##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765502_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11535038
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.738997825581503	33.0	33.0	33.0	27.0	33.0
2	31.72774012534679	33.0	33.0	33.0	27.0	33.0
3	31.71023667195548	33.0	33.0	33.0	27.0	33.0
4	35.41226929638203	37.0	37.0	37.0	33.0	37.0
5	35.478306616761905	37.0	37.0	37.0	33.0	37.0
6	35.425591835935	37.0	37.0	37.0	33.0	37.0
7	35.35667563470532	37.0	37.0	37.0	33.0	37.0
8	35.345993051778414	37.0	37.0	37.0	33.0	37.0
9	35.343276372388196	37.0	37.0	37.0	33.0	37.0
10-11	35.305749317860936	37.0	37.0	37.0	33.0	37.0
12-13	35.28301605941827	37.0	37.0	37.0	33.0	37.0
14-15	36.760720944308986	37.0	37.0	40.0	33.0	40.0
16-17	36.79739654953889	37.0	37.0	40.0	33.0	40.0
18-19	36.84779157207805	38.5	37.0	40.0	33.0	40.0
20-21	36.8638996681242	37.0	37.0	40.0	33.0	40.0
22-23	36.85154808332665	37.0	37.0	40.0	33.0	40.0
24-25	36.82112945791769	37.0	37.0	40.0	33.0	40.0
26-27	36.80060109034751	37.0	37.0	40.0	33.0	40.0
28-29	36.76124486976116	37.0	37.0	40.0	33.0	40.0
30-31	36.65053431120036	37.0	37.0	40.0	33.0	40.0
32-33	36.51628525194282	37.0	37.0	40.0	33.0	40.0
34-35	36.40374136608826	37.0	37.0	40.0	33.0	40.0
36-37	36.33556066308581	37.0	37.0	40.0	33.0	40.0
38-39	36.22158687296912	37.0	37.0	40.0	33.0	40.0
40-41	36.05432530868126	37.0	37.0	40.0	30.0	40.0
42-43	35.93344512605853	37.0	37.0	40.0	27.0	40.0
44-45	35.755608260675	37.0	37.0	40.0	27.0	40.0
46-47	35.5234593938919	37.0	37.0	40.0	27.0	40.0
48-49	35.27403741539473	37.0	33.0	40.0	27.0	40.0
50-51	35.05810383979663	37.0	33.0	40.0	27.0	40.0
52-53	34.8827132602424	37.0	33.0	40.0	27.0	40.0
54-55	34.75116193808811	37.0	33.0	40.0	27.0	40.0
56-57	34.58148984858134	37.0	33.0	40.0	27.0	40.0
58-59	34.39832166135907	37.0	33.0	38.5	27.0	40.0
60-61	34.11579493712981	37.0	33.0	37.0	27.0	40.0
62-63	33.88870942601143	37.0	33.0	37.0	27.0	40.0
64-65	33.67349440027853	37.0	33.0	37.0	27.0	40.0
66-67	33.4537717170936	37.0	33.0	37.0	27.0	40.0
68-69	33.21286938976708	37.0	33.0	37.0	22.0	40.0
70-71	32.98223820328984	37.0	33.0	37.0	22.0	38.5
72-73	32.70927100543579	37.0	33.0	37.0	22.0	37.0
74-75	32.51412470422724	35.0	33.0	37.0	22.0	37.0
76-77	32.301296580037274	33.0	33.0	37.0	22.0	37.0
78-79	32.09036701916369	33.0	33.0	37.0	22.0	37.0
80-81	31.920020679602445	33.0	33.0	37.0	22.0	37.0
82-83	31.732926800934685	33.0	33.0	37.0	22.0	37.0
84-85	31.563603171485	33.0	33.0	37.0	22.0	37.0
86-87	31.41615424240475	33.0	33.0	37.0	22.0	37.0
88-89	31.29153120258468	33.0	33.0	37.0	22.0	37.0
90-91	31.150570375234132	33.0	30.0	37.0	22.0	37.0
92-93	30.965804403938677	33.0	27.0	37.0	15.0	37.0
94-95	30.87759554844986	33.0	27.0	37.0	15.0	37.0
96-97	30.696869659207017	33.0	27.0	37.0	15.0	37.0
98-99	30.56495730659925	33.0	27.0	37.0	15.0	37.0
100	30.46774739710437	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	7.0
7	259.0
8	1648.0
9	2459.0
10	3088.0
11	7268.0
12	17966.0
13	31268.0
14	36442.0
15	37276.0
16	39668.0
17	45020.0
18	54735.0
19	64536.0
20	77816.0
21	97629.0
22	113081.0
23	108005.0
24	102270.0
25	103794.0
26	114660.0
27	134305.0
28	164432.0
29	208013.0
30	268749.0
31	354189.0
32	478979.0
33	658642.0
34	933264.0
35	1413947.0
36	2233124.0
37	2761466.0
38	866478.0
39	554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95012384602945	19.727092169800645	12.766290641246256	24.556493342923652
2	15.213056658794896	21.739773497986516	37.83139697742427	25.215772865794317
3	18.1477353890854	27.385467211308256	29.616681056075695	24.850116343530654
