##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765506_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16746416
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15632222440909	33.0	33.0	33.0	33.0	33.0
2	32.116372183755615	33.0	33.0	33.0	33.0	33.0
3	32.13571889053753	33.0	33.0	33.0	33.0	33.0
4	35.93065459498916	37.0	37.0	37.0	33.0	37.0
5	35.977171175014405	37.0	37.0	37.0	33.0	37.0
6	35.94050183633322	37.0	37.0	37.0	33.0	37.0
7	35.894040790578714	37.0	37.0	37.0	33.0	37.0
8	35.873231143905656	37.0	37.0	37.0	33.0	37.0
9	35.888458163227284	37.0	37.0	37.0	33.0	37.0
10-11	35.87553978714013	37.0	37.0	37.0	33.0	37.0
12-13	35.85193527379232	37.0	37.0	37.0	33.0	37.0
14-15	37.75699887068373	40.0	37.0	40.0	33.0	40.0
16-17	37.7780306245826	40.0	37.0	40.0	33.0	40.0
18-19	37.81258330140611	40.0	37.0	40.0	33.0	40.0
20-21	37.8352281168699	40.0	37.0	40.0	33.0	40.0
22-23	37.84327207684319	40.0	37.0	40.0	33.0	40.0
24-25	37.863614011499536	40.0	37.0	40.0	33.0	40.0
26-27	37.84335060110772	40.0	37.0	40.0	33.0	40.0
28-29	37.79922071086733	40.0	37.0	40.0	33.0	40.0
30-31	37.74054463355024	40.0	37.0	40.0	33.0	40.0
32-33	37.63744167707287	40.0	37.0	40.0	33.0	40.0
34-35	37.55730178325918	40.0	37.0	40.0	33.0	40.0
36-37	37.4848708822234	40.0	37.0	40.0	33.0	40.0
38-39	37.352903003245586	40.0	37.0	40.0	33.0	40.0
40-41	37.25349519562872	40.0	37.0	40.0	33.0	40.0
42-43	37.108020993865196	40.0	37.0	40.0	33.0	40.0
44-45	36.92511693248275	40.0	37.0	40.0	33.0	40.0
46-47	36.72546501890315	37.0	37.0	40.0	33.0	40.0
48-49	36.54890882323716	37.0	37.0	40.0	33.0	40.0
50-51	36.374806257052256	37.0	37.0	40.0	33.0	40.0
52-53	36.22549102446756	37.0	37.0	40.0	33.0	40.0
54-55	36.0809654137339	37.0	37.0	40.0	33.0	40.0
56-57	35.91123826136888	37.0	37.0	40.0	33.0	40.0
58-59	35.734334140511024	37.0	37.0	40.0	27.0	40.0
60-61	35.52884533622	37.0	37.0	40.0	27.0	40.0
62-63	35.28549141499889	37.0	33.0	40.0	27.0	40.0
64-65	35.1039322682537	37.0	33.0	37.0	27.0	40.0
66-67	34.90370333568687	37.0	33.0	37.0	27.0	40.0
68-69	34.69673937993658	37.0	33.0	37.0	27.0	40.0
70-71	34.4400555617393	37.0	33.0	37.0	27.0	40.0
72-73	34.222539288406544	37.0	33.0	37.0	27.0	38.5
74-75	34.00958369241514	37.0	33.0	37.0	27.0	37.0
76-77	33.8046512758312	37.0	33.0	37.0	27.0	37.0
78-79	33.63900574307959	37.0	33.0	37.0	27.0	37.0
80-81	33.509485522155906	37.0	33.0	37.0	27.0	37.0
82-83	33.39176690104915	37.0	33.0	37.0	27.0	37.0
84-85	33.257946595856694	37.0	33.0	37.0	27.0	37.0
86-87	33.14892837368903	37.0	33.0	37.0	27.0	37.0
88-89	33.05048136867017	37.0	33.0	37.0	27.0	37.0
90-91	32.945598120815816	37.0	33.0	37.0	27.0	37.0
92-93	32.81595512735382	37.0	33.0	37.0	27.0	37.0
94-95	32.75904584598878	37.0	33.0	37.0	27.0	37.0
96-97	32.72579610467099	37.0	33.0	37.0	27.0	37.0
98-99	32.61623269122181	37.0	33.0	37.0	22.0	37.0
100	32.50849047342428	37.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	215.0
8	1707.0
9	3640.0
10	4386.0
11	6183.0
12	9929.0
13	16888.0
14	27281.0
15	33286.0
16	36376.0
17	42697.0
18	50815.0
19	60064.0
20	70673.0
21	85574.0
22	105725.0
23	111021.0
24	105924.0
25	105738.0
26	114055.0
27	129702.0
28	156991.0
29	197057.0
30	255335.0
31	333890.0
32	454578.0
33	633998.0
34	898608.0
35	1374071.0
36	2611831.0
37	5417757.0
38	3284530.0
39	5886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.123533381446684	18.536312233500997	12.1953840346703	25.144770350382018
2	15.534849286382535	21.02163839564054	37.79142935300861	25.652082964968315
