##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765510_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9167416
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.675953725673626	33.0	33.0	33.0	27.0	33.0
2	31.599307809310716	33.0	33.0	33.0	27.0	33.0
3	31.63122072784741	33.0	33.0	33.0	27.0	33.0
4	35.35121052649951	37.0	37.0	37.0	33.0	37.0
5	35.4245492950249	37.0	37.0	37.0	33.0	37.0
6	35.4070821047065	37.0	37.0	37.0	33.0	37.0
7	35.334750490214475	37.0	37.0	37.0	33.0	37.0
8	35.31963587122042	37.0	37.0	37.0	33.0	37.0
9	35.31682188306934	37.0	37.0	37.0	33.0	37.0
10-11	35.283161252854676	37.0	37.0	37.0	33.0	37.0
12-13	35.246393313012085	37.0	37.0	37.0	33.0	37.0
14-15	36.74290748887145	37.0	37.0	40.0	33.0	40.0
16-17	36.747724440562095	37.0	37.0	40.0	33.0	40.0
18-19	36.71422748787663	38.5	37.0	40.0	33.0	40.0
20-21	36.80234179402353	40.0	37.0	40.0	33.0	40.0
22-23	36.79995366196974	37.0	37.0	40.0	33.0	40.0
24-25	36.792930690611186	37.0	37.0	40.0	33.0	40.0
26-27	36.74960157802373	37.0	37.0	40.0	33.0	40.0
28-29	36.68971436444032	37.0	37.0	40.0	33.0	40.0
30-31	36.6029329856963	37.0	37.0	40.0	33.0	40.0
32-33	36.47515434011067	37.0	37.0	40.0	33.0	40.0
34-35	36.39171332467077	37.0	37.0	40.0	33.0	40.0
36-37	36.323880960567294	37.0	37.0	40.0	33.0	40.0
38-39	36.21702391382698	37.0	37.0	40.0	33.0	40.0
40-41	36.119151678073735	37.0	37.0	40.0	30.0	40.0
42-43	35.97783230301756	37.0	37.0	40.0	27.0	40.0
44-45	35.82400482316936	37.0	37.0	40.0	27.0	40.0
46-47	35.64879040069742	37.0	37.0	40.0	27.0	40.0
48-49	35.425965233823796	37.0	33.0	40.0	27.0	40.0
50-51	35.345881216691815	37.0	33.0	40.0	27.0	40.0
52-53	35.21205495638029	37.0	33.0	40.0	27.0	40.0
54-55	35.047543113566576	37.0	33.0	40.0	27.0	40.0
56-57	34.886999891790666	37.0	33.0	40.0	27.0	40.0
58-59	34.72553885413294	37.0	33.0	40.0	27.0	40.0
60-61	34.51815909739451	37.0	33.0	40.0	27.0	40.0
62-63	34.27865322136576	37.0	33.0	37.0	27.0	40.0
64-65	34.102178411015714	37.0	33.0	37.0	27.0	40.0
66-67	33.91206622455008	37.0	33.0	37.0	27.0	40.0
68-69	33.70533103330317	37.0	33.0	37.0	27.0	40.0
70-71	33.46529261898882	37.0	33.0	37.0	27.0	40.0
72-73	33.22001488751029	37.0	33.0	37.0	27.0	37.0
74-75	32.98853946411944	37.0	33.0	37.0	24.5	37.0
76-77	32.78346679151464	37.0	33.0	37.0	22.0	37.0
78-79	32.62428365855766	37.0	33.0	37.0	22.0	37.0
80-81	32.458381674836176	33.0	33.0	37.0	22.0	37.0
82-83	32.33898439865716	33.0	33.0	37.0	22.0	37.0
84-85	32.18378864884063	33.0	33.0	37.0	22.0	37.0
86-87	32.05165784993285	33.0	33.0	37.0	22.0	37.0
88-89	31.92679469329198	33.0	33.0	37.0	22.0	37.0
90-91	31.805510135026054	33.0	33.0	37.0	22.0	37.0
92-93	31.695173427277652	33.0	33.0	37.0	22.0	37.0
94-95	31.594742291611944	33.0	33.0	37.0	22.0	37.0
96-97	31.525501897154008	33.0	33.0	37.0	22.0	37.0
98-99	31.414321658360436	33.0	33.0	37.0	22.0	37.0
100	31.346130359961847	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	4.0
6	8.0
7	143.0
8	724.0
9	1406.0
10	2450.0
11	4847.0
12	9700.0
13	17435.0
14	23846.0
15	26470.0
16	30111.0
17	34902.0
18	41750.0
19	48539.0
20	55658.0
21	65210.0
22	74751.0
23	77225.0
24	79213.0
25	86625.0
26	99187.0
27	116240.0
28	141748.0
29	178457.0
30	225510.0
31	290527.0
32	378046.0
33	499326.0
34	656568.0
35	904376.0
36	1450166.0
37	2357870.0
38	1186856.0
39	1522.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.097298633660564	19.275495550892256	12.962670001433613	24.664535814013572
