##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765492_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7381578 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.501179964500814 33.0 33.0 33.0 27.0 33.0 2 31.391619109084804 33.0 33.0 33.0 27.0 33.0 3 31.439586494920192 33.0 33.0 33.0 27.0 33.0 4 35.02268918651269 37.0 37.0 37.0 33.0 37.0 5 35.184519759867065 37.0 37.0 37.0 33.0 37.0 6 35.18656674765206 37.0 37.0 37.0 33.0 37.0 7 35.07224566346112 37.0 37.0 37.0 33.0 37.0 8 35.004349205549275 37.0 37.0 37.0 33.0 37.0 9 34.93741582084481 37.0 37.0 37.0 33.0 37.0 10-11 34.90873435192312 37.0 37.0 37.0 33.0 37.0 12-13 34.870460286404885 37.0 37.0 37.0 33.0 37.0 14-15 36.38996052063665 37.0 37.0 40.0 33.0 40.0 16-17 36.44905784372935 37.0 37.0 40.0 33.0 40.0 18-19 36.471165921433055 38.5 37.0 40.0 33.0 40.0 20-21 36.49345180664622 37.0 37.0 40.0 33.0 40.0 22-23 36.54036074671296 37.0 37.0 40.0 33.0 40.0 24-25 36.51785200942129 37.0 37.0 40.0 33.0 40.0 26-27 36.53385773340064 37.0 37.0 40.0 33.0 40.0 28-29 36.48063205455527 37.0 37.0 40.0 33.0 40.0 30-31 36.460311738221826 37.0 37.0 40.0 33.0 40.0 32-33 36.35492051970459 37.0 37.0 40.0 33.0 40.0 34-35 36.312298942041934 37.0 37.0 40.0 33.0 40.0 36-37 36.216335653433454 37.0 37.0 40.0 27.0 40.0 38-39 36.032318225181655 37.0 37.0 40.0 27.0 40.0 40-41 35.96273940341753 37.0 37.0 40.0 27.0 40.0 42-43 35.84951537462586 37.0 37.0 40.0 27.0 40.0 44-45 35.72215588590949 37.0 37.0 40.0 27.0 40.0 46-47 35.619987758715006 37.0 35.0 40.0 27.0 40.0 48-49 35.470295037185814 37.0 33.0 40.0 27.0 40.0 50-51 35.32178295751938 37.0 33.0 40.0 27.0 40.0 52-53 35.14876609039422 37.0 33.0 40.0 27.0 40.0 54-55 35.03962005143073 37.0 33.0 40.0 27.0 40.0 56-57 34.85993916205993 37.0 33.0 40.0 27.0 40.0 58-59 34.63049851942227 37.0 33.0 40.0 27.0 40.0 60-61 34.40750013073085 37.0 33.0 37.0 27.0 40.0 62-63 34.228110981147935 37.0 33.0 37.0 27.0 40.0 64-65 33.91565793926448 37.0 33.0 37.0 27.0 40.0 66-67 33.696093572404166 37.0 33.0 37.0 27.0 40.0 68-69 33.39905938811457 37.0 33.0 37.0 27.0 40.0 70-71 33.255152963228184 37.0 33.0 37.0 27.0 38.5 72-73 32.98693917479433 37.0 33.0 37.0 24.5 37.0 74-75 32.711658536968656 37.0 33.0 37.0 22.0 37.0 76-77 32.51919088303341 33.0 33.0 37.0 22.0 37.0 78-79 32.276136891055 33.0 33.0 37.0 22.0 37.0 80-81 32.0245165735565 33.0 33.0 37.0 22.0 37.0 82-83 31.84306472139155 33.0 33.0 37.0 22.0 37.0 84-85 31.671784542546323 33.0 33.0 37.0 22.0 37.0 86-87 31.477458546126588 33.0 33.0 37.0 22.0 37.0 88-89 31.31834046866402 33.0 33.0 37.0 22.0 37.0 90-91 31.12014599046437 33.0 27.0 37.0 22.0 37.0 92-93 30.97977349829535 33.0 27.0 37.0 18.5 37.0 94-95 30.75895296371589 33.0 27.0 37.0 15.0 37.0 96-97 30.670261426486313 33.0 27.0 37.0 15.0 37.0 98-99 29.537630571674512 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27708254421862577 1101 2 0.33824298708505296 1101 3 0.3360284544331549 1101 4 0.39494843083405584 1101 5 0.373667283614985 1101 6 0.3463088029746828 1101 7 0.3288914044350335 1101 8 0.3940541546503411 1101 9 0.301859275421954 1101 10-11 0.3995525468685912 1101 12-13 0.3483360871562766 1101 14-15 0.46607035028376487 1101 16-17 0.4036210341685589 1101 18-19 0.38180546910559343 1101 20-21 0.4289611241909199 1101 22-23 0.33279982751923853 1101 24-25 0.41247581588110194 1101 26-27 0.43012127682501244 1101 28-29 0.36426947337378834 1101 30-31 0.4019148677071982 1101 32-33 0.43964399587512304 1101 34-35 0.4277662309102297 1101 36-37 0.42385909692443136 1101 38-39 0.3880918583042998 1101 40-41 0.4093547101704331 1101 42-43 0.40369011936331134 1101 44-45 0.4739214357599124 1101 46-47 0.4548953433685412 