##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765503_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6999096 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.403319228654674 33.0 33.0 33.0 33.0 33.0 2 32.297571143473384 33.0 33.0 33.0 33.0 33.0 3 32.351435242494176 33.0 33.0 33.0 33.0 33.0 4 36.26415997151632 37.0 37.0 37.0 33.0 37.0 5 36.20335083273611 37.0 37.0 37.0 33.0 37.0 6 36.22428739368627 37.0 37.0 37.0 33.0 37.0 7 36.12371526265678 37.0 37.0 37.0 33.0 37.0 8 36.13580825295153 37.0 37.0 37.0 33.0 37.0 9 36.15538049485248 37.0 37.0 37.0 33.0 37.0 10-11 36.11134187900838 37.0 37.0 37.0 33.0 37.0 12-13 36.089696797986484 37.0 37.0 37.0 33.0 37.0 14-15 37.60667634791693 40.0 37.0 40.0 33.0 40.0 16-17 37.60327190825787 40.0 37.0 40.0 33.0 40.0 18-19 37.65418398318869 40.0 37.0 40.0 33.0 40.0 20-21 37.644076677902405 40.0 37.0 40.0 33.0 40.0 22-23 37.61704018633263 40.0 37.0 40.0 33.0 40.0 24-25 37.599346044117695 40.0 37.0 40.0 33.0 40.0 26-27 37.48143345940676 40.0 37.0 40.0 33.0 40.0 28-29 37.389573810674975 40.0 37.0 40.0 33.0 40.0 30-31 37.24717527806448 37.0 37.0 40.0 33.0 40.0 32-33 37.13740331608539 37.0 37.0 40.0 33.0 40.0 34-35 37.04850583561077 37.0 37.0 40.0 33.0 40.0 36-37 36.91560638688196 37.0 37.0 40.0 33.0 40.0 38-39 36.8103395781398 37.0 37.0 40.0 33.0 40.0 40-41 36.66160008378225 37.0 37.0 40.0 33.0 40.0 42-43 36.523944020770685 37.0 37.0 40.0 33.0 40.0 44-45 36.260578866185 37.0 37.0 40.0 33.0 40.0 46-47 36.04416677525212 37.0 37.0 40.0 33.0 40.0 48-49 35.79848140388415 37.0 37.0 40.0 30.0 40.0 50-51 35.62292151729309 37.0 37.0 40.0 27.0 40.0 52-53 35.48455193356399 37.0 37.0 40.0 27.0 40.0 54-55 35.307766460125706 37.0 33.0 40.0 27.0 40.0 56-57 35.119257686992725 37.0 33.0 40.0 27.0 40.0 58-59 34.91214465410962 37.0 33.0 40.0 27.0 40.0 60-61 34.747020972422725 37.0 33.0 40.0 27.0 40.0 62-63 34.53518482958371 37.0 33.0 37.0 27.0 40.0 64-65 34.31588186531518 37.0 33.0 37.0 27.0 40.0 66-67 34.08784584466337 37.0 33.0 37.0 27.0 40.0 68-69 33.87465695569828 37.0 33.0 37.0 27.0 40.0 70-71 33.67492980522056 37.0 33.0 37.0 27.0 40.0 72-73 33.455718209894535 37.0 33.0 37.0 27.0 37.0 74-75 33.214894323495486 37.0 33.0 37.0 27.0 37.0 76-77 33.02513267427679 37.0 33.0 37.0 27.0 37.0 78-79 32.8488514088105 37.0 33.0 37.0 27.0 37.0 80-81 32.67807013934371 35.0 33.0 37.0 27.0 37.0 82-83 32.57302443058361 33.0 33.0 37.0 24.5 37.0 84-85 32.44020063448194 33.0 33.0 37.0 22.0 37.0 86-87 32.32416686383499 33.0 33.0 37.0 22.0 37.0 88-89 32.20692186533803 33.0 33.0 37.0 22.0 37.0 90-91 32.100610850315526 33.0 33.0 37.0 22.0 37.0 92-93 32.02211978518369 33.0 33.0 37.0 22.0 37.0 94-95 31.92019597959508 33.0 33.0 37.0 22.0 37.0 96-97 31.863858989789538 33.0 33.0 37.0 22.0 37.0 98-99 31.080214716300503 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0032713870430782777 1101 2 0.07408587814623502 1101 3 0.10679674755281354 1101 4 0.13457041402130443 1101 5 0.05360095056322933 1101 6 0.06274880030746743 1101 7 0.06781224865762425 1101 8 0.09528513853173592 1101 9 0.07521163021080923 1101 10-11 0.07324980638342993 1101 12-13 0.03526953585942749 1101 14-15 0.09032169650922839 1101 16-17 0.10385051389921074 1101 18-19 0.0926880071810885 1101 20-21 0.15494115972984446 1101 22-23 0.07070716070828809 1101 24-25 0.14140921621937252 1101 26-27 0.10064924608976611 1101 28-29 0.03993399228979655 1101 30-31 0.08138285716644589 1101 32-33 0.13052541735221723 1101 34-35 0.16487722378764147 1101 36-37 0.1413989539763847 1101 38-39 0.13588236628005745 1101 40-41 0.02684557942527732 1101 42-43 0.0431811449056454 1101 44-45 0.1071218476752307 1101 46-47 0.1109261742508707 1101 48-49 0.08441545565982977 1101 50-51 0.16852738524248423 1101 