##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765499_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11261480
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.147946007096756	33.0	33.0	33.0	33.0	33.0
2	32.103386055829255	33.0	33.0	33.0	33.0	33.0
3	32.11077913382611	33.0	33.0	33.0	33.0	33.0
4	35.88258168553334	37.0	37.0	37.0	33.0	37.0
5	35.879591758809674	37.0	37.0	37.0	33.0	37.0
6	35.84107897008209	37.0	37.0	37.0	33.0	37.0
7	35.77425320650572	37.0	37.0	37.0	33.0	37.0
8	35.75460356898028	37.0	37.0	37.0	33.0	37.0
9	35.747172662918196	37.0	37.0	37.0	33.0	37.0
10-11	35.716202976873376	37.0	37.0	37.0	33.0	37.0
12-13	35.69469781058973	37.0	37.0	37.0	33.0	37.0
14-15	37.35742877490348	40.0	37.0	40.0	33.0	40.0
16-17	37.38715124477422	40.0	37.0	40.0	33.0	40.0
18-19	37.41620342086475	40.0	37.0	40.0	33.0	40.0
20-21	37.42969480920803	40.0	37.0	40.0	33.0	40.0
22-23	37.404050977313815	40.0	37.0	40.0	33.0	40.0
24-25	37.37694397184029	40.0	37.0	40.0	33.0	40.0
26-27	37.353211833613344	40.0	37.0	40.0	33.0	40.0
28-29	37.31643163243197	40.0	37.0	40.0	33.0	40.0
30-31	37.22955330915652	40.0	37.0	40.0	33.0	40.0
32-33	37.11165779275903	38.5	37.0	40.0	33.0	40.0
34-35	36.99812062890491	37.0	37.0	40.0	33.0	40.0
36-37	36.928668745138296	37.0	37.0	40.0	33.0	40.0
38-39	36.81896775557031	37.0	37.0	40.0	33.0	40.0
40-41	36.66165979072022	37.0	37.0	40.0	33.0	40.0
42-43	36.545890549021976	37.0	37.0	40.0	33.0	40.0
44-45	36.39169416453255	37.0	37.0	40.0	33.0	40.0
46-47	36.16638026262978	37.0	37.0	40.0	33.0	40.0
48-49	35.940922640718625	37.0	37.0	40.0	30.0	40.0
50-51	35.72270132344949	37.0	37.0	40.0	27.0	40.0
52-53	35.5591965709658	37.0	35.0	40.0	27.0	40.0
54-55	35.41843221317269	37.0	33.0	40.0	27.0	40.0
56-57	35.254427970391106	37.0	33.0	40.0	27.0	40.0
58-59	35.09452469835226	37.0	33.0	40.0	27.0	40.0
60-61	34.82102587759336	37.0	33.0	37.0	27.0	40.0
62-63	34.5979429879554	37.0	33.0	37.0	27.0	40.0
64-65	34.3867742072978	37.0	33.0	37.0	27.0	40.0
66-67	34.15979307337935	37.0	33.0	37.0	27.0	40.0
68-69	33.93238304379176	37.0	33.0	37.0	27.0	40.0
70-71	33.703215918334	37.0	33.0	37.0	27.0	38.5
72-73	33.45682996373478	37.0	33.0	37.0	27.0	37.0
74-75	33.27630875337877	37.0	33.0	37.0	27.0	37.0
76-77	33.06786159545637	37.0	33.0	37.0	27.0	37.0
78-79	32.87812609887865	37.0	33.0	37.0	27.0	37.0
80-81	32.70978921065438	33.0	33.0	37.0	27.0	37.0
82-83	32.556801459488454	33.0	33.0	37.0	27.0	37.0
84-85	32.40692511108664	33.0	33.0	37.0	24.5	37.0
86-87	32.267934809634255	33.0	33.0	37.0	22.0	37.0
88-89	32.14778954453588	33.0	33.0	37.0	22.0	37.0
90-91	32.031359421674594	33.0	33.0	37.0	22.0	37.0
92-93	31.870720100732765	33.0	33.0	37.0	22.0	37.0
94-95	31.789913847913418	33.0	33.0	37.0	22.0	37.0
96-97	31.637428295392787	33.0	33.0	37.0	22.0	37.0
98-99	31.515695050739332	33.0	33.0	37.0	22.0	37.0
100	31.426453094975084	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	14.0
7	283.0
8	1570.0
9	2120.0
10	2135.0
11	3669.0
12	8361.0
13	16413.0
14	22362.0
15	23484.0
16	24860.0
17	28905.0
18	35795.0
19	42688.0
20	52410.0
21	66897.0
22	78641.0
23	78621.0
24	77799.0
25	81445.0
26	88946.0
27	104328.0
28	128182.0
29	165360.0
30	218187.0
31	292170.0
32	400630.0
33	562215.0
34	823342.0
35	1331891.0
36	2363673.0
37	3177620.0
38	955974.0
39	490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48552508156	18.891730791555013	12.518428772146168	25.10431535473882
2	15.96746861978563	21.190056655155896	37.119872392249256	25.722602332809213