2	15.372130544867844	21.743256378172994	37.763442244357634	25.121170832601525
3	18.048219289024683	27.278289843727162	29.420632395993014	25.252858471255145
4	12.68526756592006	17.9674031189225	37.35234556254535	31.994983752612082
5	13.415727629540413	38.24613126016869	33.78593227582612	14.552208834464775
6	30.435926546804463	37.33994399294196	18.2626816542415	13.961447806012076
7	26.42021481298547	32.420422505098486	22.991778708416856	18.167583973499184
8	26.455077417671458	33.084982725775724	21.130043624070293	19.329896232482525
9	27.247438100332744	15.104736165567264	20.05329527971677	37.59453045438322
10-11	24.69598848792288	26.30122272186623	27.510183894785616	21.492604895425277
12-13	25.93891779319276	24.090125287212885	27.156316458203705	22.81464046139065
14-15	24.171407733651446	24.676506444127767	24.976214671615207	26.17587115060558
16-17	23.39963126867389	26.984799745663413	26.380853983409324	23.23471500225337
18-19	23.937178628843668	26.320485532076777	27.756083127324	21.986252711755554
20-21	24.83160906337464	25.812562354388252	26.603640874502776	22.752187707734333
22-23	23.572662701373428	25.977086940151505	25.96426370783626	24.485986650638807
24-25	23.488719570215732	26.524188510450035	25.99196540841653	23.995126510917704
26-27	23.9892770630044	25.852913171275127	25.909295424735856	24.248514340984617
28-29	23.603389990285134	25.734217490771748	26.83506092957203	23.82733158937108
30-31	24.494263968985962	25.60168522760965	26.491475804311314	23.41257499909307
32-33	23.03392520015121	26.0626901347833	26.44933366571407	24.45405099935142
34-35	23.39605284790686	26.107828442313803	26.923732344419737	23.572386365359602
36-37	24.198703402434003	25.794199829284814	26.292948267013916	23.71414850126727
38-39	24.37327159702812	25.37143496813326	26.19317460543465	24.06211882940398
40-41	23.77591146080549	26.00763003277621	26.41234606213011	23.80411244428819
42-43	24.81729343401935	25.50797010360832	26.05769223950346	23.61704422286887
44-45	24.420779500474126	25.656177511009233	26.035302961875306	23.887740026641335
46-47	23.684161430304286	25.592370654375646	26.932659037801095	23.79080887751898
48-49	24.302186920371025	25.942154913491805	26.397525810851214	23.35813235528596
50-51	23.30929865421579	26.374642078957926	26.038149662078464	24.277909604747823
52-53	23.224326216100764	26.37481594069512	25.48120017305257	24.91965767015154
54-55	23.721753436298023	26.109231039238303	25.47738613690266	24.69162938756102
56-57	23.959683519094167	25.32214357196026	25.48565230629956	25.23252060264601
58-59	23.632261075679033	25.28310931808725	26.386531417448207	24.698098188785515
60-61	23.77663549989671	25.65184037157265	25.96563736372778	24.60588676480287
62-63	22.754250038677878	25.82573177562772	26.138498879378048	25.28151930631635
64-65	22.744431044621955	25.629563316397704	26.27530942307773	25.350696215902612
66-67	23.669138488192207	25.587111252697476	25.803983862063852	24.93976639704647
68-69	24.081947255865792	25.021492134786843	25.586415781731436	25.310144827615925
70-71	23.072436887268974	25.25008881434526	26.07716814321482	25.600306155170948
72-73	24.046768042626645	25.102419436570646	25.95031371390065	24.900498806902068
74-75	24.046165153327053	24.92524479557984	26.010267710436928	25.01832234065618
76-77	23.25314847652954	25.34274744497359	26.562276535756734	24.841827542740134
78-79	23.572433896926505	25.324090073673794	26.363869514828135	24.739606514571562
