FastQCFastQC Report
Fri 27 May 2016
ERR765514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765514_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7804532
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254710.3263616575599921No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT191890.2458699637595182No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA185090.23715707745192152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162270.20791765604907508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137040.17559028523427156No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA536300.028.6910631
TATCACG9000.028.4181292
ATCAACG660450.023.1860683
CAACGCA661200.023.14575
AGTACTT354450.022.78217112-13
AACGCAG684500.022.5804316
TCAACGC678050.022.5636564
TATCAAC693850.022.1771892
TCACGCA11900.021.8834784
ATCACGC11800.021.6748453
GTACTAG22850.021.380771
ACGCAGA736050.020.7968337
CGCAGAG743600.020.5606638
TACTTTT417250.019.49255414-15
GGTATCA374400.019.4366381
GCAGAGT784750.019.388099
GAGTACT345150.019.3608412-13
AGAGTAC758950.017.60628510-11
TAGTACT31800.017.5480734
GTATAAG23800.017.3993591
Produced by FastQC (version 0.11.5)