##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765509_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6077020 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.555270017212386 34.0 31.0 34.0 31.0 34.0 2 32.67145031610888 34.0 31.0 34.0 31.0 34.0 3 32.725499340137105 34.0 31.0 34.0 31.0 34.0 4 36.214216836541596 37.0 35.0 37.0 35.0 37.0 5 36.145715992377845 37.0 35.0 37.0 35.0 37.0 6 36.236532050248314 37.0 37.0 37.0 35.0 37.0 7 36.16697147615114 37.0 37.0 37.0 35.0 37.0 8 36.19240334900988 37.0 37.0 37.0 35.0 37.0 9 37.937952154180834 39.0 38.0 39.0 35.0 39.0 10-11 37.87887714702272 39.0 38.0 39.0 35.0 39.0 12-13 37.86956279558073 39.0 38.0 39.0 35.0 39.0 14-15 39.18443957729282 40.5 39.0 41.0 36.0 41.0 16-17 39.15426746991124 40.0 38.5 41.0 36.0 41.0 18-19 39.19804591724233 40.0 39.0 41.0 36.0 41.0 20-21 39.211520860553364 40.0 39.0 41.0 36.0 41.0 22-23 39.16068796877417 40.0 39.0 41.0 35.5 41.0 24-25 39.113480291326994 40.0 39.0 41.0 35.0 41.0 26-27 38.9464986950841 40.0 38.0 41.0 35.0 41.0 28-29 38.79480427907099 40.0 38.0 41.0 35.0 41.0 30-31 38.56025683640995 40.0 38.0 41.0 35.0 41.0 32-33 38.19726938532373 40.0 37.5 41.0 34.0 41.0 34-35 38.14735972565501 40.0 38.0 41.0 34.0 41.0 36-37 38.037904104314286 40.0 38.0 41.0 34.0 41.0 38-39 37.81932904614433 40.0 37.5 41.0 33.0 41.0 40-41 37.53674917969663 40.0 37.0 41.0 33.0 41.0 42-43 37.222570108375486 40.0 37.0 41.0 33.0 41.0 44-45 36.7771614212229 40.0 36.0 41.0 31.5 41.0 46-47 36.36229006980395 39.0 36.0 41.0 31.0 41.0 48-49 36.03197002807297 39.0 35.0 41.0 30.0 41.0 50-51 35.74915814000941 39.0 35.0 41.0 28.5 41.0 52-53 35.59678304497928 39.0 35.0 41.0 28.0 41.0 54-55 35.54514391922356 39.0 35.0 41.0 28.0 41.0 56-57 35.34104232008451 39.0 35.0 41.0 28.0 41.0 58-59 35.21262131768531 39.0 35.0 41.0 28.0 41.0 60-61 34.84129474643822 38.0 35.0 40.5 27.0 41.0 62-63 34.4303128836173 37.0 35.0 40.0 26.0 41.0 64-65 34.23331542433627 37.0 35.0 40.0 26.0 41.0 66-67 33.81791618260266 36.5 35.0 39.0 26.0 41.0 68-69 33.46000770114299 36.0 34.0 39.0 25.5 41.0 70-71 33.04415346666623 35.0 34.0 38.5 24.5 40.5 72-73 32.603243941932064 35.0 34.0 37.0 23.5 39.0 74-75 32.224576683966816 35.0 34.0 37.0 21.5 39.0 76-77 30.805661245149764 34.0 31.5 35.0 20.0 37.0 78-79 31.53821897903907 35.0 33.0 36.0 20.0 37.0 80-81 31.478202227407515 35.0 33.0 35.0 20.0 37.0 82-83 31.313506126357986 35.0 33.5 35.0 18.5 36.5 84-85 31.115827576674093 35.0 33.0 35.0 16.5 36.0 86-87 30.936920069376107 35.0 33.0 35.0 11.0 36.0 88-89 30.779803176556932 35.0 33.0 35.0 4.5 35.5 90-91 30.64650412537724 35.0 33.0 35.0 2.0 35.0 92-93 30.48276260403948 35.0 33.0 35.0 2.0 35.0 94-95 30.408016017719213 35.0 33.0 35.0 2.0 35.0 96-97 30.310308917857768 35.0 33.0 35.0 2.0 35.0 98-99 30.175720007503678 35.0 33.0 35.0 2.0 35.0 100 29.84343230728219 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4011394121145031 1101 2 0.31948466472852033 1101 3 0.34274224913482954 1101 4 0.23637989299469808 1101 5 0.27502971266738285 1101 6 0.23418160236587937 1101 7 0.2802968090754092 1101 8 0.29552936465475454 1101 9 0.3039418425128204 1101 10-11 0.3242643736826736 1101 12-13 0.34194217002883676 1101 14-15 0.5208236060872835 1101 16-17 0.4792706879271549 1101 18-19 0.46884798038288267 1101 20-21 0.45640346753314276 1101 22-23 0.4637336325211123 1101 24-25 0.4795527157434094 1101 26-27 0.5085105824459077 1101 28-29 0.5293404480135209 1101 30-31 0.6105350103396816 1101 32-33 0.5456357201191366 1101 34-35 0.5472070399731379 1101 36-37 0.6392913930775279 1101 38-39 0.661350353005524 1101 40-41 0.7000964688485922 1101 42-43 0.7467355649089029 1101 44-45 0.7873271914375266 1101 46-47 0.7983040173105422 1101 48-49 0.7619566629753507 1101 50-51 0.7577978217852532 1101 52-53 0.8646037046579593 1101 54-55 0.9449231046722915 1101 56-57 0.8445730906049107 1101 58-59 0.9044444609034841 1101 60-61 0.8581628992051265 1101 62-63 0.8863283629940426 1101 64-65 1.0003517733916425 1101 66-67 0.9660640104627376 1101 68-69 0.8963781367742385 1101 70-71 0.946260472351149 1101 72-73 