3	18.44935507983806	25.971394370151017	29.48816947005794	26.09108107995298
4	13.070296138640291	17.133976665345806	36.18713263745107	33.60859455856283
5	13.90533419336481	37.35148493528405	33.4293630767102	15.31381779464093
6	31.871350715889978	36.699379846489514	17.061001817043657	14.368267620576844
7	26.87758018912077	32.32407823651162	22.020834580337784	18.77750699402983
8	26.96279153204965	32.75949926911621	20.365042263377482	19.912666935456656
9	27.319111925120744	14.438809645895745	19.769324534024975	38.47275389495854
10-11	25.28919753759456	25.60783256367804	26.704963862493386	22.398006036234015
12-13	26.454141129287095	23.540162883472625	26.546240690613914	23.459455296626363
14-15	24.572654259878256	24.180599651276108	24.41533507680478	26.831411012040856
16-17	23.64748335281813	26.644269137987454	25.795812329637513	23.9124351795569
18-19	24.29626884398067	25.814155924702366	27.556520452150295	22.33305477916667
20-21	25.23675266234357	25.23059436251385	26.262604645586958	23.27004832955562
22-23	24.23555379576685	25.25980439646642	25.71471017105874	24.789931636707983
24-25	24.079401330030397	25.84192071535488	25.64389824933559	24.43477970527913
26-27	24.502230490649335	25.29972641286053	25.72584463186193	24.47219846462821
28-29	23.880182705168693	25.020276277742937	26.952986163912957	24.146554853175417
30-31	24.83505575031703	25.063198910760853	26.357016800016268	23.744728538905846
32-33	23.527762655574463	25.565198979305677	26.163878361323857	24.743160003796007
34-35	23.893591156384232	25.628079142549854	26.65925915598248	23.819070545083438
36-37	24.508247868126467	25.434465988486473	25.8946111684424	24.162674974944668
38-39	24.852243936704262	24.605911308176427	26.040917200303	24.500927554816315
40-41	24.144680058567307	25.29479592806859	26.411145852497082	24.14937816086702
42-43	25.170446429717124	25.019994734967693	25.996743032723437	23.812815802591746
44-45	24.66063213807836	25.095479262919984	25.962127624658514	24.281760974343143
46-47	23.9617659129079	25.068034735525764	26.65961193624508	24.310587415321255
48-49	24.920494341882858	25.350506093657522	26.042126275447675	23.68687328901195
50-51	23.846013807410248	25.79041828957908	25.606387570133794	24.757180332876874
52-53	23.62799677666294	25.80747071334673	25.102527822065255	25.46200468792507
54-55	24.28896009450658	25.543095312642254	25.064033983288148	25.10391060956302
56-57	24.445696971182528	24.785997325377316	25.00005328641287	25.76825241702728
58-59	24.027728113550385	24.72795116630798	26.113624183922322	25.13069653621931
60-61	24.278577367756835	25.275996413323202	25.43865701919167	25.00676919972829
62-63	23.359272113761712	25.38968007505297	25.49114740814464	25.75990040304068
64-65	23.212987243190707	25.243284132421877	25.698042075588855	25.845686548798565
66-67	24.30175301948631	25.19108962618252	25.154969249498592	25.352188104832578
68-69	24.602152204840646	24.632123244823745	25.133683048748317	25.632041501587288
70-71	23.58480186187076	24.91968249164456	25.57336728434201	25.92214836214267
72-73	24.63687294468375	24.83367532451789	25.4182611170299	25.111190613768457
74-75	24.66676455488161	24.70073090705834	25.45527677049167	25.177227767568382
76-77	23.930054285115727	25.060968294000517	25.901656261286828	25.107321159596925
78-79	24.21225039923557	25.144961129171882	25.718003533990156	24.92478493760239