4	12.702669522443466	18.014282583183284	36.9948607808811	32.28818711349216
5	12.964854330918246	38.26396871408949	34.06159348604399	14.70958346894827
6	30.23111613495399	37.94373509829049	17.539240030479444	14.28590873627608
7	25.268162945967205	33.26084928496817	22.734920451061694	18.736067318002934
8	26.316844931956553	33.63141078133422	20.575233610949585	19.476510675759645
9	26.790633226670202	14.885342043944009	20.07232967335557	38.25169505603022
10-11	24.431349052342004	26.542579605322693	26.949304391180394	22.076766951154912
12-13	25.988511401749992	24.46439598574596	26.883156874839738	22.663935737664318
14-15	24.16113285945022	24.366247049754865	25.18753182826956	26.285088262525353
16-17	22.66198971049413	27.200729459513763	26.55374377929645	23.583537050695657
18-19	23.25551150299089	26.83693501198575	28.415756753082444	21.491796731940916
20-21	24.213534957826035	26.031115120605904	27.212782801879733	22.542567119688332
22-23	23.699466113314642	26.14355122725277	26.13548883089358	24.02149382853901
24-25	23.408128676408914	26.602948856044833	26.20508099603034	23.783841471515917
26-27	23.842373991478855	25.976388045429346	26.325595467988922	23.855642495102877
28-29	23.136434135879256	25.723609719741287	27.694685227100667	23.44527091727879
30-31	24.204260233535134	25.70788150618199	27.15112691708536	22.936731343197515
32-33	22.814151537199635	26.538402742063887	26.870928887234452	23.77651683350203
34-35	22.852774326181564	26.544288266218235	27.5250266253608	23.077910782239403
36-37	23.720937954918277	26.203578747145883	26.744288423231175	23.33119487470466
38-39	24.034854281455562	25.35922074212084	26.855431069442183	23.75049390698141
40-41	23.185266119841838	25.669297264588277	27.333403304576194	23.81203331099369
42-43	24.20226027429623	25.56837060338642	26.810193718909503	23.41917540340785
44-45	23.8592879199862	25.72781598397269	27.057693268850596	23.35520282719051
46-47	23.070184515887213	25.732250268576195	27.558572716681844	23.638992498854748
48-49	23.95599115029822	25.914676125401066	27.189877191138784	22.939455533161933
50-51	22.99822985432994	26.365785252554623	26.264511453406904	24.371473439708538
52-53	22.430256263558913	26.673054966810874	25.627764947898303	25.268923821731914
54-55	22.969479084191725	26.3315106086272	25.54579091246061	25.153219394720466
56-57	23.615513615691793	25.278228631529977	25.740092950318793	25.36616480245944
58-59	22.947746239607124	25.215567477903967	27.117100116557406	24.7195861659315
60-61	23.1979068551223	25.86552126784458	26.195379898373787	24.741191978659327
62-63	22.38529798460907	25.78496823285671	26.194134050057045	25.635599732477175
64-65	21.85681536424608	25.840677089984894	26.402848044992748	25.899659500776274
66-67	23.244121826368914	25.540470635404272	25.856663190266822	25.358744347959995
68-69	23.095896831326662	24.996480080288848	26.14161573331285	25.76600735507164
70-71	22.435244455963613	25.334693438933986	26.23298475425485	25.997077350847555
72-73	23.47690990222663	25.01491598861759	26.403131432066264	25.10504267708951
74-75	23.38589415514333	25.068764626056282	26.45871733978278	25.086623879017605
76-77	22.578383512509063	25.428268221281126	26.660187179719497	25.333161086490314
78-79	22.705399050094265	25.64095272851577	26.538707234724786	25.114940986665175
80-81	22.33820032130617	25.657845341277262	26.66382583228789	25.34012850512867
82-83	22.581914390145936	25.307042148999848	26.49356292345167	25.617480537402542
84-85	22.859481033476666	25.427094171290417	25.865245849462976	25.848178945769945
