##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765501_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11745124
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73584391275903	33.0	33.0	33.0	27.0	33.0
2	31.630406711755448	33.0	33.0	33.0	27.0	33.0
3	31.645451848784226	33.0	33.0	33.0	27.0	33.0
4	35.34348415563769	37.0	37.0	37.0	33.0	37.0
5	35.44752835304251	37.0	37.0	37.0	33.0	37.0
6	35.43519787445411	37.0	37.0	37.0	33.0	37.0
7	35.25125430774507	37.0	37.0	37.0	33.0	37.0
8	35.206322044790674	37.0	37.0	37.0	33.0	37.0
9	35.018729133894205	37.0	37.0	37.0	33.0	37.0
10-11	35.042073417019694	37.0	37.0	37.0	33.0	37.0
12-13	34.99168442155229	37.0	37.0	37.0	33.0	37.0
14-15	36.56001567118406	40.0	37.0	40.0	33.0	40.0
16-17	36.631082140980375	40.0	37.0	40.0	33.0	40.0
18-19	36.60459766112303	40.0	37.0	40.0	33.0	40.0
20-21	36.33634204287668	40.0	37.0	40.0	27.0	40.0
22-23	36.415798462408745	40.0	37.0	40.0	33.0	40.0
24-25	36.32572363646395	37.0	37.0	40.0	27.0	40.0
26-27	36.292133143932745	37.0	37.0	40.0	30.0	40.0
28-29	36.2223729608985	37.0	37.0	40.0	27.0	40.0
30-31	36.305586258603995	37.0	37.0	40.0	33.0	40.0
32-33	36.039185112051605	37.0	37.0	40.0	27.0	40.0
34-35	35.85406450370384	37.0	37.0	40.0	27.0	40.0
36-37	35.94093527663054	37.0	37.0	40.0	27.0	40.0
38-39	35.83464559420573	37.0	37.0	40.0	27.0	40.0
40-41	35.83296519474805	37.0	37.0	40.0	27.0	40.0
42-43	35.6818711747956	37.0	37.0	40.0	27.0	40.0
44-45	35.5581671168393	37.0	37.0	40.0	27.0	40.0
46-47	35.3077871719362	37.0	33.0	40.0	27.0	40.0
48-49	35.02359340778352	37.0	33.0	40.0	27.0	40.0
50-51	34.82077349715507	37.0	33.0	40.0	27.0	40.0
52-53	34.68087820954466	37.0	33.0	40.0	27.0	40.0
54-55	34.54375177307622	37.0	33.0	40.0	27.0	40.0
56-57	34.37251249965517	37.0	33.0	40.0	27.0	40.0
58-59	34.19815193096301	37.0	33.0	38.5	27.0	40.0
60-61	33.95529719396747	37.0	33.0	37.0	27.0	40.0
62-63	33.69950964332092	37.0	33.0	37.0	22.0	40.0
64-65	33.52295986828236	37.0	33.0	37.0	22.0	40.0
66-67	33.25276229522992	37.0	33.0	37.0	22.0	40.0
68-69	33.05275397688437	37.0	33.0	37.0	22.0	40.0
70-71	32.754609742732384	37.0	33.0	37.0	22.0	37.0
72-73	32.516175776432846	37.0	33.0	37.0	22.0	37.0
74-75	32.32045579084563	37.0	33.0	37.0	22.0	37.0
76-77	32.04306680797921	33.0	33.0	37.0	22.0	37.0
78-79	31.963427972322812	33.0	33.0	37.0	22.0	37.0
80-81	31.82374051563866	33.0	33.0	37.0	22.0	37.0
82-83	31.68085611526962	33.0	33.0	37.0	22.0	37.0
84-85	31.513375124860325	33.0	33.0	37.0	22.0	37.0
86-87	31.333998517171892	33.0	33.0	37.0	18.5	37.0
88-89	31.193513495472672	33.0	33.0	37.0	15.0	37.0
90-91	31.066156687660342	33.0	27.0	37.0	15.0	37.0
92-93	30.913204620061908	33.0	27.0	37.0	15.0	37.0
94-95	30.788015179746083	33.0	27.0	37.0	15.0	37.0
96-97	30.672154802282208	33.0	27.0	37.0	15.0	37.0
98-99	30.553679765322187	33.0	27.0	37.0	15.0	37.0
100	30.323660695280868	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	18.0
7	298.0
8	1521.0
9	3054.0
10	4808.0
11	8630.0
12	16896.0
13	30952.0
14	46918.0
15	53930.0
16	58251.0
17	66390.0
18	75709.0
19	85879.0
20	96329.0
21	110914.0
22	127188.0
23	123968.0
24	115537.0
25	117237.0
26	130088.0
27	151505.0
28	183948.0
29	229345.0
30	287039.0
31	363138.0
32	473626.0
33	634225.0
34	860298.0
35	1228915.0
36	2013943.0
37	2950260.0
38	1093251.0
39	1114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.512341989947785	19.175281242181565	12.919836547168792	24.392540220701857