80-81	22.949702360028272	25.30994334747487	26.491083781911804	25.24927051058505
82-83	23.091007264873355	25.65330409930842	25.694504439644827	25.561184196173397
84-85	23.149463982332204	25.531148399533837	25.553821654261828	25.765565963872127
86-87	22.9764706023521	24.889096609842053	25.958363790608992	26.176068997196854
88-89	23.169152528025663	24.58577279795866	26.28826203362556	25.956812640390115
90-91	23.571274842645384	24.879924418733392	25.99465210833533	25.554148630285894
92-93	22.890218891138243	25.193623937727928	26.015892649972542	25.900264521161283
94-95	22.275348645218944	25.26754020098118	26.43567063050397	26.0214405232959
96-97	23.10674987778551	24.96802079176227	25.950220046077987	25.975009284374234
98-99	22.992645172712475	24.870237725196485	25.856286477359692	26.280830624731344
100	22.739516465698557	25.186989846091652	25.895057045170454	26.178436643039337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	514.0
1	471.0
2	556.5
3	644.5
4	499.0
5	446.5
6	585.0
7	1052.0
8	1662.0
9	1642.0
10	1118.0
11	854.5
12	921.0
13	1045.5
14	1243.0
15	1525.0
16	1817.0
17	2204.5
18	2696.5
19	3351.0
20	4286.0
21	5476.5
22	7120.5
23	9501.5
24	12633.0
25	17246.5
26	25047.5
27	34325.0
28	42772.0
29	54841.0
30	67724.5
31	80216.0
32	97262.5
33	115135.0
34	136122.0
35	152385.5
36	165509.5
37	183189.0
38	190716.0
39	192972.0
40	198380.5
41	204468.5
42	212056.5
43	232221.5
44	260247.5
45	283608.5
46	315396.0
47	353112.0
48	416568.5
49	477837.5
50	540037.0
51	525325.0
52	419331.0
53	366477.5
54	341988.0
55	318399.0
56	299461.0
57	275324.0
58	261849.0
59	242353.5
60	206819.0
61	167718.5
62	135182.0
63	104614.0
64	76928.0
65	63405.0
66	50176.0
67	35156.5
68	26366.5
69	21877.0
70	19854.0
71	18351.5
72	16057.5
73	17299.0
74	13888.0
75	9691.5
76	7052.5
77	4417.0
78	3046.0
79	1674.5
80	1104.0
81	846.0
82	631.0
83	533.0
84	412.0
85	277.0
86	208.0
87	111.5
88	50.5
89	32.0
90	26.5
91	22.5
92	21.0
93	11.5
94	7.0
95	7.0
96	7.5
97	4.5
98	2.5
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019176614217135995
2	0.00661036872331309
3	0.006850349106007625
4	0.004406912482208727
5	0.048912365272831514
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	3.9814927128865976E-4
18-19	0.0016744085792550483
20-21	6.326755543765004E-4
22-23	0.005023225737765145
24-25	0.007390304967070329
26-27	0.0057213504874219735
28-29	0.02268905436384691
30-31	0.02060013421448312
32-33	0.013318911239546672
34-35	0.013777055606508964
36-37	0.013875229399429458
38-39	0.021625504940541586
40-41	0.03100110216444852
42-43	0.01848394356708586
44-45	0.017284041653613188
46-47	0.01856575506118627
48-49	0.013760693307688884
50-51	0.010242799061371273
52-53	0.02804498017762039
54-55	0.026501469988926
56-57	0.013515258825387656
58-59	0.016602279202776443
60-61	0.01764401222765499
62-63	0.02148915245037424
64-65	0.011371797679956925
66-67	0.01566417407042508
68-69	0.007532111556844371
70-71	0.008971993853011579
72-73	0.010286431858224825
74-75	0.003583343441597938
76-77	0.007428483664317187
78-79	0.005672263590961728
80-81	0.0023616251296984885
82-83	1.7453118741420702E-4
84-85	0.002481615321045756
86-87	0.0022143644403177514
88-89	0.0011344527181923456
90-91	7.09032948870216E-4
92-93	0.0010526412240919362
94-95	0.003152469572669114
96-97	0.001434428196560514
98-99	0.0018707561650960314
100	0.0022579972371713033