1101 48-49 0.41023005848090577 1101 50-51 0.416525546904154 1101 52-53 0.29455869087937003 1101 54-55 0.4824068746547212 1101 56-57 0.5094177360553331 1101 58-59 0.42189666277149485 1101 60-61 0.4668558205076323 1101 62-63 0.4675214156741063 1101 64-65 0.3683809288834752 1101 66-67 0.3825493935162143 1101 68-69 0.41237983942213674 1101 70-71 0.39867052105092426 1101 72-73 0.41139275008907816 1101 74-75 0.3835183218537921 1101 76-77 0.34885221899578767 1101 78-79 0.36127710350493913 1101 80-81 0.3711434822272892 1101 82-83 0.426612233644029 1101 84-85 0.35581679061920113 1101 86-87 0.41094187373459334 1101 88-89 0.41357805860805286 1101 90-91 0.42959880237670944 1101 92-93 0.39419821303996727 1101 94-95 0.44485835490143444 1101 96-97 0.3702149325272792 1101 98-99 0.3436280793712001 1102 1 0.11312204942698756 1102 2 0.18641053146911446 1102 3 0.2060635431437845 1102 4 0.23255687111323198 1102 5 0.1398706535865628 1102 6 0.19792745588127048 1102 7 0.1481387896595976 1102 8 0.14432312867575803 1102 9 0.08990421998412756 1102 10-11 0.08048477743812299 1102 12-13 0.16097347646356042 1102 14-15 0.1626932661857623 1102 16-17 0.19966836150738487 1102 18-19 0.2463106097088783 1102 20-21 0.20662386602177207 1102 22-23 0.11603723353191242 1102 24-25 0.255955297284693 1102 26-27 0.2659495418027191 1102 28-29 0.16560462743584736 1102 30-31 0.16867507990343 1102 32-33 0.26775551231961714 1102 34-35 0.16389665072719595 1102 36-37 0.29864843366214444 1102 38-39 0.29103348528608564 1102 40-41 0.22604294299725325 1102 42-43 0.22184084994487563 1102 44-45 0.12210294236761854 1102 46-47 0.2026689559321042 1102 48-49 0.13179520978911086 1102 50-51 0.13565506632194513 1102 52-53 0.08304311285685628 1102 54-55 0.1907373655804676 1102 56-57 0.1581741413280966 1102 58-59 0.1328496247792117 1102 60-61 0.17636586061945536 1102 62-63 0.18822934394211188 1102 64-65 0.13450711650405367 1102 66-67 0.1424642683025823 1102 68-69 0.12311377520625655 1102 70-71 0.11365749799214342 1102 72-73 0.1275055627207209 1102 74-75 0.06179151674154326 1102 76-77 0.09794253906004258 1102 78-79 0.0874076966177384 1102 80-81 0.06812725179934631 1102 82-83 0.12430029062895187 1102 84-85 0.03469950638011454 1102 86-87 0.1495648215033114 1102 88-89 0.13434118693788832 1102 90-91 0.11542404905018344 1102 92-93 0.14382177095785664 1102 94-95 0.11713151825099288 1102 96-97 0.12635429473783333 1102 98-99 0.08169976159164705 1103 1 0.03468113562736974 1103 2 0.12685395766295215 1103 3 0.17034526559293894 1103 4 0.1685522759043181 1103 5 0.14295430781929497 1103 6 0.1641777022637001 1103 7 0.015455224960277292 1103 8 0.08082896599125178 1103 9 0.16508051514014 1103 10-11 0.09507855872546145 1103 12-13 0.07257380310549166 1103 14-15 0.056452121721314086 1103 16-17 0.014021122036261602 1103 18-19 0.09096385465326762 1103 20-21 0.10924021525312355 1103 22-23 0.1239426756503974 1103 24-25 0.16312322726775363 1103 26-27 0.19236319958917392 1103 28-29 0.1257027257954988 1103 30-31 0.15844266930745476 1103 32-33 0.18989046924821906 1103 34-35 0.13861474522225592 1103 36-37 0.18114047210821838 1103 38-39 0.2383259028926048 1103 40-41 0.12124159243799681 1103 42-43 0.19616168028010605 1103 44-45 0.08953967608395175 1103 46-47 0.07812333922992565 1103 48-49 0.11235158875027906 1103 50-51 0.11522031569649727 1103 52-53 0.06876990125010707 1103 54-55 0.16580713389740964 1103 56-57 0.027911903217180623 1103 58-59 0.14139075576680682 1103 60-61 0.17232380801421243 1103 62-63 0.09251475899870343 1103 64-65 0.1306295032252649 1103 66-67 0.169720103821426 1103 68-69 0.11638576856747562 1103 70-71 0.12500837781864504 1103 