3	18.2126253163662	26.893617117835838	29.270418219635776	25.62333934616219
4	12.65388964420805	17.515061133080653	36.88088245269913	32.95016677001216
5	13.467191687305993	37.97537023814147	34.05214554384749	14.505292530705043
6	31.397124017461408	36.49242918604196	18.0057870292963	14.104659767200337
7	26.924215169195648	31.89207933018727	22.922778921239964	18.260926579377124
8	27.175916327409993	32.92054849228635	20.63989094741227	19.263644232891384
9	27.626687405830598	14.452047530647752	19.713728597211485	38.20753646631017
10-11	25.152013234667685	25.68468543972478	27.19317194525377	21.97012938035377
12-13	26.500178500885756	23.46506928602124	26.810241869371986	23.22451034372102
14-15	24.752290281096563	24.12138513697498	24.459254445846803	26.667070136081655
16-17	23.73716859774653	26.440203682985064	26.458258292401194	23.364369426867217
18-19	24.39605286289317	25.89030691701436	27.639457302386372	22.074182917706093
20-21	24.698260809954796	25.476131728723328	26.68664148794584	23.138965973376035
22-23	23.710256042244225	25.49702722829043	25.57561800214203	25.217098727323307
24-25	23.70591930954098	26.316632084677394	25.507695987977684	24.469752617803938
26-27	24.49118013318446	25.208262696252653	25.699828106318744	24.600729064244145
28-29	23.770306095235387	25.217857169729495	26.7819088616309	24.229927873404215
30-31	24.882010574680578	25.211931914267506	26.25282627638057	23.653231234671345
32-33	23.146641881229048	25.72856810802685	26.201320672497076	24.923469338247028
34-35	23.4370524221135	25.92841966449241	26.919600627884687	23.714927285509404
36-37	24.698799605336287	25.42328626991016	26.082917239152586	23.794996885600966
38-39	25.016842435892155	24.82428451743928	25.916928962003155	24.241944084665413
40-41	24.008606380106404	25.654836481853266	26.319522087520415	24.017035050519922
42-43	25.32044468500006	25.10465821462933	25.82714414833598	23.74775295203463
44-45	24.928396619312455	25.216703084409225	25.72198433384194	24.13291596243638
46-47	23.974903047911862	25.148670617044267	27.079310582037376	23.797115753006494
48-49	25.07472147395915	25.497387764022744	26.160081785767392	23.267808976250716
50-51	23.608071806504803	26.123093299561972	25.84997388682827	24.418861007104955
52-53	23.352282568876042	26.16342639984122	24.964876079512084	25.519414951770653
54-55	23.98132746564944	26.068762361041525	24.956536218543977	24.99337395476506
56-57	24.567646035404508	24.853910403635865	24.948246032096268	25.63019752886336
58-59	24.09953067023398	24.92061068852718	26.168383716712317	24.81147492452652
60-61	24.326720339504796	25.54306859800984	25.560869573785165	24.569341488700204
62-63	23.069138261414846	25.79277361770742	25.745338723719197	25.392749397158543
64-65	22.874012447246223	25.699741087437538	26.044676181346045	25.381570283970195
66-67	24.420001030072903	25.302039021848742	25.480468548813885	24.79749139926447
68-69	24.78418367249446	24.93125991854018	25.1199301390817	25.164626269883655
70-71	23.533617937879107	25.36019320457308	25.657200420066538	25.448988437481272
72-73	24.9378645789838	25.079003340770704	25.46565326382379	24.517478816421708
74-75	25.102273030608547	24.89385431423638	25.42191488077149	24.58195777438359
76-77	24.013514552930378	25.335394481908896	26.39441048610731	24.256680479053415
78-79	24.3640888760856	25.42715003143793	26.07582413559734	24.13293695687913
80-81	23.39149015989385	25.58231126028998	26.252669630165414	24.773528949650757
