FastQCFastQC Report
Fri 27 May 2016
ERR765505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6456679
Sequences flagged as poor quality0
Sequence length99
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT125220.1939387105971971No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123740.19164651053583429No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA120750.18701564689835132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76730.11883818291106002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67680.10482168929259145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA333900.026.2665461
AGTACTT198700.021.97744812-13
TATCACG5300.021.9349522
CAACGCA407250.021.3184575
ATCAACG407850.021.2414863
AACGCAG420950.020.8662156
TCAACGC418850.020.7502484
TATCAAC439900.019.9898762
ACGCAGA455800.019.107587
CGCAGAG458650.019.0091258
TAACGCA4250.018.6008384
TACTTTT237050.018.55926114-15
GAGTACT194550.018.22712312-13
GTATAAG19500.018.1337241
GCAGAGT482200.018.0618789
CTAGTAC21750.017.5318263
GTATAAT16900.017.3445031
GGTATCA243750.017.1029641
AGAGTAC460200.016.85604110-11
TAGTACT19750.016.4817544
Produced by FastQC (version 0.11.5)