86-87	22.458473702081168	25.080768607730008	26.433523310224842	26.027234379963982
88-89	22.826436746134597	24.31008408927216	26.747161046439654	26.116318118153593
90-91	22.936763668619932	24.709654744805498	26.397412788056545	25.956168798518025
92-93	22.685015653196732	24.862718100976643	26.4961303938336	25.95613585199303
94-95	21.777161665012958	25.02357454928331	26.955023045709524	26.2442407399942
96-97	22.501842119829877	25.030813975741278	26.237729188900737	26.229614715528104
98-99	22.338491370057735	25.05722835474679	26.13434991328617	26.469930361909306
100	22.370298070425708	25.000307778899266	26.14976226464234	26.479631886032685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	504.0
1	443.5
2	374.5
3	378.5
4	327.0
5	286.5
6	350.5
7	669.5
8	1059.0
9	1057.5
10	862.5
11	827.0
12	1014.5
13	1272.0
14	1608.0
15	2166.0
16	2826.0
17	3565.5
18	4546.5
19	5632.0
20	7095.0
21	8996.0
22	11203.5
23	14155.5
24	17843.5
25	22616.0
26	29370.5
27	37676.5
28	47623.5
29	63315.0
30	81850.0
31	98352.0
32	120056.5
33	143784.0
34	168373.0
35	188981.5
36	207473.5
37	237301.5
38	254014.0
39	259432.0
40	262535.0
41	264701.5
42	274691.5
43	298988.5
44	335846.5
45	377943.5
46	428861.5
47	487613.5
48	570570.0
49	637648.5
50	701757.5
51	701173.0
52	598466.5
53	521610.5
54	464082.5
55	397147.0
56	351966.5
57	302666.0
58	258855.5
59	235750.5
60	198095.5
61	157052.5
62	125829.5
63	100991.5
64	78774.0
65	60903.5
66	49044.5
67	39616.0
68	32590.0
69	27699.0
70	23778.5
71	20667.5
72	18502.5
73	17740.0
74	14404.5
75	11133.5
76	8991.0
77	6955.0
78	5295.5
79	3672.0
80	2587.5
81	2037.0
82	1801.0
83	1562.0
84	1173.0
85	789.0
86	583.0
87	335.0
88	158.5
89	102.0
90	75.5
91	53.5
92	36.5
93	30.0
94	23.5
95	16.5
96	11.0
97	8.0
98	3.5
99	1.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022305951657896575
2	0.004950135404842186
3	0.009466808865302394
4	0.00858254649876316
5	0.010021640154111326
6	0.006129151893561165
7	0.008721254320965393
8	0.00876460051540359
9	0.007854330432201437
10-11	0.02740779874327245
12-13	0.029744158623491317
14-15	0.01267876187317285
16-17	0.004078876896634411
18-19	0.011634118587212283
20-21	0.0016991708219773528
22-23	5.2015433325837326E-5
24-25	0.0037234381022411885
26-27	0.0017598554941908296
28-29	0.0012353665414886366
30-31	0.026974336798890476
32-33	0.020901534958098968
34-35	0.007993038254403671
36-37	0.008924981434824921
38-39	0.006332879007420695
40-41	0.01498477941728497
42-43	0.004026861463308573
44-45	0.004139561568847888
46-47	0.013285608595307618
48-49	0.017286462341953274
50-51	0.008313800093246334
52-53	0.02164708950243597
54-55	0.027104375382205066
56-57	0.014499302039577154
58-59	0.010719513884566309
60-61	0.008721254320965393
62-63	0.015747672439397252
64-65	0.009458139626414754
66-67	0.017633231897458853
68-69	0.005929759399145456
70-71	0.005543978268645495
72-73	0.011040275723408974
74-75	0.0034763647939434613
76-77	0.010429094381830385
78-79	0.0027264756301626403
80-81	0.0075248993544711346
82-83	0.0019159017941683418
84-85	0.008829619807060887
86-87	0.0033766685467356066
88-89	0.0013350627886964915
90-91	0.0013003858331459334
92-93	1.2136934442695378E-4
94-95	0.003060241327336763
96-97	8.105738359942985E-4
98-99	0.004733404432651197
100	0.006796683287909411
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1535038E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.562919429853224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.32707373018734	24.946335870630378
2	14.011865797705832	9.405582456606338
