##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765509_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6077020 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40897545178393 34.0 31.0 34.0 31.0 34.0 2 32.43716574900198 34.0 31.0 34.0 31.0 34.0 3 32.4724029211686 34.0 31.0 34.0 31.0 34.0 4 35.91897640619909 37.0 35.0 37.0 35.0 37.0 5 35.93194954105795 37.0 37.0 37.0 35.0 37.0 6 36.045592741179064 37.0 37.0 37.0 35.0 37.0 7 36.000840708110225 37.0 37.0 37.0 35.0 37.0 8 36.03766846250301 37.0 37.0 37.0 35.0 37.0 9 37.75914033523009 39.0 38.0 39.0 35.0 39.0 10-11 37.70176517437823 39.0 38.0 39.0 35.0 39.0 12-13 37.67953964278544 39.0 38.0 39.0 35.0 39.0 14-15 39.05822903330909 41.0 39.0 41.0 36.0 41.0 16-17 39.00642897341131 41.0 39.0 41.0 36.0 41.0 18-19 39.01515002418949 41.0 39.0 41.0 36.0 41.0 20-21 38.998599971696656 41.0 39.0 41.0 36.0 41.0 22-23 38.925674014566354 41.0 39.0 41.0 35.0 41.0 24-25 38.86112996172466 41.0 39.0 41.0 35.0 41.0 26-27 38.71563899740333 41.0 38.5 41.0 35.0 41.0 28-29 38.5451043439054 40.0 38.0 41.0 35.0 41.0 30-31 38.26798175750615 40.0 38.0 41.0 34.5 41.0 32-33 37.91414459718744 40.0 37.5 41.0 34.0 41.0 34-35 37.7805268865332 40.0 38.0 41.0 33.5 41.0 36-37 37.62401645872484 40.0 37.5 41.0 33.0 41.0 38-39 37.39544349039497 40.0 37.0 41.0 33.0 41.0 40-41 37.101676890976165 40.0 37.0 41.0 32.5 41.0 42-43 36.77654384879431 40.0 36.0 41.0 31.0 41.0 44-45 36.2861935619761 40.0 35.5 41.0 30.5 41.0 46-47 35.77412638102228 39.0 35.0 41.0 28.5 41.0 48-49 35.34384797153868 39.0 35.0 41.0 26.5 41.0 50-51 34.09129071485695 38.0 34.0 40.0 24.5 40.5 52-53 34.018443826085814 38.0 34.0 39.5 23.0 40.0 54-55 34.77191509983512 39.0 35.0 40.5 23.0 41.0 56-57 34.73930224024275 39.0 35.0 41.0 23.0 41.0 58-59 34.51858855162563 39.0 35.0 41.0 22.0 41.0 60-61 34.10925445366314 38.0 35.0 40.5 20.5 41.0 62-63 33.6619579991509 37.0 34.0 40.0 18.0 41.0 64-65 33.38977344158815 37.0 34.0 39.5 13.5 41.0 66-67 33.023933770170245 36.0 34.0 39.0 8.0 41.0 68-69 32.74404806961307 36.0 34.0 39.0 4.5 41.0 70-71 32.33064314417264 35.0 34.0 38.5 2.0 40.5 72-73 31.885847997867373 35.0 34.0 37.0 2.0 39.0 74-75 31.459708870466116 35.0 33.0 37.0 2.0 39.0 76-77 31.049332073944136 35.0 33.0 36.0 2.0 38.5 78-79 30.735154401334867 35.0 33.0 36.0 2.0 37.0 80-81 30.435310563401142 35.0 33.0 35.5 2.0 37.0 82-83 30.193896761899747 35.0 32.5 35.0 2.0 36.5 84-85 29.934957841178736 35.0 32.0 35.0 2.0 36.0 86-87 29.66833053042445 35.0 32.0 35.0 2.0 36.0 88-89 29.428211607004748 35.0 31.5 35.0 2.0 35.5 90-91 29.196297445129353 35.0 31.0 35.0 2.0 35.0 92-93 28.904538326350746 35.0 31.0 35.0 2.0 35.0 94-95 28.609524075945117 35.0 31.0 35.0 2.0 35.0 96-97 28.311477500485434 35.0 30.0 35.0 2.0 35.0 98-99 27.97957247137577 35.0 29.5 35.0 2.0 35.0 100 27.41628577822683 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4220153508230311 1101 2 0.5281185352822249 1101 3 0.5469598716407944 1101 4 0.46029049553655454 1101 5 0.4614285162050251 1101 6 0.4096758917833867 1101 7 0.4514935656323047 1101 8 0.3941194152640364 1101 9 0.46514644183927345 1101 10-11 0.4785596326470696 1101 12-13 0.5141059982108516 1101 14-15 0.6753686298765373 1101 16-17 0.7077772511278368 1101 18-19 0.6408866173819945 1101 20-21 0.6429453100440625 1101 22-23 0.6866943843585247 1101 24-25 0.6670986660899487 1101 26-27 0.7025393758634948 1101 28-29 0.668913844269774 1101 30-31 0.6659738776569668 1101 32-33 0.6929488201615115 1101 34-35 0.8174089733586101 1101 36-37 0.7523155274190358 1101 38-39 0.8485924568339911 1101 40-41 0.9339472650779399 1101 42-43 0.8070193008439688 1101 44-45 0.8590854572438715 1101 46-47 0.9105395467371338 1101 48-49 0.9191520073491404 1101 50-51 0.8501767402820306 1101 52-53 0.933531029987968 1101 54-55 1.0832188052725158 1101 56-57 1.154798940604607 1101 58-59 1.1013577690019432 1101 60-61 1.0779283704598512 1101 62-63 1.083371955894016 1101 64-65 1.0684845366085014 1101 66-67 1.021290202275864 1101 68-69 0.9713065872726361 1101 70-71 0.9127499533955543 1101 72-73 0.8837384369517025 1101 74-75 0.8042921845197135 1101 76-77 0.7820511904724903 1101 78-79 0.7353495240872796 1101 80-81 0.8023182952712844 1101 82-83 0.8383504154803809 1101 