FastQCFastQC Report
Fri 27 May 2016
ERR765505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6456679
Sequences flagged as poor quality0
Sequence length99
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA123930.19194077946263086No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT114960.17804818855018192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG19650.019.641181
GTACATG394150.018.1209811
CATGGGG130900.018.0811824
GTACATA15700.018.0668411
GTACTAG17900.017.9245431
ATGGGTA38150.017.7953685
TACATGG385950.017.7349012
ACATGGG366400.017.0186613
CATGGGT50350.016.900534
GTATCAA264800.016.5067621
TAGTACT18550.016.293644
GTATTAG32050.016.104491
TCTAACG6950.016.057522
GTACTAA6500.015.738431
GTATTAT20350.015.5380511
GTCCTAT39200.015.3022641
GTATAAT15300.015.1960471
CTAACGC8600.015.13950353
GTATTAC11750.015.0382671
TAGGCAT24900.014.9396125
Produced by FastQC (version 0.11.5)