52-53 0.1605382434076148 1101 54-55 0.10365026611767547 1101 56-57 0.09481946904497818 1101 58-59 0.15816875654968499 1101 60-61 0.12363734981426688 1101 62-63 0.12185378067082553 1101 64-65 0.07875840137510437 1101 66-67 0.21084212834111327 1101 68-69 0.2138738846657091 1101 70-71 0.09822485525763369 1101 72-73 0.076256445109955 1101 74-75 0.14585509886643422 1101 76-77 0.20831323843652427 1101 78-79 0.15652722508761485 1101 80-81 0.11500105288462947 1101 82-83 0.14870381538099053 1101 84-85 0.15525453903980235 1101 86-87 0.10173177758380803 1101 88-89 0.056273602969646674 1101 90-91 0.12905001026837226 1101 92-93 0.061110270096371266 1101 94-95 0.12183665308297265 1101 96-97 0.046247959206276334 1101 98-99 0.11998608648091391 1102 1 0.01422430538973174 1102 2 0.12880525698299294 1102 3 0.10426703738512799 1102 4 0.09575236286000433 1102 5 0.10003562898376117 1102 6 0.060303721337781724 1102 7 0.029348233278973623 1102 8 0.13593824134721189 1102 9 0.11301839745312492 1102 10-11 0.04965819573831709 1102 12-13 0.08278575335680927 1102 14-15 0.14707467455924927 1102 16-17 0.13197899473662034 1102 18-19 0.14809540249127906 1102 20-21 0.13199449762453241 1102 22-23 0.14698331714808432 1102 24-25 0.22556610806721977 1102 26-27 0.17054017080860007 1102 28-29 0.0759592182163118 1102 30-31 0.1540817054014454 1102 32-33 0.19091447598881928 1102 34-35 0.1678628057543392 1102 36-37 0.16959991258312357 1102 38-39 0.1757282656195045 1102 40-41 0.18159128739486619 1102 42-43 0.1860161703527865 1102 44-45 0.25942244658175184 1102 46-47 0.3003701231193574 1102 48-49 0.2477303196220788 1102 50-51 0.24193206953415114 1102 52-53 0.26211248600782966 1102 54-55 0.1565201441761772 1102 56-57 0.2500176353638679 1102 58-59 0.2468352410854493 1102 60-61 0.132102932972316 1102 62-63 0.30044745193900013 1102 64-65 0.2335570006191503 1102 66-67 0.2970789202252675 1102 68-69 0.24834938292360675 1102 70-71 0.15129875357232692 1102 72-73 0.16021027968995583 1102 74-75 0.33456692725557957 1102 76-77 0.34635745280776575 1102 78-79 0.2508974293412294 1102 80-81 0.23361935068788142 1102 82-83 0.25594872656372303 1102 84-85 0.2329696150039382 1102 86-87 0.2025741963394907 1102 88-89 0.28756878840285083 1102 90-91 0.23178916781935044 1102 92-93 0.2707751210943883 1102 94-95 0.2948220178614136 1102 96-97 0.3085234466443154 1102 98-99 0.26779268537222833 1103 1 -0.02966268079582335 1103 2 0.07281106681899985 1103 3 0.06111681697142757 1103 4 0.05472253307822683 1103 5 0.03518274575178992 1103 6 0.08802307095889716 1103 7 0.029820445171687027 1103 8 0.08773904817409317 1103 9 0.03197092519470601 1103 10-11 0.081491746917564 1103 12-13 0.08201848337984075 1103 14-15 0.13980323679018625 1103 16-17 0.12552423695920822 1103 18-19 0.1171007636860466 1103 20-21 0.10620448374622526 1103 22-23 0.10368343069849573 1103 24-25 0.09758667993320813 1103 26-27 0.0976898284834462 1103 28-29 0.07738107104818681 1103 30-31 0.11704660095827535 1103 32-33 0.11998575838526904 1103 34-35 0.13852554793180616 1103 36-37 0.09616021881883086 1103 38-39 0.03287872150187354 1103 40-41 0.09729498134024084 1103 42-43 0.06325296554122417 1103 44-45 0.08042690213301285 1103 46-47 0.10456974834757915 1103 48-49 0.12213575952619493 1103 50-51 0.14012142148990137 1103 52-53 0.002187747444160948 1103 54-55 5.185080971585876E-4 1103 56-57 0.04876876346715164 1103 58-59 0.10575976290782307 1103 60-61 0.08311256353223229 1103 62-63 0.1058179628118765 1103 64-65 0.1030700715945656 1103 66-67 0.16883331953492586 1103 68-69 0.1727500976498746 1103 70-71 -0.0015140629725252097 1103 72-73 0.06863456993527706 1103 74-75 0.08185603384196583 1103 76-77 0.10105218167772279 1103 78-79 0.09944424214801728 1103 80-81 0.13013483324588293 