##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765514_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7804532 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35986257728202 33.0 33.0 33.0 33.0 33.0 2 32.308564434100596 33.0 33.0 33.0 33.0 33.0 3 32.36260598329278 33.0 33.0 33.0 33.0 33.0 4 36.23254437293613 37.0 37.0 37.0 33.0 37.0 5 36.16260014053373 37.0 37.0 37.0 33.0 37.0 6 36.168425217553086 37.0 37.0 37.0 33.0 37.0 7 36.05590431303248 37.0 37.0 37.0 33.0 37.0 8 36.07180635558929 37.0 37.0 37.0 33.0 37.0 9 36.099602256740056 37.0 37.0 37.0 33.0 37.0 10-11 36.05472076993213 37.0 37.0 37.0 33.0 37.0 12-13 36.02595171625922 37.0 37.0 37.0 33.0 37.0 14-15 37.49713916222011 38.5 37.0 40.0 33.0 40.0 16-17 37.49691820086073 37.0 37.0 40.0 33.0 40.0 18-19 37.54761611586704 40.0 37.0 40.0 33.0 40.0 20-21 37.53261841965668 40.0 37.0 40.0 33.0 40.0 22-23 37.49934960866327 40.0 37.0 40.0 33.0 40.0 24-25 37.47376293671421 40.0 37.0 40.0 33.0 40.0 26-27 37.343098919960866 38.5 37.0 40.0 33.0 40.0 28-29 37.2341511316758 37.0 37.0 40.0 33.0 40.0 30-31 37.073817238496815 37.0 37.0 40.0 33.0 40.0 32-33 36.94868135590961 37.0 37.0 40.0 33.0 40.0 34-35 36.84559125390222 37.0 37.0 40.0 33.0 40.0 36-37 36.695141105193755 37.0 37.0 40.0 33.0 40.0 38-39 36.57617080691065 37.0 37.0 40.0 33.0 40.0 40-41 36.40502294051713 37.0 37.0 40.0 33.0 40.0 42-43 36.24201124423604 37.0 37.0 40.0 33.0 40.0 44-45 35.956846483555964 37.0 37.0 40.0 33.0 40.0 46-47 35.70950955162974 37.0 37.0 40.0 27.0 40.0 48-49 35.4365708283341 37.0 37.0 40.0 27.0 40.0 50-51 35.23384355397607 37.0 33.0 40.0 27.0 40.0 52-53 35.083301279307975 37.0 33.0 40.0 27.0 40.0 54-55 34.885351998044214 37.0 33.0 40.0 27.0 40.0 56-57 34.686114811240444 37.0 33.0 40.0 27.0 40.0 58-59 34.48568331835913 37.0 33.0 38.5 27.0 40.0 60-61 34.32293403371272 37.0 33.0 37.0 27.0 40.0 62-63 34.1111180016944 37.0 33.0 37.0 27.0 40.0 64-65 33.89544299389124 37.0 33.0 37.0 27.0 40.0 66-67 33.66921213213041 37.0 33.0 37.0 27.0 40.0 68-69 33.46576700563212 37.0 33.0 37.0 27.0 40.0 70-71 33.27469436988663 37.0 33.0 37.0 27.0 37.0 72-73 33.06542487108773 37.0 33.0 37.0 27.0 37.0 74-75 32.830964431948004 37.0 33.0 37.0 22.0 37.0 76-77 32.654655333593354 37.0 33.0 37.0 22.0 37.0 78-79 32.49234124480495 33.0 33.0 37.0 22.0 37.0 80-81 32.31649207152972 33.0 33.0 37.0 22.0 37.0 82-83 32.22427654854897 33.0 33.0 37.0 22.0 37.0 84-85 32.089646566892156 33.0 33.0 37.0 22.0 37.0 86-87 31.97172046959382 33.0 33.0 37.0 22.0 37.0 88-89 31.85223207490212 33.0 33.0 37.0 22.0 37.0 90-91 31.752376119413697 33.0 33.0 37.0 22.0 37.0 92-93 31.678391542247503 33.0 33.0 37.0 22.0 37.0 94-95 31.573361093272474 33.0 33.0 37.0 22.0 37.0 96-97 31.51220508801809 33.0 33.0 37.0 22.0 37.0 98-99 30.716338660665365 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.004409492329756404 1101 2 0.09901065266348041 1101 3 0.15578372320273814 1101 4 0.17056509639970585 1101 5 0.09552439687328018 1101 6 0.12170225266554979 1101 7 0.16364014018896 1101 8 0.11450640799294831 1101 9 0.11250353140061264 1101 10-11 0.11919912972435753 1101 12-13 0.13419641520121672 1101 14-15 0.18777737742068012 1101 16-17 0.20210297350399742 1101 18-19 0.1808908336116346 1101 20-21 0.19764426248779898 1101 22-23 0.19757305740350262 1101 24-25 0.25250504153609654 1101 26-27 0.27500473353470056 1101 28-29 0.23712743253052793 1101 30-31 0.17729188623442127 1101 32-33 0.2970760953590741 1101 34-35 0.3172461998375766 1101 36-37 0.2987269775460959 1101 38-39 0.26141952907222077 1101 40-41 0.2675249065067007 1101 42-43 0.30430519747134355 1101 44-45 0.34038829709218277 1101 46-47 0.3566565073811958 1101 48-49 0.35293886112420125 1101 50-51 0.44547663171680085 