##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765503_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6999096 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.261281885546364 33.0 33.0 33.0 27.0 33.0 2 31.155935995162803 33.0 33.0 33.0 27.0 33.0 3 31.21643423664999 33.0 33.0 33.0 27.0 33.0 4 34.760635516358114 37.0 37.0 37.0 27.0 37.0 5 34.922193809029054 37.0 37.0 37.0 33.0 37.0 6 34.9338616015554 37.0 37.0 37.0 33.0 37.0 7 34.810071614962844 37.0 37.0 37.0 33.0 37.0 8 34.73114299332371 37.0 37.0 37.0 27.0 37.0 9 34.684831155337775 37.0 37.0 37.0 27.0 37.0 10-11 34.66636048426825 37.0 37.0 37.0 27.0 37.0 12-13 34.602382436246046 37.0 37.0 37.0 27.0 37.0 14-15 36.01520696387076 37.0 37.0 40.0 27.0 40.0 16-17 36.06544723775756 37.0 37.0 40.0 27.0 40.0 18-19 36.07808729870258 37.0 37.0 40.0 27.0 40.0 20-21 36.09052726237789 37.0 37.0 40.0 27.0 40.0 22-23 36.144819630992345 37.0 37.0 40.0 27.0 40.0 24-25 36.109199102284066 37.0 37.0 40.0 27.0 40.0 26-27 36.120446626250015 37.0 37.0 40.0 27.0 40.0 28-29 36.05344733091245 37.0 37.0 40.0 27.0 40.0 30-31 36.01958645802258 37.0 37.0 40.0 27.0 40.0 32-33 35.89141783453178 37.0 37.0 40.0 27.0 40.0 34-35 35.853408640201536 37.0 37.0 40.0 27.0 40.0 36-37 35.750725307954056 37.0 37.0 40.0 27.0 40.0 38-39 35.548492190991524 37.0 33.0 40.0 27.0 40.0 40-41 35.47705653701564 37.0 33.0 40.0 27.0 40.0 42-43 35.35153432671876 37.0 33.0 40.0 27.0 40.0 44-45 35.217897925674976 37.0 33.0 40.0 27.0 40.0 46-47 35.098941134683685 37.0 33.0 40.0 27.0 40.0 48-49 34.937726100627856 37.0 33.0 40.0 27.0 40.0 50-51 34.77943108652889 37.0 33.0 40.0 27.0 40.0 52-53 34.59971544896655 37.0 33.0 40.0 27.0 40.0 54-55 34.47659562034868 37.0 33.0 40.0 27.0 40.0 56-57 34.284936440363154 37.0 33.0 40.0 27.0 40.0 58-59 34.055700764784476 37.0 33.0 38.5 27.0 40.0 60-61 33.818321751837665 37.0 33.0 37.0 24.5 40.0 62-63 33.6399513594327 37.0 33.0 37.0 22.0 40.0 64-65 33.341206207201616 37.0 33.0 37.0 22.0 40.0 66-67 33.12391650293124 37.0 33.0 37.0 22.0 40.0 68-69 32.82940932657589 37.0 33.0 37.0 22.0 40.0 70-71 32.67772352315213 37.0 33.0 37.0 22.0 38.5 72-73 32.39396973552013 35.0 33.0 37.0 22.0 37.0 74-75 32.1076979084156 33.0 33.0 37.0 22.0 37.0 76-77 31.911985990762236 33.0 33.0 37.0 22.0 37.0 78-79 31.66883630400269 33.0 33.0 37.0 22.0 37.0 80-81 31.3988447936705 33.0 27.0 37.0 22.0 37.0 82-83 31.20689364740818 33.0 27.0 37.0 22.0 37.0 84-85 31.011342036171527 33.0 27.0 37.0 22.0 37.0 86-87 30.791221537752875 33.0 27.0 37.0 15.0 37.0 88-89 30.605407826953652 33.0 27.0 37.0 15.0 37.0 90-91 30.392152500837252 33.0 27.0 37.0 15.0 37.0 92-93 30.232551818120513 33.0 27.0 37.0 15.0 37.0 94-95 29.995891683726015 33.0 27.0 37.0 15.0 37.0 96-97 29.908121634565376 33.0 27.0 37.0 15.0 37.0 98-99 28.778059052197598 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34924044265119747 1101 2 0.26797491268998996 1101 3 0.29952745160972327 1101 4 0.4328531523576018 1101 5 0.38499555813127273 1101 6 0.3195668104212359 1101 7 0.2957884363984675 1101 8 0.28277254264660456 1101 9 0.2702483883726785 1101 10-11 0.3153134647146061 1101 12-13 0.2643921210142608 1101 14-15 0.3702765406827808 1101 16-17 0.3829061586351443 1101 18-19 0.35997686613046653 1101 20-21 0.35544893736867067 1101 22-23 0.3357254792512734 1101 24-25 0.46119509013630733 1101 26-27 0.44568159842674504 1101 28-29 0.3540618305088543 1101 30-31 0.40653997177455636 1101 32-33 0.4849670113652351 1101 34-35 0.41088043790868767 1101 36-37 0.38436606401290163 1101 38-39 0.3367928416830708 1101 40-41 0.36231076331895906 1101 42-43 0.4155133292010049 1101 44-45 0.33947951398918264 1101 46-47 