0.9580987976381792 1101 74-75 0.9288272682148246 1101 76-77 0.918299129290947 1101 78-79 0.9515262785722065 1101 80-81 0.9499849920721601 1101 82-83 1.0196814108101648 1101 84-85 0.9943169261473095 1101 86-87 0.9235809898013585 1101 88-89 0.9817157379007533 1101 90-91 1.0394488813222544 1101 92-93 1.0333482705501424 1101 94-95 1.0721646140073346 1101 96-97 1.1155981219503097 1101 98-99 1.1048749559606108 1101 100 1.1450865572314868 1102 1 0.3662073447717802 1102 2 0.2936637234388755 1102 3 0.3282525399572691 1102 4 0.2675259498472897 1102 5 0.26773320482924845 1102 6 0.2882179835658931 1102 7 0.28456612610097665 1102 8 0.27788047891785794 1102 9 0.3502677903860132 1102 10-11 0.34694828176533576 1102 12-13 0.3628380406630356 1102 14-15 0.4330461988469452 1102 16-17 0.44014552652987504 1102 18-19 0.45458457932658547 1102 20-21 0.43561418227509563 1102 22-23 0.44786111142897056 1102 24-25 0.47812013046920754 1102 26-27 0.45792650944882496 1102 28-29 0.5345879197585575 1102 30-31 0.5774552901617156 1102 32-33 0.5952590820247536 1102 34-35 0.5593088520032197 1102 36-37 0.6342713879904593 1102 38-39 0.6993221146134729 1102 40-41 0.6849775868076549 1102 42-43 0.7158777869613004 1102 44-45 0.7663039692033422 1102 46-47 0.7853202528252297 1102 48-49 0.7896587181145236 1102 50-51 0.896904678302711 1102 52-53 0.8386194315531768 1102 54-55 0.9443293436853182 1102 56-57 0.9855145009121742 1102 58-59 0.9923835823794178 1102 60-61 0.989483055706387 1102 62-63 1.0405676105275248 1102 64-65 1.0395312329994084 1102 66-67 1.0167281755062092 1102 68-69 0.9625730327249684 1102 70-71 0.9729266645278116 1102 72-73 0.9907589745174405 1102 74-75 0.9454507615378489 1102 76-77 0.8738928985844083 1102 78-79 0.9763691088607409 1102 80-81 1.0439097699019797 1102 82-83 1.029511856997189 1102 84-85 1.0590099213546402 1102 86-87 1.0711651872209131 1102 88-89 1.0585142611992744 1102 90-91 1.0561681615850453 1102 92-93 1.0961139634543287 1102 94-95 1.0326645239037795 1102 96-97 1.0582697259360607 1102 98-99 1.0916059728559233 1102 100 1.1412810046317787 1103 1 0.306400879613399 1103 2 0.2715847500935098 1103 3 0.2937576355170606 1103 4 0.19836671291882624 1103 5 0.19816456790345427 1103 6 0.1760080733787106 1103 7 0.19739525225056553 1103 8 0.24770556030507862 1103 9 0.2599905311797457 1103 10-11 0.298574243033336 1103 12-13 0.3131277164138311 1103 14-15 0.40165422413380014 1103 16-17 0.409754539104199 1103 18-19 0.3666579451474732 1103 20-21 0.3671385259734805 1103 22-23 0.3653510015677881 1103 24-25 0.40431757127551293 1103 26-27 0.43138809827414804 1103 28-29 0.4009495532330547 1103 30-31 0.42144399692146806 1103 32-33 0.4118992882534087 1103 34-35 0.4895978186344436 1103 36-37 0.5125874114180675 1103 38-39 0.5157141289947731 1103 40-41 0.40572399199268006 1103 42-43 0.5243850788287787 1103 44-45 0.5351681036124987 1103 46-47 0.514831195881456 1103 48-49 0.4946031462872966 1103 50-51 0.4961787181715849 1103 52-53 0.5491186296428268 1103 54-55 0.6450276628756555 1103 56-57 0.651669066566626 1103 58-59 0.6149457180610725 1103 60-61 0.5932884881838518 1103 62-63 0.6619354072897679 1103 64-65 0.6735064313177404 1103 66-67 0.6942799365314514 1103 68-69 0.6234931182217238 1103 70-71 0.5359919716587811 1103 72-73 0.5877173954921986 1103 74-75 0.5151486062642121 1103 76-77 0.4957113379668314 1103 78-79 0.5366558450778349 1103 80-81 0.4686312687141019 1103 82-83 0.43690319342093176 1103 84-85 0.5108646697852741 1103 86-87 0.47239174323329536 1103 88-89 0.4393981644223679 1103 90-91 0.47916675340609416 1103 92-93 0.47425644888486573 1103 94-95 0.488005347459044 1103 96-97 0.5171052330166646 1103 98-99 0.4722941729836947 1103 100 0.57296146505076 1104 1 0.2493947006219699 1104 2 0.221620925439872 1104 3 0.2305185049146985 1104 4 0.1450044980405707 1104 5 0.15989688796000934 1104 6 0.18023348598138966 1104 7 0.1626459964055229 1104 8 0.18815664540028365 1104 9 0.12523874250984335 1104 10-11 0.23010923400877203 1104 12-13 0.22611967634674102 1104 14-15 0.37994240894632725 1104 16-17 0.35368770776720737 1104 18-19 0.3482393528915253 