82-83	23.576314368812486	25.845743826263025	25.120057264062744	25.45788454086175
84-85	23.799450927102434	25.899083879263202	24.768342329884916	25.533122863749448
86-87	23.79146446244018	25.044234203900924	25.267619882000126	25.896681451658772
88-89	23.989636697706153	24.62825276312976	25.84000586892711	25.54210467023697
90-91	24.35530199742416	25.00822864993771	25.50208797513586	25.134381377502272
92-93	23.469587499860413	25.44561906969131	25.559865210872108	25.52492821957617
94-95	22.621999330353024	25.634649352265544	26.15563816299843	25.587713154383007
96-97	24.02634666032896	25.209304005921297	25.39557461378863	25.368774719961113
98-99	23.8983762256951	25.10143065835699	25.230664280643687	25.76952883530422
100	23.420287369548344	25.545677651141073	25.382393255752543	25.65164172355804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	754.0
1	669.5
2	863.5
3	1135.5
4	935.0
5	847.5
6	1165.0
7	2324.0
8	3702.5
9	3531.0
10	2312.0
11	1771.5
12	1963.5
13	2244.0
14	2709.5
15	3346.5
16	4075.0
17	5019.5
18	6078.5
19	7243.5
20	8901.0
21	10964.0
22	13837.5
23	17734.5
24	22349.0
25	28232.5
26	38031.5
27	50444.5
28	62298.5
29	82294.5
30	106864.0
31	129000.5
32	159436.5
33	190624.0
34	222452.5
35	247807.5
36	269898.5
37	309455.5
38	329648.5
39	330129.5
40	334188.0
41	341092.5
42	357151.5
43	401202.0
44	456235.5
45	500078.5
46	562438.5
47	631426.5
48	752035.5
49	890132.5
50	1112961.0
51	1088889.5
52	786000.0
53	673222.5
54	627535.0
55	577756.5
56	547029.5
57	496617.5
58	470825.0
59	446433.5
60	380240.5
61	313386.0
62	267860.0
63	210585.5
64	154875.5
65	128832.5
66	98367.5
67	67360.0
68	54150.5
69	48583.5
70	45988.0
71	43048.0
72	38585.5
73	42907.5
74	34862.5
75	24922.0
76	18750.5
77	12159.5
78	8642.0
79	5130.0
80	3260.0
81	2318.5
82	1774.0
83	1305.0
84	882.0
85	578.0
86	411.5
87	242.5
88	134.5
89	88.5
90	58.0
91	38.5
92	27.0
93	21.0
94	17.0
95	15.0
96	11.5
97	6.5
98	4.0
99	5.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08185632077932377
2	7.105998083410803E-4
3	0.0
4	0.0
5	0.06309409726833491
6	0.0
7	2.3885707843397654E-5
8	0.0
9	0.0
10-11	5.642998478002695E-4
12-13	2.985713480424707E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0010479854316290723
24-25	0.0013316282122694193
26-27	1.0748568529528945E-4
28-29	0.003475370491214359
30-31	0.0
32-33	1.4032853357996122E-4
34-35	1.73171381864633E-4
36-37	4.1799988725945894E-5
38-39	1.5525710098208476E-4
40-41	1.4928567402123534E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0013495424931519676
50-51	4.6577130294625426E-4
52-53	0.006338669718941653
54-55	8.95714044127412E-6
56-57	0.0021317994250232408
58-59	0.0018003852286960983
60-61	8.539140554014663E-4
62-63	0.0018212852230590712
64-65	0.00132565678530857
66-67	1.701856683842083E-4
68-69	0.0
70-71	1.283856796582624E-4
72-73	7.792712183908485E-4
74-75	0.004771170141718682
76-77	0.006329712578500379
78-79	0.004293455984850729
80-81	0.006682026769190494
82-83	8.150997801559451E-4
84-85	0.002209427975514283
86-87	2.5378564583610006E-4
88-89	0.003555984755185826
90-91	2.8662849412077184E-4
92-93	0.0033141419632714245
94-95	0.0016660281220769867
96-97	4.8368558382880255E-4
98-99	0.0
100	0.001039028291187798
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.6746416E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.21502816992908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.03639778686053	20.85936703880421
2	15.466648826504928	9.346504599744938
3	6.050491916738097	5.484473511185094