0.39577893196140934 1101 48-49 0.3614984207722003 1101 50-51 0.34096084491010714 1101 52-53 0.252672561778553 1101 54-55 0.4004819252577789 1101 56-57 0.4639862788927047 1101 58-59 0.4095994740866473 1101 60-61 0.432377224596884 1101 62-63 0.4026285591863612 1101 64-65 0.33666925392827096 1101 66-67 0.3736791223548934 1101 68-69 0.4125608181721674 1101 70-71 0.4163039204820578 1101 72-73 0.4981925671339198 1101 74-75 0.4324087525416189 1101 76-77 0.31239573232130624 1101 78-79 0.34829333804466955 1101 80-81 0.2758188467784528 1101 82-83 0.31604634610683746 1101 84-85 0.19611801585593014 1101 86-87 0.24111466562759887 1101 88-89 0.3074391282480349 1101 90-91 0.33373667334968715 1101 92-93 0.39122268274505956 1101 94-95 0.39687558353097785 1101 96-97 0.4036291350421557 1101 98-99 0.39911345364159345 1102 1 0.2432826642556556 1102 2 0.2843781808385266 1102 3 0.3262775995058149 1102 4 0.4256863782202913 1102 5 0.2671958004122601 1102 6 0.30117162669733233 1102 7 0.2917345564445242 1102 8 0.21423446177161054 1102 9 0.29118228448556494 1102 10-11 0.2699153529558842 1102 12-13 0.3157690146319396 1102 14-15 0.3802974230848051 1102 16-17 0.41204155555158906 1102 18-19 0.36106534388218847 1102 20-21 0.4105540016314606 1102 22-23 0.27369291765794657 1102 24-25 0.4246149034905997 1102 26-27 0.45579006977666126 1102 28-29 0.3933100795123252 1102 30-31 0.39939989476918925 1102 32-33 0.5376754255136476 1102 34-35 0.4659292791293481 1102 36-37 0.47716232831977123 1102 38-39 0.469954081835958 1102 40-41 0.38039437302708734 1102 42-43 0.4710035028164512 1102 44-45 0.4167480857952839 1102 46-47 0.4781778585114367 1102 48-49 0.47871521372733383 1102 50-51 0.46714152449579416 1102 52-53 0.4820970520920511 1102 54-55 0.5182047470873599 1102 56-57 0.40859351326690785 1102 58-59 0.5064465273268581 1102 60-61 0.5250675601869759 1102 62-63 0.5097315542623662 1102 64-65 0.3846555258073252 1102 66-67 0.3147176552325348 1102 68-69 0.37126661877022826 1102 70-71 0.3355870594126955 1102 72-73 0.33186097455103436 1102 74-75 0.3871099618520475 1102 76-77 0.3461700804925627 1102 78-79 0.3906954383290966 1102 80-81 0.3848022270442826 1102 82-83 0.353405448446253 1102 84-85 0.2909595556096214 1102 86-87 0.4183408375732256 1102 88-89 0.4430020398116241 1102 90-91 0.44571079874734565 1102 92-93 0.49519805507503634 1102 94-95 0.45739596970167895 1102 96-97 0.36999542185921896 1102 98-99 0.4161136713801348 1103 1 0.18386273289980437 1103 2 0.24626297262464192 1103 3 0.22310256379380533 1103 4 0.263171801203832 1103 5 0.2532515465327094 1103 6 0.18850510135265353 1103 7 0.1414240236364961 1103 8 0.14408499490524207 1103 9 0.22332590113821738 1103 10-11 0.1568233066954008 1103 12-13 0.1767798668895182 1103 14-15 0.15263136150601753 1103 16-17 0.13568144442043462 1103 18-19 0.16412446871296993 1103 20-21 0.16957080249793677 1103 22-23 0.12171096683466232 1103 24-25 0.17483910090116694 1103 26-27 0.2618406332411638 1103 28-29 0.2049423899470426 1103 30-31 0.21212804965428234 1103 32-33 0.29371866117131873 1103 34-35 0.21051650336325878 1103 36-37 0.302130380475532 1103 38-39 0.2613895113332916 1103 40-41 0.2661876487310906 1103 42-43 0.2222688622785114 1103 44-45 0.17590470043418094 1103 46-47 0.11964671772859248 1103 48-49 0.17725975613758038 1103 50-51 0.20995756327035764 1103 52-53 0.17439844584022524 1103 54-55 0.16629206290186715 1103 56-57 0.10341332654174806 1103 58-59 0.2169732786990295 1103 60-61 0.262313259204511 1103 62-63 0.1297620623760949 1103 64-65 0.10906583703756922 1103 66-67 0.15642323727775675 1103 68-69 0.17620741546121366 1103 70-71 0.07939814280887703 1103 72-73 0.1937434653514245 1103 74-75 