1104 20-21 0.33514564431256844 1104 22-23 0.3123681598043646 1104 24-25 0.3330504870965285 1104 26-27 0.3539370671391282 1104 28-29 0.34286857551861516 1104 30-31 0.35711795408589353 1104 32-33 0.46077454883153734 1104 34-35 0.44462420956825355 1104 36-37 0.40218763254469536 1104 38-39 0.4479109581182925 1104 40-41 0.5331135195327477 1104 42-43 0.4245554388312911 1104 44-45 0.5547974962741336 1104 46-47 0.6176594802670294 1104 48-49 0.6567127471251126 1104 50-51 0.6417181382868407 1104 52-53 0.5946907562356145 1104 54-55 0.6464994543647009 1104 56-57 0.6704455579875059 1104 58-59 0.6386496550620038 1104 60-61 0.7011956370269559 1104 62-63 0.7164104132639224 1104 64-65 0.6757870749195476 1104 66-67 0.6254614332547348 1104 68-69 0.6534525820116883 1104 70-71 0.6072739224184645 1104 72-73 0.5768614878984621 1104 74-75 0.5450711789094882 1104 76-77 0.520481814479286 1104 78-79 0.6155450802418194 1104 80-81 0.5970399829530919 1104 82-83 0.6036010615532561 1104 84-85 0.6430302240279211 1104 86-87 0.6665674574073179 1104 88-89 0.6573995013133995 1104 90-91 0.6687503459868971 1104 92-93 0.7109401221857006 1104 94-95 0.7420675029811008 1104 96-97 0.8450438329371543 1104 98-99 0.8668262446031711 1104 100 0.8439054488759226 1105 1 0.215117716148562 1105 2 0.14994382652118077 1105 3 0.1796556005192329 1105 4 0.14771087765925017 1105 5 0.16557212722887016 1105 6 0.1685011556692686 1105 7 0.13134517513482535 1105 8 0.15431639269736763 1105 9 0.22341719516956005 1105 10-11 0.21920094768057652 1105 12-13 0.1787199649748672 1105 14-15 0.2915430686086964 1105 16-17 0.2917460032076207 1105 18-19 0.23540449620480075 1105 20-21 0.2846944776906284 1105 22-23 0.23250012673030085 1105 24-25 0.20064831077567646 1105 26-27 0.2241129242816413 1105 28-29 0.2749138251605814 1105 30-31 0.2692863960555556 1105 32-33 0.27950211495365807 1105 34-35 0.2910845359474834 1105 36-37 0.258920836182547 1105 38-39 0.2030717964696649 1105 40-41 0.24095891297201888 1105 42-43 0.29069270703392647 1105 44-45 0.2933240803440498 1105 46-47 0.3361705408427582 1105 48-49 0.3618558433559613 1105 50-51 0.3186879012348882 1105 52-53 0.2794101051721398 1105 54-55 0.2613435631366485 1105 56-57 0.32623698230771936 1105 58-59 0.30065647148135355 1105 60-61 0.28192536872130347 1105 62-63 0.29135905585390987 1105 64-65 0.32037474631618323 1105 66-67 0.2593391511150642 1105 68-69 0.2928370234466726 1105 70-71 0.30207980467210405 1105 72-73 0.3311652784766679 1105 74-75 0.3047208598786 1105 76-77 0.2807341591091266 1105 78-79 0.38924397130760724 1105 80-81 0.3931510479385203 1105 82-83 0.3890116124994982 1105 84-85 0.4357669099445012 1105 86-87 0.42412182839402135 1105 88-89 0.3802460988053191 1105 90-91 0.37017524496877385 1105 92-93 0.38337277705804595 1105 94-95 0.36863028235490347 1105 96-97 0.3088500276588242 1105 98-99 0.4053362062009569 1105 100 0.4005281001550607 1106 1 0.1518815880519 1106 2 0.16620062659721668 1106 3 0.1524000334732989 1106 4 0.10257996183700158 1106 5 0.13370437301161076 1106 6 0.13484572810239115 1106 7 0.1161860173214464 1106 8 0.11861862920877542 1106 9 0.14998480820833038 1106 10-11 0.18325166871050413 1106 12-13 0.21266495634868932 1106 14-15 0.21542218508189848 1106 16-17 0.21076160585343473 1106 18-19 0.19609579242217734 1106 20-21 0.2547654257013221 1106 22-23 0.25075444815776393 1106 24-25 0.22755518018212229 1106 26-27 0.2732162237543747 1106 28-29 0.2901182696078237 1106 30-31 0.25332107951173555 1106 32-33 0.2673802457580763 1106 34-35 0.27872326756925503 1106 36-37 0.3376789501689075 1106 38-39 0.33731884560015146 1106 40-41 0.25466628641649436 1106 42-43 0.3212038449023069 1106 44-45 0.20102507400643788 1106 46-47 0.06845364807861642 1106 48-49 0.13138479942194436 1106 50-51 0.19364190650563273 1106 52-53 0.17928991778177306 1106 54-55 0.168949357308108 1106 56-57 0.056355489151698634 1106 58-59 0.11977262367214081 1106 60-61 0.1235051389711046 1106 62-63 0.10064558523608724 1106 64-65 0.10968086948449951 1106 66-67 0.06023695312403987 1106 68-69 0.07529243389337381 1106 70-71 0.12281277466333762 