1101 52-53 0.4637203244887118 1101 54-55 0.32433517505290155 1101 56-57 0.42738525784668013 1101 58-59 0.39357167992205433 1101 60-61 0.2537330541733027 1101 62-63 0.3358632776235879 1101 64-65 0.3277939579597273 1101 66-67 0.4301218214481253 1101 68-69 0.4093518214440266 1101 70-71 0.3293254079850598 1101 72-73 0.3421207746316952 1101 74-75 0.36747331854439125 1101 76-77 0.4730852095271345 1101 78-79 0.44489384627204487 1101 80-81 0.4118872692767681 1101 82-83 0.33095047549191037 1101 84-85 0.29834681303007926 1101 86-87 0.4019806940262427 1101 88-89 0.3781818405547135 1101 90-91 0.409524279420598 1101 92-93 0.3470644069362727 1101 94-95 0.42156661842044585 1101 96-97 0.45383190368237436 1101 98-99 0.48801189335077666 1102 1 0.004115314721993002 1102 2 0.06712285100216064 1102 3 0.10729688019232242 1102 4 0.06324119997114508 1102 5 0.07089969543711305 1102 6 0.04811552060319002 1102 7 0.021191182735137204 1102 8 0.10644705161898571 1102 9 0.05515994897519505 1102 10-11 0.0626440772390211 1102 12-13 0.04275480143145671 1102 14-15 0.13599439676925584 1102 16-17 0.06601518267852668 1102 18-19 0.15256542918032778 1102 20-21 0.11695898224266443 1102 22-23 0.1477640678440082 1102 24-25 0.17894704890794344 1102 26-27 0.07586260932843913 1102 28-29 0.05416430612318379 1102 30-31 0.15548988408428244 1102 32-33 0.15887691729216158 1102 34-35 0.15866989749413563 1102 36-37 0.1460883844602776 1102 38-39 0.11380696734394036 1102 40-41 0.08219852139928463 1102 42-43 0.10631945249586039 1102 44-45 0.1330812974181086 1102 46-47 0.1890631780940666 1102 48-49 0.2119371352556243 1102 50-51 0.2511443265510849 1102 52-53 0.28747381818602236 1102 54-55 0.1090902876745048 1102 56-57 0.2007863826589329 1102 58-59 0.2600424905116796 1102 60-61 0.15203807275612036 1102 62-63 0.19705355598187424 1102 64-65 0.14879230698161194 1102 66-67 0.22846826326312453 1102 68-69 0.24260895697710794 1102 70-71 0.18390194486939748 1102 72-73 0.1360450998861964 1102 74-75 0.3016535327387544 1102 76-77 0.2777218532219976 1102 78-79 0.22408141899894218 1102 80-81 0.18737620866025395 1102 82-83 0.17935424297689195 1102 84-85 0.14692150723203667 1102 86-87 0.1664918176898027 1102 88-89 0.26197304345367556 1102 90-91 0.17821363218055097 1102 92-93 0.18186667741933604 1102 94-95 0.24110545788762394 1102 96-97 0.28480326724464433 1102 98-99 0.1375391149274563 1103 1 -0.019945621173917516 1103 2 0.06866813600905886 1103 3 0.07506163552194067 1103 4 0.059066416936445876 1103 5 0.005111271238483539 1103 6 0.09111038642907232 1103 7 0.03230122520128731 1103 8 0.14247868859946777 1103 9 0.04505338995676311 1103 10-11 0.0937733461300212 1103 12-13 0.10608562325779047 1103 14-15 0.17652124523970514 1103 16-17 0.15494818928777931 1103 18-19 0.1668537635869427 1103 20-21 0.1425298539105171 1103 22-23 0.14459045062729103 1103 24-25 0.17580003061824812 1103 26-27 0.17300819071640205 1103 28-29 0.13077870887602927 1103 30-31 0.16186477580505 1103 32-33 0.16044927120981356 1103 34-35 0.24750869209591286 1103 36-37 0.1543352034739982 1103 38-39 0.06839200815331026 1103 40-41 0.04235270806751856 1103 42-43 0.07549946519388584 1103 44-45 0.07818364853939386 1103 46-47 0.1267272723782824 1103 48-49 0.032850879218983664 1103 50-51 0.07609266923917346 1103 52-53 0.044181042224984424 1103 54-55 -0.004662929705006036 1103 56-57 0.03234309135606139 1103 58-59 0.09446690006357272 1103 60-61 0.04378816046011025 1103 62-63 0.033388248522534525 1103 64-65 0.06377521205858727 1103 66-67 0.12184492881014819 1103 68-69 0.1553022411979228 1103 70-71 -0.039944331873506655 1103 72-73 -0.001892870784580225 1103 74-75 0.06813185862425541 1103 76-77 0.10260870696888702 1103 78-79 0.08469411175720865 1103 80-81 