 -0.17333262946402073 1108 12-13 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-0.06205177046950183 2215 74-75 0.09485264986326314 2215 76-77 0.04445109924222379 2215 78-79 0.07270131366646737 2215 80-81 0.16512387705148157 2215 82-83 0.05101060420781778 2215 84-85 0.05758060965201395 2215 86-87 0.05120541033329218 2215 88-89 -0.08643503818460374 2215 90-91 0.1639109274483097 2215 92-93 0.09597131993043462 2215 94-95 0.12821957534463735 2215 96-97 0.1321173613153661 2215 98-99 0.12113942064006267 2216 1 -0.042430667726971905 2216 2 -0.037274539501865434 2216 3 -0.03361371084448095 2216 4 -0.01791329052207402 2216 5 -0.09041362963473887 2216 6 -0.03299829933498444 2216 7 -0.015295075470959318 2216 8 -0.022924286298781738 2216 9 0.025255596884015574 2216 10-11 0.002758677929044495 2216 12-13 -0.023874390170725235 2216 14-15 -0.11281497645293825 2216 16-17 -0.06425789902638712 2216 18-19 -0.07915323975038291 2216 20-21 -0.1267954385228336 2216 22-23 -0.21759220387868083 2216 24-25 -0.15726661134872444 2216 26-27 -0.18697812666943747 2216 28-29 -0.068871192411045 2216 30-31 -0.12477308521157227 2216 32-33 -0.07475114512392622 2216 34-35 -0.16943602594214013 2216 36-37 -0.20394418570096207 2216 38-39 -0.12050460911797245 2216 40-41 -0.15186210001468936 2216 42-43 -0.18068480979572854 2216 44-45 -0.12183183014998633 2216 46-47 -0.04213004182565783 2216 48-49 0.002124948750228839 2216 50-51 -0.007291515520520875 2216 52-53 0.11021346272315924 2216 54-55 0.022208555996826362 2216 56-57 0.15011377125733105 2216 58-59 -0.021377541682198853 2216 60-61 0.060999545703218416 2216 62-63 0.0055752676797879985 2216 64-65 0.12041551508814763 2216 66-67 0.014092323500889847 2216 68-69 -0.03424781196840598 2216 70-71 -0.021361791732445568 2216 72-73 -0.08753925242884719 2216 74-75 0.04169029070828856 2216 76-77 -0.006296363302190855 2216 78-79 0.01694532853030495 2216 80-81 0.18553327067389702 2216 82-83 0.08831302876765079 2216 84-85 0.09987015802016685 2216 86-87 0.03802762075310184 2216 88-89 0.05911650088096465 2216 90-91 0.21686236900476885 2216 92-93 0.3546671478295522 2216 94-95 0.28278672241943426 2216 96-97 0.26374954333411793 2216 98-99 0.30505948212447365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 195.0 8 1324.0 9 2555.0 10 4206.0 11 8249.0 12 15949.0 13 28333.0 14 40765.0 15 44180.0 16 45893.0 17 47590.0 18 48805.0 19 50772.0 20 53649.0 21 57959.0 22 63767.0 23 71073.0 24 80243.0 25 89854.0 26 99517.0 27 112031.0 28 131291.0 29 161569.0 30 202981.0 31 256656.0 32 339283.0 33 455702.0 34 624365.0 35 905569.0 36 1403872.0 37 1771053.0 38 584719.0 39 560.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.050216039523555 18.617044654147765 12.554727428607526 24.778011877721156 2 16.039847104220982 20.957310444751844 37.56758252769033 25.43525992333685 3 18.15988103316434 26.576187567012383 28.65729996740354 26.606631432419743 4 12.918865602703661 17.45416637410161 36.115682529074135 33.5112854941206 5 14.490926553956085 37.61646438248955 32.78206816244715 15.110540901107203 6 31.717865978382815 36.70697999572556 17.590420540270703 13.984733485620918 7 27.95031143443322 32.15746953180537 21.688449736640198 18.203769297121212 8 27.020825848366055 33.40293819027201 19.813756929947882 19.762479031414053 9 27.172583826935426 15.925118892458896 19.01149229703972 37.89080498356596 10-11 25.40788480334247 25.986740780869372 26.427689706442358 22.1776847093458 12-13 26.140632135277297 24.078573833767354 26.08133966264729 23.699454368308054 14-15 24.38346078919274 25.016913249891214 23.365039697447585 27.234586263468458 16-17 24.15608803680272 