1106 72-73 0.10847969611699426 1106 74-75 0.08045292036064922 1106 76-77 0.021388307068960444 1106 78-79 0.11290965209569137 1106 80-81 0.10703462698619859 1106 82-83 0.08764703401137552 1106 84-85 0.08987298200406357 1106 86-87 0.11523545158279447 1106 88-89 0.0732579211659008 1106 90-91 0.03857172047906232 1106 92-93 0.10130738555053398 1106 94-95 0.07514705729197857 1106 96-97 0.08434574163612396 1106 98-99 0.08783913771585716 1106 100 0.08093015986291974 1107 1 0.17812526496502556 1107 2 0.1618935996151052 1107 3 0.15288739981276933 1107 4 0.07255867932749283 1107 5 0.06465205293357457 1107 6 0.08955096767714821 1107 7 0.06503763714312072 1107 8 0.06024643160211696 1107 9 0.1104502315527256 1107 10-11 0.15451787561861607 1107 12-13 0.09663498045612329 1107 14-15 0.23250225924751788 1107 16-17 0.22927745405414157 1107 18-19 0.23594150263924263 1107 20-21 0.2591562676527914 1107 22-23 0.23349983817213626 1107 24-25 0.24182172748525232 1107 26-27 0.29082304844137497 1107 28-29 0.3023495887710368 1107 30-31 0.2642529420143447 1107 32-33 0.3313152782962874 1107 34-35 0.292584655081221 1107 36-37 0.274036960144187 1107 38-39 0.25230214959923103 1107 40-41 0.292223493822128 1107 42-43 0.29257442210590057 1107 44-45 0.32476781257557263 1107 46-47 0.33305567660156044 1107 48-49 0.23973341373915957 1107 50-51 0.20957188582753616 1107 52-53 0.14154955696069038 1107 54-55 0.1942741978634146 1107 56-57 0.22882026782912135 1107 58-59 0.16474845928524928 1107 60-61 0.1536231333308251 1107 62-63 0.2014956962702641 1107 64-65 0.14946042785162206 1107 66-67 0.12514416552610186 1107 68-69 0.1339151025017884 1107 70-71 0.08438586890128619 1107 72-73 0.08951788735218003 1107 74-75 0.10608902728281322 1107 76-77 0.09985093807633305 1107 78-79 0.17327034509438377 1107 80-81 0.149576036155473 1107 82-83 0.19992519806925912 1107 84-85 0.18698034148369302 1107 86-87 0.24293033156831711 1107 88-89 0.25957728476710074 1107 90-91 0.2524949244821606 1107 92-93 0.2781765375750105 1107 94-95 0.21687653255537498 1107 96-97 0.28357894091075764 1107 98-99 0.2873688914692494 1107 100 0.29438233259187996 1108 1 0.07934725980921797 1108 2 0.0842484174779159 1108 3 0.0994681702104856 1108 4 0.06600621403568141 1108 5 0.10623798927047545 1108 6 0.101590456522473 1108 7 0.1182676513197336 1108 8 0.08694505266253572 1108 9 0.0756253898164374 1108 10-11 0.10670267038562287 1108 12-13 0.12113254552438235 1108 14-15 0.14168130263765732 1108 16-17 0.16166506739247666 1108 18-19 0.1578328629968908 1108 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-0.8437931567470258 2316 72-73 -0.8392450225377814 2316 74-75 -0.8524979485345874 2316 76-77 -0.9391609579557532 2316 78-79 -0.9575579865579336 2316 80-81 -0.8826537203024536 2316 82-83 -0.9074023812811802 2316 84-85 -0.8507935097965991 2316 86-87 -0.889462874104197 2316 88-89 -0.8930292720712174 2316 90-91 -0.9169669603135304 2316 92-93 -0.9688640562509825 2316 94-95 -0.9535525321743563 2316 96-97 -0.9886814073645986 2316 98-99 -1.040232188839859 2316 100 -1.0594927688987639 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 19.0 4 30.0 5 75.0 6 243.0 7 664.0 8 1537.0 9 2972.0 10 5475.0 11 10923.0 12 19859.0 13 28226.0 14 30813.0 15 38281.0 16 50943.0 17 67788.0 18 67158.0 19 41711.0 20 28319.0 21 24581.0 22 26186.0 23 29554.0 24 31523.0 25 31873.0 26 35804.0 27 43612.0 28 53503.0 29 66524.0 30 82962.0 31 101693.0 32 129520.0 33 168201.0 34 242089.0 35 399032.0 36 801361.0 37 1662289.0 38 1510745.0 39 240768.0 40 153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.47918954920427 17.921746082580878 13.757105038212261 22.841959330002588 2 14.342440258695838 23.311682057976906 35.70598495468214 26.63989272864512 3 20.1664256739445 28.5162269804229 29.412055099378026 21.905292246254575 4 13.906602249128685 18.991923673116098 33.215309477342515 33.886164600412705 5 13.15693294627405 37.783385138523 32.400801522741816 16.658880392461132 6 30.859566037301178 32.96206035194882 18.23582940322724 17.942544207522765 7 24.640103208480472 33.412988602966585 