FastQCFastQC Report
Fri 27 May 2016
ERR765503_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765503_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6999096
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA224760.32112718556796477No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT197880.2827222258417373No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG87750.1253733339276958No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG76930.10991419463313547No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT72630.10377054408169284No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG22300.024.6052471
CTAGGCA35400.018.2582454
GTATAAG28550.017.2643551
TAGGCAT35450.017.0519735
CATGGGG162250.016.9088784
TAGTACT28400.016.7004974
TACTAGG34250.016.699062
ACTAGGC34300.016.4035843
GTACAAG39300.016.3281571
GTACATG580150.015.9100241
TACATGG565000.015.75219252
GTATAAT24400.015.6269651
ATACGGG13250.015.0903773
TATACCG10300.014.8978725
ATATACG23450.014.87188156
GTACATA19700.014.6344391
ACATGGG531500.014.6278513
GTGATCG18500.014.5781968
TACGGGC14500.014.11016754
CGGGCGT14200.014.0808116
Produced by FastQC (version 0.11.5)