3	5.057392569556069	5.092225780114461
4	2.200640208457728	2.954396400422485
5	1.1768157181459895	1.974868556595935
6	0.6528922873927417	1.3147782742881098
7	0.42765246523171224	1.0047285664185044
8	0.30487514971057234	0.8186000068720303
9	0.22240082688012217	0.6717978930339247
>10	1.2827969874591134	8.106061489361249
>50	0.1390147515924545	3.2692337007328565
>100	0.14165066115183028	10.41887626516723
>500	0.028272875444889968	6.6190331897669745
>1k	0.02465352260336684	15.362339077469978
>5k	0.0014563261675517086	3.325347941865606
>10k+	5.461223128318907E-4	4.715794530654155
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	77793	0.6744061007861439	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	74559	0.6463697822235176	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	45524	0.3946584311209031	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	34184	0.29634926213507057	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	27931	0.24214051137066042	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	27343	0.23704299890472835	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	26925	0.23341925704969502	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	26139	0.22660523528401033	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	24118	0.2090847034920908	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	23516	0.20386582168173176	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	20613	0.17869902119091416	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	19417	0.16833061148129724	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17553	0.1521711501947371	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	14138	0.12256569939344804	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	13169	0.1141652069113253	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	12606	0.10928442541758424	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	12070	0.10463771337380944	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	11798	0.10227968039637148	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.415236776853271E-4	0.0	0.0	2.6007716662918666E-5	0.0
2	0.0012050242053818982	0.0	0.0	4.3346194438197776E-5	0.0
3	0.001248370399820096	0.0	0.0	4.3346194438197776E-5	0.0
4	0.001421755177572887	8.669238887639555E-6	0.0	5.201543332583733E-5	0.0
5	0.004941466165954546	2.6007716662918666E-5	0.0	1.56046299977512E-4	8.669238887639555E-6
6	0.010836548609549444	2.6007716662918666E-5	0.0	3.467695555055822E-4	2.6007716662918666E-5
7	0.01226697302600997	2.6007716662918666E-5	0.0	3.6410803328086133E-4	3.467695555055822E-5
8	0.014408275031256941	2.6007716662918666E-5	0.0	4.1612346660669866E-4	3.467695555055822E-5
9	0.016090107375459014	2.6007716662918666E-5	0.0	4.5946966104489645E-4	3.467695555055822E-5
10-11	0.03219321860924949	4.3346194438197776E-5	0.0	8.105738359942985E-4	4.3346194438197776E-5
12-13	0.06281730497983622	4.3346194438197776E-5	0.0	0.001382743602578509	1.1703472498313401E-4
14-15	0.10041145941608515	4.3346194438197776E-5	0.0	0.002011263421932377	1.5604629997751198E-4
16-17	0.11744217921085306	4.3346194438197776E-5	0.0	0.002401379171876157	1.8638863608425044E-4
18-19	0.12722541529555428	4.3346194438197776E-5	0.0	0.002665790957949163	2.0806173330334933E-4
20-21	0.1631984220598146	4.3346194438197776E-5	0.0	0.0035153763689378397	2.2540021107862843E-4
22-23	0.17396561675826294	4.3346194438197776E-5	0.0	0.004772416007645575	2.514079277415471E-4
24-25	0.22294681647342646	4.3346194438197776E-5	0.0	0.00934110490143162	2.774156444044658E-4