21.883423125150156 20.063485063402787 8 28.852463872095203 30.572978203132458 21.45210975116093 19.12244817361141 9 29.02176630004165 14.674682073924098 19.248233040089268 37.05531858594499 10-11 24.910120420864175 26.818276062938747 24.796808304070087 23.474795212127 12-13 27.916988918910913 24.671557770091262 25.68098344254256 21.730469868455263 14-15 26.957571309622153 23.037936356964433 25.25985598204383 24.744636351369586 16-17 23.187228279650224 26.554404955060214 28.095703157139518 22.162663608150048 18-19 25.186003995379313 25.377495877913848 29.390169852987153 20.046330273719683 20-21 24.56921727950702 25.32947023695364 28.55514584069203 21.54616664284731 22-23 22.845699313829353 27.038970767070257 25.210501580242866 24.904828338857516 24-25 23.202699697268123 27.830507668513654 24.52817706996716 24.438615564251062 26-27 24.41406545707321 25.285746870089714 25.512973518447694 24.78721415438938 28-29 23.847612731658995 24.193591384967796 29.007967983254403 22.95082790011881 30-31 26.055550038125126 24.675755424221514 27.424039176517063 21.8446553611363 32-33 21.26298798377519 26.512880732785497 28.670760771957 23.553370511482317 34-35 20.825162025978464 27.418129630327503 28.55726898163774 23.19943936205629 36-37 23.96161276415085 27.270603025825157 26.40128714402783 22.366497065996164 38-39 24.988538790393978 25.082359445912633 26.16483078877476 23.764270974918627 40-41 23.07693257062064 26.85634762163509 26.13681205534527 23.929907752399 42-43 26.09920788247119 25.471377069402106 26.075157696158357 22.354257351968347 44-45 25.795769935963868 24.723558890891745 27.07183552354645 22.408835649597936 46-47 23.65188921378079 25.639376898688194 28.915096908897358 21.793636978633653 48-49 24.67813826657602 26.477569050063686 27.879316752591084 20.96497593076921 50-51 23.002955360553738 27.48832530144571 26.052302616611843 23.45641672138871 52-53 22.23912672491068 28.251573778115755 23.788516278542392 25.72078321843117 54-55 23.484654378979776 27.11334802853139 23.612492728509917 25.789504863978912 56-57 24.378864492237334 24.88706458788749 24.499534135509656 26.23453678436552 58-59 23.914401449958135 24.62432136075526 27.189117907847017 24.27215928143959 60-61 24.421025310292052 25.65462256012473 25.876097799344404 24.048254330238812 62-63 20.828380883978884 26.678431854022584 27.096149218958555 25.39703804303998 64-65 20.685176067439915 26.72018484497627 26.518387079150536 26.076252008433283 66-67 23.310244924617376 26.528135665848435 24.871057446595987 25.290561962938202 68-69 24.010108902892284 25.006863973367206 24.968662680776323 26.014364442964187 70-71 22.466529647754985 25.996672711296 25.352804499573807 26.18399314137521 72-73 24.27360375376653 25.06195199084794 25.44854821131162 25.21589604407391 74-75 24.367048067346367 24.286835032584232 26.051719265074503 25.2943976349949 76-77 22.216834856157874 25.74340852150947 27.477824081882847 24.561932540449813 78-79 22.623810517822825 25.547213431812978 27.240531350378276 24.588444699985924 80-81 21.625055527731696 25.964368289619156 26.347218577968423 26.063357604680725 82-83 21.33591489725127 26.74145928627031 24.39945686449085 27.52316895198757 84-85 21.815750355821844 26.049647699759266 24.43428481907432 27.70031712534457 86-87 22.254042721948604 24.17372473453053 25.351721334809945 28.220511208710917 88-89 22.177605674408685 23.67164019952055 26.870414654149897 27.28033947192087 90-91 22.78927009885913 24.45197084308564 26.007702344939233 26.751056713115993 92-93 20.65128513563881 25.311787012803222 26.57290580773196 27.46402204382601 94-95 19.966211885524228 25.56223155908296 26.853907420270133 27.617649135122672 96-97 21.658402878847355 25.003959591677855 25.613057922922856 27.724579606551934 98-99 22.158270007339123 24.110781271083525 25.53748383253634 28.19346488904101 100 21.795993956756348 25.056883734041328 25.981553987752577 27.165568321449747 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 286.0 1 211.5 2 189.0 3 249.0 4 225.0 5 263.5 6 447.5 7 841.5 8 1334.5 9 1461.5 10 1231.5 11 1161.0 12 1284.5 13 1467.5 