1103 82-83 -0.018032451678962502 1103 84-85 0.06079452192022927 1103 86-87 0.11178332834344218 1103 88-89 0.13476535253834498 1103 90-91 0.05188389705637775 1103 92-93 0.08619388282215112 1103 94-95 0.0837867809331243 1103 96-97 0.04044281830323371 1103 98-99 0.04727157957219674 1104 1 0.016865334009423805 1104 2 0.04815042194603336 1104 3 -0.05673767449108169 1104 4 -0.024020788885970035 1104 5 -0.03263036992279922 1104 6 0.024073230200428952 1104 7 0.05994025897654609 1104 8 0.061069467604262684 1104 9 -0.034720624240563325 1104 10-11 -0.016182072317306506 1104 12-13 -0.04913365837990824 1104 14-15 -0.018807428913902413 1104 16-17 0.04036158018344338 1104 18-19 0.02610063190559231 1104 20-21 0.04038030137844828 1104 22-23 0.09198003739278704 1104 24-25 0.069776298089117 1104 26-27 0.07237590862069254 1104 28-29 0.04312792448295255 1104 30-31 -0.009741745720901918 1104 32-33 0.014826850395031954 1104 34-35 0.03332458888115042 1104 36-37 0.12612661275726111 1104 38-39 0.031576622181979985 1104 40-41 0.06267130456741654 1104 42-43 0.13807607483960282 1104 44-45 0.0946933638442431 1104 46-47 0.009682911741428768 1104 48-49 0.12264200927553048 1104 50-51 0.00753202359560845 1104 52-53 0.17811945979608623 1104 54-55 0.08408296879179744 1104 56-57 0.1754773563711467 1104 58-59 0.09930502744684588 1104 60-61 0.11845407543872 1104 62-63 0.07389660381982566 1104 64-65 0.030971482218362212 1104 66-67 0.1721011780371029 1104 68-69 0.21098274785298798 1104 70-71 0.044264399354226214 1104 72-73 0.16661701939829499 1104 74-75 0.1635932173744834 1104 76-77 0.20240413861743178 1104 78-79 0.1620300324501187 1104 80-81 0.19747623015141613 1104 82-83 0.15739561993103024 1104 84-85 0.19355140785810931 1104 86-87 0.1567658387856099 1104 88-89 0.1592092963262033 1104 90-91 0.07619273518660208 1104 92-93 0.18841008625187783 1104 94-95 0.1334475997399167 1104 96-97 0.09319715252956229 1104 98-99 0.1968579444698122 1105 1 -0.02736664216428153 1105 2 0.03820016047353647 1105 3 0.026840895806046206 1105 4 0.05898687957437687 1105 5 0.03179088583767964 1105 6 5.727063284766132E-4 1105 7 -0.002701358894917405 1105 8 0.040656523454764226 1105 9 -0.004891548542637736 1105 10-11 0.011848604659398632 1105 12-13 -0.0010595114814719864 1105 14-15 0.02022133864970499 1105 16-17 0.03418783504285727 1105 18-19 -0.0014461996301093905 1105 20-21 -0.01705233138690687 1105 22-23 0.040401278430863385 1105 24-25 0.06810486300166474 1105 26-27 0.007243104421213786 1105 28-29 0.013092377391863863 1105 30-31 0.024375943757029006 1105 32-33 0.02436302741385532 1105 34-35 -0.026360025341610083 1105 36-37 0.058328049363304046 1105 38-39 -0.02930227833306276 1105 40-41 -0.07233781241320969 1105 42-43 -0.041890760916516 1105 44-45 -0.030641604765698105 1105 46-47 -0.09857458761583615 1105 48-49 -0.04356659318458611 1105 50-51 -0.06655569558067498 1105 52-53 -0.12937221591531767 1105 54-55 -0.16533880225257036 1105 56-57 -0.07295946246240703 1105 58-59 -0.034261073116397256 1105 60-61 -0.107450949833364 1105 62-63 -0.11064240521563562 1105 64-65 -0.041887257609893425 1105 66-67 0.03942155661536617 1105 68-69 -0.07829038764403862 1105 70-71 -0.12195964117490377 1105 72-73 -0.07750468874872496 1105 74-75 -0.023031004485488893 1105 76-77 -0.04965590640583173 1105 78-79 -0.01693695830012132 1105 80-81 -0.15061434653305383 1105 82-83 -0.1500009565936793 1105 84-85 -0.13710021701740516 1105 86-87 -0.05705266091489847 1105 88-89 -0.03134132404561285 1105 90-91 -0.07947110382561817 1105 92-93 -0.06422730165683532 1105 94-95 -0.010185655401130589 1105 96-97 -0.10683809943198952 1105 98-99 0.04685202143141254 1106 1 -0.016061567473911964 1106 2 0.03847866043730619 1106 3 -0.0010616750993079904 1106 