84-85 0.8401998391357708 1101 86-87 0.833132946567865 1101 88-89 0.8863966109807073 1101 90-91 0.9661156042785031 1101 92-93 0.9224700245483923 1101 94-95 0.7594147888659322 1101 96-97 0.7126578059775177 1101 98-99 0.6914977836013705 1101 100 0.6855425238523587 1102 1 0.452686407629713 1102 2 0.5812028179088671 1102 3 0.5762624561569183 1102 4 0.4440326407670412 1102 5 0.4433382498456311 1102 6 0.4663246639399574 1102 7 0.4749838808338538 1102 8 0.4277676490726563 1102 9 0.4314571127666724 1102 10-11 0.48803493268840015 1102 12-13 0.5380562524812404 1102 14-15 0.7077537307973287 1102 16-17 0.7102292612589594 1102 18-19 0.6529129933716504 1102 20-21 0.6611482823508439 1102 22-23 0.6811044010755509 1102 24-25 0.7026108980078405 1102 26-27 0.6687431116678155 1102 28-29 0.6707588248898659 1102 30-31 0.7303843647836104 1102 32-33 0.71391284772821 1102 34-35 0.753089988771869 1102 36-37 0.7799598743151677 1102 38-39 0.7523948485958414 1102 40-41 0.8531468718574686 1102 42-43 0.9186169002160369 1102 44-45 0.9511616039700499 1102 46-47 0.9040081758804419 1102 48-49 1.0485973454455646 1102 50-51 1.0210400931956585 1102 52-53 1.0050518284322223 1102 54-55 1.0881152622574533 1102 56-57 1.0767746417619506 1102 58-59 1.0755734855621384 1102 60-61 0.9779953338809619 1102 62-63 1.0220645983815686 1102 64-65 1.0556936138353379 1102 66-67 1.0480922929930472 1102 68-69 1.1088479765426342 1102 70-71 1.0817507727651687 1102 72-73 0.9639496814728119 1102 74-75 0.9603098951795026 1102 76-77 0.9786863670916297 1102 78-79 0.9603631226850062 1102 80-81 0.9636851447360968 1102 82-83 1.025917572355251 1102 84-85 1.0331238948256214 1102 86-87 0.9874424713745853 1102 88-89 0.9515196200353948 1102 90-91 0.9761538657180076 1102 92-93 1.1014252316724544 1102 94-95 1.1170255355747472 1102 96-97 1.1401526885241928 1102 98-99 1.1793609750193532 1102 100 1.1578314627297992 1103 1 0.43673326219371233 1103 2 0.484529087582537 1103 3 0.4988372324877659 1103 4 0.38532437034727707 1103 5 0.39757521470266255 1103 6 0.36930418477960814 1103 7 0.42521203003424546 1103 8 0.39559999815839575 1103 9 0.5040314924158764 1103 10-11 0.4323985322316517 1103 12-13 0.4499293554125998 1103 14-15 0.636801717744298 1103 16-17 0.5877782373336515 1103 18-19 0.5894518429408961 1103 20-21 0.5724867443007682 1103 22-23 0.6412526790396313 1103 24-25 0.574385784199059 1103 26-27 0.5938093350107039 1103 28-29 0.6071895071438576 1103 30-31 0.6327542227364091 1103 32-33 0.6493527255535412 1103 34-35 0.5534385206285535 1103 36-37 0.46737690344117055 1103 38-39 0.4159893249552127 1103 40-41 0.40511321051612725 1103 42-43 0.4895910988148202 1103 44-45 0.43285722008166516 1103 46-47 0.45821151276646077 1103 48-49 0.4777642959917898 1103 50-51 0.3750224375089459 1103 52-53 0.3442367613345496 1103 54-55 0.4483473872712196 1103 56-57 0.5245708870924517 1103 58-59 0.4786678191570104 1103 60-61 0.4076814057503455 1103 62-63 0.5098097936466814 1103 64-65 0.49534743671362946 1103 66-67 0.44610243561114515 1103 68-69 0.4059531656374986 1103 70-71 0.3930704966191314 1103 72-73 0.36670372764606185 1103 74-75 0.3818290280350567 1103 76-77 0.3497723767515204 1103 78-79 0.3705044444069898 1103 80-81 0.3785752160725444 1103 82-83 0.4170959982063316 1103 84-85 0.46368004903876425 1103 86-87 0.4555222353023858 1103 88-89 0.41919305109200167 1103 90-91 0.4105847203853834 1103 92-93 0.5676452334407536 1103 94-95 0.5039835958554697 1103 96-97 0.5356768436147767 1103 98-99 0.5187991503034013 1103 100 0.5937973935833618 1104 1 0.37290717696085096 1104 2 0.47310824992229783 1104 3 0.4920547680051328 1104 4 0.3882800668355699 1104 5 0.3741763179130757 1104 6 0.3532989341235506 1104 7 0.3475236842221605 1104 8 0.3000144916429761 1104 9 0.36363150430914004 1104 10-11 0.40460188062611735 1104 12-13 0.4158535006117461 1104 14-15 0.5145769504613398 1104 16-17 0.46603250431762433 1104 18-19 0.4663543523095939 1104 20-21 0.4752785780329347 1104 22-23 0.47444028317452336 1104 24-25 0.5151672377542056 1104 26-27 0.4539269241578836 1104 28-29 0.5259876927602747 1104 30-31 0.5370283096255122 1104 32-33 0.5892493422501417 1104 34-35 0.6139928568797757 1104 36-37 0.562739961860224 1104 38-39 0.660147584662262 1104 