0.2000768482543549 1103 76-77 0.13780557169166485 1103 78-79 0.09984272411931272 1103 80-81 0.0674878881271681 1103 82-83 0.06946177100271456 1103 84-85 0.16195997423947261 1103 86-87 0.2748602642654667 1103 88-89 0.17348061459262354 1103 90-91 0.18098284170623913 1103 92-93 0.24416482496657466 1103 94-95 0.18366238109148725 1103 96-97 0.05520893413106975 1103 98-99 0.1724293291325374 1104 1 0.16984335953631557 1104 2 0.11999397188879968 1104 3 0.07556319540687184 1104 4 0.14220420118779487 1104 5 0.15501832541738736 1104 6 0.16967533223036924 1104 7 0.10795925771869008 1104 8 0.09119990336694173 1104 9 0.10458649575453904 1104 10-11 0.1208897035804739 1104 12-13 0.10404923268177413 1104 14-15 0.1748473152895471 1104 16-17 0.11558912034529811 1104 18-19 0.14311970824645215 1104 20-21 0.19690220127786517 1104 22-23 0.24155884226366453 1104 24-25 0.18432115461357057 1104 26-27 0.200927267838523 1104 28-29 0.1118305991980364 1104 30-31 0.11212008962386477 1104 32-33 0.18432282747988893 1104 34-35 0.18409375535613037 1104 36-37 0.27081144640750665 1104 38-39 0.16680731470867727 1104 40-41 0.23760505185404668 1104 42-43 0.15317728182164103 1104 44-45 0.1797517585788242 1104 46-47 0.07466425844343405 1104 48-49 0.1385513969187997 1104 50-51 0.22117440227474816 1104 52-53 0.10474204086006722 1104 54-55 0.2632727558378605 1104 56-57 0.16516720158276854 1104 58-59 0.2495933912608166 1104 60-61 0.2467799453517685 1104 62-63 0.1681871652426068 1104 64-65 0.15839786975512737 1104 66-67 0.10674169988279658 1104 68-69 0.09046381970471629 1104 70-71 0.14014795925753987 1104 72-73 0.07898228468042134 1104 74-75 0.011026739760566784 1104 76-77 -0.12728415974632412 1104 78-79 0.07753632474596373 1104 80-81 0.11139277619972532 1104 82-83 0.19017742275527638 1104 84-85 0.12563476422972641 1104 86-87 0.15248436298130486 1104 88-89 0.17240519197434878 1104 90-91 0.18215310941700125 1104 92-93 0.1854546895764848 1104 94-95 0.11998694652293551 1104 96-97 -0.11666633444322372 1104 98-99 0.06667142373780521 1105 1 -0.05388517064227827 1105 2 0.04361117757240507 1105 3 -0.02731647135439985 1105 4 -0.11254026162329467 1105 5 -0.0787756212782611 1105 6 -0.06563038652113562 1105 7 -0.08081100909203798 1105 8 -0.14301059855501563 1105 9 -0.14522841781545281 1105 10-11 -0.09483628371300767 1105 12-13 -0.06989118414200135 1105 14-15 -0.03456446769904886 1105 16-17 -0.080547490956981 1105 18-19 -0.09248352560982198 1105 20-21 -0.05376860497239022 1105 22-23 -0.050542407749624374 1105 24-25 -0.0014229780341352694 1105 26-27 0.044789739112808036 1105 28-29 0.03540322873535473 1105 30-31 0.016074784446296064 1105 32-33 0.09044408318583663 1105 34-35 -0.028377951741234142 1105 36-37 0.014129284418061161 1105 38-39 -0.05136318686975727 1105 40-41 -0.13245011419414965 1105 42-43 -0.0733267439360148 1105 44-45 -0.16771280951920886 1105 46-47 -0.1692555620196643 1105 48-49 -0.18180349600166323 1105 50-51 -0.03250290827595137 1105 52-53 -0.08085435662393081 1105 54-55 0.022181441806679913 1105 56-57 0.0840738877593381 1105 58-59 -0.09808816179392466 1105 60-61 0.030020016390061244 1105 62-63 -0.04717084732650534 1105 64-65 -0.17248026533158622 1105 66-67 -0.1274336318686906 1105 68-69 -0.2250114676614814 1105 70-71 -0.19934289731605048 1105 72-73 -0.19188161807432635 1105 74-75 -0.12419476142771657 1105 76-77 -0.21579335279266587 1105 78-79 -0.06565336706791669 1105 80-81 -0.1738521308733212 1105 82-83 -0.10582033794089085 1105 84-85 -0.19245957620825394 1105 86-87 -0.1450352573389857 1105 88-89 -0.17339586454847478 1105 90-91 -0.14394038298365075 1105 92-93 -0.13301713380055702 1105 94-95 -0.15460378447406242 