2	15.264491296868957	21.587141916390067	37.71365299903937	25.434713787701607
3	17.825012399975307	27.948995123031175	29.23103123090776	24.994961246085758
4	12.432454065017632	18.161723665057483	36.74663201648155	32.65919025344334
5	13.168402360857481	38.527752883200776	33.19212402574775	15.111720730193994
6	30.348688244839394	37.45218885269083	17.876835112782455	14.32228778968732
7	26.117108458277826	32.99704813678005	22.64078749054496	18.24505591439716
8	27.31827467633407	32.495235098015975	20.18411118059217	20.002379045057786
9	27.633218396108898	14.549055160842581	19.773746394411493	38.043980048637025
10-11	24.987583102325324	26.071743125538443	26.177463957578507	22.763209814557726
12-13	26.42336399708881	24.259712279953696	26.226961740746805	23.089961982210685
14-15	24.70413953690932	24.33967873111173	24.630265683208837	26.32591604877011
16-17	23.12553988452181	26.60974508404159	26.237773668318194	24.026941363118407
18-19	23.77529126545697	25.761971857788442	28.37643553074369	22.086301346010895
20-21	25.05261441511937	24.945840168148152	27.28719334866262	22.71435206806986
22-23	24.0899381351527	25.070439529807913	26.497916926294263	24.341705408745124
24-25	23.474039967492423	25.906853851222223	26.611167662607933	24.00793851867742
26-27	24.17463220147688	25.167700456289683	26.665904445093112	23.99176289714033
28-29	23.385494290306845	25.08361262406901	27.79172137716032	23.739171708463825
30-31	24.22363684400324	25.300736068551423	27.145447084437084	23.330180003008255
32-33	23.101209194240436	25.642266530357528	26.755386847019906	24.501137428382126
34-35	23.368024833245066	25.85151118098673	27.60769196861219	23.172772017156014
36-37	24.130044968332054	25.50427324364652	26.775952700203792	23.58972908781763
38-39	24.44646777130773	24.83790741209708	26.63314292879204	24.082481887803148
40-41	23.546929098606697	25.6158659490827	27.103195397395734	23.734009554914866
42-43	24.70248667169933	25.248715724762604	26.445494307755503	23.603303295782563
44-45	24.208074890613243	25.616643524491828	26.17024994528373	24.0050316396112
46-47	23.42159419820747	25.520472204854926	27.166834755558433	23.89109884137917
48-49	24.546246354195063	25.782825873630266	26.23232040151556	23.438607370659106
50-51	23.251829972167787	26.409152997696783	25.711100971514476	24.62791605862095
52-53	22.92026268873327	26.31439650174204	25.056423824589714	25.70891698493498
54-55	23.557654781134683	26.229754342317747	25.05484200329178	25.15774887325579
56-57	24.300267925738375	25.118262581871104	24.929820812127993	25.65164868026252
58-59	23.530603749785183	25.197885553221994	26.235679760762608	25.035830936230212
60-61	23.857513655606294	25.77703105773817	25.56003080404676	24.805424482608768
62-63	22.918558903909172	25.749438785019013	25.48353523134797	25.848467079723846
64-65	22.675418777366136	25.77234951190403	25.854770477453975	25.69746123327586
66-67	24.197762585102176	25.421886591014804	25.40345274567421	24.976898078208816
68-69	24.247349634934736	24.874387587057022	25.339155856991468	25.539106921016774
70-71	23.161611814726182	25.40159230838215	25.655692588057676	25.78110328883399
72-73	24.32646493509347	25.263725490947525	25.548901685644203	24.8609078883148
74-75	24.66890022758	25.060914523459882	25.52628939005767	24.743895858902444
76-77	23.720240248408793	25.488408309367923	26.108033603709522	24.68331783851376
78-79	23.978192375967158	25.61516085835179	25.779917991042854	24.626728774638202
