FastQCFastQC Report
Fri 27 May 2016
ERR765499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11261480
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA711440.6317464489569755No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT691330.6138891158178144No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG345200.3065316459293095No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG283070.2513612775585447No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG281570.25002930343081015No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC241520.21446559422029784No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC212590.18877625321005764No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT191490.17003981714659175No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC185200.1644544056376249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173900.15442020054202465No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT173040.15365653537545687No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG133870.11887425098654884No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC133450.11850129823078318No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA133110.11819938409516333No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC82550.021.2360741
TCTACAC98900.020.8620283
TACACTC88300.020.2324355
CATGGGG239850.019.9112364
GTACTAG39800.018.5395471
CTACACT130450.018.2326874
GTATAAG39100.018.1502531
TAGTACT42000.017.9066734
CGTTAAC13650.017.559821
GTCCTAC36950.017.1712471
GTGATCG25800.017.1301218
CTAACGC21300.016.7695853
CTATTGA50900.016.7191079
GGGCTAT54700.016.5885626
ATGGGGG134550.016.5622735
TAGGCAT53200.016.5255245
CATGGGT91200.016.4929894
ATCTACA107900.016.2897822
GTACAAA53700.015.9286741
TGATCGC28150.015.8670989
Produced by FastQC (version 0.11.5)