1105 96-97 -0.2109675467780825 1105 98-99 -0.08703125227594555 1106 1 0.007373587154152261 1106 2 0.13125082117159437 1106 3 0.034001148787240254 1106 4 0.034321264529339146 1106 5 -0.014491971073077536 1106 6 -0.02067591148556147 1106 7 0.060288437570058306 1106 8 -0.03306810373793212 1106 9 -0.013281189731401355 1106 10-11 -0.041521699865683104 1106 12-13 -0.040987379080810626 1106 14-15 -0.034797607355343985 1106 16-17 0.03450811782051488 1106 18-19 0.06960939738407035 1106 20-21 -0.013809246734766134 1106 22-23 0.005987383941224778 1106 24-25 0.05725600606801606 1106 26-27 0.09915082520213048 1106 28-29 -0.04852848534384435 1106 30-31 -0.09816456096618964 1106 32-33 0.042086742522378984 1106 34-35 -0.09041575322149242 1106 36-37 -0.08221045441723618 1106 38-39 -0.07770806025207833 1106 40-41 0.01932105637224879 1106 42-43 -0.02960347784497941 1106 44-45 -0.027149684252464112 1106 46-47 -0.046313962554101806 1106 48-49 -0.17149190049357088 1106 50-51 -0.1114733968875683 1106 52-53 -0.10799935045063336 1106 54-55 -0.1277135449525204 1106 56-57 -0.06212779074893149 1106 58-59 -0.012777395439229622 1106 60-61 0.028330677142982097 1106 62-63 -0.044380343753566365 1106 64-65 -0.12316420872674883 1106 66-67 -0.03707535024609143 1106 68-69 -0.017577364731842238 1106 70-71 1.6820483822499455E-4 1106 72-73 0.05639523937765745 1106 74-75 -0.04032639081087552 1106 76-77 -0.05622654326792542 1106 78-79 -0.04214875484560565 1106 80-81 -0.06937470005967228 1106 82-83 -0.07675287778644702 1106 84-85 -0.043017069100592664 1106 86-87 -0.10877633773608153 1106 88-89 0.022102122629274135 1106 90-91 -0.009659247401998527 1106 92-93 0.021259211806405887 1106 94-95 -0.07733513109663193 1106 96-97 -0.1617519988223819 1106 98-99 0.23825103798354164 1107 1 0.02974033809537957 1107 2 0.10140794611638526 1107 3 -0.004473228822710951 1107 4 0.010349402661830709 1107 5 0.014006005500050378 1107 6 5.085965739439757E-4 1107 7 -0.13584687067674395 1107 8 -0.06165865764776868 1107 9 -0.24431864356731836 1107 10-11 -0.0894115245856213 1107 12-13 -0.1420728261533668 1107 14-15 -0.14373099004001944 1107 16-17 -0.14467620953268323 1107 18-19 -0.22166734517307418 1107 20-21 -0.08103614917268942 1107 22-23 -0.15112220321755387 1107 24-25 -0.03664005350939448 1107 26-27 0.04590995157847999 1107 28-29 -0.08580041572190567 1107 30-31 -0.13236376613227918 1107 32-33 -0.23439228251137223 1107 34-35 -0.2234114048751934 1107 36-37 -0.14711692313144908 1107 38-39 -0.11139273294654828 1107 40-41 -0.21242658306957907 1107 42-43 -0.2662644218684065 1107 44-45 -0.18043730050953144 1107 46-47 -0.264851965484425 1107 48-49 -0.2006044187894247 1107 50-51 -0.20058564396627077 1107 52-53 -0.2425265906078593 1107 54-55 -0.2344758334818806 1107 56-57 -0.16853835594182698 1107 58-59 -0.13215600390649485 1107 60-61 -0.08713600562629864 1107 62-63 -0.2563625973213064 1107 64-65 -0.2701991076771506 1107 66-67 -0.09634888430760924 1107 68-69 -0.23110255592976614 1107 70-71 -0.1278140001959187 1107 72-73 -0.12574531761822527 1107 74-75 -0.19151519875778433 1107 76-77 -0.3043867709934034 1107 78-79 -0.18412446210429678 1107 80-81 -0.33046474919271773 1107 82-83 -0.25070721635341187 1107 84-85 -0.3092659581621753 1107 86-87 -0.3795037789110225 1107 88-89 -0.23094607228569686 1107 90-91 -0.17522782624062216 1107 92-93 -0.20994020879806286 1107 94-95 -0.17780737676380198 1107 96-97 -0.2924645796542009 1107 98-99 0.08438727615330066 1108 1 -0.08272721894268642 1108 2 -0.031451988087074056 1108 3 -0.060754536607344534 1108 4 -0.09284639619161084 1108 5 -0.07105983892860479 1108 6 -0.16870213078335894 1108 7 -0.12090543165689382 