4	2.8718836082010752	3.4709617649021225
5	1.7438835548328993	2.6345745367176057
6	1.0169288761994049	1.8435920784707602
7	0.6899270505753544	1.4592315687831292
8	0.4939560401799742	1.1939916534995632
9	0.3469926193128407	0.9435952590565464
>10	1.9394926932034824	10.769921676305612
>50	0.16048587959211164	3.3760317684946757
>100	0.13456061579466339	8.488216545586166
>500	0.02323337861383247	4.945474475566333
>1k	0.02196769696364706	12.778488200060048
>5k	0.0020471466777455117	4.197446837659685
>10k+	0.0011023097495552754	8.208128485163478
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	187015	1.1167464130832532	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	166438	0.9938723605098547	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	73432	0.4384938245890942	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	52437	0.3131237155460607	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	50875	0.3037963466332139	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	48921	0.2921281783517142	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	48701	0.2908144644203273	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	47230	0.28203049536091784	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	35245	0.2104629432351376	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	33982	0.2029210309835848	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	32816	0.19595834714723437	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	29671	0.17717820935536296	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	25340	0.15131595918792415	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	19821	0.11835965379099624	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19247	0.11493205471546866	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	18544	0.11073414156199154	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	17958	0.10723488536293377	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	17520	0.10461940035408172	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	5.971426960849414E-6	0.0	5.971426960849414E-6	0.0
5	0.0	5.971426960849414E-6	0.0	2.3885707843397654E-5	0.0
6	2.3885707843397654E-5	5.971426960849414E-6	0.0	2.3885707843397654E-5	1.1942853921698827E-5
7	2.3885707843397654E-5	5.971426960849414E-6	0.0	2.3885707843397654E-5	1.7914280882548243E-5
8	2.3885707843397654E-5	5.971426960849414E-6	0.0	2.3885707843397654E-5	1.7914280882548243E-5
9	2.3885707843397654E-5	5.971426960849414E-6	0.0	2.985713480424707E-5	1.7914280882548243E-5
10-11	2.6871421323822362E-5	5.971426960849414E-6	0.0	6.269998308891886E-5	1.7914280882548243E-5
12-13	2.985713480424707E-5	5.971426960849414E-6	0.0	1.3734282009953653E-4	1.7914280882548243E-5
14-15	3.582856176509649E-5	5.971426960849414E-6	0.0	1.9705708970803066E-4	2.0899994362972947E-5
16-17	6.568569656934355E-5	5.971426960849414E-6	0.0	2.8065706715992245E-4	2.3885707843397654E-5
18-19	6.568569656934355E-5	5.971426960849414E-6	0.0	4.000856063769107E-4	2.3885707843397654E-5
20-21	6.568569656934355E-5	5.971426960849414E-6	0.0	5.762427017219684E-4	3.582856176509649E-5
22-23	7.165712353019297E-5	5.971426960849414E-6	0.0	0.0012002568191307322	5.075712916722002E-5
24-25	8.061426397146709E-5	5.971426960849414E-6	0.0	0.0024900850426742057	6.568569656934355E-5
26-27	1.2241425269741299E-4	5.971426960849414E-6	0.0	0.004215827434359686	6.568569656934355E-5
28-29	1.4331424706038595E-4	5.971426960849414E-6	0.0	0.01380295342000342	6.568569656934355E-5