4 0.047473954825250075 1106 5 0.05131988781231911 1106 6 0.028301037649647753 1106 7 -0.08051470484725343 1106 8 -0.01839431372523137 1106 9 -0.05364803979085053 1106 10-11 -0.02443531530148846 1106 12-13 -0.0192602793127179 1106 14-15 0.004780586865635428 1106 16-17 0.0014828479526727278 1106 18-19 -0.016589152045959565 1106 20-21 -0.03733133990925808 1106 22-23 0.03356955334211165 1106 24-25 0.033662510322770345 1106 26-27 -0.040280027449256295 1106 28-29 -0.0741812775662325 1106 30-31 0.00513662148370031 1106 32-33 0.03652846402155063 1106 34-35 0.049446291536227704 1106 36-37 0.033739488856419086 1106 38-39 -0.029694960850676466 1106 40-41 -0.06583402629259894 1106 42-43 -0.04843325483485472 1106 44-45 -0.02619347349970269 1106 46-47 -0.030223001058907073 1106 48-49 -0.058248585780525275 1106 50-51 -0.0014925825039000529 1106 52-53 -0.05155991374049762 1106 54-55 -0.15395019601733395 1106 56-57 -0.04158117109652437 1106 58-59 -0.014585015883781693 1106 60-61 -0.14576370082413348 1106 62-63 -0.11497790073061509 1106 64-65 -0.05155211347486244 1106 66-67 -0.06897082797713949 1106 68-69 -0.08309764125561969 1106 70-71 -0.15603587526450013 1106 72-73 0.03429215857702417 1106 74-75 -0.015290170134846903 1106 76-77 0.02001121290536645 1106 78-79 -0.03596360147170685 1106 80-81 -0.02307200211383531 1106 82-83 -0.05719398967298872 1106 84-85 -0.04734578793821953 1106 86-87 0.03192844220150448 1106 88-89 0.09194765631637125 1106 90-91 -0.037167911724246494 1106 92-93 0.030269021278442665 1106 94-95 -0.03286011237608477 1106 96-97 0.003353606700084555 1106 98-99 0.04686432878403579 1107 1 -0.05288355373739506 1107 2 0.03824257370514772 1107 3 0.05288421374200425 1107 4 0.017008093633144483 1107 5 -0.051240871034025304 1107 6 -0.01779596947319817 1107 7 -0.018826188670416855 1107 8 0.006205372700264888 1107 9 0.05301948456826011 1107 10-11 -0.03475858880491245 1107 12-13 -0.07708445313177492 1107 14-15 -0.007342292962931651 1107 16-17 -0.059453663057567496 1107 18-19 -0.061707395546733323 1107 20-21 -0.025709249112644272 1107 22-23 -0.019539043824252644 1107 24-25 -0.0357894226318507 1107 26-27 -0.05123365261052726 1107 28-29 -0.0586745695186508 1107 30-31 -0.053831648042873326 1107 32-33 -0.016257896789731774 1107 34-35 -0.08789685990237217 1107 36-37 -0.07829323450582848 1107 38-39 -0.2259856629166137 1107 40-41 -0.21810430019611715 1107 42-43 -0.12508579162442857 1107 44-45 -0.12179320919742054 1107 46-47 -0.057665832520193305 1107 48-49 -0.12102829870081422 1107 50-51 -0.09051036003317847 1107 52-53 -0.12312107047436172 1107 54-55 -0.21759951761384855 1107 56-57 -0.15647408600729307 1107 58-59 -0.16540181315227187 1107 60-61 -0.1348225078323182 1107 62-63 -0.16398542147111073 1107 64-65 -0.1565597149829614 1107 66-67 -0.12472636514964108 1107 68-69 -0.2004620141917215 1107 70-71 -0.12704568271086458 1107 72-73 -0.20035579479202426 1107 74-75 -0.051711231381844414 1107 76-77 -0.12289227612158271 1107 78-79 -0.03950945455352439 1107 80-81 -0.17232799362071916 1107 82-83 -0.15035674628266094 1107 84-85 -0.13261148194835215 1107 86-87 -0.16768510986907614 1107 88-89 -0.16966680960994296 1107 90-91 -0.11567292475590563 1107 92-93 0.018466701370883243 1107 94-95 -0.08623145738569349 1107 96-97 -0.0709230246563699 1107 98-99 -0.08082200223568847 1108 1 -0.08255679649160186 1108 2 -0.034620685525787565 1108 3 0.004983870918358946 1108 4 -0.042984580351131285 1108 5 0.0017506592203204718 1108 6 -0.029043126925721197 1108 7 -0.12806346328598295 1108 8 -0.09518779533263455 1108 9 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-0.08425485987423542 2215 66-67 -0.1718936332107006 2215 68-69 -0.09754528635087212 2215 70-71 -0.17787181687217668 2215 72-73 -0.1894664808224391 2215 74-75 -0.2609724861471463 2215 76-77 -0.15319744235768695 2215 78-79 -0.07487669954272036 2215 80-81 -0.08434030218104027 2215 82-83 -0.059648191968101116 2215 84-85 -0.05761672213166946 2215 86-87 -0.11704752379848316 2215 88-89 -0.05236607488867406 2215 90-91 -0.16385304665107725 2215 92-93 -0.0718495960301091 2215 94-95 -0.06317929591379112 2215 96-97 -0.07161726685538383 2215 98-99 -0.09588580336968278 2216 1 -0.02378107108972216 2216 2 -0.025619857674982427 2216 3 -0.04460457606339929 2216 4 -0.13149958032162345 2216 5 -0.08886043226755191 2216 6 -0.18936427660545263 2216 7 -0.021196851722166343 2216 8 -0.11069650095551964 2216 9 -0.011150681159719511 2216 10-11 -0.09180238198928237 2216 12-13 -0.05565942559636028 2216 14-15 -0.24114965756024276 2216 16-17 -0.16115801350008496 2216 18-19 -0.08196069535718209 2216 20-21 -0.14643576707736372 2216 22-23 -0.19303730543001052 2216 24-25 -0.24409655598014268 2216 26-27 -0.2314380891273018 2216 28-29 -0.14037623412650646 2216 30-31 -0.14205069097236844 2216 32-33 -0.1355215773838978 2216 34-35 -0.18114394950364954 2216 36-37 -0.20514172014240728 2216 38-39 -0.16582815849951515 2216 40-41 -0.21044071004656217 2216 42-43 -0.23338360032730066 2216 44-45 -0.2447020769687427 2216 46-47 -0.25163023300256526 2216 48-49 -0.23990575608322473 2216 50-51 -0.34425005269829967 2216 52-53 -0.33352772246326623 2216 54-55 -0.270076748710089 2216 56-57 -0.3835053155163308 2216 58-59 -0.363647255719151 2216 60-61 -0.3017108151073984 2216 62-63 -0.2709428270115808 2216 64-65 -0.25432857937368425 2216 66-67 -0.28957465579580344 2216 68-69 -0.14529025603166446 2216 70-71 -0.2665505834080264 2216 72-73 -0.22601905336743755 2216 74-75 -0.20383326762620868 2216 76-77 -0.20156910246537763 2216 78-79 -0.11175196761789152 2216 80-81 -0.2502598656922359 2216 82-83 -0.20869915674240502 2216 84-85 -0.22433575661902694 2216 86-87 -0.30148732840967085 2216 88-89 -0.22970598824472077 2216 90-91 -0.21294765933873805 2216 92-93 -0.09916120596990652 2216 94-95 -0.1770746345307117 2216 96-97 -0.17773597654130668 2216 98-99 -0.2921095997307006 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 16.0 10 56.0 11 150.0 12 265.0 13 495.0 14 1209.0 15 3049.0 16 6962.0 17 12944.0 18 20994.0 19 28967.0 20 36921.0 21 47456.0 22 55985.0 23 55997.0 24 49053.0 25 47469.0 26 55017.0 27 70384.0 28 93558.0 29 123704.0 30 163177.0 31 215744.0 32 286939.0 33 379737.0 34 508991.0 35 728235.0 36 1217324.0 37 1953699.0 38 833136.0 39 1457.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.9666751011784 18.75919754913103 13.084129251029653 25.189998098660915 2 15.722130400840337 21.133714982620614 37.56073641510275 25.5834182014363 3 18.240998551812975 26.08732613468939 29.697635237464954 25.97404007603268 4 13.14976962739188 17.374672386262453 36.62551563801954 32.85004234832613 5 13.978696657700256 37.23901276720266 33.65872644602994 15.123564129067141 6 31.51342687684238 36.85135908980246 17.451939507616412 14.183274525738753 7 26.9116754506582 31.950926233902205 22.681929209143583 18.455469106296015 8 27.010688237452378 32.90469226311512 20.454855884245625 19.629763615186878 9 27.2472044961235 14.278886873390507 19.98189480470049 38.492013825785506 10-11 25.23308724440985 25.22136430190413 27.687639946644538 21.857908507041483 12-13 26.494385846400736 23.132330232361436 27.50900973497149 22.86427418626634 14-15 24.00079582712263 23.625399744178015 25.768689346310886 26.60511508238847 16-17 23.162082540069 26.19073592997513 26.66776971755253 23.979411812403338 18-19 23.51935073469619 25.112359899726382 28.90018137462808 22.468107990949342 20-21 24.33716133507499 24.61005424639587 28.276206202741573 22.77657821578757 