40-41 0.6996526603645421 1104 42-43 0.6839242886214194 1104 44-45 0.6543026481676151 1104 46-47 0.6276888808751195 1104 48-49 0.5771022375980621 1104 50-51 0.5516153916306763 1104 52-53 0.6042642700149798 1104 54-55 0.6450581287611996 1104 56-57 0.6671970080051111 1104 58-59 0.678419051706669 1104 60-61 0.7408860789223368 1104 62-63 0.7840673731250689 1104 64-65 0.7511010787505938 1104 66-67 0.7496895763492049 1104 68-69 0.7089566554296667 1104 70-71 0.6463353629245248 1104 72-73 0.6995202178776125 1104 74-75 0.7177955815321759 1104 76-77 0.6424237708513623 1104 78-79 0.707872310788364 1104 80-81 0.8069017786878305 1104 82-83 0.7039185914445731 1104 84-85 0.6979905755690012 1104 86-87 0.6797768965260502 1104 88-89 0.7281525336028487 1104 90-91 0.6643187099038919 1104 92-93 0.820374902841035 1104 94-95 0.867311265205096 1104 96-97 0.8541498365437903 1104 98-99 0.8073314003218464 1104 100 0.8711307034238267 1105 1 0.3095836540440615 1105 2 0.33958122316063566 1105 3 0.3611646682117353 1105 4 0.33756209661684267 1105 5 0.3047842663141651 1105 6 0.29885777130152746 1105 7 0.29597083526824264 1105 8 0.3127793100170635 1105 9 0.32011442347018004 1105 10-11 0.38033332968883116 1105 12-13 0.35506138210953964 1105 14-15 0.5006293872124061 1105 16-17 0.5262886040758872 1105 18-19 0.4963116299724035 1105 20-21 0.48659043731414187 1105 22-23 0.475145075993062 1105 24-25 0.4683365144648022 1105 26-27 0.4619809511541533 1105 28-29 0.49164137621396975 1105 30-31 0.5038272038952272 1105 32-33 0.5192768821552676 1105 34-35 0.5301119178771856 1105 36-37 0.5799654766142694 1105 38-39 0.5821749268202652 1105 40-41 0.5846408454903056 1105 42-43 0.5782646899791857 1105 44-45 0.5698675693426409 1105 46-47 0.48908502384146857 1105 48-49 0.6095271341683883 1105 50-51 0.5922906153967133 1105 52-53 0.565332269878553 1105 54-55 0.5946994106449139 1105 56-57 0.5926986018676388 1105 58-59 0.5993746492078955 1105 60-61 0.5532222269942864 1105 62-63 0.5697809009235257 1105 64-65 0.6155641970161199 1105 66-67 0.6062023815093696 1105 68-69 0.5869753394328541 1105 70-71 0.5539014471558446 1105 72-73 0.5855790452371394 1105 74-75 0.5910172095560391 1105 76-77 0.5901128682666261 1105 78-79 0.6083037026973379 1105 80-81 0.642651479741712 1105 82-83 0.5873342045795305 1105 84-85 0.6080872201722798 1105 86-87 0.6195681320563153 1105 88-89 0.6579718361342515 1105 90-91 0.734243214597285 1105 92-93 0.7460861293314345 1105 94-95 0.7276494610278093 1105 96-97 0.7672491541783906 1105 98-99 0.8015218893739267 1105 100 0.8007950121684182 1106 1 0.2381446972164767 1106 2 0.25232026829557697 1106 3 0.2936703376954668 1106 4 0.3041659893161608 1106 5 0.29502683776413363 1106 6 0.3116040547448762 1106 7 0.2815880647081386 1106 8 0.32244194819794103 1106 9 0.3528997434254464 1106 10-11 0.3387374463351165 1106 12-13 0.38120202936536884 1106 14-15 0.4933344941222231 1106 16-17 0.502941031206575 1106 18-19 0.4948664544937813 1106 20-21 0.46839440000476174 1106 22-23 0.46964106628491464 1106 24-25 0.4739935986601722 1106 26-27 0.4426854777404543 1106 28-29 0.4623474071095899 1106 30-31 0.40826768580291173 1106 32-33 0.36607443422231256 1106 34-35 0.32689744805809795 1106 36-37 0.3567150229611542 1106 38-39 0.33933345647435686 1106 40-41 0.29806639697073223 1106 42-43 0.2783260897169768 1106 44-45 0.27134912518871346 1106 46-47 0.34924531146037197 1106 48-49 0.3322764451129032 1106 50-51 0.22025858934383535 1106 52-53 0.18475529487464115 1106 54-55 0.20267369765580412 1106 56-57 0.19723698976418547 1106 58-59 0.19455472258545825 1106 60-61 0.10741943879789062 1106 62-63 0.10900727681580946 1106 64-65 0.08810362848790732 1106 66-67 0.07627100067301029 1106 68-69 0.10085690434254246 1106 70-71 0.07864736679551498 1106 72-73 0.006670877970528011 1106 74-75 0.0647790654331395 1106 76-77 0.06843926436322789 1106 78-79 0.12692362164087356 1106 80-81 0.11440725418091802 1106 82-83 0.0908028019653031 1106 84-85 0.05587173132525436 1106 86-87 0.08310474436433424 1106 88-89 0.16946381514441455 1106 90-91 0.14496698878975778 1106 92-93 0.16280992618296253 1106 94-95 0.06397544674379319 1106 96-97 0.09540774740371205 1106 98-99 0.08916235189553845 1106 100 0.16619388387349332 