30-31	1.4928567402123534E-4	5.971426960849414E-6	0.0	0.044499073712249836	6.568569656934355E-5
32-33	1.4928567402123534E-4	5.971426960849414E-6	0.0	0.1013649726604188	6.568569656934355E-5
34-35	1.5525710098208476E-4	5.971426960849414E-6	0.0	0.17512105276735035	6.568569656934355E-5
36-37	1.5525710098208476E-4	5.971426960849414E-6	0.0	0.28627617993008175	6.568569656934355E-5
38-39	1.5525710098208476E-4	1.1942853921698827E-5	0.0	0.47878602800742565	7.165712353019297E-5
40-41	1.582428144625095E-4	1.1942853921698827E-5	0.0	0.748398941003257	7.762855049104238E-5
42-43	1.6122852794293418E-4	1.1942853921698827E-5	0.0	0.9511378434645359	7.762855049104238E-5
44-45	1.6719995490378358E-4	1.4928567402123536E-5	0.0	1.1638699289447962	7.762855049104238E-5
46-47	1.7615709534505772E-4	2.0899994362972947E-5	0.0	1.4059366493702294	7.762855049104238E-5
48-49	1.8511423578633184E-4	2.3885707843397654E-5	0.0	1.6495469836650423	8.35999774518918E-5
50-51	1.9108566274718124E-4	2.985713480424707E-5	0.0	1.914549955047098	8.35999774518918E-5
52-53	1.9108566274718124E-4	4.17999887259459E-5	0.0	2.194860679443291	9.255711789316592E-5
54-55	1.9407137622760596E-4	4.17999887259459E-5	0.0	2.4517156387372676	1.0151425833444004E-4
56-57	1.9705708970803066E-4	4.17999887259459E-5	0.0	2.7379709186729864	1.0449997181486475E-4
58-59	1.9705708970803066E-4	4.17999887259459E-5	0.0	3.0643482163586526	1.1047139877571416E-4
60-61	2.149713705905789E-4	4.777141568679531E-5	0.0	3.384225615797434	1.1942853921698828E-4
62-63	2.2691422451227774E-4	4.777141568679531E-5	0.0	3.7069245144752165	1.2539996617783768E-4
64-65	2.2989993799270243E-4	4.777141568679531E-5	0.0	4.037822182370245	1.2539996617783768E-4
66-67	2.3587136495355185E-4	5.3742842647644723E-5	0.0	4.383319989184551	1.313713931386871E-4
68-69	2.3885707843397655E-4	5.3742842647644723E-5	0.0	4.797456363200341	1.3734282009953653E-4
70-71	2.3885707843397655E-4	5.3742842647644723E-5	0.0	5.319000793960928	1.4331424706038595E-4
72-73	2.567713593165248E-4	5.3742842647644723E-5	0.0	5.771894714666111	1.73171381864633E-4
74-75	2.687142132382236E-4	5.3742842647644723E-5	0.0	6.1998041849671	1.73171381864633E-4
76-77	2.7169992671864835E-4	5.6728556128069434E-5	0.0	6.652686162818361	1.73171381864633E-4
78-79	2.8364278064034714E-4	6.568569656934355E-5	0.0	7.114298366886383	1.73171381864633E-4
80-81	2.866284941207719E-4	6.568569656934355E-5	0.0	7.6348634836253915	1.8809994926675654E-4
82-83	2.896142076011966E-4	6.568569656934355E-5	0.0	8.222968425005089	2.0302851666888008E-4
84-85	2.95585634562046E-4	7.165712353019297E-5	0.0	8.769237549097072	2.0302851666888008E-4
86-87	3.0454277500332013E-4	8.061426397146709E-5	0.0	9.31643821579495	2.0302851666888008E-4
88	3.0454277500332013E-4	8.35999774518918E-5	0.0	9.755585911636256	2.0302851666888008E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	6265	0.0	21.605003	1
GTATAAG	6370	0.0	21.248877	1
CTAACGC	2935	0.0	20.81638	3
TAGGCAT	8055	0.0	20.77092	5
TAGTACT	7495	0.0	20.316301	4
CTAGGCA	9180	0.0	19.710121	4
GTACATA	4230	0.0	19.66599	1
CATGGGG	40105	0.0	19.230091	4
ATGGGTA	10900	0.0	19.230015	5
TGATCGC	4515	0.0	19.152817	9
CTATTGA	8630	0.0	18.679096	9
TCTAACG	3355	0.0	18.630863	2
GGGCTAT	9345	0.0	18.607742	6
GGGTACC	9830	0.0	18.550291	7
TACGGGC	4230	0.0	18.443316	4
GTGATCG	4720	0.0	18.420536	8
TTTAGGG	9460	0.0	18.232609	2
CATGGGT	14540	0.0	18.003712	4
ATACGGG	4365	0.0	17.980518	3
AGGGCTA	9365	0.0	17.664696	5
>>END_MODULE