80-81	23.62920648655715	24.976173780964317	25.940647227739937	25.453972504738605
82-83	23.775028923179494	25.133300592875784	25.41583910875626	25.675831375188462
84-85	23.987785840053633	25.115005616517223	24.99833267276685	25.898875870662298
86-87	23.731666174117887	24.600693605769642	25.421049285188584	26.246590934923887
88-89	23.979560009450015	24.12800572719687	25.831455192050374	26.060979071302743
90-91	24.23767133411385	24.437362907956153	25.528895627364584	25.79607013056542
92-93	23.72123685619737	24.66630262199629	25.569415488083514	26.043045033722823
94-95	22.935739570876667	24.825022978395182	26.06881561485683	26.17042183587132
96-97	23.830885529800167	24.727944582510034	25.41042909181129	26.030740795878504
98-99	23.58883138652335	24.57432679518472	25.430916972989753	26.40592484530218
100	23.387588635603148	24.84892607145978	25.41289573874192	26.350589554195153
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1664.0
1	1452.5
2	1283.0
3	1179.5
4	830.5
5	711.5
6	928.0
7	1827.0
8	2710.0
9	2453.5
10	1613.0
11	1204.5
12	1337.0
13	1553.0
14	1896.0
15	2411.0
16	3026.5
17	3702.5
18	4478.0
19	5412.0
20	6651.0
21	8178.5
22	10058.5
23	12535.5
24	15522.0
25	19644.0
26	27232.5
27	35997.5
28	43410.5
29	56245.0
30	71446.0
31	84799.5
32	104468.5
33	127145.5
34	151066.5
35	167625.0
36	182585.0
37	206347.0
38	215666.0
39	217277.5
40	220236.5
41	222518.0
42	230216.5
43	257420.5
44	295054.5
45	325617.0
46	363709.5
47	407242.0
48	469984.0
49	541175.0
50	633255.5
51	630706.0
52	541821.0
53	506124.5
54	466669.5
55	423693.5
56	395068.0
57	356638.0
58	335446.0
59	318387.0
60	273686.5
61	225890.0
62	190247.0
63	151970.0
64	116341.0
65	97600.0
66	77662.5
67	57524.0
68	46974.0
69	41060.5
70	37205.0
71	33761.0
72	30652.0
73	32286.0
74	26388.0
75	19869.0
76	15477.5
77	11046.5
78	8411.0
79	5484.0
80	3857.0
81	3043.5
82	2502.5
83	2038.5
84	1555.5
85	1183.0
86	857.0
87	485.5
88	230.0
89	143.0
90	100.5
91	77.5
92	53.0
93	29.0
94	23.5
95	14.5
96	9.0
97	6.5
98	3.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02300763309973467
2	0.006411235468162266
3	0.011090904570269627
4	0.010176282335891908
5	0.012671513868514617
6	0.007823128043560883
7	0.010318359576183591
8	0.010123003370782526
9	0.007974085111370797
10-11	0.029316750551437287
12-13	0.031563346913549555
14-15	0.01393244937610332
16-17	0.0050171025478001115
18-19	0.013777052394534287
20-21	0.002264356017148723
22-23	2.3975534299221771E-4
24-25	0.0034098537670004293
26-27	0.0014962509368218033
28-29	0.0014873711093035728
30-31	0.02645300617680802
32-33	0.020259326482842396
34-35	0.0074324156327587486
36-37	0.008022924162721063
38-39	0.006127080987578898
40-41	0.014336481528182796
42-43	0.003467572645868927
44-45	0.0035164116972191935
46-47	0.012635994558441697
48-49	0.016907191594710463
50-51	0.007636651665678046
52-53	0.0207876762201771
54-55	0.026683881692282013
56-57	0.013976848513694469
58-59	0.010118563457023411
60-61	0.008142801834217171
62-63	0.01508238703971414
64-65	0.008737750277938602
66-67	0.016938270991024268
68-69	0.005412254872361359
70-71	0.004866145479990197
72-73	0.010651353108117228
74-75	0.0029791821323662607
76-77	0.00993652699289969
78-79	0.002410873171199523
80-81	0.006890746154146701
82-83	0.0016294483495952574
84-85	0.00837367734969116
86-87	0.0029125834259795336
88-89	0.0011055385260196706
90-91	0.0010078604233191375
92-93	3.6851284200655686E-4
94-95	0.0031834181652855573
96-97	0.0011144183535379008