1108 8 -0.18950187229878424 1108 9 -0.09184672289472928 1108 10-11 -0.18885430983903007 1108 12-13 -0.14161516671879326 1108 14-15 -0.12034582416510631 1108 16-17 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0.22885927167681785 2215 92-93 0.17662676506951414 2215 94-95 0.25482784022815963 2215 96-97 0.24279570328654643 2215 98-99 0.14391418490709285 2216 1 -0.04366158521233032 2216 2 -0.03540320320646373 2216 3 -0.014573680691860602 2216 4 -0.02519219942185913 2216 5 -0.05849244612234372 2216 6 -0.02550941716603461 2216 7 -0.03457097759579142 2216 8 -0.1513021627558686 2216 9 -0.013735333009634587 2216 10-11 -0.1347149530381344 2216 12-13 -0.05283286669003928 2216 14-15 -0.08724915077120698 2216 16-17 -0.02928195664820521 2216 18-19 -0.0949689457113152 2216 20-21 -0.110229843551366 2216 22-23 -0.19659220611933392 2216 24-25 -0.12783117222470963 2216 26-27 -0.22033463553150057 2216 28-29 -0.003983894495853235 2216 30-31 -0.09508604619730221 2216 32-33 -0.04146563765163336 2216 34-35 -0.19183301487076676 2216 36-37 -0.16754435159350578 2216 38-39 -0.0894087490092943 2216 40-41 -0.129967198445037 2216 42-43 -0.14509221376165726 2216 44-45 -0.13837310076625897 2216 46-47 -4.649052764094108E-4 2216 48-49 0.09562361206608472 2216 50-51 0.01212454079954739 2216 52-53 0.09877153817802053 2216 54-55 -0.014016876944396017 2216 56-57 0.10694781544763288 2216 58-59 -0.09345184164079257 2216 60-61 0.02172940510316579 2216 62-63 0.03126084625769465 2216 64-65 0.13944718695073988 2216 66-67 -0.04013942899397449 2216 68-69 -0.055381214971383486 2216 70-71 -0.06222879360360878 2216 72-73 -0.11681353749126089 2216 74-75 -0.03342131219989142 2216 76-77 0.015945027815597257 2216 78-79 0.011099252752430289 2216 80-81 0.14527967379072138 2216 82-83 0.07553385138701785 2216 84-85 0.06267822081974828 2216 86-87 -0.010700772129567326 2216 88-89 0.037026626312528066 2216 90-91 0.193717092741295 2216 92-93 0.28243465420308667 2216 94-95 0.17354284608205006 2216 96-97 0.2461150279254909 2216 98-99 0.1877025193159021 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 208.0 8 1160.0 9 2157.0 10 3538.0 11 6583.0 12 12859.0 13 23423.0 14 32919.0 15 37209.0 16 39034.0 17 40437.0 18 41706.0 19 44961.0 20 49119.0 21 53560.0 22 60193.0 23 66973.0 24 76575.0 25 86886.0 26 97439.0 27 110541.0 28 130141.0 29 159415.0 30 199084.0 31 249347.0 32 323074.0 33 425883.0 34 572192.0 35 812833.0 36 1225448.0 37 1511671.0 38 501974.0 39 550.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.791668750312546 18.726794733495737 12.536037548489418 24.9454989677023 2 15.680096401020933 20.941175831850284 38.15588470282448 25.222843064304307 3 18.150284194250506 26.44190970078093 28.952680392839863 26.455125712128698 4 12.93729933122792 17.573140874192898 36.449064279158335 33.04049551542085 5 14.278615409761489 37.720357028964884 33.37552449630638 14.625503064967246 6 31.61481139850061 36.717970435039035 17.766851604835825 13.90036656162453 7 27.6737595826661 32.116147571057745 22.099353973713175 18.11073887256297 8 26.717407505197816 33.973901772457474 20.104696377932235 19.20399434441248 9 27.0133028608266 15.871235370967906 19.2565154128476 37.858946355357894 10-11 25.100341529820426 26.209306173254376 27.072717676682817 21.617634620242384 12-13 25.955166210036268 24.088317977064467 26.550764555879784 23.405751257019478 14-15 24.029488951144547 24.881584707510797 23.937977133046896 27.15094920829776 16-17 23.832449219156302 27.335058699009128 24.96311666535221 23.86937541648236 18-19 24.25954723295694 26.932785319704145 25.91524676901131 22.892420678327603 20-21 25.423030900511854 26.288688148076712 