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9167416.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.854012631490924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.92994938225306	32.246051490655056
2	11.829567221560318	9.665705773893924
3	3.6554529622205685	4.4801976447714
4	1.6802466412068073	2.7457927001553237
5	0.8722683925691628	1.7817831964035427
6	0.5538995206877151	1.3577410808851609
7	0.37234157138961194	1.0648153082546268
8	0.28064341112809443	0.9172327570537495
9	0.21411411708020062	0.7872678759397539
>10	1.3063748620742355	10.366859465245842
>50	0.1489136761807157	4.228162761168484
>100	0.12506374845863244	10.598600620629579
>500	0.01776644280202305	5.04452731036181
>1k	0.012454525723469362	9.669941551937717
>5k	6.200304945258461E-4	1.6215854617106178
>10k+	3.234941710569632E-4	3.4237350009334646
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	64606	0.7047351183801411	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	53497	0.5835559333186146	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	25896	0.28247872682989406	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	24183	0.26379298157736053	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	22519	0.24564173808628298	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	20357	0.22205821138693826	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	19108	0.20843387056941673	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	18732	0.20433238766518286	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	18508	0.20188895104138396	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	17129	0.186846544326122	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	14183	0.15471098944348113	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	12153	0.13256734504030362	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	9615	0.10488233543672504	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.454099606693969E-5	0.0	1.0908199213387939E-5	0.0
6	0.0	5.454099606693969E-5	0.0	1.0908199213387939E-5	0.0
7	0.0	5.454099606693969E-5	0.0	1.0908199213387939E-5	0.0
8	0.0	5.454099606693969E-5	0.0	1.0908199213387939E-5	0.0
9	0.0	5.454099606693969E-5	0.0	2.1816398426775878E-5	0.0
10-11	0.0	6.544919528032762E-5	0.0	5.454099606693969E-5	0.0
12-13	5.4540996066939695E-6	6.544919528032762E-5	0.0	7.09032948870216E-5	0.0
14-15	1.0908199213387939E-5	6.544919528032762E-5	0.0	8.72655937071035E-5	0.0
16-17	1.0908199213387939E-5	6.544919528032762E-5	0.0	1.418065897740432E-4	0.0
18-19	1.636229882008191E-5	6.544919528032762E-5	0.0	1.6362298820081907E-4	0.0
20-21	2.1816398426775878E-5	7.635739449371557E-5	0.0	2.781590799413924E-4	0.0
22-23	2.1816398426775878E-5	7.635739449371557E-5	0.0	7.19941148083604E-4	5.4540996066939695E-6
24-25	3.272459764016382E-5	7.635739449371557E-5	0.0	0.002307084133631549	1.0908199213387939E-5
26-27	6.544919528032762E-5	7.635739449371557E-5	0.0	0.004276014091648072	1.0908199213387939E-5
28-29	6.544919528032762E-5	7.635739449371557E-5	0.0	0.015560546177897894	1.0908199213387939E-5
30-31	6.544919528032762E-5	7.635739449371557E-5	0.0	0.05603541935917383	1.0908199213387939E-5
32-33	6.544919528032762E-5	7.635739449371557E-5	0.0	0.1310292889512159	1.0908199213387939E-5
34-35	6.544919528032762E-5	8.181149410040953E-5	0.0	0.22625241398448592	1.636229882008191E-5
36-37	6.544919528032762E-5	8.726559370710351E-5	0.0	0.37816545032973303	2.1816398426775878E-5