72-73 0.16511000525125752 1103 74-75 0.20614390663696014 1103 76-77 0.14808765134543478 1103 78-79 0.12464616485021196 1103 80-81 0.18146572420649676 1103 82-83 0.07085503056659803 1103 84-85 0.1243224114633108 1103 86-87 0.2096959700318024 1103 88-89 0.13573880210940814 1103 90-91 0.16494899627529236 1103 92-93 0.18649416578213263 1103 94-95 0.18015529539676578 1103 96-97 0.03444728626906013 1103 98-99 0.10847770604233276 1104 1 0.11349589368296975 1104 2 0.024854066032368394 1104 3 -0.016810406505673114 1104 4 0.045730210520261494 1104 5 0.08882551179517861 1104 6 0.03638623696087251 1104 7 -0.03144190920919243 1104 8 0.011554661038381653 1104 9 0.02617114172872448 1104 10-11 0.05737834887487736 1104 12-13 0.019684285626318854 1104 14-15 0.0534444873082478 1104 16-17 0.11383796369027976 1104 18-19 0.02713785546915659 1104 20-21 0.038820666462740405 1104 22-23 0.05146493080677317 1104 24-25 0.04564405472122246 1104 26-27 0.09358104625535901 1104 28-29 0.05937115380260849 1104 30-31 0.08371792895017194 1104 32-33 0.08209631899111258 1104 34-35 0.059320189935064604 1104 36-37 0.1397604131920076 1104 38-39 0.06508748849572754 1104 40-41 0.09919057858098768 1104 42-43 0.010382946697468753 1104 44-45 -0.017377850798887096 1104 46-47 -0.047190141725927504 1104 48-49 0.03723324090419311 1104 50-51 0.03979246886953547 1104 52-53 0.014607978904805918 1104 54-55 0.12203189635369682 1104 56-57 0.003829765493428283 1104 58-59 0.07924929031434402 1104 60-61 0.09029076311932727 1104 62-63 0.06133157707345305 1104 64-65 0.008201876210591763 1104 66-67 0.01465552411272597 1104 68-69 0.008643629014343901 1104 70-71 0.07017692706575218 1104 72-73 0.03477812258969237 1104 74-75 -0.034023749090849265 1104 76-77 -0.013566456668897331 1104 78-79 -0.04141799508632715 1104 80-81 -0.033811431699298566 1104 82-83 0.04910342008812307 1104 84-85 0.0020102889487922937 1104 86-87 0.016423464040247637 1104 88-89 0.11541248255630521 1104 90-91 0.014198227511108996 1104 92-93 0.05757500596889287 1104 94-95 0.11734110708323442 1104 96-97 -0.12064180038320771 1104 98-99 0.09834600074231403 1105 1 -0.04155551335996677 1105 2 0.030879517371285914 1105 3 0.0154494591208163 1105 4 -0.004344323096894698 1105 5 0.0040942536055936785 1105 6 0.021179845445310264 1105 7 -0.045991457970004035 1105 8 -0.050829634525797474 1105 9 -0.06806931876096911 1105 10-11 -0.05008098645519965 1105 12-13 -0.07995724337589394 1105 14-15 -0.03638072618972643 1105 16-17 -0.08244573004778744 1105 18-19 -0.04542692549822647 1105 20-21 -0.02760128108948834 1105 22-23 -0.04283647217320663 1105 24-25 -0.02227906000688762 1105 26-27 0.01498182804713366 1105 28-29 -0.022404399580246093 1105 30-31 -0.01077560192904059 1105 32-33 0.051105292972870586 1105 34-35 -0.06875953684064484 1105 36-37 -0.034588060944116705 1105 38-39 -0.12110710769579214 1105 40-41 -0.06975469702156545 1105 42-43 -0.036413154062891806 1105 44-45 -0.11259014235334064 1105 46-47 -0.05780325232164785 1105 48-49 -0.09652481544259928 1105 50-51 0.018592918726795915 1105 52-53 -0.05098007933877824 1105 54-55 0.011145349182086761 1105 56-57 0.025747630400452692 1105 58-59 -0.05433937561031854 1105 60-61 0.003938455391207185 1105 62-63 0.024910872577635246 1105 64-65 -0.11440626043957991 1105 66-67 0.012866895665290201 1105 68-69 -0.0462246344387367 1105 70-71 -0.06646234307102361 1105 72-73 -0.02910357379538908 1105 74-75 -0.02291308150187632 1105 76-77 -0.07262216633844076 1105 78-79 -0.03961401456190572 1105 80-81 -0.10664799140936765 1105 82-83 -0.0660244568606494 1105 84-85 -0.06583173766933825 1105 86-87 -0.039107845107402284 1105 88-89 -0.015879741975815875 