24.97084971942721 23.317431231984227 22-23 24.808475265948633 26.168115139440868 24.66354226317227 24.35986733143823 24-25 24.222323604710407 26.642803521404673 24.766859966525683 24.368012907359233 26-27 24.41729601002644 26.459674637888845 24.653912349226914 24.4691170028578 28-29 24.19967778924845 26.392489846074707 25.23620334284724 24.1716290218296 30-31 24.66448537421767 26.350797174735497 24.98577845043939 23.99893900060744 32-33 24.02397511162699 26.699472352947524 24.7853239238264 24.49122861159909 34-35 24.197945962133375 26.72347355914167 25.1558344996751 23.922745979049854 36-37 24.432512427319185 26.561487369225965 24.910867060546103 24.095133142908743 38-39 24.4974243098572 26.34947089588011 24.894818544397527 24.25828624986516 40-41 24.22051362061615 26.553150578303253 25.147576201269423 24.078759599811175 42-43 24.665613959288457 26.417047287249666 24.91418891811171 24.003149835350165 44-45 24.323412623573102 26.6257242363871 24.8965509260053 24.154312214034498 46-47 23.944399391007067 26.573867539465095 25.43824945392948 24.04348361559836 48-49 24.357096550085995 26.536773543694085 25.251627444291845 23.854502461928075 50-51 23.68557310829856 26.813477054751072 25.130023934519546 24.37092590243083 52-53 23.761682937339337 26.711363867562326 24.74348830191785 24.78346489318049 54-55 24.013294002539755 26.41800455373094 24.83807908907093 24.730622354658376 56-57 24.209805094829388 26.054936237479815 24.81298299094626 24.92227567674454 58-59 24.011672364545365 26.01913161356838 25.3935651118373 24.575630910048954 60-61 24.07404470520193 26.25238316491158 25.092561953715165 24.581010176171322 62-63 23.58576736195646 26.234309402242808 25.119115382900876 25.060807852899856 64-65 23.531617511747232 26.05179297440698 25.267091635834117 25.149497878011672 66-67 24.139652932222372 25.89712325623859 25.027039213932547 24.936184597606488 68-69 24.114057015363127 25.554064410603882 25.133345792085148 25.19853278194784 70-71 23.597633180056395 25.6770445783284 25.271192165388218 25.45413007622699 72-73 24.18041558509842 25.62198746809588 25.274171121527694 24.923425825278006 74-75 24.26896273461601 25.517309663990893 25.29550959152439 24.918218009868703 76-77 23.860517129640744 25.673994184391812 25.578589006351677 24.886899679615766 78-79 23.97498905572948 25.617279717266346 25.519652823736095 24.88807840326808 80-81 23.631408970529908 25.561301059451107 25.786673021773098 25.020616948245888 82-83 23.84102175480948 25.662285529445516 25.407038280372205 25.0896544353728 84-85 23.95076449872955 25.73680229561075 25.09274769198765 25.21968551367205 86-87 23.745852321499804 25.389314562909266 25.436156326474162 25.428676789116768 88-89 23.96461628758914 25.021295607318432 25.650362561107894 25.36372554398454 90-91 24.153404954011204 25.24798202510724 25.45118683898606 25.147426181895487 92-93 23.899965081204773 25.341394088608016 25.442692884909707 25.315947945277507 94-95 23.440077118530738 25.421811616814516 25.79347390005795 25.3446373645968 96-97 24.006064497472245 25.174565401017503 25.453587148968953 25.3657829525413 98-99 23.997284792207452 25.230186869847195 25.226722136687368 25.545806201257992 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1160.0 1 1086.5 2 1028.5 3 1014.5 4 926.0 5 830.0 6 770.5 7 700.0 8 665.0 9 641.0 10 596.5 11 613.5 12 643.5 13 648.0 14 705.5 15 888.5 16 1069.0 17 1298.5 18 1764.5 19 2422.5 20 3427.5 21 4778.0 22 6610.0 23 9082.0 24 12011.5 25 16336.0 26 21968.5 27 27943.0 28 35575.5 29 43843.0 30 51486.5 31 61675.5 32 72670.0 33 84177.5 34 95656.5 35 107754.0 36 118985.0 37 130676.5 38 144134.5 39 149182.5 40 155584.5 41 