1107 1 0.23003819601393616 1107 2 0.28000500194783484 1107 3 0.3234463138580139 1107 4 0.3234085487160314 1107 5 0.3263274790489916 1107 6 0.2935130362920617 1107 7 0.34153140933032944 1107 8 0.3071089734588881 1107 9 0.3880429469079445 1107 10-11 0.3644533545520545 1107 12-13 0.3315257381234602 1107 14-15 0.3864970135317307 1107 16-17 0.40389590800122477 1107 18-19 0.3982689497362486 1107 20-21 0.37881712198324635 1107 22-23 0.34837255151391133 1107 24-25 0.371479007920108 1107 26-27 0.3592353248342164 1107 28-29 0.39020240319891286 1107 30-31 0.3845322456288329 1107 32-33 0.3138981549328861 1107 34-35 0.3114888922850483 1107 36-37 0.32859901112058054 1107 38-39 0.35217275880091137 1107 40-41 0.35922566365113795 1107 42-43 0.32741945374940684 1107 44-45 0.3444713998372677 1107 46-47 0.2917770169810794 1107 48-49 0.3310896812200852 1107 50-51 0.4024326681951962 1107 52-53 0.42457733916777585 1107 54-55 0.4091972351126216 1107 56-57 0.3498759571968151 1107 58-59 0.2927133254158889 1107 60-61 0.29619307587352495 1107 62-63 0.32982396692607097 1107 64-65 0.3914786979377638 1107 66-67 0.3237209744985421 1107 68-69 0.34491502801695617 1107 70-71 0.31093990512064096 1107 72-73 0.31329792396329026 1107 74-75 0.30895286261897326 1107 76-77 0.35142715410357184 1107 78-79 0.3706584231685035 1107 80-81 0.40410236306394154 1107 82-83 0.32164709730304963 1107 84-85 0.33910504546181386 1107 86-87 0.35281892528461256 1107 88-89 0.38367595944947297 1107 90-91 0.2980930864633571 1107 92-93 0.2690514646338755 1107 94-95 0.26617280372727237 1107 96-97 0.2238269923595091 1107 98-99 0.2642725110312405 1107 100 0.3295278922518037 1108 1 0.16157927732918154 1108 2 0.17254983025441817 1108 3 0.20756243976224908 1108 4 0.1351569915631572 1108 5 0.1821264143289838 1108 6 0.15807163312597083 1108 7 0.18024514837875927 1108 8 0.20329754145674173 1108 9 0.21970180951063156 1108 10-11 0.224796033646669 1108 12-13 0.23518178353113228 1108 14-15 0.2578850032308466 1108 16-17 0.23590150703225277 1108 18-19 0.2696295390052228 1108 20-21 0.30805951841283985 1108 22-23 0.2513579647501629 1108 24-25 0.2474744519796772 1108 26-27 0.26084500988899606 1108 28-29 0.17243833088907934 1108 30-31 0.2782864833242513 1108 32-33 0.2905567789182584 1108 34-35 0.28594468393593075 1108 36-37 0.3159253261020467 1108 38-39 0.2709959283696506 1108 40-41 0.284754021991354 1108 42-43 0.23533613316751456 1108 44-45 0.18205805666980268 1108 46-47 0.182252462419207 1108 48-49 0.29718379592099353 1108 50-51 0.19780807422927893 1108 52-53 0.26573131101048375 1108 54-55 0.3036601914374799 1108 56-57 0.3325308834808425 1108 58-59 0.3620303713031987 1108 60-61 0.385767361866435 1108 62-63 0.3525839195203915 1108 64-65 0.3040918166762552 1108 66-67 0.2003297322187123 1108 68-69 0.18006447323587338 1108 70-71 0.17490654636277014 1108 72-73 0.25181026775216253 1108 74-75 0.2630690869913188 1108 76-77 0.22780774274929527 1108 78-79 0.2396354267805485 1108 80-81 0.23278111068609064 1108 82-83 0.20945123158736934 1108 84-85 0.15909557685783682 1108 86-87 0.15720704472665403 1108 88-89 0.25281395282715735 1108 90-91 0.22082783178090182 1108 92-93 0.18684741879182454 1108 94-95 0.20005611129571932 1108 96-97 0.26037483020961005 1108 98-99 0.17526640281937844 1108 100 0.23908207956063876 1109 1 0.07255826431777024 1109 2 0.12519791244348966 1109 3 0.1865215692759179 1109 4 0.14284138946768365 1109 5 0.22357113974067744 1109 6 0.18438038114148014 1109 7 0.16398529009105545 1109 8 0.17721728059745345 1109 9 0.1692215480096806 1109 10-11 0.19101046721187487 1109 12-13 0.23604062894644073 1109 14-15 0.2608441872862599 1109 16-17 0.2978600531630775 1109 18-19 0.17353224186978622 1109 20-21 0.2252400895719049 1109 22-23 0.3219180924848004 1109 24-25 0.3154008548389484 1109 26-27 0.2964263367186746 1109 28-29 0.2912490854528116 1109 30-31 0.32015291806370527 1109 32-33 0.34558937830856706 1109 34-35 0.33785471464506855 1109 36-37 0.35929935898858645 1109 38-39 0.381842571405123 1109 40-41 0.31426121588781797 1109 42-43 0.2888308958226631 1109 44-45 0.2565296834630644 1109 46-47 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-0.590939735904751 2316 100 -0.6864543813514707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17121.0 3 2492.0 4 2393.0 5 2672.0 6 3201.0 7 3682.0 8 4873.0 9 5998.0 10 8361.0 11 14503.0 12 24464.0 13 37633.0 14 37387.0 15 43770.0 16 58765.0 17 82132.0 18 81715.0 19 45345.0 20 37913.0 21 34556.0 22 34814.0 23 39501.0 24 43805.0 25 43689.0 26 45795.0 27 51190.0 28 59834.0 29 69871.0 30 83684.0 31 104148.0 32 133665.0 33 180471.0 34 264389.0 35 425677.0 36 789671.0 37 1555973.0 38 1354294.0 39 247314.0 40 259.