0.03187487750844298 1103 82-83 -0.011522459236040561 1103 84-85 0.0014511394699781022 1103 86-87 -0.012222663271202805 1103 88-89 0.13527907137757111 1103 90-91 0.0628096848165498 1103 92-93 0.08066444035988596 1103 94-95 0.031134640218386522 1103 96-97 0.07762102812960592 1103 98-99 0.0894658021425272 1104 1 -0.013034952837045921 1104 2 0.011007869220570399 1104 3 -0.029560012565546856 1104 4 -0.014880385665534845 1104 5 -0.04074395048039037 1104 6 0.03602708636986307 1104 7 0.0429414711297369 1104 8 0.056509367719783654 1104 9 0.006750311767973471 1104 10-11 -0.04059864683215153 1104 12-13 -0.08377356287571303 1104 14-15 -0.003482344905414436 1104 16-17 -0.013280626325482103 1104 18-19 -0.012773923596128611 1104 20-21 -0.001989177876055237 1104 22-23 0.010217950118025954 1104 24-25 0.04797715374637335 1104 26-27 -0.01765600800369782 1104 28-29 -0.0354088952854994 1104 30-31 -0.04752688853881182 1104 32-33 0.03492790596473583 1104 34-35 0.015639518236184813 1104 36-37 0.1408679023458248 1104 38-39 0.007201651227894956 1104 40-41 -0.002992543196022268 1104 42-43 0.04212989847840731 1104 44-45 0.04682050485386213 1104 46-47 -0.0022250183090122277 1104 48-49 0.04285897523854487 1104 50-51 -0.14025335180890153 1104 52-53 -0.00865105413267031 1104 54-55 -0.08908348849062975 1104 56-57 0.035954896575560724 1104 58-59 -0.0324069387973438 1104 60-61 -0.014260319094638874 1104 62-63 -0.03191428357417436 1104 64-65 9.208369568654007E-4 1104 66-67 0.04263748990823757 1104 68-69 -0.01366918372896464 1104 70-71 -0.13480822618544863 1104 72-73 -0.049479174807750326 1104 74-75 -0.035448463663300345 1104 76-77 -0.009350192067387297 1104 78-79 0.005556131747759707 1104 80-81 0.016213857748823557 1104 82-83 0.03189074966714145 1104 84-85 0.09678769595136316 1104 86-87 0.058416737269638475 1104 88-89 -0.009576353331549825 1104 90-91 0.009743387642263457 1104 92-93 0.06523806105275654 1104 94-95 -0.01896945483171919 1104 96-97 -0.010625710186889847 1104 98-99 0.10162572986095952 1105 1 -0.014367334384175479 1105 2 0.07368023107392219 1105 3 0.046083246228448616 1105 4 0.038395588836444006 1105 5 0.06410726284339319 1105 6 0.06546363448160974 1105 7 0.026057086525554496 1105 8 0.0475823992504516 1105 9 0.06352507739458702 1105 10-11 0.04680610667267615 1105 12-13 0.02109002456104747 1105 14-15 0.06484132347200244 1105 16-17 0.06204223692294164 1105 18-19 0.07192452311521436 1105 20-21 0.07326506529130228 1105 22-23 0.07978289937362604 1105 24-25 0.08542823968941349 1105 26-27 0.05268739257000732 1105 28-29 0.07346790605214437 1105 30-31 0.07969435017796656 1105 32-33 0.09696623336286336 1105 34-35 0.1373219459249455 1105 36-37 0.12928522353519156 1105 38-39 0.10359171237452358 1105 40-41 0.07273075574648402 1105 42-43 0.11156768556779895 1105 44-45 0.16927773855241668 1105 46-47 0.040182889526661825 1105 48-49 0.07077091942728231 1105 50-51 0.07601321782611592 1105 52-53 -0.04391090751494886 1105 54-55 0.06439607363265765 1105 56-57 0.06930757377541852 1105 58-59 0.08106187237192586 1105 60-61 0.034028414413619146 1105 62-63 0.027771210901072152 1105 64-65 0.08171115005125529 1105 66-67 0.14918405282677583 1105 68-69 0.1702671393704307 1105 70-71 0.0967850992037711 1105 72-73 0.10213170758241574 1105 74-75 0.1567475656105799 1105 76-77 0.05294580594227227 1105 78-79 0.09555797519156783 1105 80-81 0.05660333630113712 1105 82-83 0.08331049686614733 1105 84-85 0.03207732552196774 1105 86-87 0.09377412400848328 1105 88-89 0.12758998775403185 1105 90-91 0.10198447207250183 1105 92-93 0.15148911007565502 1105 94-95 0.14510281434536765 1105 96-97 0.01755058293212386 1105 98-99 0.13290154960821 1106 1 -0.030420573805230333 1106 2 0.04608261385880752 1106 3 -0.0038970133178324318 1106 4 -0.0015498423268596184 1106 5 0.043926152864479207 1106 6 0.04342645616326024 1106 7 0.0014404384886717025 1106 8 0.03383523986207848 1106 9 -0.014965266592341209 1106 10-11 -0.0061031188807305625 1106 12-13 0.027714741355268302 1106 14-15 0.051902926867121835 1106 16-17 0.026109333103555343 1106 18-19 -0.013494516431819648 1106 20-21 0.023414546473787823 1106 22-23 0.01549349501522812 1106 24-25 0.04340338611567773 1106 26-27 0.007548778856559579 1106 28-29 -0.012924584553921648 1106 30-31 -0.011293296920904083 1106 32-33 -0.0696099592606032 1106 34-35 0.024762980224643627 1106 36-37 0.048219615611351685 1106 38-39 -0.01958142118686368 1106 40-41 -0.001754466125881038 1106 42-43 -0.011131162832981545 1106 44-45 -0.012672074577274373 1106 46-47 0.030170526680166176 1106 48-49 -0.041527496019504895 1106 50-51 -0.058717691650500115 1106 52-53 -0.09175932853578672 1106 54-55 -0.12360347527991422 1106 56-57 0.009802110676773168 1106 58-59 0.060387953748652023 1106 60-61 -0.04441227220119259 1106 62-63 -0.05589811244772136 1106 64-65 -0.0902271920519766 1106 66-67 -0.046210002781165826 1106 68-69 -0.06594446063750325 1106 70-71 -0.1369953994016342 1106 72-73 -0.056809034252367496 1106 74-75 -0.07057953933956895 1106 76-77 0.06313241376611956 1106 78-79 -0.0396695774085174 1106 80-81 0.012025805781064491 1106 82-83 -0.14153756354708236 1106 84-85 -0.08215686684827261 1106 86-87 -0.012883141093738004 1106 88-89 0.0014152794605486463 1106 90-91 -0.14599389510801686 1106 92-93 -0.0553960188614262 1106 94-95 -0.012052493378455154 1106 96-97 -0.03628080535329303 1106 98-99 0.028765466671803352 1107 1 -0.011128237502703087 1107 2 0.0355844072261533 1107 3 0.023825676006609342 1107 4 -1.0567740429934247E-4 1107 5 0.046898468145997185 1107 6 0.05728706618989321 1107 7 0.04913071271175795 1107 8 0.0685729001051385 1107 9 0.05952295663379914 1107 10-11 -0.015288392913319626 1107 12-13 -0.05589421969037289 1107 14-15 0.006535709179296134 1107 16-17 -0.02576304887271874 1107 18-19 -0.06094088825385313 1107 20-21 -0.0219363568872879 1107 22-23 0.05756402703283925 1107 24-25 0.030327143460795014 1107 26-27 0.07531592263936915 1107 28-29 -0.010484267314041062 1107 30-31 -0.0015448180685169177 1107 32-33 0.03884212949559185 1107 34-35 0.013454443453731812 1107 36-37 0.02711667680019758 1107 38-39 -0.03974674154861901 1107 40-41 -0.11145085152852374 1107 42-43 -0.045276249759766074 1107 44-45 -0.10093539218496517 1107 46-47 0.01739667289839275 1107 48-49 -0.11705510334395797 1107 50-51 -0.11445057010649862 1107 52-53 -0.14483947511867967 1107 54-55 -0.12097163143253908 1107 56-57 -0.14087740388389136 1107 58-59 -0.13230378917008778 1107 60-61 -0.12834224191598764 1107 62-63 -0.2051959399650798 1107 64-65 -0.1246774743629544 1107 66-67 -0.08924053762844864 1107 68-69 -0.18827041671510614 1107 70-71 -0.15453167227082787 1107 72-73 -0.16608063439897336 1107 74-75 -0.13357626767890451 1107 76-77 -0.10010726364070166 1107 78-79 -0.11222308872186915 1107 80-81 -0.13389239672368092 1107 82-83 -0.14355595282328437 1107 84-85 -0.07685717523882118 1107 86-87 -0.07386503823062895 1107 88-89 -0.09542897453776078 1107 90-91 -0.14622775974628155 1107 92-93 -0.04748205372122172 1107 94-95 -0.10645456477185533 1107 96-97 -0.1385338561950249 1107 98-99 -0.13173259134101656 1108 1 -0.0971541872888011 1108 2 -0.025642691912310056 1108 3 0.01598486936802601 1108 4 -0.06769751095876586 1108 5 -0.019204982600065534 1108 6 -0.021187271397771212 1108 7 -0.09087108480877504 1108 8 -0.060884311896089116 1108 9 -0.09066205787515713 1108 10-11 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66-67 -0.13790763877985768 2215 68-69 -0.02495204701244802 