98-99	0.004386634794005762
100	0.006819707534000859
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.126148E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.98273810860117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.07031594573127	28.13288572459527
2	13.278007875118316	9.82114175698889
3	4.482594852603435	4.973358944423895
4	1.9431264806037896	2.874485509762313
5	1.072494142746517	1.983188500210158
6	0.6318246209576135	1.4019962692464971
7	0.41037420871461877	1.0623733321192026
8	0.30813790591354556	0.9116626780586754
9	0.22819799045101533	0.7595447865983074
>10	1.2843581775944835	8.909430861418976
>50	0.12185588083928867	3.14844863998818
>100	0.12358045017090444	9.891555990772043
>500	0.02425576269800415	6.32246448045125
>1k	0.019188293292450302	13.13752125063446
>5k	0.001349930051985721	3.201303291482253
>10k+	3.374825129964302E-4	3.468637983250296
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	71144	0.6317464489569755	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	69133	0.6138891158178144	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	34520	0.3065316459293095	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	28307	0.2513612775585447	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	28157	0.25002930343081015	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	24152	0.21446559422029784	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	21259	0.18877625321005764	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	19149	0.17003981714659175	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	18520	0.1644544056376249	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17390	0.15442020054202465	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	17304	0.15365653537545687	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	13387	0.11887425098654884	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	13345	0.11850129823078318	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	13311	0.11819938409516333	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6175103094797486E-4	0.0	0.0	0.0	0.0
2	8.968625793412589E-4	0.0	0.0	8.879827518230286E-6	0.0
3	9.3238188941418E-4	0.0	0.0	8.879827518230286E-6	0.0
4	0.001021180164596483	0.0	0.0	8.879827518230286E-6	0.0
5	0.003516411697219193	0.0	0.0	1.1543775773699371E-4	0.0
6	0.008329278212100008	0.0	0.0	2.3087551547398743E-4	0.0
7	0.009758930442535084	0.0	0.0	2.575149980286783E-4	0.0
8	0.011836810081800972	0.0	0.0	2.8415448058336917E-4	0.0
9	0.013195423692090204	0.0	0.0	3.196737906562903E-4	8.879827518230286E-6
10-11	0.02580477876797721	0.0	0.0	6.2158792627612E-4	8.879827518230286E-6
12-13	0.049078806693258786	4.439913759115143E-6	0.0	0.0012298561112748948	8.879827518230286E-6
14-15	0.07746317535528191	8.879827518230286E-6	0.0	0.0019180427439377417	1.7759655036460573E-5
16-17	0.0900414510348551	8.879827518230286E-6	0.0	0.0023620341198492562	2.6639482554690858E-5
18-19	0.09667468219097311	8.879827518230286E-6	0.0	0.0025307508426956316	3.995922383203629E-5
20-21	0.12113860700369757	8.879827518230286E-6	0.0	0.003387654198204854	4.439913759115143E-5
22-23	0.12769191971215152	8.879827518230286E-6	0.0	0.004320036087619035	6.659870638672715E-5
24-25	0.1574482217257412	8.879827518230286E-6	0.0	0.007467934942831671	7.991844766407258E-5
26-27	0.23412553234565972	8.879827518230286E-6	0.0	0.01793281167306606	7.991844766407258E-5