80-81	23.284001901845784	25.517469908093638	26.234000441311338	24.96452774874924
82-83	23.50017920644058	25.549715809712698	25.377365074710795	25.572739909135926
84-85	23.746014580480114	25.845528090389063	24.75946534208413	25.64899198704669
86-87	23.686693379700728	25.03661345862705	25.356749140490752	25.91994402118147
88-89	23.796935249317062	24.477929919963888	25.75478587838514	25.970348952333904
90-91	24.18134825235812	24.886652019325293	25.35559121696071	25.576408511355876
92-93	23.584222537443665	25.15254905651459	25.38527020633567	25.87795819970608
94-95	22.646924646987756	25.466248953662824	26.055791845960428	25.831034553388992
96-97	23.833186635331536	25.15441403705051	25.22546700023162	25.78693232738633
98-99	23.573375571132686	25.22415196068662	25.13022583322815	26.072246634952545
100	23.216018174951284	25.643890681129673	25.049790828266232	26.090300315652815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1287.0
1	1147.0
2	1276.0
3	1515.0
4	1287.0
5	1165.5
6	1489.0
7	2678.0
8	4363.5
9	4447.0
10	2985.5
11	2041.0
12	1963.5
13	2176.5
14	2497.5
15	3118.0
16	3876.0
17	4773.0
18	5971.0
19	7348.5
20	9111.0
21	11412.5
22	14432.5
23	18261.0
24	22635.0
25	27861.0
26	35503.0
27	44285.5
28	53760.0
29	68409.5
30	85084.5
31	99684.5
32	117889.0
33	136110.5
34	152230.5
35	166311.5
36	180335.0
37	203280.5
38	215021.0
39	216520.0
40	216275.0
41	215728.5
42	225483.5
43	253623.5
44	298922.5
45	350238.0
46	412121.5
47	472874.0
48	540333.5
49	607227.5
50	723509.5
51	723436.0
52	576099.0
53	509560.5
54	482741.0
55	452066.5
56	426604.5
57	387041.0
58	353579.5
59	327084.0
60	280291.5
61	219585.0
62	169632.5
63	131026.5
64	95821.5
65	74400.0
66	57392.5
67	40106.5
68	29792.0
69	24051.0
70	21143.5
71	19961.0
72	18372.0
73	20061.0
74	16322.5
75	11299.5
76	8182.5
77	5763.0
78	4224.0
79	2340.0
80	1373.5
81	1034.0
82	852.5
83	697.0
84	573.5
85	439.5
86	315.0
87	189.0
88	105.5
89	82.5
90	72.5
91	51.5
92	37.0
93	29.5
94	21.5
95	12.5
96	7.0
97	2.5
98	2.5
99	2.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017385938198694197
2	0.006215345193460707
3	0.009782783051077196
4	0.0091867910462248
5	0.019344197643209216
6	0.00406977397599208
7	0.02307340475928564
8	0.007798981092068504
9	0.0013792957826584036
10-11	3.448239456646009E-4
12-13	8.173604637975726E-4
14-15	8.727025785338665E-4
16-17	0.001153670237964282
18-19	0.003256670597943453
20-21	0.006121689306983902
22-23	0.01311608119250167
24-25	0.02240078521095222
26-27	0.026662128045646858
28-29	0.03214099740453996
30-31	0.03489107479835888
32-33	0.0354615242887176
34-35	0.044414175618750384
36-37	0.04139590182274789
38-39	0.0458317851731493
40-41	0.04246868743148221
42-43	0.04953544977473205
44-45	0.042694312976176325
46-47	0.04515916562481588
48-49	0.04771341707418329
50-51	0.04827109530729518
52-53	0.03279233152412865
54-55	0.029284492866997403
56-57	0.02132374251646896
58-59	0.02109811697177484
60-61	0.021379084631205254
62-63	0.021459969260435224
64-65	0.025253032662745836
66-67	0.026696184731638425
68-69	0.027679571539644877
70-71	0.02812230845753523
72-73	0.026266219070994908
74-75	0.020774578454854968
76-77	0.025776654209866152
78-79	0.025474401121691012
80-81	0.02423984625449676
82-83	0.026219391127756505
84-85	0.027764713254623794
86-87	0.03014016710253549
88-89	0.030216794646016506
90-91	0.02994008407233504
92-93	0.03251562095044718
94-95	0.030391335161723283
96-97	0.033056270840563284