164655.0 42 174159.5 43 191372.0 44 209609.0 45 228394.5 46 253751.5 47 279618.5 48 300706.5 49 321156.5 50 343790.0 51 332078.5 52 303322.5 53 297126.0 54 285931.0 55 272040.0 56 251285.0 57 231236.0 58 217198.0 59 192353.5 60 162780.0 61 129861.0 62 104258.5 63 83554.0 64 62400.5 65 47302.5 66 35745.0 67 27609.5 68 22042.0 69 18575.0 70 15967.5 71 13572.0 72 12468.5 73 10834.0 74 8164.5 75 6238.0 76 4643.0 77 3278.0 78 2308.5 79 1588.5 80 1093.5 81 746.5 82 514.5 83 363.0 84 270.5 85 193.5 86 125.5 87 98.5 88 72.5 89 52.5 90 36.0 91 28.5 92 20.5 93 12.0 94 7.5 95 6.0 96 8.0 97 6.5 98 3.5 99 7.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0020859836756061067 2 0.0 3 1.4287559421959637E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.000387478611524E-4 22-23 0.0 24-25 0.0042576927077439715 26-27 2.857511884391927E-5 28-29 0.008158196429938952 30-31 4.929208000576074E-4 32-33 6.429401739881837E-5 34-35 5.715023768783854E-5 36-37 0.0 38-39 1.4287559421959635E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.2144425508665691E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.857511884391927E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6999096.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.92109849156792 #Duplication Level Percentage of deduplicated Percentage of total 1 76.4564516457875 35.10964246342007 2 13.035233159021642 11.971844515119697 3 4.270019425506208 5.882519477987364 4 1.9058639593913171 3.5007746636295307 5 1.0365024786853714 2.379866620523261 6 0.641634046797565 1.7678724155120569 7 0.46042229977019233 1.480016844282285 8 0.31984851110396634 1.1750235984629276 9 0.22842365836008538 0.9440518782021966 >10 1.3825433080676048 11.885598605403928 >50 0.13563518689855275 4.3740463209676985 >100 0.10943897881554236 10.21551391315612 >500 0.011801657672125172 3.607131793520727 >1k 0.005869477853946139 4.335765970384573 >5k 2.497650148484241E-4 0.7643980878116255 >10k+ 6.244125371210602E-5 0.6059328316160051 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 22476 0.32112718556796477 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 19788 0.2827222258417373 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 8775 0.1253733339276958 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 7693 0.10991419463313547 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 7263 0.10377054408169284 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.857511884391927E-5 2 0.0 1.4287559421959635E-5 0.0 0.0 2.857511884391927E-5 3 0.0 1.4287559421959635E-5 0.0 0.0 2.857511884391927E-5 4 0.0 4.286267826587891E-5 0.0 0.0 2.857511884391927E-5 5 0.0 4.286267826587891E-5 0.0 1.4287559421959635E-5 2.857511884391927E-5 6 0.0 4.286267826587891E-5 0.0 1.4287559421959635E-5 4.286267826587891E-5 7 0.0 4.286267826587891E-5 0.0 1.4287559421959635E-5 4.286267826587891E-5 8 0.0 4.286267826587891E-5 0.0 2.857511884391927E-5 5.715023768783854E-5 9 0.0 5.715023768783854E-5 0.0 2.857511884391927E-5 7.143779710979818E-5 10-11 1.4287559421959635E-5 5.715023768783854E-5 0.0 2.857511884391927E-5 1.0001291595371745E-4 12-13 2.857511884391927E-5 5.715023768783854E-5 0.0 4.286267826587891E-5 1.0001291595371745E-4 14-15 2.857511884391927E-5 7.143779710979818E-5 0.0 5.000645797685873E-5 1.4287559421959637E-4 16-17 3.571889855489909E-5 7.143779710979818E-5 0.0 7.143779710979818E-5 1.57163153641556E-4 18-19 4.286267826587891E-5 7.143779710979818E-5 0.0 8.572535653175782E-5 1.57163153641556E-4 20-21 4.286267826587891E-5 7.143779710979818E-5 0.0 2.5717606959527347E-4 1.57163153641556E-4 22-23 4.286267826587891E-5 7.8581576820778E-5 0.0 0.001293024127687347 