27.197750435115804 24.36308957174431 24.28307195633716 18-19 24.646250409377526 26.59593169712162 25.563480295807615 23.194337597693238 20-21 26.246023047855527 25.790625549963124 24.290407823024676 23.672943579156666 22-23 25.28664114645183 25.702643028435272 24.25817460931674 24.752541215796157 24-25 24.483746574194065 26.414402886758648 24.378380037224897 24.723470501822387 26-27 24.858864018717338 26.142342972606937 24.341491415882828 24.657301592792905 28-29 24.424363797658994 26.055747629171723 24.988693103437008 24.531195469732275 30-31 24.82157692936522 26.169116369512707 24.63183985001443 24.377466851107645 32-33 24.32008683166665 26.348076744186642 24.18276266873863 25.14907375540808 34-35 24.7631118148623 26.380955102206077 24.75046530692575 24.10546777600587 36-37 24.671396055522614 26.373644185199062 24.426595982949394 24.52836377632893 38-39 24.82343751191215 26.023221252934913 24.40823001355046 24.745111221602475 40-41 24.532361453575948 26.472766080016076 24.761369419716647 24.233503046691332 42-43 25.070817827385422 26.15743006755562 24.56900682834025 24.20274527671871 44-45 24.43712832492711 26.439932593011346 24.45841723757427 24.66452184448728 46-47 24.067516155997566 26.41702923378365 25.08873049658839 24.42672411363039 48-49 24.609817126974026 26.528386426649142 24.638480028255444 24.223316418121385 50-51 23.90154848490595 26.773764269273286 24.528293304454387 24.796393941366375 52-53 24.047098531981163 26.32657537953589 24.478149362447358 25.148176726035594 54-55 24.44532228197668 26.1385179790409 24.64533427500842 24.77082546397401 56-57 24.52386638942604 25.802386357055106 24.32883227335092 25.344914980167932 58-59 24.33663543182346 25.78050804327537 24.865046360243 25.017810164658176 60-61 24.385062422705168 26.066931367569506 24.71508861774159 24.832917591983737 62-63 23.910882805016367 26.163183135132257 24.552657353445408 25.373276706405974 64-65 24.130396287695405 25.858116796753478 24.648435037488476 25.36305187806264 66-67 24.69941333426954 25.90958878430788 24.455888083964574 24.935109797458 68-69 24.8249863028302 25.589029553597832 24.410438704076043 25.175545439495924 70-71 24.138333983382985 25.771506862935535 24.693908616173267 25.39625053750821 72-73 24.739151559632276 25.812752129147526 24.619458283981665 24.828638027238533 74-75 25.05639031270549 25.63074249679545 24.629554981644 24.68331220885506 76-77 24.62906167852217 25.855394019782352 24.8379275016106 24.677616800084873 78-79 24.6986814840403 25.79303281734254 24.894926435050817 24.61335926356635 80-81 24.38241011760859 25.583616032325835 25.267152469872634 24.766821380192944 82-83 24.69190977754976 25.718326223788946 24.705760704165222 24.884003294496072 84-85 24.727248219367926 25.771711872025126 24.466367746329954 25.034672162276994 86-87 24.60077042415868 25.375935418036597 24.756846406677557 25.266447751127163 88-89 24.66026790587828 25.03367274296524 24.991287113692405 25.314772237464076 90-91 24.984810107768155 25.221051050850967 24.77279867646132 25.021340164919565 92-93 24.728164353737032 25.42431756317996 24.671908578246587 25.175609504836423 94-95 24.24414429974789 25.524156989810535 25.073694361173736 25.158004349267838 96-97 24.79146731668215 25.49059956445819 24.631867740435943 25.086065378423715 98-99 24.509765607982644 25.63852643566584 24.562440130939304 25.28926782541221 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2045.0 1 1733.0 2 1439.0 3 1461.0 4 1347.5 5 1174.0 6 1079.5 7 1023.0 8 972.5 9 952.0 10 898.0 11 822.0 12 827.5 13 852.0 14 902.5 15 1074.0 16 1290.5 17 1508.5 18 1888.5 19 2574.0 20 3633.5 21 4987.0 22 6801.0 23 9190.5 