98-99	0.033903430904603474
100	0.042655999204435815
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1745124E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.98902018869723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.09561466791257	20.609922089359564
2	14.105244547926949	8.177944379327316
3	5.732432005536192	4.985327614164684
4	2.8793691431873025	3.338803608902742
5	1.593278294269532	2.3093788319396276
6	0.9728333112419857	1.6920890699898656
7	0.6441769510749616	1.3071841047863844
8	0.4447495741171403	1.031428350639702
9	0.33603148620813766	0.8767101183953072
>10	1.7816914640570898	9.608107897512298
>50	0.18013562050442103	3.6362300553206923
>100	0.16849714344401953	10.559105193447275
>500	0.033175388011524566	6.814962081591114
>1k	0.029892548498783426	16.322670770528923
>5k	0.002258960674224785	4.251917812758968
>10k+	6.188933354040507E-4	4.478218021335741
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	87096	0.7415502807803477	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	86773	0.7388002033865287	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	34770	0.2960377429816833	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	25109	0.21378233214055467	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	25061	0.21337365190865587	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	24970	0.21259886230234776	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	23977	0.2041442900049416	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23332	0.1986526493888017	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	20870	0.1776907591609931	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	17346	0.14768681880242387	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	17290	0.14721002519854196	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	15845	0.13490704738408893	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14321	0.12193145002130246	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	14218	0.12105449035701964	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	14190	0.12081609355507868	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.702834299578276E-5	0.0
6	0.0	0.0	0.0	1.702834299578276E-5	8.51417149789138E-6
7	0.0	0.0	0.0	3.405668599156552E-5	8.51417149789138E-6
8	8.51417149789138E-6	0.0	0.0	3.405668599156552E-5	8.51417149789138E-6
9	8.51417149789138E-6	0.0	0.0	3.405668599156552E-5	8.51417149789138E-6
10-11	1.277125724683707E-5	0.0	0.0	3.405668599156552E-5	8.51417149789138E-6
12-13	2.128542874472845E-5	0.0	0.0	5.1085028987348285E-5	8.51417149789138E-6
14-15	3.8313771740511215E-5	0.0	0.0	6.385628623418535E-5	1.277125724683707E-5
16-17	4.25708574894569E-5	0.0	0.0	1.0642714372364226E-4	1.702834299578276E-5
18-19	5.534211473629397E-5	0.0	0.0	2.0008303020044744E-4	1.702834299578276E-5
20-21	8.51417149789138E-5	0.0	0.0	4.25708574894569E-4	1.702834299578276E-5
22-23	8.51417149789138E-5	0.0	0.0	0.0017964901860550815	1.702834299578276E-5
24-25	9.365588647680518E-5	0.0	0.0	0.0055427256451272885	1.702834299578276E-5
26-27	1.5325508696204486E-4	0.0	0.0	0.010353232541435917	2.128542874472845E-5
28-29	1.702834299578276E-4	0.0	8.51417149789138E-6	0.028335162744982517	2.5542514493674142E-5
30-31	1.9156885870255603E-4	0.0	8.51417149789138E-6	0.0811911394038922	2.9799600242619833E-5
32-33	2.128542874472845E-4	0.0	8.51417149789138E-6	0.16803994576813325	4.25708574894569E-5