22-23 23.803888387871805 24.430419299863868 27.99045048103355 23.775241831230776 24-25 23.49536997349372 24.895579371964608 28.01433928038707 23.594711374154606 26-27 23.686573237458095 24.708462349994914 27.926913704284097 23.678050708262894 28-29 23.462444292805813 24.625223028802576 28.43825831221632 23.474074366175287 30-31 23.96332260549405 24.518545617320108 28.149501659468218 23.368630117717622 32-33 23.440327150820618 24.7291435922582 27.91784824783086 23.912681009090317 34-35 23.667849390835617 24.72287849745167 28.242847362002177 23.366424749710536 36-37 24.01303682647016 24.623822847979223 27.770550653970172 23.592589671580445 38-39 24.042276316827202 24.424782857671907 27.772122285506583 23.760818539994307 40-41 23.808789020753537 24.58879689605629 27.88635132308515 23.716062760105018 42-43 24.31966213922484 24.538976176351916 27.363762405887847 23.777599278535398 44-45 24.134366781081443 24.77485663862876 27.076353860555706 24.014422719734092 46-47 23.926604235747018 24.78921563584783 27.234688594069862 24.049491534335292 48-49 24.495177662943902 25.022860095075135 26.581482808637002 23.900479433343964 50-51 23.781356906663376 25.421304408455036 26.338951487449236 24.458387197432355 52-53 23.791565367870366 25.456251778801146 25.949815519032743 24.802367334295745 54-55 23.99902073067722 25.315047829033922 25.97312567222967 24.71280576805919 56-57 24.10882062483498 24.960144852992443 25.951358575450318 24.979675946722264 58-59 23.805140249908003 24.948902326021987 26.537157551286448 24.708799872783562 60-61 23.9782537630574 25.12118707901706 26.25884828555002 24.64171087237552 62-63 23.51360089931614 25.134310202346132 26.273685916009725 25.078402982328 64-65 23.461487026324544 24.947343199750367 26.414961303574064 25.176208470351032 66-67 24.003085541332766 24.988255626155148 26.1587710755789 24.84988775693318 68-69 23.974946193051217 24.636145867980666 26.245332254336844 25.14357568463127 70-71 23.520694672569142 24.7398806931638 26.328521569071206 25.41090306519585 72-73 24.11744316694613 24.74539569110068 26.18328852754699 24.9538726144062 74-75 24.143854956032506 24.707985314951234 26.162266917070593 24.985892811945664 76-77 23.7544846069423 24.867209342712947 26.347453783393405 25.030852266951353 78-79 23.803117876084347 24.90589671530941 26.217916996798802 25.073068411807448 80-81 23.40505025509023 24.942013906946787 26.35522579214561 25.29771004581737 82-83 23.518852834299185 24.96051095759758 26.00121001383965 25.519426194263584 84-85 23.61394273711059 24.991518516637317 25.693954647263517 25.70058409898857 86-87 23.36051728203757 24.71813116776154 26.032210539595795 25.889141010605087 88-89 23.4402676638457 24.4096597015821 26.23617183836013 25.913900796212076 90-91 23.60638039705922 24.556033216881506 26.031244465752156 25.80634192030712 92-93 23.318184467917057 24.706635991738228 25.91882621820539 26.056353322139326 94-95 22.785433179852223 24.747859181051965 26.34953444523733 26.117173193858477 96-97 23.326572326909233 24.493431736552786 26.027074315919595 26.152921620618386 98-99 23.196564047732483 24.529823656769395 25.968947640676067 26.30466465482206 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 135.0 1 156.0 2 489.0 3 834.5 4 588.5 5 505.5 6 1079.5 7 2804.5 8 4999.5 9 4903.0 10 3015.0 11 1925.5 12 1874.5 13 2131.5 14 2534.5 15 3089.0 16 3668.0 17 4336.0 18 5241.5 19 6445.0 20 7977.0 21 9706.5 22 11941.0 23 14888.0 24 18223.5 25 22688.0 26 28445.0 27 34073.5 28 41126.0 29 48647.0 30 55088.0 31 64310.0 32 74694.0 33 84655.0 34 94091.0 35 105204.5 36 115280.5 37 126009.0 38 139135.0 39 142921.0 40 148082.0 41 157106.5 42 166310.5 43 183048.5 44 201148.5 45 220496.0 46 246262.5 47 272664.5 48 295059.0 49 317698.0 