24 12511.0 25 16923.0 26 22668.5 27 28882.5 28 36302.5 29 45781.5 30 54346.0 31 64419.0 32 76208.0 33 87682.5 34 97786.0 35 107636.0 36 116072.0 37 127488.5 38 141523.0 39 145616.0 40 149660.5 41 159284.5 42 173025.5 43 195429.0 44 219017.5 45 242934.5 46 282107.5 47 316438.5 48 337694.0 49 359878.0 50 383168.5 51 373481.0 52 349291.5 53 345291.0 54 332097.5 55 320770.0 56 302667.0 57 284830.5 58 269948.0 59 238961.5 60 198930.5 61 152386.5 62 122072.5 63 97907.0 64 73400.5 65 58643.5 66 43790.5 67 32327.5 68 25344.5 69 21371.0 70 19131.0 71 17333.5 72 17058.0 73 15570.0 74 11484.5 75 8480.5 76 6528.5 77 4614.5 78 2990.0 79 2099.0 80 1553.5 81 1096.5 82 833.0 83 660.5 84 464.5 85 349.0 86 235.5 87 159.5 88 109.0 89 73.5 90 51.5 91 45.0 92 37.5 93 28.0 94 19.0 95 16.5 96 14.5 97 8.5 98 7.0 99 17.5 100 27.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0022166607811973864 2 0.0 3 5.1252272397627434E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.406534049703429E-6 18-19 0.0 20-21 4.03611645131316E-4 22-23 0.0 24-25 0.004439728096444476 26-27 2.5626136198813717E-5 28-29 0.008539909888254671 30-31 6.150272687715291E-4 32-33 7.687840859644114E-5 34-35 7.687840859644114E-5 36-37 0.0 38-39 6.406534049703429E-6 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.3704175983902686E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.203267024851714E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7804532.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.8883118829492 #Duplication Level Percentage of deduplicated Percentage of total 1 75.7465687802042 32.4864246590866 2 13.536175638843256 11.610874450021775 3 4.499168045232302 5.788851670131656 4 1.954235110209268 3.352553795970588 5 1.0334995009598864 2.216252446401998 6 0.6594227891690719 1.6968918146764453 7 0.4435761684807172 1.3316963140351235 8 0.3041649451457115 1.043609682501553 9 0.223049143311186 0.8609581101199301 >10 1.3306722001550593 10.75463499733785 >50 0.13087596378129368 3.904849519922402 >100 0.1120767644386651 9.890540452513683 >500 0.01496733871986933 4.482851945918202 >1k 0.011007723027653573 8.879594940512447 >5k 4.7990073056121895E-4 1.225846066474355 >10k+ 5.998759132015237E-5 0.473569134375584 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 19258 0.24675406545837725 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 17556 0.22494622355318677 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5626136198813714E-5 2 0.0 0.0 0.0 0.0 2.5626136198813714E-5 3 0.0 0.0 0.0 1.2813068099406857E-5 2.5626136198813714E-5 4 0.0 0.0 0.0 5.125227239762743E-5 2.5626136198813714E-5 5 2.5626136198813714E-5 0.0 0.0 6.406534049703429E-5 2.5626136198813714E-5 6 3.8439204298220575E-5 0.0 0.0 7.687840859644115E-5 6.406534049703429E-5 7 3.8439204298220575E-5 0.0 0.0 7.687840859644115E-5 6.406534049703429E-5 8 3.8439204298220575E-5 0.0 0.0 1.0250454479525485E-4 6.406534049703429E-5 9 3.8439204298220575E-5 0.0 0.0 1.6656988529228914E-4 1.0250454479525485E-4 10-11 7.047187454673772E-5 0.0 0.0 1.986025555408063E-4 1.2813068099406857E-4 12-13 1.0250454479525487E-4 0.0 0.0 2.2422869173962E-4 1.5375681719288227E-4 14-15 1.2813068099406857E-4 1.2813068099406857E-5 0.0 2.3063522578932344E-4 2.1782215768991657E-4 16-17 1.4735028314317886E-4 1.2813068099406857E-5 0.0 2.6266789603784056E-4 2.3704175983902689E-4 18-19 1.6656988529228914E-4 1.9219602149110284E-5 0.0 2.947005662863577E-4 2.754809641372474E-4 20-21 1.9219602149110284E-4 2.5626136198813714E-5 0.0 5.637749963739017E-4 2.947005662863577E-4 22-23 2.2422869173962E-4 2.5626136198813714E-5 0.0 0.0018514883403642907 3.0751363438576455E-4 