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.105837833991906 17.096617358158433 13.485044725500453 23.312500082349207 2 13.801326247428674 22.468517877410065 36.99859375397064 26.73156212119062 3 19.36418731800436 28.65574635084617 29.26828141331752 22.711784917831952 4 13.071762751310073 18.561368400188996 33.890880561217564 34.47598828728337 5 12.76301384516238 36.7158611891732 33.27632746635113 17.24479749931329 6 29.931671716330072 33.160588197188574 18.8987959103578 18.008944176123556 7 24.120054588252053 32.89644487249277 22.11356704024911 20.869933499006063 8 27.985018420434173 30.40975206824048 21.736161766252156 19.86906774507319 9 28.316581054411667 14.715502559298846 19.34446729599767 37.623449090291814 10-11 24.172905642995456 26.57106718551465 25.547262941094935 23.70876423039496 12-13 27.425811132878447 24.201271038618575 26.189163693932688 22.183754134570293 14-15 26.38406976298568 22.438308984390936 25.754317227578994 25.42330402504439 16-17 22.469379748148743 26.230148000871207 28.414606479629228 22.885865771350822 18-19 24.298146853838496 24.99275522382155 30.190919221157714 20.51817870118224 20-21 23.68115656217053 24.549508178123713 29.587075408510444 22.182259851195315 22-23 22.12951771034929 26.41386010565534 26.358146190346755 25.098475993648613 24-25 22.29077441398304 27.30743520156787 25.574343978139876 24.82744640630921 26-27 23.615889935455886 24.69801388584223 26.32974831859437 25.356347860107515 28-29 22.927438816333805 23.816108695906717 29.882773944290165 23.37367854346931 30-31 25.12201181581165 24.146560672338556 28.471175120939513 22.260252390910285 32-33 20.594495709397602 25.92200846287463 29.242155060346324 24.241340767381445 34-35 20.196302434922792 26.68231860775292 29.516387321927724 23.60499163539657 36-37 22.920601480415563 26.603372916440414 27.413890303833398 23.062135299310615 38-39 24.25928845498368 24.45004178649498 27.00115961024312 24.28951014827822 40-41 22.438784935693246 26.35766094808554 26.859192277012195 24.34436183920902 42-43 25.114370971191104 25.21736580357387 26.53534214581821 23.132921079416814 44-45 24.901185054342452 24.405981624407637 27.61669292065145 23.076140400598458 46-47 23.016394556258422 25.09280641368185 29.504554025084794 22.38624500497493 48-49 23.66103887677357 26.136751971861795 28.458531686111773 21.743677465252865 50-51 22.27799389196742 26.827844126359317 26.67321811329056 24.220943868382708 52-53 21.542866215394636 27.69949392354796 24.48579351622657 26.271846344830834 54-55 22.70587845845441 26.73439854069512 24.32484421762299 26.23487878322748 56-57 23.68723875610734 24.527576967297644 25.141781484715853 26.643402791879154 58-59 23.484064937424378 24.158338424550223 27.834206633234228 24.523390004791167 60-61 23.690200658121537 25.157468844171888 26.604267007191197 24.54806349051538 62-63 20.410627292920072 25.80739088260785 27.780994273008957 26.000987551463123 64-65 20.488807194959872 25.831527001486386 27.49098550143264 26.1886803021211 66-67 23.039431433859615 25.429031335208336 25.86521892453795 25.666318306394103 68-69 23.90012776910271 23.968464019033007 25.890057623346436 26.241350588517847 70-71 22.39868719921195 24.982157749468357 26.31593342836568 26.30322162295401 72-73 24.37123830510952 24.05437812481433 26.218573039806692 25.35581053026945 74-75 24.55530782082346 23.218521241002353 26.89711409966377 25.329056838510418 76-77 22.663917935659388 24.702726769071816 28.314421178272948 24.31893411699585 78-79 23.039008215353824 24.436897815652717 28.095530713637864 24.42856325535559 80-81 22.177833153840652 24.80589917595894 27.256789616680727 25.759478053519675 82-83 22.15293132307606 25.617127477649504 25.314822619390437 26.915118579883995 84-85 22.751201868691627 