50 343799.5 51 333432.5 52 305894.5 53 299438.0 54 285445.5 55 271130.0 56 251942.5 57 233287.5 58 219874.5 59 194618.0 60 162901.5 61 128646.0 62 103710.0 63 82583.0 64 60947.0 65 46255.0 66 34635.5 67 26424.5 68 20829.5 69 17539.0 70 15359.5 71 13348.5 72 12885.0 73 10814.0 74 7342.0 75 5712.5 76 4403.0 77 3029.0 78 1802.0 79 1113.0 80 788.0 81 566.5 82 351.5 83 232.0 84 151.0 85 99.5 86 48.5 87 25.0 88 11.5 89 5.0 90 3.5 91 2.5 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0575217142328095 2 0.0 3 0.0 4 0.0 5 0.00545784769918858 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001264449008843428 16-17 0.004650600591847862 18-19 6.64371513121123E-4 20-21 1.928820521964551E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.3574473046233398E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.143779710979818E-6 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 6.429401739881837E-5 76-77 2.2145717104037437E-4 78-79 6.429401739881837E-5 80-81 5.715023768783854E-5 82-83 3.571889855489909E-5 84-85 3.643327652599707E-4 86-87 7.358093102309213E-4 88-89 2.6431984930625326E-4 90-91 9.215475827163965E-4 92-93 0.0016002066552594791 94-95 0.0011501485334677508 96-97 0.0019716832002304296 98-99 6.64371513121123E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6999096.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.38131818354909 #Duplication Level Percentage of deduplicated Percentage of total 1 73.80839258272357 31.28096970664712 2 14.398280738646918 12.204362345613227 3 4.826360404382197 6.136425478998136 4 2.1336201431563127 3.6170253667974896 5 1.1733746245626606 2.4864581656046285 6 0.7129515463178326 1.8129495800369642 7 0.47244750515744816 1.4016063628771234 8 0.35625244286536467 1.207875850779493 9 0.25778921372598235 0.9832902022087019 >10 1.5601727888971695 12.378184257531519 >50 0.15187593927935467 4.51073363875821 >100 0.12686328688719442 10.992635290288392 >500 0.014198993910530033 4.06698090724659 >1k 0.006981347381535361 4.9126013474909955 >5k 2.6981052672735145E-4 0.7947273418839489 >10k+ 1.686315792045947E-4 1.2131741572374128 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 21853 0.3122260360480839 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 21718 0.3102972155261194 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19142 0.27349246245515135 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11972 0.17105066139970077 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10190 0.1455902305097687 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 8932 0.12761648075694348 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 7803 0.11148582616955104 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 7208 0.10298472831348505 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4287559421959635E-5 2 0.0 0.0 0.0 0.0 1.4287559421959635E-5 3 0.0 0.0 0.0 0.0 1.4287559421959635E-5 4 0.0 0.0 0.0 0.0 1.4287559421959635E-5 5 0.0 0.0 0.0 0.0 1.4287559421959635E-5 6 0.0 0.0 0.0 0.0 2.857511884391927E-5 7 0.0 0.0 0.0 0.0 2.857511884391927E-5 8 0.0 0.0 0.0 1.4287559421959635E-5 2.857511884391927E-5 9 0.0 2.857511884391927E-5 0.0 2.857511884391927E-5 2.857511884391927E-5 10-11 0.0 2.857511884391927E-5 0.0 2.857511884391927E-5 6.429401739881837E-5 12-13 0.0 2.857511884391927E-5 0.0 4.286267826587891E-5 7.143779710979818E-5 14-15 1.4287559421959635E-5 2.857511884391927E-5 0.0 5.000645797685873E-5 7.143779710979818E-5 16-17 2.857511884391927E-5 2.857511884391927E-5 0.0 7.143779710979818E-5 7.143779710979818E-5 18-19 2.857511884391927E-5 4.2862678265878904E-5 0.0 8.572535653175782E-5 7.143779710979818E-5 20-21 2.857511884391927E-5 5.715023768783854E-5 0.0 2.5717606959527347E-4 7.143779710979818E-5 22-23 