FastQCFastQC Report
Fri 27 May 2016
ERR765511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765511_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12447806
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT772830.6208563983082641No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA760680.6110956420753987No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG306150.24594695643553569No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC219110.17602298750478598No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC214700.17248019450174593No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201250.161675077519685No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG197120.15835722375493322No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG191800.15408337822745632No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT165100.13263381514782605No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC145230.11667116277358436No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG128100.10290970151687776No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT52700.021.848764
GTATAAG57150.020.9749891
GTACTAG44100.020.8927351
CTAGTAC63150.018.3081863
CATGGGG314850.018.2405874
ATAAGGT46200.017.9041713
TTTAGGG59950.017.405262
GTGATCG36800.017.363418
CTAACGC21400.017.3498953
TAGGCAT66000.017.1588675
ATTTAGG57150.017.109011
CTGTGCG69800.016.9619929
CTATTGA52200.016.920739
GTATTAT31000.016.832131
GGGCTAT60950.016.806846
TCTAACG22700.016.5645962
GTCCTAC42600.016.5523431
GTACAAG58850.016.4550271
TACGGGC31350.016.3403114
GTACATA33350.016.209881
Produced by FastQC (version 0.11.5)