FastQCFastQC Report
Fri 27 May 2016
ERR765494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16281896
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA523710.32165172901239514No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT518990.31875280372752657No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307750.1890136136479437No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA250240.153692174424895No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA234270.14388373442503255No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA202860.12459236934076964No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG200230.12297707834517553No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC193380.11876995160760148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179950.11052152648561321No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC176310.10828591461338409No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG167800.10305925059341983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA21300.023.3885048
TAGTACT92300.023.3206124
GTACTAG78200.022.7782651
TATTCGC12000.022.7155329
CTATTGA89600.022.607139
GTATAAG75750.022.3361361
ATGGGAG185250.022.325475
GGGCTAT102300.021.73016
GTGATCG61650.021.6501778
CGTTAAC27300.021.5196671
CTGTGCG100700.021.3752989
GTATCAA1042250.021.180531
GTCCTAC69200.020.7148571
AGGGCTA102000.020.6881835
TAGGCAT115700.020.6757185
CATGGGG481150.020.6588444
CTAGTAC114850.020.296583
TTTAGGG109200.020.1850762
GGCTATT109800.020.1601967
CTAGGCA129050.019.3380494
Produced by FastQC (version 0.11.5)