1105 90-91 -0.046106526505582224 1105 92-93 -0.030967895039996307 1105 94-95 -0.04932346937585663 1105 96-97 -0.06148282251525927 1105 98-99 0.029235162385376157 1106 1 0.047860582179701794 1106 2 0.13948920151112176 1106 3 0.07679006357636808 1106 4 0.023433940170264123 1106 5 0.05153598182399577 1106 6 -0.005749797859259331 1106 7 0.04810307628997634 1106 8 -0.01775385040725297 1106 9 -0.03423186176594584 1106 10-11 -0.10499757995997072 1106 12-13 0.026991475212604144 1106 14-15 0.029873034660525377 1106 16-17 0.02195133372791247 1106 18-19 0.03307162412642839 1106 20-21 0.0010188401049120444 1106 22-23 2.963192301876916E-4 1106 24-25 0.01802166101253988 1106 26-27 0.018820087743726788 1106 28-29 0.004269756388730173 1106 30-31 -0.026555685684662933 1106 32-33 0.10156170908253159 1106 34-35 -0.08363549623825861 1106 36-37 -0.11632933920477484 1106 38-39 -0.07132880613107062 1106 40-41 0.022398521879402722 1106 42-43 -0.013584267406095307 1106 44-45 -0.007748304541991047 1106 46-47 -0.06396130019786739 1106 48-49 -0.0846627934586337 1106 50-51 -0.06845619364968059 1106 52-53 -0.03622859090764052 1106 54-55 -0.08704901716820501 1106 56-57 -0.07013910241268917 1106 58-59 0.026338386906466837 1106 60-61 0.0808250990367867 1106 62-63 0.010557914835132465 1106 64-65 -0.1154896870507045 1106 66-67 -0.06509595607997909 1106 68-69 -0.11256226309583184 1106 70-71 -0.14553143362562793 1106 72-73 -0.03596409965968661 1106 74-75 -0.11420740199179846 1106 76-77 -0.11443904941538108 1106 78-79 -0.025562195630492113 1106 80-81 -0.12015716474087412 1106 82-83 -0.027796419597500233 1106 84-85 -0.14898305503800202 1106 86-87 -0.18809374289815395 1106 88-89 0.01038870347751697 1106 90-91 -0.029518286683110517 1106 92-93 -0.06168440193036773 1106 94-95 -0.14681575618750742 1106 96-97 -0.20735744904143516 1106 98-99 0.18372575540001534 1107 1 0.0047095211205885334 1107 2 0.09281875421274677 1107 3 0.032013292694106354 1107 4 -0.04341664086318531 1107 5 0.018243777181076837 1107 6 0.010266371024236776 1107 7 0.008353170805769139 1107 8 -0.011144275098217804 1107 9 -0.09289233430672539 1107 10-11 -0.012285740472613327 1107 12-13 -0.0867298693175087 1107 14-15 -0.03703826560946766 1107 16-17 -0.021505691182248654 1107 18-19 -0.09137061813186875 1107 20-21 -0.07241649390817173 1107 22-23 -0.10247251614430297 1107 24-25 0.046839889003720714 1107 26-27 -0.0059502484269913225 1107 28-29 -0.051626711584738416 1107 30-31 -0.04983557463338428 1107 32-33 -0.1557650406052815 1107 34-35 -0.1359940185077022 1107 36-37 -0.12592628185755927 1107 38-39 -0.11916252325580245 1107 40-41 -0.264279887658482 1107 42-43 -0.26263359128402897 1107 44-45 -0.19852394505356585 1107 46-47 -0.2813605692757335 1107 48-49 -0.24001578607685303 1107 50-51 -0.21608344627607323 1107 52-53 -0.24551405151460415 1107 54-55 -0.17630438547428895 1107 56-57 -0.149083981566136 1107 58-59 -0.11239395582498446 1107 60-61 -0.08683637021599111 1107 62-63 -0.3073487990321553 1107 64-65 -0.34552504932305794 1107 66-67 -0.08659174964984828 1107 68-69 -0.12342552643020355 1107 70-71 -0.1585525020550591 1107 72-73 -0.1497188206758011 1107 74-75 -0.1968729955700681 1107 76-77 -0.28598339928038996 1107 78-79 -0.11985298349122075 1107 80-81 -0.29873289766788247 1107 82-83 -0.1866458473473358 1107 84-85 -0.2427573475061422 1107 86-87 -0.3119996509944336 1107 88-89 -0.22939982937005965 1107 90-91 -0.20063895757339267 1107 92-93 -0.18213313236369189 1107 94-95 -0.110955433344472 1107 96-97 -0.22203021436349246 1107 98-99 0.09625444428457541 1108 1 0.038284609528481894 1108 2 0.0993284801039529 1108 3 0.06839490839809415 1108 4 