2.857511884391927E-5 6.429401739881837E-5 0.0 0.001293024127687347 7.143779710979818E-5 24-25 4.286267826587891E-5 7.143779710979818E-5 0.0 0.003443301820692272 7.143779710979818E-5 26-27 5.000645797685873E-5 7.143779710979818E-5 0.0 0.006186513229708522 7.143779710979818E-5 28-29 5.715023768783854E-5 7.143779710979818E-5 0.0 0.01694504547444413 7.143779710979818E-5 30-31 7.143779710979818E-5 7.143779710979818E-5 0.0 0.04715608987217778 7.143779710979818E-5 32-33 9.286913624273764E-5 7.143779710979818E-5 0.0 0.09871989182603011 8.572535653175782E-5 34-35 1.0001291595371745E-4 9.286913624273764E-5 0.0 0.1643783711496456 8.572535653175782E-5 36-37 1.0001291595371745E-4 1.0001291595371745E-4 0.0 0.264062673236658 1.0715669566469728E-4 38-39 1.0001291595371745E-4 1.1430047537567708E-4 0.0 0.41281045437867975 1.2858803479763673E-4 40-41 1.0001291595371745E-4 1.4287559421959637E-4 0.0 0.5934909308287812 1.2858803479763673E-4 42-43 1.0715669566469726E-4 1.4287559421959637E-4 0.0 0.754983214975191 1.2858803479763673E-4 44-45 1.1430047537567708E-4 1.500193739305762E-4 0.0 0.9377996815588756 1.2858803479763673E-4 46-47 1.1430047537567708E-4 1.57163153641556E-4 0.0 1.1394685827998359 1.2858803479763673E-4 48-49 1.2858803479763673E-4 1.57163153641556E-4 0.0 1.3498028888302147 1.2858803479763673E-4 50-51 1.2858803479763673E-4 1.8573827248547528E-4 0.0 1.5780966570539967 1.2858803479763673E-4 52-53 1.2858803479763673E-4 1.8573827248547528E-4 0.0 1.8085264154113618 1.4287559421959637E-4 54-55 1.2858803479763673E-4 1.9288205219645507E-4 0.0 2.0363772692930633 1.57163153641556E-4 56-57 1.2858803479763673E-4 2.000258319074349E-4 0.0 2.295439296732035 1.6430693335253583E-4 58-59 1.2858803479763673E-4 2.000258319074349E-4 0.0 2.576875642225796 1.7145071306351564E-4 60-61 1.3573181450861655E-4 2.1431339132939453E-4 0.0 2.8568903755570716 1.8573827248547528E-4 62-63 1.4287559421959637E-4 2.2860095075135416E-4 0.0 3.1452276122516394 1.8573827248547528E-4 64-65 1.57163153641556E-4 2.428885101733138E-4 0.0 3.4494740463625586 1.9288205219645507E-4 66-67 1.57163153641556E-4 2.428885101733138E-4 0.0 3.780688248882427 2.000258319074349E-4 68-69 1.57163153641556E-4 2.500322898842936E-4 0.0 4.1411419417593365 2.1431339132939453E-4 70-71 1.57163153641556E-4 2.5717606959527347E-4 0.0 4.549523252717208 2.1431339132939453E-4 72-73 1.57163153641556E-4 2.5717606959527347E-4 0.0 4.939530762258441 2.1431339132939453E-4 74-75 1.57163153641556E-4 2.714636290172331E-4 0.0 5.326937935984876 2.2860095075135416E-4 76-77 1.57163153641556E-4 2.714636290172331E-4 0.0 5.744384703395982 2.5717606959527347E-4 78-79 1.57163153641556E-4 3.14326307283112E-4 0.0 6.18124112028182 2.714636290172331E-4 80-81 1.6430693335253583E-4 3.14326307283112E-4 0.0 6.651601864012153 2.714636290172331E-4 82-83 1.7145071306351564E-4 3.14326307283112E-4 0.0 7.157052853682819 2.786074087282129E-4 84-85 1.7145071306351564E-4 3.14326307283112E-4 0.0 7.654095614633661 2.8575118843919274E-4 86-87 1.7145071306351564E-4 3.14326307283112E-4 0.0 8.166590371099353 2.8575118843919274E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 850 0.0 28.447292 2 ATCACGC 1150 0.0 22.239313 3 TCACGCA 1045 0.0 21.804007 4 GTATCAA 51485 0.0 21.680063 1 AGTACTT 29050 0.0 21.140135 12-13 GTACTAG 2360 0.0 20.898006 1 TAACGCA 565 0.0 18.92936 4 CAACGCA 60765 0.0 18.197632 5 ATCAACG 61385 0.0 17.983532 3 TACTTTT 34480 0.0 17.885372 14-15 AACGCAG 62640 0.0 17.837872 6 GAGTACT 27970 0.0 17.791264 12-13 TATCAAC 62840 0.0 17.759535 2 TCAACGC 62925 0.0 17.57297 4 TCTAACG 980 0.0 17.081772 2 ACGCAGA 67010 0.0 16.52193 7 CGCAGAG 67550 0.0 16.410503 8 CTAGGCA 3675 0.0 16.196053 4 CATGGGG 14470 0.0 16.13213 4 TAGGCAT 3455 0.0 16.01605 5 >>END_MODULE