38-39	6.544919528032762E-5	8.726559370710351E-5	0.0	0.6286940616636139	2.7270498033469847E-5
40-41	6.544919528032762E-5	8.726559370710351E-5	0.0	0.9612414228829584	3.272459764016381E-5
42-43	7.635739449371557E-5	8.726559370710351E-5	0.0	1.2269597016214822	3.272459764016381E-5
44-45	7.635739449371557E-5	8.726559370710351E-5	0.0	1.5132072112796016	4.908689646024573E-5
46-47	7.635739449371557E-5	8.726559370710351E-5	0.0	1.8377152296786794	5.454099606693969E-5
48-49	7.635739449371557E-5	8.726559370710351E-5	1.0908199213387939E-5	2.1793109421455292	5.454099606693969E-5
50-51	7.635739449371557E-5	8.726559370710351E-5	1.0908199213387939E-5	2.543344820394318	5.454099606693969E-5
52-53	8.726559370710351E-5	8.726559370710351E-5	1.0908199213387939E-5	2.9219847773898335	5.999509567363366E-5
54-55	8.726559370710351E-5	9.271969331379748E-5	1.0908199213387939E-5	3.275972204163092	6.544919528032762E-5
56-57	8.726559370710351E-5	9.817379292049144E-5	1.0908199213387939E-5	3.670385417221167	6.544919528032762E-5
58-59	8.726559370710351E-5	9.817379292049144E-5	1.0908199213387939E-5	4.104040876949404	6.544919528032762E-5
60-61	8.726559370710351E-5	9.817379292049144E-5	1.0908199213387939E-5	4.541928717972436	6.544919528032762E-5
62-63	8.726559370710351E-5	9.817379292049144E-5	1.0908199213387939E-5	4.984245287876104	6.544919528032762E-5
64-65	8.726559370710351E-5	1.0362789252718542E-4	1.0908199213387939E-5	5.4372791635069255	6.544919528032762E-5
66-67	8.726559370710351E-5	1.0908199213387939E-4	1.0908199213387939E-5	5.918919791574856	6.544919528032762E-5
68-69	8.726559370710351E-5	1.0908199213387939E-4	1.0908199213387939E-5	6.489036823462577	6.544919528032762E-5
70-71	8.726559370710351E-5	1.0908199213387939E-4	1.0908199213387939E-5	7.165001566417407	6.544919528032762E-5
72-73	8.726559370710351E-5	1.0908199213387939E-4	1.0908199213387939E-5	7.770357535863978	6.544919528032762E-5
74-75	8.726559370710351E-5	1.0908199213387939E-4	1.0908199213387939E-5	8.359296665494398	6.544919528032762E-5
76-77	8.726559370710351E-5	1.0908199213387939E-4	1.0908199213387939E-5	8.981331271538238	8.726559370710351E-5
78-79	8.726559370710351E-5	1.0908199213387939E-4	1.0908199213387939E-5	9.618582815484757	8.726559370710351E-5
80-81	8.726559370710351E-5	1.0908199213387939E-4	1.0908199213387939E-5	10.326884915007675	9.271969331379748E-5
82-83	8.726559370710351E-5	1.1453609174057335E-4	1.0908199213387939E-5	11.110022715234042	9.817379292049144E-5
84-85	8.726559370710351E-5	1.1999019134726732E-4	1.0908199213387939E-5	11.85356375231581	9.817379292049144E-5
86-87	8.726559370710351E-5	1.418065897740432E-4	1.0908199213387939E-5	12.594797705263947	9.817379292049144E-5
88	8.726559370710351E-5	1.418065897740432E-4	1.0908199213387939E-5	13.172337766716378	9.817379292049144E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATA	2905	0.0	23.132605	1
ATGGGTA	5525	0.0	18.370783	5
GTATTAG	4695	0.0	18.21673	1
CATGGGT	7700	0.0	17.81981	4
TGATCGC	1700	0.0	17.411503	9
TATCACG	675	0.0	17.404493	2
GTACAAG	4610	0.0	17.227427	1
GTATAAG	3090	0.0	16.881039	1
GTATAAT	4100	0.0	16.73415	1
CATGGGG	21755	0.0	16.56713	4
GTACTAG	3275	0.0	16.501415	1
ATTGCGT	1210	0.0	16.308294	6
ATGGGAG	9115	0.0	16.290585	5
GTGATCG	1790	0.0	16.273586	8
GGTCGCC	5040	0.0	16.127773	94
GTACAAA	4940	0.0	16.076609	1
GTATATA	2920	0.0	15.932612	1
TAATACG	2400	0.0	15.859326	4
AATACGG	2150	0.0	15.517654	5
TAACGCA	550	0.0	15.37874	4
>>END_MODULE