2215 70-71 -0.18794745701182336 2215 72-73 -0.1995789431912982 2215 74-75 -0.29520838984402786 2215 76-77 -0.1472258358000076 2215 78-79 -0.1127342377325391 2215 80-81 -0.1134667681268553 2215 82-83 -0.15536659198371439 2215 84-85 -0.07013311528425703 2215 86-87 -0.1014313623861618 2215 88-89 -0.047464562345201244 2215 90-91 -0.16877927076531662 2215 92-93 -0.021405590394437013 2215 94-95 -0.04555989282240702 2215 96-97 -0.042136902129918496 2215 98-99 -0.11991705188201607 2216 1 -0.026378211145122066 2216 2 -0.067560004815725 2216 3 -0.08519305289244272 2216 4 -0.17052326001713425 2216 5 -0.12547519584578737 2216 6 -0.22697073351052666 2216 7 -0.11613049486385307 2216 8 -0.2143692833946389 2216 9 -0.10497880720371455 2216 10-11 -0.10676859883932366 2216 12-13 -0.09248691592708269 2216 14-15 -0.25702066038589066 2216 16-17 -0.2189890827723957 2216 18-19 -0.1406798580340478 2216 20-21 -0.18869196023222656 2216 22-23 -0.26380103453990245 2216 24-25 -0.3062273473917543 2216 26-27 -0.3019086285813586 2216 28-29 -0.19530900731543 2216 30-31 -0.2224174269805843 2216 32-33 -0.17002298175895447 2216 34-35 -0.2051032900156784 2216 36-37 -0.18848018585621418 2216 38-39 -0.19645554460182524 2216 40-41 -0.14126474626880992 2216 42-43 -0.1860410835317552 2216 44-45 -0.21548802090045882 2216 46-47 -0.19187994459181112 2216 48-49 -0.10032192620163016 2216 50-51 -0.09716634904285826 2216 52-53 -0.11937165489251811 2216 54-55 -0.018765463679194738 2216 56-57 -0.16300945723244098 2216 58-59 -0.1917457312267814 2216 60-61 -0.22074470702801108 2216 62-63 -0.09141377640563775 2216 64-65 -0.18956878972360158 2216 66-67 -0.16757822268837685 2216 68-69 -0.07244724061605723 2216 70-71 -0.19734684167691796 2216 72-73 -0.1672388244662102 2216 74-75 -0.12234144725425011 2216 76-77 -0.10761639218932118 2216 78-79 0.02936075899776114 2216 80-81 -0.11221681351906909 2216 82-83 -0.06152427033210017 2216 84-85 -0.1328481485746167 2216 86-87 -0.13166641659667633 2216 88-89 -0.09332557723558565 2216 90-91 -0.10147279979797119 2216 92-93 0.06939122341789172 2216 94-95 -0.049132535658610976 2216 96-97 -0.0833952368209836 2216 98-99 -0.18471486444681773 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 22.0 10 77.0 11 201.0 12 450.0 13 745.0 14 1673.0 15 4157.0 16 9220.0 17 17190.0 18 27018.0 19 37701.0 20 48544.0 21 61455.0 22 74811.0 23 73460.0 24 62304.0 25 59743.0 26 69425.0 27 88137.0 28 114585.0 29 150430.0 30 196576.0 31 255445.0 32 335062.0 33 438446.0 34 585465.0 35 841144.0 36 1409249.0 37 2089883.0 38 751065.0 39 847.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98264012194606 18.73371990610461 13.20175073107381 25.081889240875523 2 16.166798982949906 21.231740737304943 37.000847712585454 25.600612567159697 3 18.113347475543698 26.181992719102183 29.679601544333472 26.02505826102065 4 13.164517744305487 17.32808578400345 36.16001574469808 33.34738072699298 5 14.191311768655531 37.00149408261277 33.15259119541986 15.65460295331184 6 31.61522048983847 36.847295904482166 17.21618926029133 14.321294345388038 7 27.172020051939054 31.91372653735035 22.314688439998708 18.59956497071189 8 27.45506072625495 31.987606688011528 20.20303075187596 20.35430183385756 9 27.40289872602226 14.02793915125212 19.880461762473395 38.68870036025222 10-11 25.658277780141077 24.84766543336615 27.096185908392712 22.397870878100058 12-13 26.861886145126963 22.95341988475414 27.1601935900833 23.0245003800356 14-15 24.38926212764349 23.566172172031727 25.458250808114492 26.586314892210293 16-17 23.3954896773383 25.90794953130473 26.26988449564332 24.426676295713655 18-19 23.80455962618894 24.50832871258838 28.94979836894911 22.73731329227357 20-21 25.02635492503477 