FastQCFastQC Report
Fri 27 May 2016
ERR765506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16746416
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1870151.1167464130832532No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1664380.9938723605098547No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG734320.4384938245890942No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG524370.3131237155460607No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC508750.3037963466332139No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC489210.2921281783517142No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG487010.2908144644203273No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT472300.28203049536091784No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC352450.2104629432351376No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA339820.2029210309835848No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC328160.19595834714723437No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT296710.17717820935536296No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG253400.15131595918792415No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC198210.11835965379099624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192470.11493205471546866No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG185440.11073414156199154No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT179580.10723488536293377No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA175200.10461940035408172No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG62650.021.6050031
GTATAAG63700.021.2488771
CTAACGC29350.020.816383
TAGGCAT80550.020.770925
TAGTACT74950.020.3163014
CTAGGCA91800.019.7101214
GTACATA42300.019.665991
CATGGGG401050.019.2300914
ATGGGTA109000.019.2300155
TGATCGC45150.019.1528179
CTATTGA86300.018.6790969
TCTAACG33550.018.6308632
GGGCTAT93450.018.6077426
GGGTACC98300.018.5502917
TACGGGC42300.018.4433164
GTGATCG47200.018.4205368
TTTAGGG94600.018.2326092
CATGGGT145400.018.0037124
ATACGGG43650.017.9805183
AGGGCTA93650.017.6646965
Produced by FastQC (version 0.11.5)