25.29029757814671 25.223084087908816 26.735416465252854 86-87 23.26586204051278 23.419454802982433 26.08742530044477 27.227257856060017 88-89 23.487546976989517 22.658567943561682 27.6019730335597 26.251912045889103 90-91 24.118028995101216 23.483061630290784 26.675379383975667 25.723529990632333 92-93 22.544782881151537 24.088618499056896 27.226309583817887 26.14028903597368 94-95 22.20018833809959 24.173254536530393 27.51243256062525 26.114124564744774 96-97 24.103591842262155 23.751310876268146 25.957951218062096 26.187146063407603 98-99 24.80586456431933 22.84058948008458 25.733539371303305 26.62000658429279 100 24.407607691389746 23.192659270667328 26.44021831651929 25.959514721423638 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1688.0 1 2502.5 2 2092.0 3 768.5 4 590.5 5 604.0 6 771.5 7 1162.5 8 1681.5 9 1819.5 10 1569.5 11 1471.5 12 1689.0 13 1953.0 14 2255.0 15 2607.5 16 2985.5 17 3491.5 18 4124.5 19 4785.0 20 5642.0 21 6784.5 22 8309.0 23 10212.5 24 12599.5 25 15606.0 26 19453.0 27 24348.5 28 29922.0 29 36749.0 30 44753.0 31 52525.5 32 61238.5 33 70096.0 34 78363.0 35 84820.5 36 89603.0 37 96020.0 38 99921.0 39 100223.5 40 99486.5 41 99390.0 42 102199.5 43 111670.0 44 124801.5 45 137244.5 46 160347.0 47 197765.5 48 318346.5 49 461680.0 50 614801.0 51 580091.0 52 363085.5 53 271311.0 54 224270.5 55 180442.5 56 155894.5 57 136778.0 58 125583.5 59 115394.0 60 99113.5 61 83004.0 62 68732.0 63 54150.0 64 40463.0 65 32407.5 66 25236.0 67 18708.0 68 14805.5 69 12410.0 70 10825.5 71 9236.5 72 7760.5 73 7678.5 74 6445.5 75 5115.0 76 3972.0 77 2631.5 78 1836.5 79 1305.0 80 939.0 81 702.5 82 555.5 83 410.5 84 275.5 85 180.0 86 127.5 87 93.5 88 71.0 89 56.0 90 43.5 91 38.0 92 35.5 93 32.0 94 26.5 95 18.0 96 13.0 97 11.5 98 7.0 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08747708580850483 2 0.27832720642683423 3 0.28174993664658005 4 0.28999410895471794 5 0.3153519323615851 6 0.16914540350369095 7 0.18505780793875948 8 0.15306844473113465 9 0.20195753839875466 10-11 0.2214654551079312 12-13 0.23727089922363265 14-15 0.16772200848442165 16-17 0.15779938193390838 18-19 0.17409848906207318 20-21 0.20447521976231772 22-23 0.1697048882511494 24-25 0.19303046559004247 26-27 0.191434288516411 28-29 0.21969649597993754 30-31 0.25196560156129155 32-33 0.22177810834915798 34-35 0.15120898071752273 36-37 0.14577868758042595 38-39 0.11316401789034758 40-41 0.10387492553916228 42-43 0.1200917554985832 44-45 0.1332890133651033 46-47 0.1992835304145782 48-49 0.20377586382799465 50-51 0.20915679066384513 52-53 0.1393857515690256 54-55 0.0837252469137834 56-57 0.12144932878285737 58-59 0.1408996514739132 60-61 0.11796077682811643 62-63 0.13946802873776948 64-65 0.11415957163214865 66-67 0.11773040075563353 68-69 0.10372682663542328 70-71 0.12931502611477336 72-73 0.1540146321716894 74-75 0.19115454614268176 76-77 0.17217320331346614 78-79 0.19603358224919448 80-81 0.10212242184491742 82-83 0.14221608617381545 84-85 0.1732674896577599 86-87 0.18602045081306298 88-89 0.16817453291251305 90-91 0.1712846098910321 92-93 0.12225564503654753 94-95 0.3612296816531787 96-97 0.36053855343573 98-99 0.4070662923604003 100 0.4397221006348507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6077020.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.5426873459638 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5571460665623 27.48117622714435 2 10.971426711670006 7.579651252807454 3 3.417033805520395 3.5410059118403954 4 1.6095572407364904 2.223937301287711 5 0.9180736281860357 1.5856365149502418 6 0.6293627006302528 1.304392739704934 7 0.43775423590992685 1.0584845393785516 8 0.3154247487537649 0.8716494781904387 9 0.2553176213352349 0.7937421090928374 >10 1.5780443021482886 10.260627613136892 >50 0.1536171564591035 3.6816467647595728 >100 0.1308472288070263 9.249157811163146 >500 0.013725167957243264 3.2696445682247934 >1k 0.009933949856263554 6.506134805365404 >5k 0.0010557821103275222 2.6680460679425306 >10k+ 0.00167965335733924 17.925066295010893 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 134659 2.2158722531767214 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 111719 1.83838460297975 