23.86303524041874 28.066516851744282 23.044092982802205 22-23 24.115654852847037 23.709352463414845 28.05648692323896 24.118505760499158 24-25 23.629443764212894 24.36966111484968 28.135831847444535 23.86506327349289 26-27 24.020902214251922 24.143574528235646 28.04958708606743 23.785936171445 28-29 23.590319060771357 23.95232026724985 28.713105411061164 23.744255260917633 30-31 24.07652469795361 23.981573225645214 28.268637645936394 23.673264430464783 32-33 23.67343102699816 24.033888258770673 27.842041008993235 24.450639705237933 34-35 24.181988106397668 24.01946715062479 28.307168194069803 23.491376548907734 36-37 24.18868293447961 24.10394370860418 27.77779628554281 23.9295770713734 38-39 24.31585263536622 23.66511534580164 27.808662966594284 24.210369052237855 40-41 24.073717680957678 24.16920066443446 27.8913905407781 23.865691113829758 42-43 24.744545861302125 23.900215925823613 27.41789001569857 23.93734819717569 44-45 24.242241559135127 24.2374943174043 26.99564176301667 24.524622360443907 46-47 24.078285539735116 24.274966135061014 27.155561665965365 24.491186659238505 48-49 24.808540729924612 24.66382993880991 26.200962466423356 24.32666686484212 50-51 24.101861585038026 25.094176050530642 25.87876505599567 24.925197308435664 52-53 24.117301332097814 24.8304126371703 25.801502255356247 25.25078377537564 54-55 24.442746855288696 24.824531438912672 25.847712585456755 24.885009120341874 56-57 24.461306584430687 24.47379291929356 25.562000386442136 25.50290010983362 58-59 24.139325229108913 24.414992035715667 26.160125839728604 25.28555689544682 60-61 24.186152353529973 24.64765984686846 26.043771746979832 25.12241605262173 62-63 23.684431045961503 24.702711193957562 25.935731956765633 25.677125803315302 64-65 23.799575682436817 24.394339084009136 26.050306411710526 25.75577882184351 66-67 24.318850893301484 24.510893157975392 25.865458684774435 25.304797263948693 68-69 24.384203947142506 24.128845906455375 25.841664817313838 25.64528532908828 70-71 23.698173061498114 24.257847876080206 26.10567167896807 25.938307383453612 72-73 24.214450014427516 24.369814871666872 25.979584682335854 25.43615043156976 74-75 24.479628871759797 24.273331910227476 25.90751187195737 25.339527346055352 76-77 24.079930604316473 24.522404965327784 25.952217432974738 25.445446997381005 78-79 24.09053899682447 24.567518606029463 25.906606890245037 25.435335506901026 80-81 23.65257066154417 24.54755111391746 26.121534832087217 25.678343392451154 82-83 23.851029613438655 24.692688132060724 25.55845444140077 25.89782781309985 84-85 23.816216833323693 24.772882675466203 25.311789612699798 26.099110878510306 86-87 23.656824746526766 24.46359927185346 25.581861837436044 26.297714144183725 88-89 23.6348440023981 24.238815218531947 25.7613193190451 26.365021460024852 90-91 23.86463120594865 24.360219784655115 25.55895140693636 26.21619760245988 92-93 23.52603584664215 24.64944512803205 25.425160098523747 26.39935892680205 94-95 23.081626999563838 24.645843167463905 25.881093252927943 26.391436580044314 96-97 23.677882929013787 24.529188303856785 25.474377007489718 26.318551759639714 98-99 23.37258107760508 24.515648539156825 25.55831216227341 26.553458220964682 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 284.0 1 264.0 2 700.5 3 1134.0 4 767.5 5 645.5 6 1347.5 7 3606.5 8 6514.0 9 6291.5 10 3643.5 11 2121.5 12 2034.5 13 2284.5 14 2632.0 15 3217.0 16 3885.0 17 4668.5 18 5577.0 19 6700.5 20 8193.0 21 10208.0 22 12553.0 23 15299.5 24 18979.5 25 23727.5 26 29394.0 27 35116.0 28 41942.0 29 50565.0 30 58089.5 31 66900.5 32 77760.0 33 88086.0 34 96503.5 35 104375.0 36 111223.5 37 121402.5 38 134340.0 39 138192.0 40 142468.5 41 152367.0 42 165443.0 43 188090.0 44 212574.0 45 237070.5 46 276914.5 47 311869.5 48 333228.0 49 356824.0 50 385017.0 51 376140.5 52 348391.5 53 342380.0 54 328939.0 55 319320.0 56 302825.5 57 286653.5 58 272814.0 59 241026.5 60 198705.5 61 150931.5 62 121912.0 63 96600.0 64 71457.5 65 57386.5 66 42346.0 67 31415.0 68 24658.5 69 20701.5 70 18786.0 71 17344.0 72 18176.5 73 15885.0 74 10449.5 75 7948.0 76 6252.0 77 4258.5 78 2297.5 79 1512.5 80 1219.5 81 894.5 82 667.5 83 523.5 84 324.5 85 233.0 86 124.0 87 47.5 88 25.0 89 21.5 90 20.5 91 12.0 92 3.5 93 3.0 94 3.