34-35	2.128542874472845E-4	0.0	8.51417149789138E-6	0.28089528897268345	5.1085028987348285E-5
36-37	2.128542874472845E-4	0.0	8.51417149789138E-6	0.45023790297999405	5.1085028987348285E-5
38-39	2.171113731962302E-4	0.0	8.51417149789138E-6	0.7230872998871702	5.1085028987348285E-5
40-41	2.213684589451759E-4	0.0	8.51417149789138E-6	1.0822065394967306	5.1085028987348285E-5
42-43	2.4265388768990434E-4	0.0	8.51417149789138E-6	1.3729569819782235	5.1085028987348285E-5
44-45	2.511680591877957E-4	0.0	8.51417149789138E-6	1.689756532157515	5.1085028987348285E-5
46-47	2.6393931643463276E-4	0.0	8.51417149789138E-6	2.043035050119522	5.9599200485239666E-5
48-49	2.6393931643463276E-4	0.0	8.51417149789138E-6	2.4086250600674797	9.365588647680518E-5
50-51	2.6393931643463276E-4	0.0	8.51417149789138E-6	2.816453874816477	9.365588647680518E-5
52-53	2.7245348793252417E-4	0.0	8.51417149789138E-6	3.240655441355919	1.0642714372364226E-4
54-55	2.7245348793252417E-4	0.0	8.51417149789138E-6	3.6318773645982794	1.277125724683707E-4
56-57	2.7245348793252417E-4	0.0	8.51417149789138E-6	4.068590506153873	1.277125724683707E-4
58-59	2.7671057368146984E-4	0.0	8.51417149789138E-6	4.54736365490905	1.277125724683707E-4
60-61	2.8096765943041557E-4	0.0	8.51417149789138E-6	5.0143915040828855	1.277125724683707E-4
62-63	2.8096765943041557E-4	0.0	8.51417149789138E-6	5.48728136033302	1.277125724683707E-4
64-65	2.8522474517936124E-4	8.51417149789138E-6	8.51417149789138E-6	5.987501707091385	1.277125724683707E-4
66-67	2.894818309283069E-4	8.51417149789138E-6	8.51417149789138E-6	6.515282426988426	1.277125724683707E-4
68-69	2.894818309283069E-4	8.51417149789138E-6	8.51417149789138E-6	7.092228230200039	1.277125724683707E-4
70-71	2.979960024261983E-4	8.51417149789138E-6	8.51417149789138E-6	7.782276287589641	1.3622674396626208E-4
72-73	2.979960024261983E-4	8.51417149789138E-6	8.51417149789138E-6	8.409055536578414	1.3622674396626208E-4
74-75	3.1502434542198107E-4	8.51417149789138E-6	8.51417149789138E-6	9.008887432776358	1.3622674396626208E-4
76-77	3.2353851691987247E-4	8.51417149789138E-6	8.51417149789138E-6	9.660366293280514	1.3622674396626208E-4
78-79	3.320526884177638E-4	8.51417149789138E-6	8.51417149789138E-6	10.314088637974361	1.3622674396626208E-4
80-81	3.320526884177638E-4	8.51417149789138E-6	8.51417149789138E-6	11.026941903721067	1.4899800121309916E-4
82-83	3.320526884177638E-4	8.51417149789138E-6	8.51417149789138E-6	11.80913032506085	1.702834299578276E-4
84-85	3.320526884177638E-4	8.51417149789138E-6	8.51417149789138E-6	12.5411915617068	1.702834299578276E-4
86-87	3.320526884177638E-4	8.51417149789138E-6	8.51417149789138E-6	13.283499603750458	1.702834299578276E-4
88	3.320526884177638E-4	8.51417149789138E-6	8.51417149789138E-6	13.865949818835459	1.702834299578276E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATA	3260	0.0	29.108364	1
TACATGA	4025	0.0	26.610271	2
GTACAAG	5475	0.0	25.311737	1
GTACTAG	5765	0.0	23.30509	1
GTACAAA	5145	0.0	23.282993	1
TAGGCAT	6940	0.0	22.402819	5
GTATAAG	5310	0.0	21.851763	1
GTGATCG	3945	0.0	20.597158	8
TAGTACT	5810	0.0	20.455753	4
CTAGGCA	8365	0.0	19.935791	4
CTATTGA	5805	0.0	19.823124	9
GTCCTAC	5005	0.0	19.522854	1
TCTAACG	2650	0.0	19.322395	2
CGTTAAC	1675	0.0	19.071205	1
CTAGTAC	7150	0.0	18.724806	3
CTGTGCG	6630	0.0	18.631613	9
CATGGGG	33310	0.0	18.573008	4
CTAACGC	2645	0.0	18.47082	3
TGATCGC	4550	0.0	18.374556	9
AGGGCTA	6470	0.0	18.222315	5
>>END_MODULE