0.07956486564642518 1108 5 0.11262669939895176 1108 6 -0.0680026981005355 1108 7 0.0545355045014162 1108 8 7.471963163396822E-5 1108 9 0.05834703549353293 1108 10-11 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-0.07969376318579435 2215 78-79 -0.030670760202532676 2215 80-81 0.04399614184206513 2215 82-83 -0.05679185367975492 2215 84-85 -0.02839411601731001 2215 86-87 -0.016659979289574522 2215 88-89 -0.16748938769218213 2215 90-91 0.08008844480045596 2215 92-93 0.11748789700690665 2215 94-95 0.10479238643100786 2215 96-97 0.10605864621870964 2215 98-99 -0.0031182917833874058 2216 1 0.008866413822573804 2216 2 -0.01998200664511174 2216 3 0.04969725242197143 2216 4 0.10376723618884398 2216 5 0.03826436005969214 2216 6 0.030217009750074908 2216 7 0.017591424131715883 2216 8 -5.481763564532116E-4 2216 9 0.049185319317182064 2216 10-11 0.010300005173299098 2216 12-13 0.03422249819397649 2216 14-15 -0.011063361427353868 2216 16-17 -0.009839310778140486 2216 18-19 -0.07686965785109834 2216 20-21 -0.05948841627537149 2216 22-23 -0.16412948791881377 2216 24-25 -0.09571043815634539 2216 26-27 -0.16151730453085378 2216 28-29 -0.026316026624492395 2216 30-31 -0.05769213066657386 2216 32-33 -0.05215296761193855 2216 34-35 -0.12531577442223352 2216 36-37 -0.13547757230114854 2216 38-39 -0.09220757017283887 2216 40-41 -0.11224182835048424 2216 42-43 -0.11382651543406297 2216 44-45 -0.11019024538422428 2216 46-47 0.014988502092528222 2216 48-49 0.053786549425268504 2216 50-51 7.23318035795728E-5 2216 52-53 0.17236534747014076 2216 54-55 0.056294274016202905 2216 56-57 0.12845901086652844 2216 58-59 -0.06945491213411259 2216 60-61 -0.028014899162741358 2216 62-63 0.060557424556122896 2216 64-65 0.13867503899795253 2216 66-67 0.050830273830079875 2216 68-69 0.03600625439917593 2216 70-71 -9.456850825344532E-4 2216 72-73 -0.048604128279933434 2216 74-75 0.07118591806551677 2216 76-77 -0.01495884555971827 2216 78-79 0.06259115427193507 2216 80-81 0.17411087464007124 2216 82-83 0.12420620615613487 2216 84-85 0.16795201512125857 2216 86-87 0.09501457245131206 2216 88-89 0.06439230677721497 2216 90-91 0.23466888678144926 2216 92-93 0.31462027986600916 2216 94-95 0.29050006163552666 2216 96-97 0.26053466414307636 2216 98-99 0.2478244142610393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 205.0 8 1245.0 9 2210.0 10 3227.0 11 5874.0 12 11071.0 13 19706.0 14 28424.0 15 30934.0 16 32882.0 17 35802.0 18 39160.0 19 42322.0 20 46073.0 21 50287.0 22 54875.0 23 60731.0 24 69546.0 25 77437.0 26 85466.0 27 94518.0 28 112595.0 29 138551.0 30 175231.0 31 225069.0 32 301633.0 33 408071.0 34 564402.0 35 825128.0 36 1326433.0 37 1863273.0 38 648621.0 39 574.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11817700512475 18.385352118476675 12.25187077419624 25.244600102202337 2 15.928843399067247 20.390057518866563 38.22196283775637 25.45913624430982 3 18.37009426723428 25.585322793205993 28.799978541144643 27.244604398415078 4 13.080089379262807 17.171192934627257 36.324157246594154 33.42456043951578 5 14.413381528990143 37.406947403387186 33.54888345012408 14.630787617498589 6 32.063442261261756 36.63971308031968 17.45259617929933 13.844248479119234 7 28.128904144886093 31.804012637948148 21.64141596823877 18.42566724892699 8 26.791439445603636 34.14936481061366 19.625966155204214 19.433229588578484 9 26.867060132670815 15.642956018347295 18.918258399491275 38.571725449490614 10-11 25.316016439845246 25.813125594554442 27.098833067942923 21.772024897657385 12-13 26.28188037842315 23.61860837885883 26.422731020386152 23.676780222331864 14-15 24.05523588587698 24.385808833829298 