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05589060304961271 2 0.0 3 0.0 4 0.0 5 0.0052789840569556256 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012749002758909822 16-17 0.00470880252653202 18-19 6.983122114176737E-4 20-21 1.7297641934199256E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.4344829388873028E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.9219602149110284E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 7.687840859644114E-5 76-77 1.5375681719288227E-4 78-79 7.047187454673772E-5 80-81 5.1252272397627434E-5 82-83 1.2813068099406858E-5 84-85 3.907985770319091E-4 86-87 7.559710178650046E-4 88-89 2.69074430087544E-4 90-91 9.545735734058108E-4 92-93 0.001524755103829416 94-95 0.0010250454479525486 96-97 0.0021077497023524283 98-99 6.342468709206394E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7804532.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.55446295286816 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48078927632343 29.064931571778473 2 14.754039471043514 11.671762153250906 3 4.8259302673431455 5.726612399182487 4 2.193462301010262 3.4704489329529347 5 1.1848441521938777 2.343293706143761 6 0.6977893963160784 1.6560410895293138 7 0.4772629858207953 1.321451676399672 8 0.33777791611504643 1.068849925541569 9 0.25447123008416056 0.9058925558641246 >10 1.4907038086050972 11.155472283394356 >50 0.14356158530041907 3.9406303964080265 >100 0.12814793249118112 10.500495738118484 >500 0.01721474790092448 4.727411472576145 >1k 0.013291715452968473 9.92478910374197 >5k 5.5111990852587E-4 1.3280873706429874 >10k+ 1.6209409074290293E-4 1.1938296244748685 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25471 0.3263616575599921 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 19189 0.2458699637595182 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 18509 0.23715707745192152 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16227 0.20791765604907508 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13704 0.17559028523427156 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2813068099406857E-5 0.0 0.0 0.0 0.0 2 1.2813068099406857E-5 0.0 0.0 0.0 0.0 3 2.5626136198813714E-5 0.0 0.0 1.2813068099406857E-5 0.0 4 2.5626136198813714E-5 0.0 0.0 2.5626136198813714E-5 0.0 5 6.406534049703429E-5 0.0 0.0 3.8439204298220575E-5 0.0 6 6.406534049703429E-5 0.0 0.0 5.125227239762743E-5 5.125227239762743E-5 7 6.406534049703429E-5 0.0 0.0 5.125227239762743E-5 5.125227239762743E-5 8 6.406534049703429E-5 0.0 0.0 5.125227239762743E-5 5.125227239762743E-5 9 6.406534049703429E-5 0.0 0.0 1.1531761289466172E-4 1.0250454479525485E-4 10-11 8.9691476695848E-5 0.0 0.0 1.7297641934199259E-4 1.1531761289466172E-4 12-13 8.9691476695848E-5 1.2813068099406857E-5 0.0 1.986025555408063E-4 1.2172414694436515E-4 14-15 8.9691476695848E-5 1.2813068099406857E-5 0.0 2.3063522578932344E-4 2.2422869173962E-4 16-17 8.9691476695848E-5 1.2813068099406857E-5 0.0 2.6266789603784056E-4 2.69074430087544E-4 18-19 8.9691476695848E-5 1.2813068099406857E-5 0.0 2.754809641372474E-4 2.882940322366543E-4 20-21 8.9691476695848E-5 1.2813068099406857E-5 0.0 5.765880644733086E-4 3.3313977058457833E-4 