28-29	0.24947431421092076	8.879827518230286E-6	0.0	0.03619861687806576	7.991844766407258E-5
30-31	0.2556324745948135	8.879827518230286E-6	0.0	0.08770161648380143	7.991844766407258E-5
32-33	0.2608760127443285	8.879827518230286E-6	0.0	0.18251153489594618	9.3238188941418E-5
34-35	0.26437466478651117	8.879827518230286E-6	0.0	0.3097683430597044	9.767810270053315E-5
36-37	0.2714740868873363	1.331974127734543E-5	0.0	0.5075798207695614	1.0655793021876343E-4
38-39	0.2783515133002057	1.7759655036460573E-5	0.0	0.8186179791643726	1.1543775773699373E-4
40-41	0.28446083463274807	1.7759655036460573E-5	0.0	1.2449162987458133	1.24317585255224E-4
42-43	0.3009506743341017	1.7759655036460573E-5	0.0	1.5554305473170489	1.331974127734543E-4
44-45	0.3203086983238438	1.7759655036460573E-5	0.0	1.8808717859464297	1.6427680908726028E-4
46-47	0.3266133758617873	1.7759655036460573E-5	0.0	2.260466652695738	1.731566366054906E-4
48-49	0.33116872737863945	1.7759655036460573E-5	0.0	2.631576844251377	1.86476377882836E-4
50-51	0.341140773681612	1.7759655036460573E-5	0.0	3.040310865001758	1.86476377882836E-4
52-53	0.34390683995354077	1.7759655036460573E-5	0.0	3.4804439558566016	1.9091629164195116E-4
54-55	0.3526445902314793	3.5519310072921146E-5	0.0	3.8747260573210625	1.953562054010663E-4
56-57	0.37270856050892065	3.5519310072921146E-5	0.0	4.294484383935327	1.9979611916018142E-4
58-59	0.3787512831350764	3.5519310072921146E-5	0.0	4.751049595612654	2.0423603291929658E-4
60-61	0.3829070424136082	3.5519310072921146E-5	0.0	5.185139963841342	2.0423603291929658E-4
62-63	0.38636573523195883	3.5519310072921146E-5	0.0	5.628372114500047	2.0423603291929658E-4
64-65	0.3887277693518081	3.5519310072921146E-5	0.0	6.085199281089164	2.0423603291929658E-4
66-67	0.3919911059647577	3.5519310072921146E-5	0.0	6.557046675925367	2.1311586043752686E-4
68-69	0.3952633224052256	3.5519310072921146E-5	0.0	7.094400558363555	2.1311586043752686E-4
70-71	0.3992725645297066	4.439913759115143E-5	0.0	7.7339257362264995	2.1311586043752686E-4
72-73	0.41170432305522897	5.3278965109381715E-5	0.0	8.29439380969464	2.1311586043752686E-4
74-75	0.4239496052028685	5.3278965109381715E-5	0.0	8.82817356155674	2.1311586043752686E-4
76-77	0.42768357267428436	6.2158792627612E-5	0.0	9.41007309874013	2.1311586043752686E-4
78-79	0.43030312179216235	6.2158792627612E-5	0.0	10.00025307508427	2.1311586043752686E-4
80-81	0.4354534217527359	6.2158792627612E-5	0.0	10.651384187513543	2.17555774196642E-4
82-83	0.43716722846375433	6.2158792627612E-5	0.0	11.380475745639117	2.3087551547398743E-4
84-85	0.4438492986712226	6.2158792627612E-5	0.0	12.057669151834395	2.3087551547398743E-4
86-87	0.4562188984041174	6.2158792627612E-5	0.0	12.74340939201597	2.3531542923310256E-4
88	0.4587940483844042	6.2158792627612E-5	0.0	13.28089203195317	2.48635170510448E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	8255	0.0	21.236074	1
TCTACAC	9890	0.0	20.862028	3
TACACTC	8830	0.0	20.232435	5
CATGGGG	23985	0.0	19.911236	4
GTACTAG	3980	0.0	18.539547	1
CTACACT	13045	0.0	18.232687	4
GTATAAG	3910	0.0	18.150253	1
TAGTACT	4200	0.0	17.906673	4
CGTTAAC	1365	0.0	17.55982	1
GTCCTAC	3695	0.0	17.171247	1
GTGATCG	2580	0.0	17.130121	8
CTAACGC	2130	0.0	16.769585	3
CTATTGA	5090	0.0	16.719107	9
GGGCTAT	5470	0.0	16.588562	6
ATGGGGG	13455	0.0	16.562273	5
TAGGCAT	5320	0.0	16.525524	5
CATGGGT	9120	0.0	16.492989	4
ATCTACA	10790	0.0	16.289782	2
GTACAAA	5370	0.0	15.928674	1
TGATCGC	2815	0.0	15.867098	9
>>END_MODULE