FastQCFastQC Report
Fri 27 May 2016
ERR765492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7381578
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA106370.1441019792786854No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT98770.13380607777903317No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA228050.024.2032281
GTACTAG26250.023.5599821
GTATAAG32450.021.6378881
TAGTACT34350.020.4407564
TATCAAC274350.019.9151062
CAACGCA246250.019.770485
ATCAACG250600.019.4088733
GTACATG354900.019.1424221
AACGCAG264150.018.888436
TACATGG355750.018.1293092
TCAACGC269800.018.0792454
CTAGTAC35500.017.9449373
ATACGGG19600.017.555993
ACGCAGA279450.017.1720587
CGCAGAG277400.017.0810478
ATAAGGT27800.017.0610243
TACGGGC19900.017.0575454
TCTAACG12100.016.5246682
TGATCGC23800.016.411539
CGGGCGT20450.016.3714036
Produced by FastQC (version 0.11.5)