23.85481126122355 27.704144019070178 16-17 24.03371609701882 26.941515757199884 24.55206054857105 24.472707597210245 18-19 24.284150353759046 26.705739612857847 25.601016476422796 23.409093556960315 20-21 25.643760631238866 26.030637476041612 24.747573273248374 23.578028619471148 22-23 25.394204328667936 25.668535914678408 24.592241658897326 24.34501809775633 24-25 24.685391955398327 26.044653966375336 24.657794996184926 24.61215908204141 26-27 24.81261795413094 26.1031958347112 24.510358694354878 24.573827516802982 28-29 24.619148474226307 26.139208114427355 24.81162428434638 24.430019126999962 30-31 24.790053887151146 26.02239208446696 24.811533153596603 24.376020874785294 32-33 24.614846685466183 26.291406567989874 24.495495438743514 24.59825130780043 34-35 24.679602526480295 26.3537509990248 24.785149128434472 24.18149734606043 36-37 24.73404738119681 26.018210469360348 24.73077572302291 24.516966426419934 38-39 24.76704839183394 26.10200721363568 24.726603099392324 24.404341295138057 40-41 24.693439532847854 25.89390100599086 24.97338644934728 24.439273011814006 42-43 24.635782484449802 25.96672418824268 24.862996773860548 24.53449655344697 44-45 24.476162143108155 26.243765222016215 24.831655236861277 24.448417398014357 46-47 24.283398482004795 26.29828608462852 24.943284484699614 24.475030948667072 48-49 24.60617602052031 26.021864874056867 24.9575378014325 24.414421303990324 50-51 24.052472249158647 26.272187329050777 24.951785377056236 24.72355504473434 52-53 24.12633179517984 26.283567009655663 24.684904772394194 24.905196422770306 54-55 24.212620932814094 26.070827944919095 24.718515471895035 24.99803565037178 56-57 24.38366159647707 25.95168675315766 24.756664496399008 24.907987153966264 58-59 24.19985943385005 25.961325613574765 25.091369352190007 24.747445600385177 60-61 24.37392790538825 26.117606560548435 24.756711911734865 24.75175362232845 62-63 24.210040183819775 26.07915949679052 24.68134862220517 25.02945169718453 64-65 23.936284355458955 26.05677945826759 24.903895887844037 25.103040298429413 66-67 24.527709130871003 25.909988845197514 24.7375898419497 24.824712181981784 68-69 24.277139657672112 25.907759831298943 24.843874846272705 24.971225664756236 70-71 24.142845879295727 26.013624729021355 24.70225201169723 25.141277379985688 72-73 24.525995661090352 26.024747012088746 24.753406385463922 24.695850941356984 74-75 24.601623121776942 26.12433276462025 24.69247090527256 24.58157320833025 76-77 24.410261599890973 26.04440405560979 24.808618157255808 24.73671618724343 78-79 24.48763665438474 26.058926695619828 24.78594685309835 24.667489796897087 80-81 24.364072289150098 26.000720984049753 25.019508023894076 24.615698702906073 82-83 24.596624190654083 25.863582285522146 24.971801422405886 24.567992101417882 84-85 24.73890406631211 25.89390100599086 24.669359315853605 24.69783561184343 86-87 24.43937461610512 25.736624336964265 25.028395012556935 24.79560603437368 88-89 24.712554686816286 25.319240682683297 25.0675939480691 24.900610682431317 90-91 24.782234909662947 25.503428941616548 24.90429553138909 24.810040617331417 92-93 24.790796764594237 25.423574742419575 24.930942950138846 24.854685542847342 94-95 24.358213108362467 25.458655317331875 25.245482740953225 24.937648833352437 96-97 24.638742556130953 25.42233517006797 24.99323315421174 24.945689119589336 98-99 24.639108331578967 25.68195445472499 24.64878783371252 25.03014937998352 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1107.0 1 1046.5 2 1012.5 3 1035.5 4 938.0 5 838.0 6 767.0 7 697.5 8 691.0 9 652.0 10 597.0 11 586.5 12 599.5 13 617.5 14 633.5 15 799.0 16 1002.5 17 