14 1771.0 15 2131.5 16 2546.0 17 3023.0 18 3526.5 19 4115.0 20 4845.5 21 5711.0 22 6983.5 23 8557.0 24 10209.0 25 12726.5 26 16366.5 27 20748.5 28 25741.0 29 32884.0 30 41377.5 31 49350.5 32 58505.5 33 68126.5 34 77407.0 35 84941.5 36 91552.5 37 99854.5 38 104698.0 39 104845.0 40 102954.0 41 101644.0 42 103368.5 43 112081.0 44 125309.5 45 137552.5 46 159607.0 47 199102.0 48 322880.5 49 468723.0 50 643855.5 51 628039.0 52 379555.5 53 251295.5 54 205150.0 55 170380.0 56 148768.0 57 132482.0 58 124823.5 59 115422.5 60 98053.0 61 81661.0 62 67746.0 63 53140.5 64 39311.0 65 31245.5 66 24116.5 67 17829.0 68 13932.5 69 11406.5 70 9739.0 71 8473.0 72 7442.0 73 7421.0 74 5830.0 75 4360.0 76 3233.0 77 2036.0 78 1412.0 79 833.5 80 568.0 81 396.0 82 274.5 83 193.0 84 122.0 85 78.0 86 41.5 87 24.0 88 18.5 89 12.5 90 8.5 91 4.5 92 2.5 93 3.0 94 2.5 95 1.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011485892756647173 2 0.04451194829044499 3 0.0012012466636608075 4 0.0 5 0.001431622736143702 6 0.0 7 0.0 8 0.0 9 9.379597236803566E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.8543529558895646E-4 22-23 0.0017442759773704875 24-25 0.0031347601291422437 26-27 0.008030251669403753 28-29 0.003291086749755637 30-31 0.005241055648985852 32-33 0.0025176813635630624 34-35 0.006104965920796707 36-37 0.0 38-39 0.0 40-41 0.0018759194473607131 42-43 0.0023695824598240585 44-45 0.002270849857331389 46-47 0.002089840086094829 48-49 0.001160108079288862 50-51 0.0015879493567570947 52-53 0.006598628933260052 54-55 0.003373363918499528 56-57 0.028887513945980103 58-59 0.016167463658174563 60-61 0.01927754063669364 62-63 0.014842801241397922 64-65 0.0023695824598240585 66-67 0.0014645336036412584 68-69 8.227716874389092E-6 70-71 0.0 72-73 0.002254394423582611 74-75 0.0012752961155303093 76-77 7.404945186950183E-4 78-79 0.002690463417925233 80-81 0.0016702265255009855 82-83 0.0013740287180229783 84-85 0.003998670400953099 86-87 8.227716874389092E-4 88-89 8.556825549364656E-4 90-91 6.499896330767383E-4 92-93 5.759401812072364E-4 94-95 5.101184462121237E-4 96-97 7.404945186950183E-5 98-99 0.0 100 0.002484770496065506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6077020.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.495262093354555 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7999785304573 26.394259338283792 2 11.391006003504346 7.630894631887066 3 3.5114018477792173 3.52845975619363 4 1.6741462775413007 2.2430387339543905 5 0.9631368691762163 1.6130260932415152 6 0.651784492214427 1.3099015437063746 7 0.45601888218724584 1.0692130384866263 8 0.3345457679949441 0.8964558544970592 9 0.2557581283013953 0.7710016985964934 >10 1.6417615250015252 10.42877074027754 >50 0.15643327749878433 3.6661639505744685 >100 0.13617175885492058 9.285541801573615 >500 0.015117927380345954 3.4508147219264327 >1k 0.009913394636546686 6.2363469594743 >5k 9.417724904719322E-4 2.35584076715225 >10k+ 0.0018835449809438643 19.120270370174534 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 135657 2.2322947760580023 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 113680 1.8706537085611041 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 74904 1.232577809518481 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 61051 1.0046206857966569 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 56689 0.9328420837844865 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 50460 0.8303411869633471 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 50351 0.8285475446847302 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 47961 0.7892190580251505 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 46539 0.7658194312343879 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 43200 0.7108747379472176 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 39562 0.6510098699691625 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 38823 0.6388493044288154 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 23991 0.39478231106693745 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 