FastQCFastQC Report
Fri 27 May 2016
ERR765510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765510_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9167416
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA646060.7047351183801411No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT534970.5835559333186146No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG258960.28247872682989406No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG241830.26379298157736053No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT225190.24564173808628298No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG203570.22205821138693826No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA191080.20843387056941673No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC187320.20433238766518286No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC185080.20188895104138396No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC171290.186846544326122No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC141830.15471098944348113No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT121530.13256734504030362No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG96150.10488233543672504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA29050.023.1326051
ATGGGTA55250.018.3707835
GTATTAG46950.018.216731
CATGGGT77000.017.819814
TGATCGC17000.017.4115039
TATCACG6750.017.4044932
GTACAAG46100.017.2274271
GTATAAG30900.016.8810391
GTATAAT41000.016.734151
CATGGGG217550.016.567134
GTACTAG32750.016.5014151
ATTGCGT12100.016.3082946
ATGGGAG91150.016.2905855
GTGATCG17900.016.2735868
GGTCGCC50400.016.12777394
GTACAAA49400.016.0766091
GTATATA29200.015.9326121
TAATACG24000.015.8593264
AATACGG21500.015.5176545
TAACGCA5500.015.378744
Produced by FastQC (version 0.11.5)