1132.0 18 1430.0 19 1937.5 20 2709.5 21 3779.0 22 5290.5 23 7340.5 24 10063.5 25 14223.0 26 19428.0 27 24869.5 28 32394.0 29 41869.5 30 50028.5 31 59469.0 32 69964.5 33 80746.5 34 92152.5 35 103059.5 36 112546.0 37 124808.0 38 141158.0 39 148456.5 40 155613.0 41 168586.5 42 180962.0 43 198050.5 44 221210.0 45 248501.0 46 273164.5 47 294911.5 48 309801.5 49 324130.0 50 343258.5 51 345251.0 52 337505.0 53 335801.5 54 322584.5 55 307229.0 56 286944.0 57 260870.0 58 242220.0 59 212980.5 60 176972.0 61 144417.0 62 117634.0 63 94108.0 64 70753.0 65 52182.5 66 40235.0 67 31757.0 68 24922.0 69 20425.0 70 16515.5 71 12996.5 72 11004.5 73 9166.5 74 7162.0 75 5427.0 76 4032.5 77 2945.0 78 2169.0 79 1543.0 80 1100.0 81 810.0 82 523.5 83 344.5 84 262.0 85 184.5 86 124.5 87 87.0 88 64.5 89 49.5 90 35.0 91 25.5 92 19.0 93 12.5 94 10.0 95 8.0 96 7.0 97 6.5 98 6.0 99 19.0 100 31.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0019237079117771293 2 0.0 3 1.3547238815331898E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.06417164459957E-4 22-23 0.0 24-25 0.004470588809059526 26-27 1.35472388153319E-5 28-29 0.0088124788493734 30-31 5.554367914286077E-4 32-33 6.096257466899354E-5 34-35 6.096257466899354E-5 36-37 0.0 38-39 2.70944776306638E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.6934048519164871E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.41889552613276E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7381578.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.62179286853827 #Duplication Level Percentage of deduplicated Percentage of total 1 74.73855899690939 35.59184175843846 2 14.105933884221573 13.434997233033902 3 4.705360334914281 6.722330857233712 4 2.1037861440940007 4.007442719749813 5 1.1458404004652978 2.728348710568068 6 0.6708240662070863 1.9167506839286679 7 0.46204290678714166 1.540231812237622 8 0.3223973420475034 1.2282511555482793 9 0.2327323695152034 0.9974819425371415 >10 1.2685370192632552 11.264302543155246 >50 0.12596368184160062 4.217007231394604 >100 0.10373429302209707 9.845799135099766 >500 0.010602282636368268 3.3638996633359715 >1k 0.0036005506786005357 2.7907917629672956 >5k 5.71515977054565E-5 0.20577122133920706 >10k+ 2.857579885272825E-5 0.14475156943225617 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 10637 0.1441019792786854 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9877 0.13380607777903317 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.7094477630663795E-5 0.0 1.3547238815331898E-5 0.0 6 0.0 2.7094477630663795E-5 0.0 1.3547238815331898E-5 0.0 7 0.0 2.7094477630663795E-5 0.0 1.3547238815331898E-5 0.0 8 4.0641716445995696E-5 2.7094477630663795E-5 0.0 1.3547238815331898E-5 0.0 9 4.0641716445995696E-5 2.7094477630663795E-5 0.0 1.3547238815331898E-5 0.0 10-11 7.450981348432545E-5 2.7094477630663795E-5 0.0 2.7094477630663795E-5 0.0 12-13 1.0837791052265518E-4 4.0641716445995696E-5 0.0 2.7094477630663795E-5 0.0 14-15 1.7611410459931467E-4 4.0641716445995696E-5 0.0 2.7094477630663795E-5 0.0 16-17 2.506239180836401E-4 4.0641716445995696E-5 0.0 4.0641716445995696E-5 0.0 18-19 4.4705888090595264E-4 4.0641716445995696E-5 0.0 4.0641716445995696E-5 0.0 20-21 5.012478361672802E-4 8.128343289199139E-5 0.0 5.418895526132759E-5 1.3547238815331898E-5 22-23 5.215686943902781E-4 8.128343289199139E-5 0.0 3.9286992564462503E-4 2.0320858222997845E-5 24-25 5.757576496516056E-4 8.128343289199139E-5 0.0 0.001591800560801498 3.3868097038329746E-5 26-27 6.163993660976013E-4 8.128343289199139E-5 0.0 0.0028991091064810262 4.0641716445995696E-5 28-29 6.638147019512629E-4 8.128343289199139E-5 0.0 0.010715865902927531 