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 72106 1.1865355058893996 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 53788 0.885104870479281 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 53320 0.8774037274848527 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 49347 0.812026289200957 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 47897 0.7881659102652286 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 47744 0.7856482289016655 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 43377 0.7137873497207513 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 41974 0.6907003761712155 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 37694 0.6202711197264449 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 34433 0.5666099502716792 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 22871 0.37635222526830586 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22625 0.3723041885661064 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 21717 0.3573626547222158 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 20690 0.34046292426222063 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 19007 0.31276842926302695 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 18970 0.31215957821432216 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 17556 0.2888915948935498 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 16067 0.26438945404161907 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 15885 0.26139456509934145 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15857 0.2609338129543756 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 15202 0.25015550384892593 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 13828 0.22754573787810473 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 13752 0.2262951249131976 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 13310 0.2190218231962376 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 13291 0.21870916995501083 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 12935 0.21285103554044582 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 12581 0.2070258119933783 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 11770 0.19368045522311922 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 11582 0.19058683367834894 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 11206 0.18439959058880834 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 11020 0.18133887991153558 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10769 0.17720856604059226 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 10768 0.1771921106068435 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 9615 0.15821899549450225 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 9516 0.1565899075533732 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 9133 0.15028747642759116 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 8959 0.14742423095530374 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 8603 0.1415660965407387 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 8554 0.14075978028704858 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 8375 0.1378142576460173 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 7920 0.13032703529032322 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 7905 0.13008020378409155 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 7812 0.12854984844545517 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 7285 0.11987783485984907 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 7231 0.11898924143741506 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 7075 0.11642219377260565 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 7044 0.11591207532639353 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA 6802 0.11192986035918921 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 6373 0.10487047928096337 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 6287 0.10345531197856846 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1518803624144728E-4 0.0 0.0 0.0 1.6455433748778184E-5 2 2.961978074780073E-4 0.0 0.0 0.0 1.6455433748778184E-5 3 3.126532412267855E-4 0.0 0.0 0.0 1.6455433748778184E-5 4 3.9493040997067643E-4 