FastQCFastQC Report
Fri 27 May 2016
ERR765501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11745124
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT870960.7415502807803477No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA867730.7388002033865287No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG347700.2960377429816833No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC251090.21378233214055467No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC250610.21337365190865587No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG249700.21259886230234776No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG239770.2041442900049416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233320.1986526493888017No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT208700.1776907591609931No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC173460.14768681880242387No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA172900.14721002519854196No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC158450.13490704738408893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143210.12193145002130246No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG142180.12105449035701964No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT141900.12081609355507868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA32600.029.1083641
TACATGA40250.026.6102712
GTACAAG54750.025.3117371
GTACTAG57650.023.305091
GTACAAA51450.023.2829931
TAGGCAT69400.022.4028195
GTATAAG53100.021.8517631
GTGATCG39450.020.5971588
TAGTACT58100.020.4557534
CTAGGCA83650.019.9357914
CTATTGA58050.019.8231249
GTCCTAC50050.019.5228541
TCTAACG26500.019.3223952
CGTTAAC16750.019.0712051
CTAGTAC71500.018.7248063
CTGTGCG66300.018.6316139
CATGGGG333100.018.5730084
CTAACGC26450.018.470823
TGATCGC45500.018.3745569
AGGGCTA64700.018.2223155
Produced by FastQC (version 0.11.5)