1.57163153641556E-4 24-25 5.715023768783854E-5 1.0001291595371746E-4 0.0 0.003443301820692272 1.7145071306351564E-4 26-27 7.143779710979818E-5 1.1430047537567708E-4 0.0 0.0061936570094195025 1.7859449277449546E-4 28-29 1.1430047537567708E-4 1.1430047537567708E-4 0.0 0.01690218279617825 1.8573827248547528E-4 30-31 1.2144425508665691E-4 1.2858803479763673E-4 0.0 0.04704893317651308 1.8573827248547528E-4 32-33 1.4287559421959637E-4 1.2858803479763673E-4 0.0 0.09839842173903601 2.000258319074349E-4 34-35 1.57163153641556E-4 1.4287559421959637E-4 0.0 0.16364256183941467 2.000258319074349E-4 36-37 1.57163153641556E-4 1.57163153641556E-4 0.0 0.26315541321336355 2.000258319074349E-4 38-39 1.57163153641556E-4 1.7145071306351564E-4 0.0 0.4115531491495473 2.000258319074349E-4 40-41 1.57163153641556E-4 2.000258319074349E-4 0.0 0.5917192734604584 2.1431339132939453E-4 42-43 1.57163153641556E-4 2.1431339132939453E-4 0.0 0.7521257030907991 2.2860095075135416E-4 44-45 1.57163153641556E-4 2.2860095075135416E-4 0.0 0.9339991907526344 2.2860095075135416E-4 46-47 1.57163153641556E-4 2.500322898842936E-4 0.0 1.1350680144978722 2.3574473046233398E-4 48-49 1.7145071306351564E-4 2.5717606959527347E-4 0.0 1.3448593932702164 2.428885101733138E-4 50-51 1.7145071306351564E-4 2.8575118843919274E-4 0.0 1.5724459273026117 2.428885101733138E-4 52-53 1.7145071306351564E-4 3.000387478611524E-4 0.0 1.802047007213503 2.428885101733138E-4 54-55 1.7145071306351564E-4 3.000387478611524E-4 0.0 2.02855483050954 2.500322898842936E-4 56-57 1.7145071306351564E-4 3.000387478611524E-4 0.0 2.285588024510594 2.5717606959527347E-4 58-59 1.7145071306351564E-4 3.0718252757213217E-4 0.0 2.5646169162417545 2.5717606959527347E-4 60-61 1.7145071306351564E-4 3.429014261270313E-4 0.0 2.842767123068465 2.5717606959527347E-4 62-63 1.7145071306351564E-4 3.643327652599707E-4 0.0 3.1292755521570212 2.5717606959527347E-4 64-65 1.7145071306351564E-4 4.000516638148698E-4 0.0 3.431007375809676 2.643198493062533E-4 66-67 1.7145071306351564E-4 4.2148300294780926E-4 0.0 3.759121177934979 2.714636290172331E-4 68-69 1.7145071306351564E-4 4.429143420807487E-4 0.0 4.116953103657958 2.786074087282129E-4 70-71 1.8573827248547528E-4 4.643456812136881E-4 0.0 4.522726935021323 2.8575118843919274E-4 72-73 1.8573827248547528E-4 4.7148946092466797E-4 0.0 4.909862645118741 3.14326307283112E-4 74-75 1.8573827248547528E-4 4.7148946092466797E-4 0.0 5.2938408045839065 3.14326307283112E-4 76-77 1.8573827248547528E-4 4.7148946092466797E-4 0.0 5.707487081188771 3.14326307283112E-4 78-79 1.8573827248547528E-4 4.857770203466276E-4 0.0 6.1399786486712 3.2861386670507165E-4 80-81 1.8573827248547528E-4 5.000645797685872E-4 0.0 6.605695935589396 3.571889855489909E-4 82-83 1.8573827248547528E-4 5.143521391905469E-4 0.0 7.106732069398676 3.7862032468193035E-4 84-85 1.8573827248547528E-4 5.143521391905469E-4 0.0 7.598081237919869 4.000516638148698E-4 86-87 1.8573827248547528E-4 5.286396986125065E-4 0.0 8.104589506987761 4.000516638148698E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 2230 0.0 24.605247 1 CTAGGCA 3540 0.0 18.258245 4 GTATAAG 2855 0.0 17.264355 1 TAGGCAT 3545 0.0 17.051973 5 CATGGGG 16225 0.0 16.908878 4 TAGTACT 2840 0.0 16.700497 4 TACTAGG 3425 0.0 16.69906 2 ACTAGGC 3430 0.0 16.403584 3 GTACAAG 3930 0.0 16.328157 1 GTACATG 58015 0.0 15.910024 1 TACATGG 56500 0.0 15.7521925 2 GTATAAT 2440 0.0 15.626965 1 ATACGGG 1325 0.0 15.090377 3 TATACCG 1030 0.0 14.897872 5 ATATACG 2345 0.0 14.8718815 6 GTACATA 1970 0.0 14.634439 1 ACATGGG 53150 0.0 14.627851 3 GTGATCG 1850 0.0 14.578196 8 TACGGGC 1450 0.0 14.1101675 4 CGGGCGT 1420 0.0 14.080811 6 >>END_MODULE