24-25 2.434482938887303E-4 3.203267024851714E-5 0.0 0.004894592013973419 3.2032670248517144E-4 26-27 2.5626136198813714E-4 3.8439204298220575E-5 0.0 0.008591162160652298 3.2673323653487486E-4 28-29 2.69074430087544E-4 3.8439204298220575E-5 0.0 0.022192233948172678 3.331397705845783E-4 30-31 2.754809641372474E-4 3.8439204298220575E-5 0.0 0.060528933701597995 3.331397705845783E-4 32-33 2.818874981869509E-4 3.8439204298220575E-5 0.0 0.12271715972206917 4.3564431537983315E-4 34-35 3.011071003360612E-4 4.4845738347924E-5 0.0 0.20344589528238208 4.8049005372775714E-4 36-37 3.2032670248517144E-4 5.765880644733085E-5 0.0 0.3252789533055922 5.445553942247914E-4 38-39 3.2032670248517144E-4 6.406534049703429E-5 0.0 0.503867496475125 5.829945985230121E-4 40-41 3.2032670248517144E-4 7.687840859644115E-5 0.0 0.7154240638644316 5.894011325727154E-4 42-43 3.3313977058457833E-4 7.687840859644115E-5 0.0 0.9108169458463364 6.150272687715292E-4 44-45 3.4595283868398517E-4 7.687840859644115E-5 0.0 1.1370188500732652 6.150272687715292E-4 46-47 3.4595283868398517E-4 7.687840859644115E-5 0.0 1.3866174166497107 6.342468709206394E-4 48-49 3.4595283868398517E-4 7.687840859644115E-5 0.0 1.6429748766485934 7.047187454673772E-4 50-51 3.4595283868398517E-4 7.687840859644115E-5 0.0 1.923311993595516 7.17531813566784E-4 52-53 3.4595283868398517E-4 8.9691476695848E-5 0.0 2.2077813250044973 7.62377551914708E-4 54-55 3.4595283868398517E-4 9.609801074555142E-5 0.0 2.485722398216831 8.264428924117423E-4 56-57 3.4595283868398517E-4 1.0250454479525485E-4 0.0 2.800981532268687 8.584755626602595E-4 58-59 3.4595283868398517E-4 1.3453721504377202E-4 0.0 3.1452878917018983 8.9691476695848E-4 60-61 3.4595283868398517E-4 1.4735028314317886E-4 0.0 3.489062508808984 9.353539712567006E-4 62-63 3.58765906783392E-4 1.537568171928823E-4 0.0 3.837443423897807 9.41760505306404E-4 64-65 3.58765906783392E-4 1.537568171928823E-4 0.0 4.201610038885099 9.609801074555143E-4 66-67 3.7157897488279885E-4 1.537568171928823E-4 0.0 4.59647035850452 9.609801074555143E-4 68-69 3.779855089325023E-4 1.537568171928823E-4 0.0 5.024426833024709 9.609801074555143E-4 70-71 4.1001817918101947E-4 1.537568171928823E-4 0.0 5.498119554125731 9.86606243654328E-4 72-73 4.228312472804263E-4 1.537568171928823E-4 0.0 5.95547561340001 0.0010570781182010656 74-75 4.228312472804263E-4 1.6656988529228914E-4 0.0 6.411575992000545 0.0010955173224992862 76-77 4.228312472804263E-4 1.6656988529228914E-4 0.0 6.901246609021527 0.0011339565267975069 78-79 4.228312472804263E-4 1.6656988529228914E-4 0.0 7.404607989306726 0.0011467695948969136 80-81 4.3564431537983315E-4 1.6656988529228914E-4 0.0 7.953993910204993 0.0011788022651454308 82-83 4.3564431537983315E-4 1.79382953391696E-4 0.0 8.532305332337673 0.0012300545375430582 84-85 4.3564431537983315E-4 1.79382953391696E-4 0.0 9.100167697435285 0.0012428676056424651 86-87 4.3564431537983315E-4 1.79382953391696E-4 0.0 9.681733638865213 0.001255680673741872 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 2255 0.0 22.270554 1 GTACATA 2170 0.0 19.500036 1 GTACAAG 4270 0.0 18.512915 1 GTATCAA 42315 0.0 18.109924 1 TACATGA 3640 0.0 18.01205 2 GTACATG 61555 0.0 17.956415 1 CATGGGG 19760 0.0 17.813711 4 CTAACGC 960 0.0 17.437199 3 TACATGG 60505 0.0 17.307032 2 TGATCGC 1535 0.0 16.963875 9 GTATAAG 2745 0.0 16.939922 1 GTACAAA 4680 0.0 16.59298 1 TAGTACT 2910 0.0 15.979106 4 ACATGGG 58595 0.0 15.942808 3 CTAGTAC 2925 0.0 15.420247 3 CATGGGT 5450 0.0 15.3575325 4 ATAGGAC 5955 0.0 15.304522 3 AACGCAG 46320 0.0 15.228687 6 GTATTAG 3060 0.0 14.892185 1 TATCAAC 51425 0.0 14.829106 2 >>END_MODULE