FastQCFastQC Report
Fri 27 May 2016
ERR765502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11535038
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT777930.6744061007861439No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA745590.6463697822235176No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG455240.3946584311209031No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG341840.29634926213507057No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA279310.24214051137066042No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC273430.23704299890472835No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG269250.23341925704969502No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC261390.22660523528401033No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC241180.2090847034920908No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT235160.20386582168173176No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC206130.17869902119091416No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT194170.16833061148129724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175530.1521711501947371No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG141380.12256569939344804No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA131690.1141652069113253No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC126060.10928442541758424No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA120700.10463771337380944No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC117980.10227968039637148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGC10000.025.390229
TCTACAC113950.025.1581753
TACACTC111000.024.3115485
CTACACT150200.023.6576714
TAGTACT63100.023.3894714
GTGATCG41250.022.3411128
CTGTGCG67100.022.1430429
ATCTACA115850.022.1080742
CGTTAAC17350.021.9410151
CTATTGA67400.021.904969
GTATAAG56250.021.8902111
GTACAAA43700.021.616521
GTACTAG57950.021.5724431
ATACGGG46350.020.8872433
TACGGGC46650.020.6548634
CTAGTAC79150.020.4254263
GAACAAA77000.020.1416191
CTAACGC36400.020.141273
TCTAACG37350.020.131672
AGGGCTA78250.020.0671585
Produced by FastQC (version 0.11.5)