FastQCFastQC Report
Fri 27 May 2016
ERR765498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10686630
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT166710.15599866375087376No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA162310.15188136952434958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131160.12273279789793415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA411800.019.1538091
GTACATG616950.018.811381
TACATGG616700.018.3287052
CATGGGG228200.017.628784
ACATGGG600350.017.183213
GTACTAG35150.016.8498021
GTCCTAC35550.016.7924351
GTATTAG38400.016.6478161
GTATAAG49800.016.518031
ATACGGG25300.016.3469413
AACGCAG463750.015.9380726
ATGGGTA47800.015.925775
CATGGGT77100.015.787364
GTCCTAT52200.015.758581
TAGGCAT49250.015.64771655
CGTTAAC10900.015.5247831
CAACGCA465000.015.4514025
ATCAACG466450.015.4458343
TATCAAC516950.015.2921332
TAGTACT42300.015.2210424
Produced by FastQC (version 0.11.5)