0.0 0.0 0.0 1.6455433748778184E-5 5 0.002040473784848495 0.0 0.0 0.0 1.6455433748778184E-5 6 0.0046075214496578915 0.0 0.0 0.0 1.6455433748778184E-5 7 0.005051818160874902 0.0 0.0 3.291086749755637E-5 1.6455433748778184E-5 8 0.0055619366070870264 0.0 0.0 6.582173499511274E-5 1.6455433748778184E-5 9 0.0062201539570381536 0.0 0.0 1.4809890373900365E-4 1.6455433748778184E-5 10-11 0.010885269424816768 0.0 0.0 3.044255243523964E-4 1.6455433748778184E-5 12-13 0.022074964373985935 0.0 0.0 4.3606899434262187E-4 1.6455433748778184E-5 14-15 0.033725411468120886 0.0 0.0 5.348015968352909E-4 8.227716874389092E-5 16-17 0.037551299814711815 0.0 0.0 8.556825549364656E-4 1.234157531158364E-4 18-19 0.03965759533455543 0.0 0.0 0.0010531477599218038 1.4809890373900365E-4 20-21 0.04800050024518596 0.0 0.0 0.001966424332978993 1.5632662061339274E-4 22-23 0.051521963067424494 0.0 0.0 0.005018907293377346 1.8100977123656E-4 24-25 0.07208302753652283 0.0 0.0 0.011996011202859296 1.9746520498533821E-4 26-27 0.13975599882837314 0.0 0.0 0.023514814827004023 2.2214835560850548E-4 28-29 0.15449183975040398 0.0 0.0 0.06916218804611471 2.3037607248289457E-4 30-31 0.15790634225327543 0.0 0.0 0.17729084320933616 2.3037607248289457E-4 32-33 0.16036642959871777 0.0 0.0 0.33546870011946645 2.3037607248289457E-4 34-35 0.16211893329296267 0.0 0.0 0.532580113279206 2.468315062316728E-4 36-37 0.16541002004271832 0.0 0.0 0.8220641037877118 2.6328693998045095E-4 38-39 0.16902198775057511 0.0 0.0 1.2615064620488332 2.879700906036182E-4 40-41 0.1720497875603503 0.0 0.0 1.9471550200591738 2.961978074780073E-4 42-43 0.17980852457289923 0.0 0.0 2.4139874477951366 3.126532412267855E-4 44-45 0.18866977564661627 0.0 0.0 2.85939490079019 3.126532412267855E-4 46-47 0.19242984225821208 0.0 0.0 3.3796334387578124 3.208809581011746E-4 48-49 0.19480765243491055 0.0 0.0 3.841183672260417 3.2910867497556366E-4 50-51 0.19854303589588318 0.0 0.0 4.372760004080948 3.4556410872434184E-4 52-53 0.20021326242138415 0.0 0.0 5.062292044456 3.7847497622189825E-4 54-55 0.20503470450977618 0.0 0.0 5.621900207667574 4.113858437194546E-4 56-57 0.2166357853026648 0.0 0.0 6.17973118403428 4.278412774682328E-4 58-59 0.22051926766737645 0.0 0.0 6.757802343911983 4.278412774682328E-4 60-61 0.22279011752470784 0.0 0.0 7.256821600060556 4.278412774682328E-4 62-63 0.2245673043695759 0.0 0.0 7.775538010406416 4.6075214496578914E-4 64-65 0.22608943199133785 0.0 0.0 8.34639346258528 4.6075214496578914E-4 66-67 0.2280969949086888 0.0 0.0 8.910526870077769 4.6075214496578914E-4 68-69 0.23025265672977874 3.291086749755637E-5 0.0 9.606155648656742 4.6075214496578914E-4 70-71 0.2330583081839454 3.291086749755637E-5 0.0 10.492560498402177 4.772075787145673E-4 72-73 0.2400107289428042 4.113858437194546E-5 0.0 11.167759855982045 5.018907293377346E-4 74-75 0.24700428828603493 4.9366301246334553E-5 0.0 11.80498007246973 5.101184462121237E-4 76-77 0.2499004446258199 6.582173499511274E-5 0.0 12.484877456384872 5.101184462121237E-4 78-79 0.2516282651694416 8.227716874389092E-5 0.0 13.11373831252818 5.101184462121237E-4 80-81 0.25459024324422164 8.227716874389092E-5 0.0 13.840541910344214 5.101184462121237E-4 82-83 0.25589022251037513 8.227716874389092E-5 0.0 14.753283681804568 5.594847474584583E-4 84-85 0.26068698144814395 8.227716874389092E-5 0.0 15.555074691213786 5.759401812072364E-4 86-87 0.27000075694995246 9.050488561828E-5 0.0 16.31443536470178 5.923956149560146E-4 88 0.2723867948435253 1.1518803624144728E-4 0.0 16.923130086785957 5.923956149560146E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 9530 0.0 31.32392 1 ATCTACA 10585 0.0 28.270998 2 TCTACAC 12430 0.0 25.849321 3 AGTACTC 2990 0.0 25.33362 5 TACACTC 13275 0.0 24.135437 5 CTACACT 14375 0.0 22.845701 4 TATCACG 410 0.0 20.65682 2 CACTCTT 15590 0.0 20.565382 7 ACACTCT 15705 0.0 20.414623 6 TAGTACT 1250 0.0 19.949076 4 AAAGTAC 3525 0.0 19.885052 3 ACTCTTT 17620 0.0 19.503386 8 AAGTACT 3885 0.0 19.498075 4 GATGTAA 2165 0.0 18.92087 1 AGTACTT 33885 0.0 18.621395 12-13 GTGATCG 725 0.0 18.155931 8 GTATAAG 1300 0.0 18.109497 1 CGTTAAC 370 1.6189006E-10 17.815828 1 ATGTAAG 2325 0.0 17.606422 2 GAGTACT 33525 0.0 17.17405 12-13 >>END_MODULE