21924 0.36076892950821293 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 20862 0.3432932588670105 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 20542 0.33802752006740144 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 19600 0.32252650147605244 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 19446 0.3199923646787406 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17400 0.2863245472287404 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 17080 0.2810588084291314 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 16206 0.26667675933269924 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 15669 0.2578401914096054 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 14981 0.24651885299044599 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 14726 0.24232271738450756 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 14634 0.24080881747961994 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 14330 0.23580636561999138 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 14167 0.23312412991894055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13579 0.22344833487465895 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 12986 0.21369026266163352 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 12120 0.1994398570351916 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 11942 0.19651078982790907 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 11598 0.19085012061832937 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 11507 0.18935267614719056 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 11229 0.18477806556503024 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 11036 0.18160216685151603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10661 0.1754313791957242 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 10403 0.17118587728853946 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 10148 0.16698974168260103 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 9949 0.16371511036659417 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 9631 0.15848228243448267 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 9434 0.15524056198597339 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 8871 0.1459761527854113 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 8637 0.1421255812881972 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 8181 0.13462190349875433 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 7967 0.1311004406765158 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 7884 0.1297346396753672 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA 7810 0.12851693757795762 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 7782 0.12805618543299183 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 7728 0.1271675920105578 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 7399 0.12175375430720978 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 6844 0.11262098857663788 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 6700 0.11025140611681382 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 6097 0.10032877956630058 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.007059381078225841 0.0 0.0 1.6455433748778184E-5 3.291086749755637E-5 2 0.010564388466715594 0.0 0.0 3.291086749755637E-5 3.291086749755637E-5 3 0.011123873214174053 0.0 0.0 3.291086749755637E-5 3.291086749755637E-5 4 0.013328901336510329 0.0 0.0 4.9366301246334553E-5 3.291086749755637E-5 5 0.05346370424978032 0.0 0.0 2.7974237372922913E-4 3.291086749755637E-5 6 0.11647156007385198 0.0 0.0 5.594847474584583E-4 3.291086749755637E-5 7 0.13205485583394494 0.0 0.0 6.746727836999055E-4 3.291086749755637E-5 8 0.1538747609848248 0.0 0.0 7.569499524437965E-4 3.291086749755637E-5 9 0.16929350240742996 1.6455433748778184E-5 0.0 0.0010366923261730255 3.291086749755637E-5 10-11 0.32106196787241115 1.6455433748778184E-5 0.0 0.001892374881109491 3.291086749755637E-5 12-13 0.5312143122780573 1.6455433748778184E-5 0.0 0.004286640491556717 4.113858437194546E-5 14-15 0.7606688804710203 