FastQCFastQC Report
Fri 27 May 2016
ERR765503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6999096
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT218530.3122260360480839No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA217180.3102972155261194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191420.27349246245515135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119720.17105066139970077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101900.1455902305097687No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG89320.12761648075694348No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT78030.11148582616955104No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG72080.10298472831348505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG8500.028.4472922
ATCACGC11500.022.2393133
TCACGCA10450.021.8040074
GTATCAA514850.021.6800631
AGTACTT290500.021.14013512-13
GTACTAG23600.020.8980061
TAACGCA5650.018.929364
CAACGCA607650.018.1976325
ATCAACG613850.017.9835323
TACTTTT344800.017.88537214-15
AACGCAG626400.017.8378726
GAGTACT279700.017.79126412-13
TATCAAC628400.017.7595352
TCAACGC629250.017.572974
TCTAACG9800.017.0817722
ACGCAGA670100.016.521937
CGCAGAG675500.016.4105038
CTAGGCA36750.016.1960534
CATGGGG144700.016.132134
TAGGCAT34550.016.016055
Produced by FastQC (version 0.11.5)