4.0641716445995696E-5 30-31 0.0012802140680488643 8.128343289199139E-5 0.0 0.03627950554745882 4.0641716445995696E-5 32-33 0.0013411766427178578 8.128343289199139E-5 0.0 0.08418931561788008 4.0641716445995696E-5 34-35 0.0013479502621255236 8.805705229965734E-5 0.0 0.14737363745258805 4.0641716445995696E-5 36-37 0.0014766490308711767 1.0160429111498922E-4 0.0 0.2514638468901907 4.0641716445995696E-5 38-39 0.0016527631354704916 1.0837791052265518E-4 0.0 0.41815584689344204 4.0641716445995696E-5 40-41 0.0017611410459931467 1.0837791052265518E-4 0.0 0.6084335896741861 4.0641716445995696E-5 42-43 0.0023436723150524184 1.0837791052265518E-4 0.0 0.787020065357299 4.7415335853661646E-5 44-45 0.0027229950018817116 1.0837791052265518E-4 0.0 0.993845760350971 5.418895526132759E-5 46-47 0.0027500894795123754 1.0837791052265518E-4 0.0 1.222448641740289 5.418895526132759E-5 48-49 0.0027500894795123754 1.0837791052265518E-4 0.0 1.472368103405532 8.128343289199139E-5 50-51 0.002763636718327707 1.0837791052265518E-4 0.0 1.7537984425552369 8.128343289199139E-5 52-53 0.002804278434773703 1.0837791052265518E-4 0.0 2.01547013389278 8.805705229965734E-5 54-55 0.002804278434773703 1.2192514933798708E-4 0.0 2.2770808626556542 9.483067170732328E-5 56-57 0.002804278434773703 1.2192514933798708E-4 0.0 2.5778363379754303 9.483067170732328E-5 58-59 0.002804278434773703 1.3547238815331898E-4 0.0 2.9054424406271937 9.483067170732328E-5 60-61 0.0028178256735890347 1.6256686578398278E-4 6.773619407665949E-6 3.2348435524219887 9.483067170732328E-5 62-63 0.0028178256735890347 1.6256686578398278E-4 1.3547238815331898E-5 3.567631473920617 9.483067170732328E-5 64-65 0.0028178256735890347 1.6256686578398278E-4 1.3547238815331898E-5 3.907436865125587 9.483067170732328E-5 66-67 0.0028178256735890347 1.7611410459931467E-4 1.3547238815331898E-5 4.272948141982649 9.483067170732328E-5 68-69 0.0028449201512196986 1.7611410459931467E-4 1.3547238815331898E-5 4.665411650462814 9.483067170732328E-5 70-71 0.0028584673900350303 1.7611410459931467E-4 1.3547238815331898E-5 5.093700831990125 9.483067170732328E-5 72-73 0.0028652410094426963 1.7611410459931467E-4 1.3547238815331898E-5 5.529630656209282 9.483067170732328E-5 74-75 0.0028720146288503624 1.7611410459931467E-4 1.3547238815331898E-5 5.963420016695617 9.483067170732328E-5 76-77 0.0028720146288503624 1.7611410459931467E-4 1.3547238815331898E-5 6.4174557255914655 9.483067170732328E-5 78-79 0.0028720146288503624 1.7611410459931467E-4 1.3547238815331898E-5 6.899981277715957 9.483067170732328E-5 80-81 0.0028720146288503624 1.7611410459931467E-4 1.3547238815331898E-5 7.417438385125783 9.483067170732328E-5 82-83 0.002878788248258028 1.7611410459931467E-4 1.3547238815331898E-5 7.965938990280939 9.483067170732328E-5 84-85 0.00289233548707336 1.7611410459931467E-4 1.3547238815331898E-5 8.507232464386341 9.483067170732328E-5 86-87 0.0028991091064810262 1.9643496282231252E-4 1.3547238815331898E-5 9.057169076855924 9.483067170732328E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 22805 0.0 24.203228 1 GTACTAG 2625 0.0 23.559982 1 GTATAAG 3245 0.0 21.637888 1 TAGTACT 3435 0.0 20.440756 4 TATCAAC 27435 0.0 19.915106 2 CAACGCA 24625 0.0 19.77048 5 ATCAACG 25060 0.0 19.408873 3 GTACATG 35490 0.0 19.142422 1 AACGCAG 26415 0.0 18.88843 6 TACATGG 35575 0.0 18.129309 2 TCAACGC 26980 0.0 18.079245 4 CTAGTAC 3550 0.0 17.944937 3 ATACGGG 1960 0.0 17.55599 3 ACGCAGA 27945 0.0 17.172058 7 CGCAGAG 27740 0.0 17.081047 8 ATAAGGT 2780 0.0 17.061024 3 TACGGGC 1990 0.0 17.057545 4 TCTAACG 1210 0.0 16.524668 2 TGATCGC 2380 0.0 16.41153 9 CGGGCGT 2045 0.0 16.371403 6 >>END_MODULE