26-27	0.34779252569432373	4.3346194438197776E-5	0.0	0.022730744363390912	3.12092599955024E-4
28-29	0.37253453347964693	4.3346194438197776E-5	0.0	0.04993915061224766	3.164272193988438E-4
30-31	0.38079198352012367	4.3346194438197776E-5	0.0	0.12863850123423953	3.2076183884266354E-4
32-33	0.3881261596190667	4.3346194438197776E-5	0.0	0.26434676678134916	3.294310777303031E-4
34-35	0.39321933746555493	4.768081388201756E-5	0.0	0.4484597276575942	3.467695555055822E-4
36-37	0.4023783883503461	6.501929165729667E-5	0.0	0.7339160911303457	3.467695555055822E-4
38-39	0.4111343196268621	7.8023149988756E-5	0.0	1.1862553031901586	3.5977341383704154E-4
40-41	0.4194567889589961	7.8023149988756E-5	0.0	1.8154946693717005	3.6410803328086133E-4
42-43	0.4431844957944655	7.8023149988756E-5	0.0	2.276117339188653	3.6410803328086133E-4
44-45	0.47016750183224365	7.8023149988756E-5	0.0	2.743198591976897	3.9878498883141953E-4
46-47	0.4797513454225292	7.8023149988756E-5	0.0	3.2914846054256603	4.2045808605051845E-4
48-49	0.4871765485297924	7.8023149988756E-5	0.0	3.827828742306701	4.247927054943382E-4
50-51	0.49987264888074057	7.8023149988756E-5	0.0	4.436300946732902	4.334619443819778E-4
52-53	0.5036914486107458	7.8023149988756E-5	0.0	5.099827152715058	4.3779656382579753E-4
54-55	0.5167386531366434	1.2136934442695378E-4	0.0	5.686175459500003	4.5946966104489645E-4
56-57	0.5448746679464775	1.3870782220223288E-4	0.0	6.302887775488906	4.984812360392744E-4
58-59	0.5528763754397688	1.4737706108987244E-4	0.0	6.975958813486354	5.418274304774722E-4
60-61	0.5584246883278581	1.56046299977512E-4	0.0	7.613347264222277	5.548312888089315E-4
62-63	0.5634745199799082	1.56046299977512E-4	0.0	8.270826676080304	5.981774832471294E-4
64-65	0.5672629773738067	1.6038091942133177E-4	0.0	8.951270901751688	6.371890582415073E-4
66-67	0.5724298437508398	1.6471553886515154E-4	0.0	9.636010735291899	6.545275360167864E-4
68-69	0.5770245403612888	1.6471553886515154E-4	0.0	10.422163325339717	6.762006332358853E-4
70-71	0.5830713344854174	1.733847777527911E-4	0.0	11.350868545036436	6.805352526797051E-4
72-73	0.5993564997358483	1.8205401664043067E-4	0.0	12.16110861533356	7.108775887864435E-4
74-75	0.6153425762446556	1.8205401664043067E-4	0.0	12.932042356514128	7.498891637808215E-4
76-77	0.6200629768189754	1.8205401664043067E-4	0.0	13.773812448645597	7.715622609999204E-4
78-79	0.623630368621239	1.8205401664043067E-4	0.0	14.61141263687211	8.062392165504785E-4
80-81	0.6305137442980249	1.907232555280702E-4	0.0	15.538449028082958	8.409161721010369E-4
82-83	0.632676719400491	1.907232555280702E-4	0.0	16.56678981031532	8.712585082077753E-4
84-85	0.6415540200214338	1.907232555280702E-4	0.0	17.52362671020243	8.799277470954148E-4
86-87	0.6577178159274377	1.907232555280702E-4	0.0	18.46635008918046	9.016008443145137E-4
88	0.661055472899179	1.9939249441570977E-4	0.0	19.202806267304883	9.276085609774324E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCGC	1000	0.0	25.39022	9
TCTACAC	11395	0.0	25.158175	3
TACACTC	11100	0.0	24.311548	5
CTACACT	15020	0.0	23.657671	4
TAGTACT	6310	0.0	23.389471	4
GTGATCG	4125	0.0	22.341112	8
CTGTGCG	6710	0.0	22.143042	9
ATCTACA	11585	0.0	22.108074	2
CGTTAAC	1735	0.0	21.941015	1
CTATTGA	6740	0.0	21.90496	9
GTATAAG	5625	0.0	21.890211	1
GTACAAA	4370	0.0	21.61652	1
GTACTAG	5795	0.0	21.572443	1
ATACGGG	4635	0.0	20.887243	3
TACGGGC	4665	0.0	20.654863	4
CTAGTAC	7915	0.0	20.425426	3
GAACAAA	7700	0.0	20.141619	1
CTAACGC	3640	0.0	20.14127	3
TCTAACG	3735	0.0	20.13167	2
AGGGCTA	7825	0.0	20.067158	5
>>END_MODULE