1.6455433748778184E-5 0.0 0.006730272403250278 9.87326024926691E-5 16-17 0.8540781501459598 1.6455433748778184E-5 0.0 0.007709370711302579 1.234157531158364E-4 18-19 0.8941471313242346 1.6455433748778184E-5 0.0 0.008309994043132983 1.4809890373900365E-4 20-21 1.036618276721156 1.6455433748778184E-5 0.0 0.010909952575439936 1.4809890373900365E-4 22-23 1.077765088809976 1.6455433748778184E-5 0.0 0.014538375717045526 1.7278205436217092E-4 24-25 1.2640652819967682 1.6455433748778184E-5 0.0 0.029200167187206888 2.139206387341164E-4 26-27 1.6644506682551645 1.6455433748778184E-5 0.0 0.07478171867132247 2.139206387341164E-4 28-29 1.7627142908859934 1.6455433748778184E-5 0.0 0.1271758197274322 2.139206387341164E-4 30-31 1.7976574044515239 1.6455433748778184E-5 0.0 0.2360696525599718 2.139206387341164E-4 32-33 1.8290461443273185 1.6455433748778184E-5 0.0 0.3947329447656911 2.2214835560850548E-4 34-35 1.852083751575608 2.4683150623167277E-5 0.0 0.5918608133591794 2.468315062316728E-4 36-37 1.892350197958868 3.291086749755637E-5 0.0 0.8802176066558938 2.468315062316728E-4 38-39 1.9327236046614953 3.291086749755637E-5 0.0 1.3186068171570935 2.5505922310606186E-4 40-41 1.9689749252100537 3.291086749755637E-5 0.0 2.004806632198018 2.7974237372922913E-4 42-43 2.049277441904091 4.9366301246334553E-5 0.0 2.4728238511638927 2.879700906036182E-4 44-45 2.1386469025937056 4.9366301246334553E-5 0.0 2.920954678444369 3.044255243523964E-4 46-47 2.1715989086756338 4.9366301246334553E-5 0.0 3.4423615522081548 3.2910867497556366E-4 48-49 2.1938548828208564 4.9366301246334553E-5 0.0 3.902801043932717 3.9493040997067643E-4 50-51 2.233849814547262 4.9366301246334553E-5 0.0 4.431909060690931 3.9493040997067643E-4 52-53 2.246693280588183 4.9366301246334553E-5 0.0 5.119746191389859 4.5252442809140005E-4 54-55 2.290086259383711 4.9366301246334553E-5 0.0 5.679716374143906 4.936630124633455E-4 56-57 2.3762222273416906 4.9366301246334553E-5 0.0 6.2408960312784885 4.936630124633455E-4 58-59 2.4036698908346525 4.9366301246334553E-5 0.0 6.81898364658994 5.183461630865129E-4 60-61 2.424428420508736 4.9366301246334553E-5 0.0 7.319648446113391 5.430293137096801E-4 62-63 2.4408015770887705 4.9366301246334553E-5 0.0 7.839780023761646 5.923956149560146E-4 64-65 2.453505171942827 4.9366301246334553E-5 0.0 8.41273354374348 6.088510487047928E-4 66-67 2.4726510691095305 4.9366301246334553E-5 0.0 8.979672602690133 6.088510487047928E-4 68-69 2.4934507373679864 4.9366301246334553E-5 0.0 9.679012081579458 6.088510487047928E-4 70-71 2.519499688992302 4.9366301246334553E-5 0.0 10.568230810495933 6.088510487047928E-4 72-73 2.5853378794211634 4.9366301246334553E-5 0.0 11.247930729206091 6.499896330767383E-4 74-75 2.6505013970663254 4.9366301246334553E-5 0.0 11.89051541709588 6.582173499511273E-4 76-77 2.6703631056011004 4.9366301246334553E-5 0.0 12.574995639310057 6.582173499511273E-4 78-79 2.684564144926296 4.9366301246334553E-5 0.0 13.208176046812419 6.582173499511273E-4 80-81 2.714373163162208 4.9366301246334553E-5 0.0 13.939636532379357 6.993559343230728E-4 82-83 2.724970462496421 4.9366301246334553E-5 0.0 14.856681399765016 7.651776693181856E-4 84-85 2.765714116458396 4.9366301246334553E-5 0.0 15.66545774080059 7.734053861925747E-4 86-87 2.8358636305294374 4.9366301246334553E-5 0.0 16.433671437645426 8.06316253690131E-4 88 2.852862093591925 4.9366301246334553E-5 0.0 17.04916225386785 8.392271211876874E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 3290 0.0 36.586758 1 TCCGATC 2605 0.0 36.442272 3 CGATCTC 2815 0.0 30.550634 5 CCGATCT 3265 0.0 29.3626 4 ATCTACA 4365 0.0 28.542227 2 TCTACAC 5000 0.0 27.351728 3 TCTCGCG 1335 0.0 26.401434 8 AGTACTC 3250 0.0 26.17235 5 TACACTC 5065 0.0 25.979221 5 CGATACA 500 0.0 23.50985 1 CTACACT 6180 0.0 23.421234 4 ATCTCGC 3050 0.0 21.57127 7 AAAGTAC 3755 0.0 21.526842 3 GATGTAA 2295 0.0 21.51228 1 CTCGCGC 1640 0.0 21.491411 9 TTCCGAT 4590 0.0 21.202345 2 AAGTACT 4140 0.0 20.545927 4 TATCACG 350 3.6379788E-12 20.148811 2 CACTCTT 6635 0.0 20.115227 7 ATGTAAG 2875 0.0 19.296137 2 >>END_MODULE