FastQCFastQC Report
Fri 27 May 2016
ERR765492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7381578
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212120.2873640297508202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134540.18226455102147537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109510.1483558122666996No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT107690.1458902148023092No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA99740.13512015994412035No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA342300.036.2258031
ATCAACG432050.028.2641143
CAACGCA434350.028.0180935
TATCAAC442750.027.8331242
GGTATCA209850.027.4498141
AACGCAG460000.026.9289046
TCAACGC454600.026.9030154
GTACTAG26750.025.0476191
ACGCAGA495650.024.5792437
CGCAGAG501250.024.304648
GCAGAGT544400.022.4296179
AGTACTT315200.022.19795812-13
GAGTACT298400.020.3696612-13
AGAGTAC511650.020.3596510-11
TACTTTT354950.019.85618214-15
TCTAACG10700.018.6878492
TAGTACT34050.018.5736164
TAGGCAT40550.018.4633145
CTAACGC10950.018.2611873
GTACTTT335300.018.1765114-15
Produced by FastQC (version 0.11.5)