22-23 9.609801074555142E-5 1.2813068099406857E-5 0.0 0.001883521010612808 3.7157897488279885E-4 24-25 1.0250454479525485E-4 1.2813068099406857E-5 0.0 0.004958657354470454 3.8439204298220574E-4 26-27 1.2813068099406857E-4 1.2813068099406857E-5 0.0 0.008629601364950518 3.8439204298220574E-4 28-29 1.2813068099406857E-4 1.2813068099406857E-5 0.0 0.02207050980122831 4.100181791810194E-4 30-31 1.3453721504377202E-4 1.2813068099406857E-5 0.0 0.06028548540770927 4.228312472804263E-4 32-33 1.4094374909347544E-4 1.2813068099406857E-5 0.0 0.12249933756437927 4.740835196780537E-4 34-35 1.4735028314317886E-4 1.9219602149110284E-5 0.0 0.20292696602435611 5.253357920756811E-4 36-37 1.537568171928823E-4 3.203267024851714E-5 0.0 0.3245678280260751 5.445553942247914E-4 38-39 1.537568171928823E-4 3.8439204298220575E-5 0.0 0.5033165345468504 5.829945985230121E-4 40-41 1.6656988529228914E-4 3.8439204298220575E-5 0.0 0.7146040275060696 6.022142006721223E-4 42-43 1.8578948744139942E-4 3.8439204298220575E-5 0.0 0.9100033160220241 6.214338028212327E-4 44-45 1.9219602149110287E-4 3.8439204298220575E-5 0.0 1.13583364127407 6.406534049703429E-4 46-47 1.9219602149110287E-4 4.4845738347924E-5 0.0 1.3856051842698576 6.406534049703429E-4 48-49 1.986025555408063E-4 5.125227239762743E-5 0.0 1.6416743502365034 6.598730071194531E-4 50-51 2.050090895905097E-4 5.125227239762743E-5 0.0 1.9216847339468912 6.662795411691566E-4 52-53 2.050090895905097E-4 5.125227239762743E-5 0.0 2.206576896603153 6.7268607521886E-4 54-55 2.050090895905097E-4 7.047187454673772E-5 0.0 2.4851906558907055 6.854991433182669E-4 56-57 2.050090895905097E-4 7.687840859644115E-5 0.0 2.8013851439138184 7.047187454673772E-4 58-59 2.1782215768991657E-4 7.687840859644115E-5 0.0 3.146460287432994 7.431579497655977E-4 60-61 2.1782215768991657E-4 7.687840859644115E-5 0.0 3.4907922730024046 7.431579497655977E-4 62-63 2.1782215768991657E-4 8.328494264614458E-5 0.0 3.840332770754223 7.62377551914708E-4 64-65 2.1782215768991657E-4 8.9691476695848E-5 0.0 4.205601309598064 7.944102221632252E-4 66-67 2.1782215768991657E-4 8.9691476695848E-5 0.0 4.601422609324941 8.008167562129286E-4 68-69 2.3063522578932344E-4 8.9691476695848E-5 0.0 5.0306155449167225 8.136298243123354E-4 70-71 2.3704175983902689E-4 8.9691476695848E-5 0.0 5.50590349299612 8.392559605111493E-4 72-73 2.498548279384337E-4 8.9691476695848E-5 0.0 5.965245577825807 8.776951648093697E-4 74-75 2.5626136198813714E-4 8.9691476695848E-5 0.0 6.422620856702234 8.841016988590731E-4 76-77 2.5626136198813714E-4 8.9691476695848E-5 0.0 6.9140532705868845 8.9691476695848E-4 78-79 2.818874981869509E-4 8.9691476695848E-5 0.0 7.420233525853953 9.225409031572938E-4 80-81 3.011071003360612E-4 8.9691476695848E-5 0.0 7.971733602988623 9.289474372069972E-4 82-83 3.13920168435468E-4 8.9691476695848E-5 0.0 8.552754989024326 9.737931755549211E-4 84-85 3.2032670248517144E-4 8.9691476695848E-5 0.0 9.123103089333224 9.930127777040315E-4 86-87 3.2032670248517144E-4 8.9691476695848E-5 0.0 9.707122733304189 9.99419311753735E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 53630 0.0 28.691063 1 TATCACG 900 0.0 28.418129 2 ATCAACG 66045 0.0 23.186068 3 CAACGCA 66120 0.0 23.1457 5 AGTACTT 35445 0.0 22.782171 12-13 AACGCAG 68450 0.0 22.580431 6 TCAACGC 67805 0.0 22.563656 4 TATCAAC 69385 0.0 22.177189 2 TCACGCA 1190 0.0 21.883478 4 ATCACGC 1180 0.0 21.674845 3 GTACTAG 2285 0.0 21.38077 1 ACGCAGA 73605 0.0 20.796833 7 CGCAGAG 74360 0.0 20.560663 8 TACTTTT 41725 0.0 19.492554 14-15 GGTATCA 37440 0.0 19.436638 1 GCAGAGT 78475 0.0 19.38809 9 GAGTACT 34515 0.0 19.36084 12-13 AGAGTAC 75895 0.0 17.606285 10-11 TAGTACT 3180 0.0 17.548073 4 GTATAAG 2380 0.0 17.399359 1 >>END_MODULE